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面向撞击载荷的疲劳寿命分析及验证

机电元件 2024年第1期44卷 39-44页

作者：赵正阳张楚贤冯萃峰蔡立兵刘文成王子文北京航天微机电技术研究所北京100094

针对宇航用波导开关在反复切换撞击工况下的寿命指标要求,开展疲劳寿命设计与验证。在实际工况应力谱难以获得的情况下,基于数值计算方法,采用两种不同方法获取应力谱后开展疲劳寿命分析并进行了试验验证,验证结果表明:基于瞬态动力学... 详细信息

针对宇航用波导开关在反复切换撞击工况下的寿命指标要求,开展疲劳寿命设计与验证。在实际工况应力谱难以获得的情况下,基于数值计算方法,采用两种不同方法获取应力谱后开展疲劳寿命分析并进行了试验验证,验证结果表明:基于瞬态动力学的疲劳分析有较高的准确性,在此基础上,优化改进产品结构,使产品切换寿命满足指标要求。

关键词：波导开关疲劳寿命应力谱瞬态动力学

cDNA CLONING OF RAT 2B1 ANTIGEN;A SPERM SURFACE GLYCOPROTEIN IMPLICATED IN ZONA PELLUCIDA BINDING.

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cDNA CLONING OF RAT 2B1 ANTIGEN;A SPERM SURFACE GLYCOPROTEIN...

第二次北京生育调节国际学术研讨会

作者： Alicia Ma Roy Jones len Hall Babraham Institute CambridgeUK. Dept.of Biochemistry BristolUK University of Bristol

＜正＞The rat sperm surface 2B1 antigen has been proposed to play a key role in sperm adhesion to the zona *** have shown that rat 2B1 is synthesised in the testis as a precursor glycoprotein which subsequently undergoes processing to a lower molecular weight form within the epididymis. 2B1 antigen also actively migrates from its location on the tail to the acrosomal region during in vitro capacitation.

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数据模型资源手册卷1 修订版

A comparative analysis of entomoparasitic nematodes <i>Heterorhabditis bacteriophora</i>and <i>Steinernema carpocapsae</i>

丛书名：数据库技术丛书

2004年

作者：（美）len Silverston 林友芳等译

华章图书:本书介绍通用数据模型，涉及人与组织、产品定制、装运和接收、预算背景、雇员资格和表现等大量经过验证的数据模型，及新的数据集市设计内容。

ISBN: (纸本)7111141210

关键词：企业管理数据模型

来源：畅想之星图书光盘版（南... 畅想之星图书光盘版（南... 评论

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Open Journal of Animal Sciences 2013年第4期3卷 326-333页

作者： Rinu Kooliyottil Devang Upadhyay Floyd Inman III Sivanadane Mandjiny len Holmes Sartorius Stedim Biotechnology Laboratory Biotechnology Research and Training Center University of North Carolina at Pembroke Pembroke USA Sartorius Stedim Biotechnology Laboratory Biotechnology Research and Training Center University of North Carolina at Pembroke Pembroke USA

Heterorhabditis bacteriophora and Steinernema carpocapsae are microscopic entomoparasitic nematodes (EPNs) that are attractive, organic alternatives for controlling a wide range of crop insect pests. EPNs evolved with parasitic adaptations that enable them to “feast” upon insect hosts. The infective juvenile, a non-feeding, developmentally arrested nematode stage, is destined to seek out insect hosts and initiates parasitism. After an insect host is located, EPNs enter the insect body through natural openings or by cuticle penetration. Upon access to the insect hemolymph, bacterial symbionts (Photorhabdus luminescens for H. bacteriophora and Xenorhabdus nematophila for S. carpocapsae) are regurgitated from the nematode gut and rapidly proliferate. During population growth, bacterial symbionts secrete numerous toxins and degradative enzymes that exterminate and bioconvert the host insect. During development and reproduction, EPNs obtain their nutrition by feeding upon both the bioconverted host and proliferated symbiont. Throughout the EPN life cycle, similar characteristics are seen. In general, EPNs are analogous to each other by the fact that their life cycle consists of five stages of development. Furthermore, reproduction is much more complex and varies between genera and species. In other words, infective juveniles of S. carpocapsae are destined to become males and females, whereas H. bacteriophora develop into hermaphrodites that produce subsequent generations of males and females. Other differences include insect host range, population growth rates, specificity of bacterial phase variants, etc. This review attempts to compare EPNs, their bacterial counterparts and symbiotic relationships for further enhancement of mass producing EPNs in liquid media.

关键词： Entomoparasitic Nematodes (EPNs) Heterorhabditis Bacteriophora Steinernema Carpocapsae Photorhabdus Luminescens Xenorhabdus Nematophila Symbiosis Mass Production

张扬青春活力,就是Street Dance!

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青春期健康 2010年第11期8卷 60-61页

作者： len

街舞（Street Dance）最早起源于美国纽约，是爵士舞发展到上发展到上世纪90年代的产物，是美国黑人Hip-Hop文化的一种。

关键词：青春活力张扬美国黑人

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兼具凝集素活性的芥菜几丁质酶BjCHI1

生物工程学报 2002年第5期18卷 572-577页

作者：欧阳石文赵开军冯兰香 CHYE Mee-len RAM Sathishkvmar 中国农业科学院蔬菜花卉研究所北京100081 香港大学植物系

利用毕赤酵母表达系统表达芥菜几丁质酶基因BjCHI1及其两个衍生基因BjCHI2和BjCHI3,获得相应的蛋白质。经FPLC纯化后 ,测定了 3种蛋白质的几丁质酶活性 ,发现它们均能降解CM chitin RBV和胶状几丁质。以CM chitin RBV为底物时的Km值分别... 详细信息

利用毕赤酵母表达系统表达芥菜几丁质酶基因BjCHI1及其两个衍生基因BjCHI2和BjCHI3,获得相应的蛋白质。经FPLC纯化后 ,测定了 3种蛋白质的几丁质酶活性 ,发现它们均能降解CM chitin RBV和胶状几丁质。以CM chitin RBV为底物时的Km值分别为 0 .799mg mL、0 .5 44mg mL和 0 .793mg mL ,差别甚微。而以胶状几丁质为底物时的Km值分别为 0 .2 81mg mL、0 .388mg mL和 1.6 43mg mL ,表现一定的差别 ,说明几丁质结合域影响了酶对不溶性底物的亲和力。 3种蛋白中 ,只有BjCHI1在 33μg mL以上浓度具有凝集素活性 ,而BjCHI2和BjCHI3的浓度即使高达 80 0 μg mL也无凝集素活性 ,表明 2个几丁质结合域是BjCHI1具有凝集素活性的必需条件。

关键词：凝集素活性芥菜几丁质酶 BjCHI1

Polyunsaturated linolenoyl-Co A modulates ERF-Ⅶ mediated hypoxia signaling in Arabidopsis

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Journal of Integrative Plant Biology 2020年第3期62卷 330-348页

作者： Ying Zhou Wei-Juan Tan Li-Juan Xie Hua Qi Yi-Cong Yang Li-Ping Huang Yong-Xia Lai Yi-Fang Tan De-Mian Zhou Lu-Jun Yu Qin-Fang Chen Mee-len Chye Shi Xiao State Key Laboratory of Biocontrol Guangdong Provincial Key Laboratory of Plant ResourcesSchool of Life SciencesSun Yat-Sen UniversityGuangzhou 510275China School of Biological Sciences The University of Hong KongPokfulamHong Kong 999077China

In plants,submergence from flooding causes hypoxia,which impairs energy production and affects plant growth,productivity,and *** Arabidopsis,hypoxia induces nuclear localization of the group VII ethylene-responsive transcription factor RELATED TO AP2.12(RAP2.12),following its dissociation from the plasma membrane-anchored ACYL-COA BINDING PRO-TEIN1(ACBP1)and ***,we show that polyunsaturated linolenoyl-Co A(18:3-Co A)regulates RAP2.12release from the plasma *** caused a significant increase in 18:3-Co A,but a significant decrease in 18:0-,18:1-,and 18:2-Co *** of 18:3-Co A promoted nuclear accumulation of the green fluorescent protein(GFP)fusions RAP2.12-GFP,HYPOXIA-RESPONSIVE ERF1-GFP,and RAP2.3-GFP,and enhanced transcript levels of hypoxia-responsive *** with decreased ACBP1 and ACBP2(acbp1 ACBP2-RNAi,produced by ACBP2 RNA interference in the acbp1 mutant)had reduced tolerance to hypoxia and impaired 18:3-Co A-induced expression of hypoxia-related *** knockout mutants and overexpression lines of LONG-CHAIN ACYL-COA SYNTHASE2(LACS2)and FATTY ACID DE-SATURASE 3(FAD3),the acyl-Co A pool size and 18:3-Co A levels were closely related to ERF-VII-mediated signaling and hypoxia *** findings demonstrate that polyunsaturation of long-chain acyl-Co As functions as important mechanism in the regulation of plant hypoxia signaling,by modulating ACBP–ERF-VII dynamics.

关键词： Arabidopsis ERF impaired

CoBRA: Containerized Bioinformatics Workflow for Reproducible Ch IP/ATAC-seq Analysis

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Genomics, Proteomics & Bioinformatics 2021年第4期19卷 652-661页

作者： Xintao Qiu Avery S.Feit Ariel Feiglin Yingtian Xie Nikolas Kesten len Taing Joseph Perkins Shengqing Gu Yihao Li paloma Cejas Ningxuan Zhou Rinath Jeselsohn Myles Brown XShirley Liu Henry W.Long Center for Functional Cancer Epigenetics Dana-Farber Cancer InstituteBostonMA 02215USA Department of Medical Oncology Dana-Farber Cancer InstituteHarvard Medical SchoolBostonMA 02215USA Albert Einstein College of Medicine BronxNY 10461USA Department of Biomedical Informatics Harvard Medical SchoolBostonMA 02215USA Department of Data Sciences Dana Farber Cancer InstituteHarvard T.H.Chan School of Public HealthBostonMA 02215USA

Chromatin immunoprecipitation sequencing(Ch IP-seq)and the Assay for Transposase-Accessible Chromatin with high-throughput sequencing(ATAC-seq)have become essential technologies to effectively measure protein–DNA interactions and chromatin ***,there is a need for a scalable and reproducible pipeline that incorporates proper normalization between samples,correction of copy number variations,and integration of new downstream analysis *** we present Containerized Bioinformatics workflow for Reproducible Ch IP/ATAC-seq Analysis(Co BRA),a modularized computational workflow which quantifies Ch IP-seq and ATAC-seq peak regions and performs unsupervised and supervised *** BRA provides a comprehensive state-of-the-art Ch IP-seq and ATAC-seq analysis pipeline that can be used by scientists with limited computational *** enables researchers to gain rapid insight into protein–DNA interactions and chromatin accessibility through sample clustering,differential peak calling,motif enrichment,comparison of sites to a reference database,and pathway *** BRA is publicly available online at https://***/cfce/cobra.

关键词： ChIP-seq ATAC-seq Snakemake Docker Workflow

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了解模数转换器规格

世界电子元器件 2005年第10期 36-44页

作者： len Staller Silicon Laboratories Inc.

制造商在组件资料表(dota sheet)中定义模数转换器效能的方式,常常会令人混淆不清,刚接触模数转换器的工程师对此尤感困扰。本文将帮助工程师深入了解制造商的组件资料表中用来描述逐次逼近SAR模数转换器效能的各种常见规格。

关键词： SAR模数转换器规格逐次逼近制造商工程师组件