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Genome-Wide Sequencing of 41 Rice （Oryza sativa L.） Mutated Lines Reveals Diverse Mutations Induced by Fast-Neutron Irradiation

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Molecular Plant 2016年第7期9卷 1078-1081页

作者： Li, Guotian chern, Mawsheng Jain, Rashmi Martin, Joel A. Schackwitz, Wendy S. Jiang, Liangrong Vega-Sanchez, Miguel E. Lipzen, Anna M. Barry, Kerrie W. Schmutz, Jeremy Ronald, pamela c. Univ calif Davis Dept Plant Pathol Davis CA 95616 USA Univ calif Davis Genome Ctr Davis CA 95616 USA Lawrence Berkeley Natl Lab Feedstocks Div Joint BioEnergy Inst Berkeley CA 94720 USA US DOE Joint Genome Inst Walnut Creek CA 94598 USA Xiamen Univ Sch Life Sci Xiamen 361102 Peoples R China HudsonAlpha Inst Biotechnol Huntsville AL 35806 USA Monsanto co Chesterfield Village Campus Chesterfield MO 63017 USA

Fast-neutron （FN） irradiation has been used to create mutagen- ized collections of many plant species （Bolon et al., 2014）, FN- induced mutagenesis has clear advantages： it is an efficient means of saturating the genome, and it does not involve time- consuming plant transformation or tissue culture. In rice, most mutant collections, although highly valuable, were generated us- ing either T-DNA insertion or transposon tagging approaches that often induce mutations unlinked to the insertion and complicating analysis （Wang et al., 2013）. Another disadvantage of some of these collections is that they were created in rice varieties that are photoperiod-sensitive and often have long generation times.

关键词： mutagenesis genome valuable

Temporal and spatial comparisons of angiosperm diversity between eastern Asia and North America

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National Science Review 2022年第6期9卷 38-42页

作者： Haihua Hu Jianfei Ye Bing Liu Lingfeng Mao Stephen A.Smith Russell L.Barrett pamela S.Soltis Douglas E.Soltis Zhiduan chen Limin Lu State Key Laboratory of Systematic and Evolutionary Botany Institute of Botany Chinese Academy of Sciences University of chinese Academy of Sciences Sino-Africa Joint Research center Chinese Academy of Sciences Beijing Botanical Garden Institute of BotanyChinese Academy of Sciences co-Innovation center for Sustainable Forestry in Southern china College of Biology and the Environment Nanjing Forestry University Department of Ecology and Evolutionary Biology University of Michigan National Herbarium of New South Wales Royal Botanic Gardens and Domain Trust Florida Museum of Natural History University of Florida Department of Biology University of Florida

comparative approaches that examine floras with similar environments, but different diversity patterns, provide good opportunities to elucidate how uneven distributions of biodiversity were assembled. Eastern Asia(EA) and North America north of Mexico(NA; see definitions of EA and NA in Fig. S1 A) share similar geographical environments with comparable latitude, land area and climate,

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Radio Pet Therapy of Healthy Wistar Rats with 64cucl2Labeled to 64cu(AcAc)2Supported in Functionalized TiO2Nanoparticles

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Journal of Encapsulation and Adsorption Sciences 2017年第3期7卷 121-126页

作者： Tessy López Juan c. Manrique-Arias Paola Ramírez pamela Larraza Francisco Rodríguez Reinoso Departamento de Atención a la Salud Universidad Autónoma Metropolitana-Xochimilco CdMx México Unidad Radiofarmacia-ciclotrón Facultad de Medicina Universidad Nacional Autónoma de México CdMx México Departamento de Química Universidad de Alicante Espa&ntilde a

In recent years copper-64 in the chemical form of copper chloride ([64cu]cucl2) has been identified as a potential agent for PET imaging and radionuclide therapy. The aim of this research was to determine the biodistribution and the labeling of radio nanoparticle with 64cucl2, the nanoparticles cu(acac)2/F-TiO2 were mixed with 64cucl2 (20-37 MBq), then the final solution was used to injected healthy Wistar rats to probe the absorption of nanoparticle inside tissue trough of the groups OH that are formed during the functionalization of the cu(acac)2/F-TiO2. The “in vivo” evaluation after realize the images study in micro PET equipment, uptake of the radio-nanoparticle was observed in the digestive system in the healthy Wistar rats.

关键词： PET Therapy Nanoparticles Functionalized Titania (F-Tio2) Titanium Acetylacetonate [cu (AcAc)2] cu(Acac)2/F-Tio2 PET Lesion Detection Imaging

Design and Implementation of Low-Pass, High-Pass and Band-Pass Finite Impulse Response (FIR) Filters Using FPGA

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circuits and Systems 2015年第2期6卷 30-48页

作者： Emmanuel S. Kolawole Warsame H. Ali Penrose cofie John Fuller c. Tolliver pamela Obiomon Department of Electrical and computer Engineering Prairie View A&M University Prairie View USA

This paper presents the design and implementation of a low-pass, high-pass and a hand-pass Finite Impulse Response (FIR) Filter using SPARTAN-6 Field Programmable Gate Array (FPGA) device. The filter performance is tested using Filter Design and Analysis (FDA) and FIR tools from Mathworks. The FDA Tool is used to define the filter order and coefficients, and the FIR tool is used for Simulink simulation. The FPGA implementation is carried out using Spartan-6 LX75T-3FGG676c for different filter specifications and simulated with the help of Xilinx ISE (Integrated Software Environment). System Generator ISE design suit 14.6i is used in synthesizing and co-simulation for FPGA filter output verification. Finally, comparison is done between the results obtained from the software simulations and those from FPGA using hardware co-simulation. The simulation waveforms and synthesis reports verify the parallel implementation of FPGA which proves its effectiveness in terms of speed, resource usage and power consumption.

关键词： Digital Filters FIR Filter Matlab Simulink FDA Tool FIR Tool Distributed Arithmetic FPGA Xilinx System Generator

Spatio-Temporal Pulsating Dissipative Solitons through collective Variable Methods

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Journal of Applied Mathematics and Physics 2016年第6期4卷 1032-1041页

作者： Olivier Asseu Ambroise Diby pamela Yoboué Aladji Kamagaté Ecole Supérieure Africaine des Technologies de l’Information et de communication (ESATIc) Abidjan Cote d’Ivoire Department of Electrical and Electronic Engineering Institut National Polytechnique Houphouet Boigny (INPHB) Yamoussoukro C&ocircte d’Ivoire UFR des Sciences des Structure de la Matière et de Technologie de l’Université Félix Houphouet Boigny Abidjan Cote d’Ivoire

A semi-analytical approach for the pulsating solutions of the 3D complex cubic-quintic Ginzburg-Landau Equation (cGLE) is presented in this article. A collective variable approach is used to obtain a system of variational equations which give the evolution of the light pulses parameters as a function of the propagation distance. The collective coordinate approach is incomparably faster than the direct numerical simulation of the propagation equation. This allows us to obtain, efficiently, a global mapping of the 3D pulsating soliton. In addition it allows describing the influence of the parameters of the equation on the various physical parameters of the pulse and their dynamics.

关键词： Dissipative Soliton Pulsating Light Pulse Spatiotemporal Pulses collective Variable Approach complex cubic-Quintic Ginzburg-Landau Equation Bifurcation

Phylogenetic diversity of eastern Asia-eastern North America disjunct plants is mainly associated with divergence time

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Plant Diversity 2023年第1期45卷 27-35页

作者： Han-Yang Lin Miao Sun Ya-Jun Hao Daijiang Li Matthew A.Gitzendanner cheng-Xin Fu Douglas E.Soltis pamela S.Soltis Yun-Peng Zhao Laboratory of Systematic and Evolutionary Botany and Biodiversity College of Life SciencesZhejiang UniversityHangzhou 310058China Key Laboratory of Horticultural Plant Biology(Ministry of Education) Huazhong Agricultural UniversityWuhan 430070China Florida Museum of Natural History University of FloridaGainesvilleFL 32611USA Department of Biology University of FloridaGainesvilleFL 32611USA Biodiversity Institute University of FloridaGainesvilleFL 32611USA Genetics Institute University of FloridaGainesvilleFL 32608USA School of Advanced Study Taizhou UniversityTaizhou 318000China

The underlying causes of biodiversity disparities among geographic regions have long been a fundamental theme in ecology and ***,the patterns of phylogenetic diversity(PD) and phylogenetic beta diversity(PBD) of congeners that are disjunctly distributed between eastern Asia-eastern North America(EA-ENA disjuncts) and their associated factors remain *** we investigated the standardized effect size of PD(SES-PD),PBD,and potentially associated factors in 11natural mixed forest sites(five in EA and six in ENA) where abundant EA-ENA disjuncts *** found that the disjuncts in ENA possessed higher SES-PD than those in EA at the continental scale(1.96vs-1.12),even though the number of disjunct species in ENA is much lower than in EA(128 vs 263).SESPD of the EA-ENA disjuncts tended to decrease with increasing latitude in 11 *** latitudinal diversity gradient of SES-PD was stronger in EA sites than in ENA *** on the unweighted unique fraction metric(UniFrac) distance and the phylogenetic community dissimilarity,PBD showed that the two northern sites in EA were more similar to the six-site ENA group than to the remaining southern EA *** on the standardized effect size of mean pairwise distances(SES-MPD),nine of eleven studied sites showed a neutral community structure(-1.96 ≤SES-MPD ≤1.96).Both Pearson’s r and structural equation modeling suggested that SES-PD of the EA-ENA disjuncts was mostly associated with mean divergence ***,SES-PD of the EA-ENA disjuncts was positively correlated with temperaturerelated climatic factors,although negatively correlated with mean diversification rate and community *** applying approaches from phylogenetics and community ecology,our work sheds light on historical patterns of the EA-ENA disjunction and paves the way for further research.

关键词： EA-ENA disjuncts climatic factors community assembly processes Diversification rate Divergence time Phylogenetic diversity

Effect of copper on Growth characteristics and Disease control of the Recently Introduced Guignardia citricarpaon citrus in Florida

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American Journal of Plant Sciences 2013年第2期4卷 282-290页

作者： Katherine E. M. Hendricks Ryan S. Donahoo pamela D. Roberts Mary c. christman Mcc Statistical consulting LLc Gainesville USA Southwest Florida Research and Education center University of Florida-IFAS Immokalee USA

Guignardia citricarpa, the plant pathogenic fungus that causes citrus black spot, was recently introduced into the United States. The development of this disease in the presence of multiple applications of copper per year to manage citrus canker warrants an investigation into the effects of copper on growth of isolates of G. citricarpa from citrus in Florida. Guignardia citricarpa and G. mangiferae isolates, confirmed by internal transcribed spacer (ITS) sequencing of ribosomal DNA and DNA homology, were inoculated on non-amended media and media amended with 50 and 500 μg·ml-1 copper sulfate. Radial colony growth was assessed over a 26 to 59 day period. copper reduced the growth of G. citricarpa isolates in media amended with 500 μg·ml-1 copper but had variable effects on radial growth in media amended with 50 μg·ml-1 copper. There was little effect of copper on the in vitro growth of G. mangiferae isolates. Field application of copper with and without an adjuvant for the control of citrus black spot was undertaken in a commercial grove in Florida in 2011. Spray applications were made on a 23.3 ± 4.7 day interval and fruit accessed between December 2011 and March 2012 for black spot symptoms. copper failed to reduce the proportion of fruit exhibiting symptoms compared to that of the controls.

关键词： copper Tolerance citrus Black Spot Guignardia mangiferae Saprophyte citrus x sinensis

Progressive familial intrahepatic cholestasis—farnesoid X receptor deficiency due to NR1H4 mutation:A case report

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World Journal of clinical cases 2021年第15期9卷 3631-3636页

作者： Piotr czubkowski Richard J Thompson Irena Jankowska A S Knisely Milton Finegold pamela Parsons Joanna cielecka-Kuszyk Sandra Strautnieks Joanna Pawłowska Laura N Bull Department of Gastroenterology HepatologyNutritional Disorders and PediatricsThe Children’s Memorial Health InstituteWarsaw 04-730Poland Institute of Liver Studies King's College London HospitalLondon SE59RSUnited Kingdom Institut für Pathologie Medizinische Universität GrazGraz 8010Austria Department of Pathology and Immunology Baylor College of MedicineHoustonTX 77030United States Texas children’s Hospital HoustonTX 77030United States Department of Pathology The Children’s Memorial Health InstituteWarsaw 04-730Poland Department of Medicine and Institute for Human Genetics UCSF Liver Center LaboratoryUniversity of California San FranciscoSan FranciscoCA 94143United States

BAcKGROUND Functioning farnesoid X receptor(FXR;encoded by NR1H4)is key to normal bile acid *** mutations in NR1H4 are reported in a few children with intrahepatic *** describe a boy with progressive familial intrahepatic cholestasis and homozygous mutation in *** SUMMARY A boy had severe neonatal cholestasis with moderate hypercholanemia and persistently elevated *** medical treatment,coagulopathy was uncontrollable,prompting liver transplantation at age 8 mo with incidental *** patient experienced catch-up growth with good liver function and did not develop allograft ***,1 year after transplant,he died from an acute infection,considered secondary to immunosuppression and asplenia.A homozygous protein-truncating mutation,c.547c>T,p.(Arg183Ter),was subsequently identified in NR1H4,and both parents were shown to be heterozygous *** of FXR and of bile salt export pump expression was confirmed by immunostaining of explanted *** Severe cholestasis with persistently high alpha-fetoprotein and modest elevation of serum bile acid levels may suggest FXR *** patients with FXR deficiency may not develop allograft steatosis and may respond well to liver transplantation.

关键词： Neonatal cholestasis Progressive familial intrahepatic cholestasis Bile salt export pump Liver transplantation Alpha-fetoprotein case report

carotenoids,Polyphenols and Antioxidant Activity Evaluation in Stone-Grinded Wheat Semolina

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Journal of Health Science 2018年第6期6卷 432-438页

作者： pamela Vignolini Silvia Urciuoli Daniela Heimler Annalisa Romani Laboratorio Phytolab DiSIADipartimento di StatisticaInformaticaApplicazioni“Giuseppe Parenti”Universitàdegli Studi di FirenzePolo Scientifico e Tecnologico via U.Schiff6-Sesto Fiorentino(FI)50019Italy PIN S.c.r.l.-Servizi Didattici e Scientifici per l’Universitàdi Firenze Piazza G.Ciardi25Prato 59100Italy

The functional quality of stone grinded grain semolina,also related to the presence of secondary antioxidant metabolites,was evaluated by HPLc/DAD(high performance liquid chromatography/diode array detection)analysis and by in vitro spectrophotometric *** total polyphenol content was determined with the Folin-ciocalteau spectrophotometric method,while carotenoid content evaluation was performed by HPLc/DAD chromatographic analysis:the presence of lutein as the main compound ranging between 75 and 78.5%of total carotenoids was pointed *** chromatographic analyses identified the presence of an indolacetic acid derivative whose content changed between 0.097 and 0.108 mg/g.

关键词： HPLc/DAD lutein Folin-ciocalteau flavonoids.