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Punch‑excised explants of bovine mammary gland to model early immune response to infection

Journal of Animal Science and Biotechnology 2023年第6期14卷 2649-2662页

作者： pablo gomes noleto Florence B.Gilbert Christelle Rossignol Patricia Cunha Pierre Germon Pascal Rainard Rodrigo Prado Martins ISP INRAEUniversitéde ToursUMR1282NouzillyFrance

Background Mammary gland(MG)infections(mastitis)are frequent diseases of dairy cows that affect milk quality,animal welfare and farming *** infections are commonly associated with the bacteria Escherichia coli and Staphylococcus *** in vitro models have been used to investigate the early response of the MG to bacteria,but the role of the teat in mastitis pathogenesis has received less *** this study,we used punch-excised teat tissue as an ex vivo model to study the immune mechanisms that arise early during infection when bacteria have entered the *** Cytotoxicity and microscopic analyses showed that bovine teat sinus explants have their morphology and viability preserved after 24 h of culture and respond to ex vivo stimulation with TLR-agonists and *** and *** trigger stronger inflammatory response in teat when compared to LTA and ***,leading to a higher produc-tion of IL-6 and IL-8,as well as to an up-regulation of proinflammatory *** also demonstrated that our ex vivo model can be applied to frozen-stored *** In compliance with the 3Rs principle(replacement,reduction and refinement)in animal experimenta-tion,ex vivo explant analyses proved to be a simple and affordable approach to study MG immune response to *** model,which better reproduces organ complexity than epithelial cell cultures or tissue slices,lends itself particularly well to studying the early phases of the MG immune response to infection.

关键词： Bovine teat Escherichia coli Explant Mastitis Staphylococcus aureus 3Rs

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Cardiovascular risk burden and disability:findings from the International Mobility in Aging Study(IMIAS)

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Journal of Geriatric Cardiology 2024年第3期21卷 331-339页

作者： Juan-David Martinez-Aristizábal Carmen-Lucia Curcio Juliana Fernandes Afshin Vafael Cristiano dos Santos gomes Fernando Gomez Internal Medicine Critical Care DepartmentPablo Tobón Uribe HospitalMedellínColombia Research Group in Geriatrics and Gerontology Faculty of Health SciencesUniversidad de CaldasManizalesColombia Laboratory of Physical Therapy and Collective Health Physical Therapy DepartmentFederal University of PernambucoJornalista Aníbal Fernandes Avenue RecifeBrazil Department of Public Health Sciences Queen’s UniversityKingstonONCanada Department of Physiotherapy University of PernambucoBR 203KmBrazil

BACKGROUND The association of cardiovascular risk burden with disability is *** examined the association between trajectories of the Framingham general cardiovascular disease risk score(FGCRS)with the trajectories of limitations of physical function in older *** A total of 1219 participants with no disabilities from the International Mobility in Aging Study(IMIAS)study who had up to three repeated measures of FGCRS between 2012–2016 and without a history of stroke or coronary heart disease at baseline and follow-up were *** at baseline was assessed and categorized into *** function was evaluated with the Short Physical Performance Battery(SPPB).The data were analyzed using linear mixed-effects *** At baseline,FGCRS ranged between 3–94(mean score:24±15.8),participants were 32(2.6%),502(41.2%)and 685(56.2%)in lowest,middle,and highest tertiles,*** the trajectories of limitations of physical function,the lowest FGCRS had no differences,while the middle and highest had a decrease in physical performance between 2012–2014(P=0.0001).Age,being female,living in Andes Mountains,having middle and highest FGCRS,higher alcohol consumption,being obese,lack of exercise and cognitive impairment increase the probability of disability(P<0.05).Alternatively,living in more developed regions and having a higher educational level reduced the probability of disability during the follow-up time(P<0.05).CONCLUSIONS Higher cardiovascular risk burden is associated with decreased physical performance,especially in *** suggest SPPB may provide a measure of cardiovascular health in older adults.

关键词： finding Battery burden

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