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Immunophenotyping of peripheral blood mononuclear cells and intracellular detection of IL-2, IFNγ and IL-4 by flow cytometry in patients with actinomycetoma by Nocardia brasiliensis and Actinomadura madurae. Findings in six patients

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Health 2011年第3期3卷 172-178页

作者： Heuze de Icaza Yvonne Castrillón Rivera Laura Estela Garibay-Escobar Adriana Santos-Argumedo Leopoldo Sandoval Trujillo Horacio Padilla Desgarennes Carmen palma ramos alejandro 不详

Mycetoma is a chronic, granulomatous, progressive inflammatory disease;it usually involves the subcutaneous tissue after a traumatic inoculation of the causative organism. Mycetoma may be caused by true fungi or bacteria (actinomycetes) and hence it is classified as eumycetoma and actinomycetoma respectively. Mycetoma immunological studies have fre- quently addressed humoral aspects. Few reports have addressed the role of cellular immunity in humans, for this reason, we were interested in finding differences in the circulating population of mononuclear cells or in the ability of this pathology to produce cytokines after mitogen stimulation. In this study, immunophenotyping of peripheral blood mononuclear cells (PBMN) and intracellular detection by flow cytometry of IL-2, IFNγ and IL-4 in patients with actino- mycetoma by Nocardia brasiliensis and Actino- madura madurae were evaluated. We investi- gated the expression of T-cells (CD3, CD4 and CD8), B-cells (CD19), monocytes (CD14), and natural killer cells (CD16 and CD56) markers. CD69 and CD25 were used to monitor individual activated cell subsets. The percentage values of the cells were calculated. Our results indicated that PBMC from patients with mycetoma show similar percentages of circulating cells when compared with healthy donors. The expression of IL-2 receptor (IL-2R) by mitogen activation was similar in these two groups. These findings suggest that circulating lymphocytes are not affected by this pathology. Intracellular IL-4 was increased only in patients with mycetoma by N. brasiliensis, suggesting a TH2 profile;this ob- servation has been reported by other authors.

关键词： Actinomycetoma Nocardia brasiliensis Actinomadura madurae Cytokines CD4+ CD8+ CD56+

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Spectral Properties and Optimality for Elementary Matrices

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Journal of the Operations Research Society of China 2018年第3期6卷 467-472页

作者： Ricardo Biloti João Daniel palma ramos Jin-Yun Yuan Department of Applied Mathematics University of Campinas&INCT-GPCampinas 13083-859Brazil Instituto Kumon de Educação Ltda. São Paulo 04006-002Brazil Department of Mathematics Federal University of ParanáCuritiba 81531-980Brazil

A generalization of the Householder transformation,renamed as elementary matrix by ***:Unitary transformation of a nonsymmetric matrix,***,5(4),339–342,1958,was introduced by LaBudde(Math Comput 17(84):433–437,1963)as a tool to obtain a tridiagonal matrix similar to a given square *** of the free parameters of the transformation can be chosen to attain better numerical *** this work,we study the spectral properties of the *** also propose a special choice for free coefficients of that transformation to minimize its condition *** transformation with such suitable choice of parameters is called optimal.

关键词： Symmetric and triangular(ST)decomposition Non-symmetric system Elementary matrix Householder transformation

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Assessment of Beauveria bassiana and Their Enzymatic Extracts against Metamasius spinolae and Cyclocephala lunulata in Laboratory

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Advances in Enzyme Research 2016年第3期4卷 98-112页

作者： Lluvia Sánchez-Pérez Silvia Rodríguez-Navarro Victor Hugo Marín-Cruz Miguel Ángel ramos-López alejandro palma ramos Juan Esteban Barranco-Florido Masters Program in Agricultural Sciences Division of Biological and Health Metropolitan Autonomous University-Xochimilco Coyoacán México Departament of Agricultural and Animal Production Metropolitan Autonomous University-Xochimilco Coyoacán México Faculty of Chemistry Autonomous University of Queretaro Santiago de Querétaro México Department of Biological Systems Metropolitan Autonomous University-Xochimilco Coyoacán México

The application of enzymatic extracts and conidia of Beauveria bassiana in Metamasius spinolae and Cyclocephala lunulata was evaluated. The variables were mortality and time of death. In M. spinolae, mortality with extracts 29%, conidia 27% and the combination of both 31%, all had a time of death of four days. Although with different symptoms, used enzymatic extracts: contraction and softening of the joints;by conidia: mycelium in the joints;in the combination of conidia and enzymatic extracts: abundant aerial mycelium. In C. lunulata, 100% mortality in all treatments;Time of death: enzymatic extracts and extracts with conidia 1.2 days;conidia 2.8 days. Symptoms were different, enzymatic extracts: melanization and internal tissue lysis;enzymatic extract and conidia: mycelium emerged and melanization;conidia: mycelium emerged. Enzymatic extracts showed insecticidal activity in M. spinolae and C. lunulata. These results suggest the potential of enzymatic extracts as biocontrol agents to improve the use of entomopathogenic fungi.

关键词： Insecticidal Activity Beauveria bassiana Metamasius spinolae Cyclocephala lunulata Enzymatic Extracts

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Stimulation of the Protective Response to Actinomycetoma by Nocardia brasiliensis in Mice Treated with Candida albicans Antigens

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Advances in Microbiology 2014年第6期4卷 297-305页

作者： alejandro palma-ramos Violeta Karen Espinosa-Antunez Laura Estela Castrillón-Rivera Oralia Nájera-Medina María Elisa Vega-Memije Roberto Arenas-Guzmán María Elisa Drago-Serrano Teresita Sainz-Espunes Department of Biological Systems Universidad Autónoma Metropolitana-XochimilcoMexico CityMexico Department of Health Care Universidad Autónoma Metropolitana-XochimilcoMexico CityMexico General Hospital“Manuel Gea González”Dermatology Service Mexico City Health SecretaryMexico CityMexico General Hospital“Manuel Gea González”Mycology Service Mexico City Health SecretaryMexico CityMexico

Actinomycetoma is a chronic subcutaneous lesion caused by the pathogenic bacterium Nocardia (N.) brasiliensis. Yeast antigens of Candida (C.) albicans increase the interferon (IFN)-γ and TCD4+ cells levels that enhance the phagocytic killing of N. brasiliensis, able to survive inside phagocytes and to grow in clustered colonies that form grains. The aim of this work was to test the effect of a crude protein extract of C. albicans on the levels of IFN-γ producing TCD4+ cells and on the outcome of actinomycetoma lesion. Five BALB/c mice with N. brasiliensis infection at left hind footpad were treated four times every other day with C. albicans crude protein extract (CPE). Five uninfected mice treated with CPE or infected mice treated with sterile phosphate-saline buffer were included as positive and negative control groups, respectively. Footpad thickness was recorded in all groups. Once the treatments were finished, single cell suspensions from blood and spleen were prepared for assessing the amount (%) of IFN-γ producing TCD4+ cells by cytofluorometry;presence of TCD4+ and IFN-γ in footpad sections was detected by immunofluorescence and immunohistochemistry, respectively. By comparison with the negative control group, infected mice treated with CPE had lower footpad thickness, higher percentage of blood and spleen IFN-γ producing TCD4+ cells as well as in situ presence of IFN-γ and TCD4+ cells. These findings showed that CPE from C. albicans displayed an immunoadjuvant activity that enhanced the presence of IFN-γ producing TCD4+ cells and IFN-γ for the resolution of N. brasiliensis actinomycetoma in mice.

关键词： Actinomycetoma Nocardiabrasilensis Candidaalbicans

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Complementarity of electric vehicles and pumped-hydro as energy storage in small isolated energy systems:case of La palma,Canary Islands

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Journal of Modern Power Systems and Clean Energy 2016年第4期4卷 604-614页

作者： Alfredo RAMIREZ-DIAZ Francisco J.ramos-REAL Gustavo A.MARRERO Departamento de Economı´a Contabilidad y FinanzasFacultad de Economı´aEmpresa y TurismoUniversidad de La Laguna38071 La LagunaSpain Instituto Universitario de Ciencias Polı´ticas y Sociales Universidad de La Laguna38071 La LagunaSpain Instituto Universitario de Desarrollo Regional Universidad de La Laguna38071 La LagunaSpain

Isolated energy systems in archipelagos are characterized for having a great dependence on fossil sources due to isolation and territorial *** island of La palma is situated on the northwest of the Canary Islands,and its electric system is very *** policies planned by local authorities are aimed to increase the share of renewable energies and the reduction of fossil ***,intermittence and the concentration of unmanageable renewable energies in few locations may hinder the operation of the *** order to solve these problems,energy storage plays an essential *** aim of this paper is to analyse the effects of the introduction of two possible alternatives as a way of energy storage:pumped hydro storage and electric *** this,we use a simulation model adapted to the features of La palma,considering different *** show that,in the most favourable scenario,the installation of an additional 25 MW from renewables(more than double the current power),supported by 20 MW of pumped hydro storage and a fleet of 3361 electric vehicles,would allow the current share of renewables to increase from11%(in 2015)to 49%.Furthermore,this would mean a 26%reduction in CO2 emissions,10%in costs of generated k Wh and 19%in energy dependence.

关键词： Energy storage Electric vehicles Vehicle-to-grid Grid-for-vehicles Pumped-hydro energy storage Isolated systems

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Detection of β-Glucuronidase Activity within Actinomadura madurae Grains of Human Actinomycetoma

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Advances in Microbiology 2014年第6期4卷 317-323页

作者： alejandro palma-ramos Samantha Reyes-Mayén Laura Estela Castrillón-Rivera Silvia Elena Fernández-López Carmen Padilla-Desgarennes María Elisa Vega-Memije Roberto Arenas-Guzmán María Elisa Drago-Serrano Teresita Sainz-Espunes Department of Biological Systems Universidad Autónoma Metropolitana-XochimilcoMexico CityMéxico Dermatology Center“Dr.Ladislao de la Pascua” Mycology ServiceMexico City Health SecretaryMexico CityMéxico General Hospital“Manuel Gea González” Dermatology ServiceMexico City Health SecretaryMexico CityMéxico General Hospital“Manuel Gea González” Mycology ServiceMexico City Health SecretaryMexico CityMéxico

Actinomycetoma syndrome by Actinomadura (A.) madurae is characterized by a subcutaneous chronic lesion that affects fascia, muscle and bone. A. madurae produces colonies that form grains of less than 1 mm in diameter. Grains are surrounded and infiltrated by neutrophils involved in the grain disruption by enzymes like β-glucuronidase released after the neutrophil degranulation. The aim of this work was to evaluate the polysaccharide degradation of grains treated with β-glucuronidase and to detect the presence and activity of β-glucuronidase within the A. madurae grains. Actinomadura madura grains from patients infected were processed to quantify the total content of polysaccharide with the phenol-sulfuric acid reaction. Grains were treated with β-glucuronidase at different conditions to evaluate the optimal polysaccharide degradation. Grains were analyzed to detect the enzyme by using anti-human β-glucuronidase antibody while enzymatic activity was assessed by evaluating the release of reduced sugars and by in situ enzymatic activity. Optimal degradation of polysaccharide in the grains treated with β-glucuronidase was found with 300 units/ml of enzyme and 24 hr of incubation at 37°C. Presence and activity of β-glucuronidase enzyme within the grains were detected. Results suggested that β-glucuronidase present within A. madurae grain resulted from degranulated neutrophils surrounding and/or infiltrated within the grain.

关键词： Actinomycetoma A. madurae β-Glucuronidase

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Effect of Pregabalin and Gabapentin on Nociceptive Behaviors Induced by Spinal Nerve Ligation

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Journal of Biosciences and Medicines 2022年第3期10卷 114-123页

作者： Itzel I. ramos-Rodríguez Crystell G. Guzmán Priego Erick J. Rodríguez-palma Guadalupe del C. Baeza-Flores Jorge E. Torres-López Pain Mechanisms Laboratory Divisi&oacute n Acadé mica de Ciencias de la Salud Universidad Ju&aacute rez Autó noma de Tabasco Villahermosa Mexico Neurobiology of Pain Laboratory Departamento de Farmacobiología Neurobiology of Pain Departamento de Farmacobiología Cinvestav Sede Sur México City México

Pain is defined as an unpleasant sensory and emotional experience, associated with actual or potential tissue damage. According to its neurobiological mechanism, pain is classified into nociceptive, inflammatory, dysfunctional, and neuropathic. Neuropathic pain (NP) is caused by a lesion or disease of the somatosensory nervous system. Both pregabalin and gabapentin are pharmaceuticals used as validation drugs in experimental models of NP. Pregabalin was shown to produce significant antihyperalgesic and antiallodynic effects. Gabapentin is used as a reference compound for new analgesics and reduces tactile allodynia in rats. The aim of this work is to evaluate pregabalin and gabapentin effects on nociceptive behaviors induced by spinal nerve ligation (SNL). Female Wistar rats of 140 - 160 g were used, divided into five groups: Naive, SHAM, SNL rats treated with saline solution, SNL rats treated with pregabalin 30 mg/kg p.o., SNL rats treated with gabapentin 300 mg/kg p.o. Nociceptive behaviors were determined by the up and down method. In the establishment of SNL-induced allodynic behavior, a reduction in paw withdrawal threshold was observed in the time course, which was present from day 1 and it was maintained for 28 days post-ligation. With the administration of pregabalin and gabapentin, anti-allodynic behavior was observed in the time course and in the areas under the curve (AUC) of the time course of anti-allodynic behavior, significant difference was observed between pregabalin, and gabapentin groups compared to vehicle with a value of p < 0.0001. The results showed pregabalin and gabapentin induce an antinociceptive effect in rats subjected to SNL.

关键词： Tactile Allodynia Neuropathic Pain Pregabalin Gabapentin

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Elevated soluble fas blood concentrations in patients dying from spontaneous intracerebral hemorrhage

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World Journal of Critical Care Medicine 2023年第2期12卷 63-70页

作者： Leonardo Lorente María M Martín Antonia Pérez-Cejas Luis ramos-Gómez Jordi Solé-Violan Juan J Cáceres alejandro Jiménez Agustín F González-Rivero Department of Intensive Care Hospital Universitario de CanariasLa Laguna 38320TenerifeSpain Department of Intensive Care Hospital Universitario Nuestra Señora de CandelariaSanta Cruz de Tenerife 38010TenerifeSpain Department of Laboratory Hospital Universitario de CanariasLa Laguna 38320TenerifeSpain Department of Intensive Care Hospital Universitario de La PalmaBreña Alta 38713Spain Department of Critical Care Hospital Universitario de Gran Canaria Dr NegrinLas Palmas de Gran Canaria 35010Spain Department of Critical Care Universidad Fernando Pessoa CanariasLas Palmas de Gran Canaria 35450Spain Department of Intensive Care Hospital InsularLas Palmas de Gran Canaria 35016Spain Research Unit Hospital Universitario de CanariasLa Laguna 38320TenerifeSpain

BACKGROUND Several studies of spontaneous intracerebral hemorrhage(SICH)patients have shown apoptotic changes in brain samples after hematoma ***,there have been no data on the association between blood concentrations of soluble fas(sFas)(the main surface death receptor of the extrinsic apoptosis pathway)and the prognosis of spontaneous intracranial hypotension(SIH)*** To determine whether there is an association between blood sFas concentrations and SICH patient *** We included patients with severe and supratentorial *** was defined as having Glasgow Coma Scale<*** determined serum sFas concentrations at the time of severe SICH *** We found that non-surviving patients(n=36)compared to surviving patients(n=39)had higher ICH score(P=0.001),higher midline shift(P=0.004),higher serum sFas concentrations(P<0.001),and lower rate of early hematoma evacuation(P=0.04).Multiple logistic regression analysis showed an association between serum sFas concentrations and 30-d mortality(odds ratio=1.070;95%confidence interval=1.014-1.129;P=0.01)controlling for ICH score,midline shift,and early hematoma *** The association of blood sFas concentrations and SICH patient mortality is a novel finding in our study.

关键词： Spontaneous intracerebral hemorrhage Soluble fas Apoptosis Patients Mortality

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Activation and IL-1B secretion of human peripheral phagocytes infected with Actinomadura madurae,Nocardia asteroides and Candida albicans

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Asian Pacific Journal of Tropical Medicine 2016年第10期9卷 939-944页

作者： alejandro palma-ramos Gilberto Casillas-Petriz Laura Estela Castrillon-Rivera Jorge Ismael Castaneda-Sanchez Roberto Arenas-Guzman Maria Elisa Drago-Serrano Teresita Sainz-Espunes Department of Biological Systems Autonomous Metropolitan University Campus XochimilcoCalzada del Hueso No.1100Ciudad de Mexico 04960Mexico Service of Dermatology and Mycology General Hospital ‘Manuel Gea Gonzalez’Calzada de Tlalpan 4800TlalpanSeceion XVI14080 Ciudad de MexicoMexico

Objective: To evaluate the ability of Actinomadura madurae(A. madurae) and Nocardia asteroids(N. asteroides), using Candida albicans(C. albicans) as prototypic control, to elicit the activation and IL-1β secretion of blood phagocytic cells from healthy donors. Methods: Microcopic evaluation of phagocytosis/activation, cell viability and spectrophotometric quantitation of endocytosis/activation, were assessed by using formazan blue test in human blood phagocytes infected with C. albicans, A. madurae or N. asteroides treated with either normal human serum(NHS) or with decomplemented NHS. Interlukin-1β from culture supernatants of infected polymorphonuclear was tested by ELISA kit assay. Results: Microscopic assay showed that phagocytosis and activation of adherent mononuclear phagocytes were greater with C. albicans followed by A. madurae and then by N. asteroides. Spectrophotometric assay in polymornuclear phagocytes infected with NHS-treated pathogens indicated that activation was similarly higher by C. albicans and A. madurae and lower by N. asteroides. Kinetic assays in infected polymorphonuclear cells showed that viability was decreased by C. albicans and N. asteroides or unaffected with A. madurae. Levels of IL-1β at 8 h of incubation were higher with C. albicans followed by A. madurae whereas lower levels were found with N. asteroides. Conclusions: The extent of cell-viability and activation as well IL-1β secretion may be related with the virulence of C. albicans and N. asteroides and other parameters remain to be explored for assesing the virulence of A. madurae.

关键词： Interleukin-1B Phagocytosis Actinomycetoma Formazan blue assay

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