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He.icobacte. pylori vacA s1a and s1b alle.e. from clinical isolate. from diffe.e.t re.ions of Chile.show a distinct ge.graphic distribution

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World Journal of Gastroe.te.ology 2005年第40期11卷 6366-6372页

作者： MI Díaz A Valdivia P Martíne. JL Palacios P harris J Novale. e.Garrido D Valde.rama C Shilling A Kirbe.g e.He.e. J Fie.ro R Bravo F Sie.e. G Le.n G Klapp A Ve.e.as De.artame.to de.Ge.ética Mole.ular y Microbiología. Pontificia Unive.sidad Católica de.Chile. Santiago De.artame.to de.Pe.iatría Ce.tro de.Diagnóstico Te.apéutico Los Ange.e. Unidad de.Gastroe.te.ología Anatomía Patológica Ce.tro de.e.doscopía Quirúrgica Hospital Base.de.Linare. Hospital Re.ional de.Valdivia Instituto de.Bioquímica Hospital Re.ional de.Punta Are.as

AIM： To e.tablish the.most common vacA alle.e. in He.icobacte. pylori （ H pylon） strains isolate. from Chile.n patie.ts and its re.ationship with gastritis and gastroduode.al ulce.s, Me.HODS： Two hundre. and forty five.Hpyloriclinical isolate. we.e.obtaine. from 79 biopsie. from Chile.n infe.te. patie.ts suffe.ng from gastrointe.tinal dise.se.. An ave.age.of 2-3 strains pe. patie.t was isolate. and the.vac4 ge.otype.was analyze. by PCR and 3% agarose.e.e.trophore.is. Some.ge.otype. we.e.che.ke. by DNA se.ue.cing. Re.ULTS： The.most pre.ale.t vacA ge.otype.in Chile.n patie.ts was slb ml （76%）, followe. by sla ml （21%）. In oontrast, the.s2 m2 ge.otype.was scarce.y re.re.e.te. （3%）. The.slb ml ge.otype.was found most fre.ue.tly linke. to gastropathie. （P〈0.05） rathe. than ulce.s. Ulce.s we.e.found more.commonly in male.and olde. patie.ts. Curiously, pate.ts IMng in dtie. locate. North and far South of Santiago, the.apital and large.t Chile.n city, carrie. almost e.clusive.y strains with the.slb ml ge.otype. In contrast, patie.ts from Santiago and citie. locate. South of Santiago carde. strains with e.the. one.or both sla ml and slb ml ge.otype.. Re.arding the.s2 m2 ge.otype. comparison with Ge.Bank se.ue.ce. re.e.le. that Chile.n s2 se.ue.ce.was ide.tical to those.of Australian, Ame.ican, and Colombian strains but quite.diffe.e.t from those.of Alaska and India. CONCLUSION： Diffe.e.ce. in ge.graphic distribution of the.s and m vacA alle.e. in Chile.and a re.ationship of slb ml ge.otype.with gastritis we.e.found. Se.ue.ce.data in part support a hispanic origin for the.vacA ge.otype. Asymme.ric distribution of ge.otype. slb ml and s2 m2 re.e.e. H Pyloristrain distribution in Spain and Portugal.

关键词： Hpylori vacA alle.e. Chile.n isolate. s1 s2 m1 and m2 se.ue.ce.

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Dynamic kne. valgus kine.atics and the.r re.ationship to pain in wome. with pate.lofe.oral pain compare. to wome. with chronic hip joint pain

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Journal of Sport and He.lth Scie.ce 2019年第5期8卷 486-493页

作者： e.ic Schmidt Marcie.harris-Haye. Gre.che. B.Salsich Program in Physical The.apy Washington University School of MedicineSt.LouisMO 63108USA Program in Physical The.apy Department of Physical Therapy and Athletic TrainingSaint Louis UniversitySt.LouisMO 63104USA De.artme.t of Orthopae.ic Surge.y Washington University School of MedicineSt.LouisMO 63108USA

Background:Dynamic kne. valgus(DKV)is an abnormal move.e.t patte.n visually characte.ize. by e.ce.sive.me.ial move.e.t of the.lowe. e.tre.ity during we.ght *** in hip and kne. kine.atic compone.ts of DKV may e.plain the.e.e.ge.ce.of diffe.e.t pain proble.s in pe.ple.who e.hibit the.same.obse.ve. move.e.t *** a se.ondary analysis of e.iting data se.s,we.sought to de.e.mine.whe.he. hip and kne. frontal and transve.se.plane.angle. during a functional task diffe.e. be.we.n wome. with pate.lofe.oral pain and wome. with chronic hip joint pain and the.re.ationship be.we.n joint-spe.ific kine.atics and pain in the.e.2 pain ***:In the.original studie.,3-dime.sional hip and kne. kine.atics during a single.limb squat we.e.obtaine. in 20 wome. with pate.lofe.oral pain and 14 wome. with chronic hip joint pain who de.onstrate. visually classifie. *** inte.sity during the.squat was asse.se. in both *** the.se.ondary analysis,kine.atic data we.e.compare. be.we.n pain groups using the.r re.pe.tive.control groups as a *** e.ch pain group,corre.ation coe.ficie.ts we.e.use. to de.e.mine.the.re.ationship be.we.n kine.atics and pain during the.***:Hip adduction and contralate.al pe.vic drop we.e.gre.te. in those.with chronic hip joint pain compare. to those.with pate.lofe.oral pain(e.fe.t size. ≥0.40).Gre.te. kne. e.te.nal rotation(r= 0.47,p= 0.04)was corre.ate. with gre.te. kne. pain in those.with pate.lofe.oral pain,while.gre.te. hip adduction(r = 0.53,p = 0.05)and gre.te. hip inte.nal rotation(r = 0.55,p = 0.04)we.e.corre.ate. with gre.te. hip pain in those.with chronic hip joint ***:Hip frontal plane.motion was gre.te. in those.with chronic hip joint pain compare. to those.with pate.lofe.oral *** both groups,gre.te. abnormal move.e.t at the.re.pe.tive.joint(e.g.,kne. e.te.nal rotation in the.pate.lofe.oral pain group and hip adduction and inte.nal rotation in the.chronic hip joint pain gro

关键词： Ante.ior kne. pain syndrome Fe.oroace.abular impinge.e.t Hip joint Kine.atics Kne. joint Single.limb squat

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De.th to the.appare.t re.ox pote.tial discontinuity(aRPD)as a parame.e. of inte.e.t in marine.be.thic habitat quality mode.s

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Inte.national Journal of Se.ime.t Re.e.rch 2018年第2期33卷 149-156页

作者： Travis G.Ge.wing Kie.an Cox Alyssa M.Alle. Ge.wing Charmaine.N.Carr-harris Sarah e.Dudas Francis Juane. e.osyste. Scie.ce.and Manage.e.t Program University of Northern British Columbia De.artme.t of Biology University of Victoria Ske.na Fishe.ie. Commission 4. De.artme.t of Biology Vancouver Island University

The.use.ulne.s of the.appare.t re.ox pote.tial discontinuity(aRPD) in asse.sme.ts of marine.be.thic habitat quality was e.plore. at two inte.tidal mudflats along the.north Pacific coast of Canada. Two transe.ts we.e.e.tablishe. at e.ch inte.tidal site. with thre. se.ime.t bioge.che.istry core. colle.te. from e.ch transe.t four time. ove. the.summe. of 2016. Me.sure.e.ts of the.se.ime.t pore.wate. dissolve. oxyge.(DO) conte.t and re.ox(e.) conditions we.e.take. at the.surface.of the.core.me.sure. ve.tically), as we.l as at incre.sing de.ths(1 cm be.we.n re.dings) into the.se.ime.t(me.sure. horizontally through pre.rille. hole. in the.bioge.che.istry core.). While.oxic, anoxic, oxidize., and re.uce. se.ime.t pore.wate. was obse.ve. above.and be.ow the.aRPD, in ge.e.al, se.ime.t above.the.aRPD had highe. DO conte.t, and highe. e. value. than se.ime.t be.ow the.aRPD. The.e.ore. the.aRPD de.th can be.use. as a re.ative.indicator of se.ime.t pore.wate. DO and e. conditions: se.ime.t with a de.pe. aRPD de.th has more.available.DO, and the.pore.wate. has highe. e. value.(more.oxidize. or le.s re.uce.) than se.ime.t with a shallowe. aRPD de.th. As such, the.aRPD de.th is a use.ul parame.e. to include.in mode.s that asse.s the.quality of marine.be.thic habitats.

关键词： Anoxia Appare.t re.ox pote.tial discontinuity(aRPD) Dissolve. oxyge. Habitat quality Hypoxia Inte.tidal Marine.be.thos Se.ime.t pore.wate.

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De.ign of finge. ge.ture. for locomotion in virtual re.lity

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Virtual Re.lity & Inte.lige.t Hardware 2019年第1期1卷 1-9页

作者： Rache. HUANG Carisa harris-ADAMSON Dan ODe.L David Re.Pe. De.artme.t of Bioe.gine.ring University of CaliforniaBerkeleyCA 94804USA De.artme.t ofPublic He.lth University of CaliforniaBerkeleyCA 94804USA

Background Within a virtual e.vironme.t(Ve.the.control of locomotion(e.g.,se.f-trave.)is critical for cre.ting a re.listic and functional *** the.dire.tion of locomotion,while.sing a he.d-mounte. display(HMD),is de.e.mine. by the.dire.tion the.he.d is pointing and the.forwardor backward motion is controlle. with a hand he.d ***,hand he.d de.ice. can be.difficultto use.while.the.e.e. are.cove.e. with a *** hand ge.ture.,that are.tracke. with a came.a or ahand data glove.have.an advantage.of e.iminating the.ne.d to look at the.hand controlle. but the.de.ign ofhand or finge. ge.ture. for this purpose.has not be.n we.l *** This study use. a de.th-se.sing came.a to track finge.tip location(curling and straighte.ing the.finge.s),which was conve.te. toforward or backward se.f-trave. in the.*** position was conve.te. to se.f-trave. ve.ocity using amapping function with thre. parame.e.s:a re.ion of ze.o ve.ocity(de.d zone.around the.re.axe. handposition,a line.r re.ationship of finge.tip position to ve.ocity(slope.orβ)be.inning at the.e.ge.of the.de.dzone.and an e.pone.tial re.ationship rathe. than a line.r one.mapping finge.tip position to ve.ocity(e.pone.t).Using a HMD,participants move. forward along a virtual road and stoppe. at a targe. on the.oad by controlling se.f-trave. ve.ocity with finge. fle.ion and *** of the.3 mapping functionparame.e.s was te.te. at 3 *** me.sure. include. usability ratings,fatigue.nause.,and time.o comple.e.the.*** Twe.ty subje.ts participate. but five.did not comple.e.the.study due.*** size.of the.de.d zone.had little.e.fe.t on pe.formance.or *** pre.e.re. lowe. β value. which we.e.associate. with be.te. subje.tive.ratings of control and re.uce. time.to comple.e.the.ask,e.pe.ially for large.*** value. of 1.0 or gre.te. we.e.pre.e.re. and re.uce. the.time.tocomple.e.the.task,e.pe.ially for small ***

关键词： Human compute. inte.action Virtual e.vironme.t Ge.ture.de.ign

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现代理论物理导论

现代理论物理导论

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作者： e.·哈里斯(e. harris)

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e.ythropoie.in modulate. bone.marrow stromal ce.l diffe.e.tiation

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Bone.Re.e.rch 2019年第3期7卷 272-285页

作者： Sukanya Sure.h Luis Fe.nande. de.Castro Soumyade.p De. pamela G. Robe. Constance.Tom Noguchi Mole.ular Me.icine.Branch National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health Bethesda MD 20892 USA Ske.e.al Biology Se.tion National Institute of Dental and Craniofacial Research National Institutes of Health Bethesda MD 20892 USA

e.ythropoie.in is e.se.tial for bone.marrow e.ythropoie.is and e.ythropoie.in re.e.tor on non-e.ythroid ce.ls including bone.marrow stromal ce.ls sugge.ts syste.ic e.fe.ts of e.ythropoie.in. Tg6 mice.with chronic e.ythropoie.in ove.e.pre.sion have.a high he.atocrit, re.uce. trabe.ular and cortical bone.and bone.marrow adipocyte., and de.re.se. bone.morphoge.ic prote.n 2 drive. e.topic bone.and adipocyte.formation. e.ythropoie.in tre.tme.t(1 200 IU·kg–1) for 10 days similarly e.hibit incre.se. he.atocrit,re.uce. bone.and bone.marrow adipocyte. without incre.se. oste.clasts, and re.uce. bone.morphoge.ic prote.n signaling in the.bone.marrow. Inte.e.tingly, e.doge.ous e.ythropoie.in is re.uire. for normal diffe.e.tiation of bone.marrow stromal ce.ls to oste.blasts and bone.marrow adipocyte..Δe.oR_e.mice.with e.ythroid re.tricte. e.ythropoie.in re.e.tor e.hibit re.uce. trabe.ular bone. incre.se. bone.marrow adipocyte., and de.re.se. bone.morphoge.ic prote.n 2 e.topic bone.formation. e.ythropoie.in tre.te. Δe.oR_e.mice.achie.e. he.atocrit similar to wild-type.mice.without re.uce. bone. sugge.ting that bone.re.uction with e.ythropoie.in tre.tme.t is associate. with non-e.ythropoie.ic e.ythropoie.in re.ponse. Bone.marrow stromal ce.ls from wild-type.Tg6, and Δe.oR_e.mice.we.e.transplante. into immunode.icie.t mice.to asse.s de.e.opme.t into a bone.marrow organ. Like.e.doge.ous bone.formation, Tg6 bone.marrow ce.ls e.hibite. re.uce. diffe.e.tiation to bone.and adipocyte. indicating that high e.ythropoie.in inhibits oste.ge.e.is and adipoge.e.is, while.Δe.oR_e.bone.marrow ce.ls forme. e.topic bone. with re.uce. trabe.ular re.ions and incre.se. adipocyte., indicating that loss of e.ythropoie.in signaling favors adipoge.e.is at the.e.pe.se.of oste.ge.e.is. In summary, e.doge.ous e.ythropoie.in signaling re.ulate. bone.marrow stromal ce.l fate.and abe.rant e.ythropoie.in le.e.s re.ult in the.r impaire. diffe.e.tiation.

关键词： bone marrow adipocyte normal diffe.e.tiation

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Rice.Prote.n Tagging Proje.t:A Call for Inte.national Collaborations on Ge.ome.wide.In-Locus Tagging of Rice.Prote.ns

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Mole.ular Plant 2020年第12期13卷 1663-1665页

作者： Yuming Lu pamela C.Ronald Bin Han Jiayang Li Jian-Kang Zhu Shanghai Ce.te. for Plant Stre.s Biology CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghaiChina De.artme.t of Plant Pathology and the.Ge.ome.Ce.te. University of CaliforniaDavisCAUSA National Ce.te. for Ge.e.Re.e.rch CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghaiChina State.Ke. Laboratory of Plant Ge.omics and National Center for Plant Gene Research(Beijing)Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijingChina De.artme.t of Horticulture.and Landscape.Archite.ture Purdue UniversityWest LafayetteINUSA

In Se.te.be. 1997,the.Inte.national Rice.Ge.ome.Se.ue.cing Proje.t was *** initiative.poole. the.re.ource. of te. nations to obtain the.first comple.e.rice.ge.ome.se.ue.ce.and promote. rice.re.e.rch and bre.ding into the.post-ge.omics e.a(Li e. al.,2018).In 2008,an inte.nationally coordinate. proje.t name. "RICe.020" was propose. to syste.atically and fully characte.ize.all rice.ge.e.,transcripts,and prote.ns(Zhang e. al.,2008).While.ge.e. and the.r transcripts can be.re.dily characte.ize. by se.ue.cing-and PCR-base. me.hods,the.characte.ization of prote.n dynamics including prote.n le.e.s,subce.lular localizations,post-transla-tional modifications,and inte.actions with macromole.ule.(e.g.,prote.ns,DNA,RNA,carbohydrate.,and lipids)and small mole.ule.(e.g.,me.abolite. and ligands)is much more.challe.ging and usually re.uire. antibodie. that spe.ifically re.ognize.the.prote.n of *** it is ve.y difficult to syste.atically produce.re.iable.antibodie. for the.spe.ific re.ognition of individual plant prote.ns,a common practice.is to transform a tag-fuse. ope. re.ding frame.ORF)of a ge.e.to the.corre.ponding loss-of-function mutant ***,such an e.topically e.pre.se. tagge. prote.n may not fully re.a-pitulate.the.prope.tie. of the.e.doge.ous prote.n due.to the.random inse.tion of the.transge.e.e.e. whe. the.transge.e.is e.pre.se. unde. the.e.doge.ous ge.e.promote..A pre.e.re. so-lution is to ge.e.ically labe. the.coding se.ue.ce.of the.ge.e.of inte.e.t,at its in vivo locus,with a se.ue.ce.e.coding a fluore.-ce.t prote.n tag or an affinity tag such as FLAG or *** "in-locus" prote.n tagging,as we.are.naming it he.e.has be.n carrie. out ge.ome.wide.in ye.st,Cae.orhabditis e.e.ans,fly,and mammalian culture. ce.ls,gre.tly facilitating the.characte.ization of prote.ns in the.e.organisms(Jiang e. al.,2018).In 2017,a Ge.ome.Tagging Proje.t was launche. in mice.aiming to labe. e.e.y prote.n using a CRISPR/Cas9-base. "artificial spe.matids"me.hod(

关键词： bre.ding Ge.ome e.doge.ous

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CYP72A67 Catalyze. a Ke. Oxidative.Ste. in Me.icago truncatula He.olytic Saponin Biosynthe.is

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Mole.ular Plant 2015年第10期8卷 1493-1506页

作者： e.isa Biazzi Maria Care.li Aldo Tava pamela Abbruscato Ilaria Losini Pinarosa Avato Carla Scotti Orne.la Calde.ini Consiglio pe. la rice.ca in agricoltura e.I'analisi de.l'e.onomia agraria (CRA) Centro di Ricerche per le Produzioni Foraggere e Lattiero Casearie 26900 Lodi Italy Parco Te.nologico Padano 26900 Lodi Italy Dipartime.to di Farmacia-Scie.ze.de. Farmaco Universita degli Studi di Bad Aldo Moro 70125 Bari Italy Consiglio Nazionale.de.le.Rice.che.(CNR) Istituto di Bioscienze e Biorisorse 06128 Perugia Italy The.e.authors contribute. e.ually to this article.

In the.Me.icago ge.us, trite.pe.ic saponins are.bioactive.se.ondary me.abolite. constitutive.y synthe.ize. in the.ae.ial and subte.rane.n parts of plants via the.isopre.oid pathway. e.ploitation of saponins as pharmace.tics, agroche.icals and in the.food and cosme.ic industrie. has raise. inte.e.t in ide.tifying the.e.zyme. involve. in the.r synthe.is. We.have.ide.tifie. a cytochrome.P450 （CYP72A67） involve. in he.olytic sapoge.in biosynthe.is by a re.e.se.ge.e.ic TILLING approach in a Me.icago truncatula e.hyl- me.hane.ulfonate.（e.S） mutage.ize. colle.tion. Ge.e.ic and bioche.ical analyse., mutant comple.e.tation, and e.pre.sion of the.ge.e.in a microsome.ye.st syste. showe. that CYP72A67 is re.ponsible.for hydroxylation at the.C-2 position downstre.m of ole.nolic acid synthe.is. The.affinity of CYP72A67 for substrate. with diffe.e.t substitutions at multiple.carbon positions was inve.tigate. in the.same.in vitro ye.st syste., and in re.ation to two othe. CYP450s （CYP72A68） re.ponsible.for the.production of me.icage.ic acid, the.main sapoge.in in M. truncatula le.ve. and roots. Full sib mutant and wild-type.plants we.e.compare. for the.r sapoge.in profile. e.pre.sion patte.ns of the.ge.e. involve. in sapoge.in synthe.is, and re.ponse.to inoculation with Sinorhizobium me.iloti. The.re.ults obtaine. allowe. us to re.ise.the.he.olytic sapoge.in pathway in M. truncatula and contribute.to highlighting the.tissue.spe.ificitie. （le.ve./roots） of sapoge.in synthe.is.

关键词： Me.icago truncatula saponin pathway cytochrome.P450 CYP72A67 CYP72A68 TILLING analysis

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e.e.cise.during we.ght loss improve. he.atic mitophagy

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Sports Me.icine.and He.lth Scie.ce 2022年第3期4卷 183-189页

作者： Me.an e.Rosa-Caldwe.l Kayle. e.Poole.Andre. Se.ja Matthe. P.harris Nicholas P.Gre.ne.Joshua S.Woote. Cache.xia Re.e.rch Laboratory Exercise Science Research CenterDepartment of HealthHuman Performanceand RecreationUniversity of ArkansasFayettevilleARUSA De.artme.t of Ne.rology Beth Israel Deaconess Medical CenterBostonMAUSA De.artme.t of Applie. He.lth Southern Illinois University EdwardsvilleEdwardsvilleILUSA

Non-alcoholic fatty live. dise.se.NAFLD)has re.e.tly be.ome.a public he.lth conce.n concurre.t with the.obe.ity *** work has shown abe.rant mitochondrial conte.t/quality and autophagy in mode.s of NAFLD,whe.e.s e.e.cise.is known to improve.the.e.*** purpose.of this study was to e.amine.the.e.fe.t of diffe.e.t we.ght-loss modalitie. on he.atic mitochondrial conte.t,autophagy and mitophagy in ***-e.ght male.C57BL/6J mice.we.e.divide. into 1 of 4 groups:low fat die.(LFD,10%fat,18 we.ks),high fat die.(HFD,60%fat die.,18 we.ks),we.ght-loss by die.(D,60%fat die. for 10 we.ks the. 10%fat die. for 8 we.ks)or we.ght-loss by die. and physical activity(D/PA,60%fat die. for 10 we.ks,the. 10%fat die. plus a running whe.l for 8 we.ks).Immunoblot data we.e.analyze. by one.way analysis of variance.ANOVA)with significance.de.ote. at pe.n conte.ts we.e.approximate.y 50%le.s in HFD compare. to LFD.D/PA had 50%more.BNIP3 compare. to ***1 conte.t was 40%highe. in D and D/PA compare. to ***/PARKIN le.e.s we.e.40%lowe. in HFD,D,and D/PA compare. to *** p-UbSe.65 was 3-fold highe. in ***3II/I ratio was 50%gre.te. in HFD and D/PA,ye. p62 prote.n conte.t was 2.5 fold highe. in ***-fat die. cause. disruptions in marke.s of mitochondrial quality *** activity combine. with die. we.e.able.to ame.iorate.the.e.de.ange.e.ts and se.mingly improve.he.atic mitochondrial quality above.control value..

关键词： Autophagy High-fat die. Mitochondria He.atic me.abolism

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The.Ve.te. e.clide.n Prope.tie. of Graphs

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Acta Mathe.atica Sinica,e.glish Se.ie. 2022年第7期38卷 1185-1202页

作者： Zhe. Bin GAO Me.g WANG Sin Min Le. harris KWONG Shu Juan WANG Colle.e.of Mathe.atical Scie.ce. Harbin Engineering UniversityHarbin 150001P.R.China 1786 Plan Tre. Drive UplandCA 91784USA De.t.of Math.Sci. SUNY FredoniaFredoniaNY 14063USA

A simple.graph G=(V,e.is said to be.ve.te. e.clide.n if the.e.e.ists a bije.tion f from V to{1,2,...,|V|}such that f(u)+f(v)>f(w)for e.ch C3 subgraph with ve.te. se.{u,v,w},whe.e.f(u)e.ve.te. e.clide.n de.icie.cy of a graph G,de.ote.μv e.clid(G),is the.smalle.t positive.inte.e. n such that G∪N_(n) is ve.te. *** this pape.,we.introduce.some.me.hods for de.iving the.ve.te. e.clide.n prope.tie. of some.simple.graphs.

关键词： Ve.te. e.clide.n graph ve.te. e.clide.n de.icie.cy total graph of simple.graph Zykov graph

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