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Generation of diverse neural cell types through direct conversion

World Journal of Stem Cells 2016年第2期8卷 32-46页

作者： Gayle F Petersen padraig m strappe School of Biomedical Sciences Charles Sturt University

A characteristic of neurological disorders is the loss of critical populations of cells that the body is unable to replace,thus there has been much interest in identifying methods of generating clinically relevant numbers of cells to replace those that have been damaged or *** process of neural direct conversion,in which cells of one lineage are converted into cells of a neural lineage without first inducing pluripotency,shows great potential,with evidence of the generation of a range of functional neural cell types both in vitro and in vivo,through viral and non-viral delivery of exogenous factors,as well as chemical induction *** neural cells have been proposed as an attractive alternative to neural cells derived from embryonic or induced pluripotent stem cells,with prospective roles in the investigation of neurological disorders,including neurodegenerative disease modelling,drug screening,and cellular replacement for regenerative medicine applications,however further investigations into improving the efficacy and safety of these methods need to be performed before neural direct conversion becomes a clinically viable *** this review,we describe the generation of diverse neural cell types via direct conversion of somatic cells,with comparison against stem cell-based approaches,as well as discussion of their potential research and clinical applications.

关键词： Adult stem cells Embryonic stem cells Generation of neural cells Induced pluripotent stem cells In vitro differentiation In vivo differentiation Clinical applications Direct conversion Induced neural cells

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Parallel mRNA, proteomics and miRNA expression analysis in cell line models of the intestine

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World Journal of Gastroenterology 2017年第41期23卷 7369-7386页

作者： Finbarr O’Sullivan Joanne Keenan Sinead Aherne Fiona O’Neill Colin Clarke michael Henry Paula meleady Laura Breen Niall Barron martin Clynes Karina Horgan padraig Doolan Richard murphy National Institute for Cellular Biotechnology Dublin City University National Institute for Bioprocessing Research & Training Alltech Dunboyne

AIm To identify mi RNA-regulated proteins differentially expressed between Caco2 and HT-29: two principal cell line models of the *** Exponentially growing Caco-2 and HT-29 cells were harvested and prepared for m RNA, mi RNA and proteomic profiling. m RNA microarray profiling analysis was carried out using the Affymetrix Gene Chip Human Gene 1.0 ST array. mi RNA microarray profiling analysis was carried out using the Affymetrix Genechip mi RNA 3.0 array. Quantitative Label-free LC-mS/mS proteomic analysis was performed using a Dionex Ultimate 3000 RSLCnano system coupled to a hybrid linear iontrap/Orbitrap mass spectrometer. Peptide identities were validated in Proteome Discoverer 2.1 and were subsequently imported into Progenesis QI software for further analysis. Hierarchical cluster analysis for all three parallel datasets(mi RNA, proteomics, m RNA) was conducted in the R software environment using the Euclidean distance measure and Ward's clustering algorithm. The prediction of mi RNA and oppositely correlated protein/m RNA interactions was performed using Target Scan 6.1. GO biological process, molecular function and cellular component enrichment analysis was carried out for the DE mi RNA, protein and m RNA lists via the Pathway Studio 11.3 Web interface using their mammalian *** Differential expression(DE) profiling comparing the intestinal cell lines HT-29 and Caco-2 identified 1795 Genes, 168 Proteins and 160 mi RNAs as DE between the two cell lines. At the gene level, 1084 genes were upregulated and 711 were downregulated in the Caco-2 cell line relative to the HT-29 cell line. At the protein level, 57 proteins were found to be upregulated and 111 downregulated in the Caco-2 cell line relative to the HT-29 cell line. Finally, at the mi RNAs level, 104 were upregulated and 56 downregulated in the Caco-2 cell line relative to the HT-29 cell line. Gene ontology(GO) analysis of the DE m RNA identified cell adhesion, migration and ECm o

关键词： Caco-2 HT-29 microarray Proteomics miRNA Targetscan Gene ontology

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