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Diet and monensin influence the temporal dynamics of the rumen microbiome in stocker and finishing cattle

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Journal of Animal Science and biotechnology 2024年第2期15卷 733-748页

作者： Jianmin Chai Caleb P.Weiss Paul A.beck Wei Zhao Ying Li Jiangchao Zhao Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise breeding College of Life Science and EngineeringFoshan UniversityFoshanChina Division of Agriculture Department of Animal ScienceUniversity of ArkansasFayettevilleARUSA Department of Animal and Food Sciences Oklahoma State UniversityStillwaterOKUSA Institute of Feed Research of Chinese Academy of Agricultural Sciences Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural AffairsBeijing 100193China

background Stocker cattle diet and management influence beef cattle performance during the finishing stage,but knowledge of the dynamics of the rumen microbiome associated with the host are lacking.A longitudinal study was conducted to determine how the feeding strategy from the stocker to the finishing stages of production affects the temporal dynamics of rumen *** the stocker phase,either dry hay or wheat pasture were provided,and three levels of monensin were *** calves were then transported to a feedlot and received similar finishing diets with or without *** microbial samples were collected on d 0,28,85 during the stocker stage(S0,S28 and S85)and d 0,14,28,56,30 d before slaughter and the end of the trial during the finishing stage(F0,F14,F28,F56,Pre-ba,and Final).The V4 region of the bacterial 16S rRNA gene of 263 rumen samples was *** Higher alpha diversity,including the number of observed bacterial features and the Shannon index,was observed in the stocker phase compared to the finishing *** bacterial amplicon sequence variants(ASVs)differentiating different sampling time points were *** treatments during the stocker stage temporally impact the dynamics of rumen *** example,shared bacteria,including bacteroidales(ASV19)and Streptococcus infantarius(ASV94),were significantly higher in hay rumen on S28,S85,and F0,while bacteroidaceae(ASV11)and Limivicinus(ASV15)were more abundant in *** affected rumen microbial composition at a specific *** to feedlot significantly influenced microbiome structure and diversity in hay-fed *** taxa associated with body weight were classified,and core microbiotas interacted with each other during the *** In summary,the temporal dynamics of the rumen microbiome in cattle at the stocker and finishing stage are influenced by multiple factors of the feeding *** at the stocker phase may t

关键词： beef cattle Diet Feedlot phase Monensin Next-generation sequencing Rumen microbiota Stocker

Matched pair analysis of the effect of longer hypothermic machine perfusion time on kidney transplant outcomes

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World Journal of Transplantation 2024年第3期14卷 95-101页

作者： Carlos Verdiales Luke baxter Hyun Ja Lim Gavin beck Michael A Moser Department of Surgery University of SaskatchewanSaskatoonSaskatchewan S7N 0W8Canada College of Medicine University of SaskatchewanSaskatoonSaskatchewan S7N 0W8Canada Canada and Northern Ontario School of Medicine Thunder BayOntario P7B 5E1Canada Department of Community Health and Epidemiology University of SaskatchewanSaskatoonSaskatchewan S7N 0W8Canada

bACKGROUND Hypothermic machine perfusion(HMP)has demonstrated benefits in terms of early kidney transplant function compared to static cold *** longer preservation times have shown detrimental effects,a previous paired study indicated that longer pump times(the second kidney in a pair)might lead to improved *** To revisit the prior paired study's somewhat unexpected results by reviewing our program's *** A total of 61 pairs of transplant recipients who received kidneys from the same donor(2012-2021)were *** were divided into two groups depending on whether they were transplanted first(K1)or second(K2).Therefore,the patients in each pair had identical donor characteristics,except for time on the *** analyses included Kaplan-Meyer analysis and paired tests,including McNemar's test,student's paired t-test,or Wilcoxon's test,as *** The two groups of recipients had similar demographics(age,body mass index,diabetes,time on dialysis,sensit-ization and retransplants).Cold ischemic times for K1 and K2 were 8.9(95%CI:7.9,9.8)and 14.7 hours(13.7,15.8)(Pbiopsy-proven acute rejection at 1 year(P=0.015).Delayed graft function was less common in K2,12/61(20%)than in K1,20/61(33%)(P=0.046).Finally,K2 showed a higher graft survival than K1(P=0.023).CONCLUSION Our results agree with a previous study that suggested possible advantages to longer pump *** studies should encourage further research into HMP's potential anti-inflammatory effect.

关键词： Hypothermic machine perfusion Paired kidney study Early acute rejection

Hepatitis b virus replication

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World Journal of Gastroenterology 2007年第1期13卷 48-64页

作者： Juergen beck Michael Nassal Department of Internal Medicine II/Molecular biology University Hospital FreiburgGermany

Hepadnaviruses, including human hepatitis b virus （HbV）, replicate through reverse transcription of an RNA intermediate, the pregenomic RNA （pgRNA）. Despite this kinship to retroviruses, there are fundamental differences beyond the fact that hepadnavirions contain DNA instead of RNA. Most peculiar is the initiation of reverse transcription： it occurs by protein-priming, is strictly committed to using an RNA hairpin on the pgRNA, ε, as template, and depends on cellular chaperones; moreover, proper replication can apparently occur only in the specialized environment of intact nucleocapsids. This complexity has hampered an in-depth mechanistic understanding. The recent successful reconstitution in the test tube of active replication initiation complexes from purified components, for duck HbV （DHbV）, now allows for the analysis of the biochemistry of hepadnaviral replication at the molecular level. Here we review the current state of knowledge at all steps of the hepadnaviral genome replication cycle, with emphasis on new insights that turned up by the use of such cellfree systems. At this time, they can, unfortunately, not be complemented by three-dimensional structural information on the involved components. However, at least for the ~ RNA element such information is emerging, raising expectations that combining biophysics with biochemistry and genetics will soon provide a powerful integrated approach for solving the many outstanding questions. The ultimate, though most challenging goal, will be to visualize the hepadnaviral reverse transcriptase in the act of synthesizing DNA, which will also have strong implications for drug development.

关键词： Chaperone-mediated reverse transcription HbV cccDNA Hepadnavirus, P protein Pregenomic RNA Protein-priming reverse transcriptase RNA encapsidation signal

Rapid Genomic Evolution of a Non-virulent Coxsackievirus b3 in Selenium-deficient Mice

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biomedical and Environmental Sciences 1997年第2期10卷 307-315页

作者： MELINDA A.beck University of North Carolina at Chapel Hill Frank Porter Graham Child Development Center105 Smith Level RdCB#8180Chapel HillNC 27599-8180USA

Keshan disease, an endemic cardiomyopathy in China, can be prevented with selenium (Se) supplementation. However, the seasonal and annual nature of the disease suggests that an infectious co-factor is required along with a deficiency in Se. Using a murine model of coxsackievirus b3 (CVb3) -induced myocarditis, Se-deficient mice were shown to be more susceptible to the cardiopathologic effects of the virus. In addition, a normall benign strain of CVb3 becomes virulent in Se-deficient mice. This change in virulence was shown to be due to point mutations in the viral genorne. Although the mechanism of the viral mutation is not known, the oxidative stress status of the Se-deficient host may play a role, either by directly affecting the virus and/or affecting host immune defenses

关键词： CVb

Somatic Mutations in UbA1 and Severe Adult-Onset Autoinflammatory Disease

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四川生理科学杂志 2020年第4期42卷 414-414页

作者： David b beck From the National Human Genome Research Institute (D.b.b. A.K.O. W.P. N.B. D.L.R. D.O.C. K.M. P.H. S.R. L.X. H.O. M.N. A.J. R.S.L. N.D. J.J.C. M.C.V.M. D.N. B.D.S. W.A.G. S.M.B. I.A. D.L.K.) the National Institute of Arthritis and Musculoskeletal and Skin Diseases (M.A.F. K.A.S. S.N. W.L.T. S.G. C.C.-R. W.G. E.R. K.V.W. G.W. S.B. S.D. Z.D. R.A.C. M.J.K. M.G. P.C.G.) the National Institute of Dental and Craniofacial Research (J.C.C. A.J.A. A.W.) the Undiagnosed Diseases Program Common Fund Office of the Director (N.B. D.L.R. M.C.V.M. D.N. W.A.G.) the Hematology Branch National Heart Lung and Blood Institute (Z.W. B.P. E.M.G. F.G.-R. L.W.D. C.S.H. N.S.Y.) the National Institute of Allergy and Infectious Diseases (K.S.B.) the Laboratory of Pathology Center for Cancer Research National Cancer Institute (C.-C.R.L.) the National Institutes of Health (NIH) Intramural Sequencing Center National Human Genome Research Institute (J.M.) the Department of Laboratory Medicine (W.W. M.T. A.D.-F. K.R.C.) and the National Eye Institute (M.A.-A.) NIH Bethesda and GeneDx Gaithersburg (K.R.) — both in Maryland and the National Amyloidosis Centre Royal Free Hospital London NHS Foundation Trust and University College London London (D.R. H.J.L.) and the National Institute for Health Research–Leeds Biomedical Research Centre and Leeds Institute of Rheumatic and Musculoskeletal Medicine University of Leeds Leeds (S.S.) — both in the United Kingdom.

background:Adult-onset inflammatory syndromes often manifest with overlapping clinical *** in ubiquitin-related genes,previously implicated in autoinflammatory disease,may define new ***:We analyzed peripheral-blood exome sequence data independent of clinical phenotype and inheritance pattern to identify deleterious mutations in ubiquitin-related *** sequencing,immunoblotting,immunohistochemical testing,flow cytometry,and transcriptome and cytokine profiling were ***-Cas9-edited zebrafish were used as an in vivo model to assess gene function.

关键词： inflammatory clinical Adult

Agriculture Resource Management Planning on the Wind River Indian Reservation: An Integrative Approach

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Journal of Geoscience and Environment Protection 2019年第12期7卷 206-221页

作者： Ginger b. paige William J. Gribb Roger H. Coupal Department of Ecosystem Science and Management College of Agriculture and Natural Resources University of Wyoming Laramie WY USA Department of Geography College of Arts and Sciences University of Wyoming Laramie WY USA Department of Agricultural and Applied Economics College of Agriculture and Natural Resources University of Wyoming Laramie WY USA

The Wind River Indian Reservation undertook the development of an agricultural resource management plan in 2015. The reservation is 8903 *** of which 63% is in crop and grazing lands. This project’s aim was to create a community-based integrated plan. A series of community meetings identified 83 issues and topics relating to agricultural development on the reservation. Using information from community meetings, the final agricultural resource management plan consists of 9 major elements, with 12 goals, 46 objectives and 88 policies. Three key issues are addressed in the plan: provide the reservation with a plan that leads to self-management of their agricultural resources;integrate the major agricultural elements as defined by the community into policies;and, create a governance structure that fosters agricultural development into the future.

关键词： Agricultural Planning Integrative Wind River Indian Reservation Community-based

Adjusting the physico-chemical properties of collagen scaffolds to accommodate primary osteoblasts and endothelial cells

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Regenerative biomaterials 2023年第1期10卷 590-602页

作者： Nima Meyer Daniel V.bax Jochen beck Ruth E.Cameron Serena M.best Department of Materials Science and Metallurgy University of CambridgeCambridge CB30FSUK Geistlich Pharma AG Product DevelopmentWolhusenSwitzerland

Collagen-based biomaterials are used widely as tissue engineering scaffolds because of their excellent bioactivity and their similarity to the natural *** regeneration of healthy bone tissue requires simultaneous support for both osteoblasts and,where angiogenesis is intended,endothelial *** it is important to tailor carefully the biochemical and structural characteristics of the scaffold to suit the needs of each cell *** work describes for the first time a systematic study to gain insight into the cell type-specific response of primary human osteoblast(hObs)and human dermal microvascular endothelial cells(HDMECs)to insoluble collagen-based *** behaviour was evaluated on both 2D films and 3D scaffolds,produced using *** collagen was cross-linked at various EDC/NHS concentrations and mono-cultured with hObs and HDMECs to assess the effect of architectural features and scaffold stabilization on cell *** was observed that 3D scaffolds cross-linked at 30%of the standard conditions in literature offered an optimal combination of mechanical stiffness and cellular response for both cell types,although endothelial cells were more sensitive to the degree of cross-linking than *** features have a time-dependent impact on the cell migration profile,with alignment being the most influential parameter overall.

关键词： angiogenesis collagen scaffolds cell migration EDC/NHS cross-linking

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特种光纤的研制进展

光电子技术与信息 1993年第3期6卷 25-27页

作者： beck,b 南山

Microsoft Visual C++使用指南

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