The organosolv pretreatment was performed on Pinus radiata chips unbiotreated or biotreated by Gloephyllum trabeum. Its effect on the structural characteristics of the material and therefore in the effectiveness of th...
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The organosolv pretreatment was performed on Pinus radiata chips unbiotreated or biotreated by Gloephyllum trabeum. Its effect on the structural characteristics of the material and therefore in the effectiveness of the enzymatic hydrolysis and fermentation was evaluated. The influences of organic solvents, reaction time, and catalyst dose on the pulp yields and composition were evaluated and 17 pulps were obtained for each material. Pulp yields ranging from 29 to 47%, and the glucan recovery was up to 79% for organosolv pulp and 81% for biorganosolv pulp. Lignin and mannan+ galactan+ xylan removal reached values over 59 and 76%, respectively. Organosolv and biorganosolv processes decreased the crystallinity (CrI) and the polymerization degree (DP) of cellulose. Enzymatic hydrolysis of pulps presented values between 64 and 96%. Decreases in DP, CrI and in lignin and hemicellulose contents resulted in a better enzymatic hydrolysis. In addition, the materials were submitted to simultaneous saccharification and fermentation. The maximum ethanol yield was 66 and 64% (% of theoretical maximum yield of ethanol) for organosolv and bio-organosolv pulp, respectively. The maximum ethanol yield was obtained from pulp pretreated under less severe conditions and using a lower enzymatic load than used in previous studies on bioethanol production from P. radiata organosolv pulps. A viable alternative to bio-organosolv process that can be used is a bio-mimetic system, similar to low- molecular complexes generated by fungi combined with organosolv process.
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