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Effect of Korean Magnolia obovata Extract on Platelet-Derived Growth Factor-Induced Vascular Smooth Muscle Cells

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Chinese Journal of Integrative Medicine 2020年第9期26卷 677-682页

作者： Hyunjhung Jhun Suji Baek Jinwoo Kim Kang-pa.lee Hun-Young park Won-Hwan park Kiwon lim Jisu Kim Technical Assistance Center Korea Food Research InstituteJeonbuk(55365)Republic of Korea Department of Medical Science School of MedicineKonkuk UniversitySeoulRepublic of Korea Department of Bio-Science College of Natural ScienceDongguk UniversityGyeongbuk(38066)Republic of Korea Physical Activity&Performance Institute Konkuk UniversitySeoul(05029)Republic of Korea Department of Diagnostic College of Korean MedicineDongguk University GoyangGyeonggi-do(10326)Republic of Korea

Objective:To investigate the effects of Korean Magnolia obovata crude extract(KME)on plateletderived growth factor(PDGF)-BB-induced proliferation and migration of vascular smooth muscle cells(VSMCs).Methods:KME composition was analyzed by high-performance liquid chromatography(HPlC).VSMCs were isolated from the aorta of a Sprague-Dawley rat,incubated in serum free-Dulbecco's modified Eagle's medium in the presence or absence of KME(10,30,100,and 300(xg/ml),then further treated with PDGF-BB(10 ng/ml).VSMC proliferation was detected using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and VSMC migration was determined using the Boyden chamber and scratch wound healing *** blot analysis was used to detect phosphorylation of extracellular signal-regulated protein kinases 1 and 2(p-ERK1/2),protein kinase B(p-Akt),and stress-activated protein kinase/c-Jun NH2-terminal kinase(p-SAPK/JNK).The antimigration and proliferation effects of KME were tested using aortic sprout ***:The HPlC analysis identified honokiol(0.45 mg/g)and magnolol(0.34 mg/g)as the major components of ***(30,100,and 300μg/m l)significantly decreased the proliferation and migration of PDGF-BB-stimulated(10 ng/ml)VSMCs and the PDGF-BB-induced phosphorylation of EKR1/2,Akt,and SAPK/JNK(Pl of KME showed reduction in aortic sprout ***:KME could inhibit abnormal proliferation and migration of VSMCs by down-regulating the phosphorylation of EKR1/2 and ***,KME might be a functional food for preventing vascular disorders.

关键词： extracellular signal-regulated protein kinases 1 and 2 Akt platelet-derived growth factor-BB smooth muscle cell functional food

Resilience, the 6th Vital Sign: Conceptualizing, Contextualizing, and Operationalizing All Six Vital Signs

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Health 2024年第7期16卷 657-673页

作者： Rose E. Constantino Betty J. Braxter Chi Ching Vivian Hui larissa C. Allen lillian J. Wolfe Katherine H. Endres Jezyl Cempron Cutamora laurence l. Garcia Daisy R. palompon Kathleen Thimsen Brayden N. Kameg Margarete l. Zalon Department of Health and Community Systems School of Nursing University of Pittsburgh Pittsburgh PA USA Undergraduate Affairs School of Nursing University of Pittsburgh Pittsburgh PA USA The Hong Kong Polytechnic University Hong Kong China School of Nursing Adjunct Faculty at the University of Pittsburgh Pittsburgh PA USA School of Nursing University of Pittsburgh Pittsburgh PA USA Department of Health Promotion and Development School of Nursing University of Pittsburgh Pittsburgh PA USA College of Nursing Cebu Normal University Cebu City Philippines School of Nursing University of Nevada Las Vegas USA Department of Health and Community Systems University of Pittsburgh PA USA Department of Nursing University of Scranton Scranton PA USA

There are five vital signs that healthcare providers assess: temperature, pulse, respiration, blood pressure, and pain. Normal levels for the five vital signs are published by the American Heart Association, and other specialty organizations, however, the sixth vital sign (resilience) which adopts the measure of immune resilience is suggested in this paper. Resilience is the ability of the immune system to respond to attacks and defend effectively against infections and inflammatory stressors, and psychological resilience is the capacity to resist, adapt, recover, thrive, and grow from a challenge or a stressor. Individuals with better optimal immune resilience had better health outcomes than those with minimal immune resilience. The purpose of this paper is to conceptualize, contextualize, and operationalize all six vital signs. We suggest measuring resilience subjectively and objectively. Subjectively, use a 5-item guided interview revised from the Connor-Davidson Resilience Scale (CDRC), a scale of 10 items. The revised CDRC scale is a 5-item scale. The scale is rated on a 5-point likert scale from 0 (not true) to 4 (true all the time). The total score ranges from 0 to 20, with higher total scores indicating greater resilience. The scale demonstrated good construct validity and internal consistency (α = 0.85) during the development of the scale. The CD-RISC had a good Cronbach’s alpha level of 0.85. The Revised CD-RISC can be completed in 2 - 4 minutes. To measure resilience objectively, we suggest using Immune Resilience (IR) levels, the level of resilience to preserve and/or rapidly restore immune resilience functions that promote disease resistance and control inflammation and other inflammatory stress. IR levels are gauged with two peripheral blood metrics that quantify the balance between CD8 and CD4 T-cell levels and gene expression signatures tracking longevity-associated immunocompetence and mortality- or entropy-associated inflammation. IR deregulation i

关键词： Conceptualization Contextualization Operationalization Body Temperature Pulse Respiration Blood Pressure pain Resilience

Comprehensive profiling of CTP-binding proteins using a biotinylated CTP affinity probe

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Chinese Chemical letters 2021年第11期32卷 3479-3482页

作者： Mengting pa Yunming liu Xiaofang Zheng Meijuan Zhou Changjun You Xiaoxia Dai State Key laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringMolecular Science and Biomedicine LaboratoryHunan Provincial Key Laboratory of Biomacromolecular Chemical BiologyHunan UniversityChangsha 410082China

Recent studies have shown that CTP may act as a ligand to regulate the activity of its target proteins in many biological ***,proteome-wide identification of CTP-binding proteins remains ***,we employed a biotinylated CTP affinity probe coupled with stable isotope labeling by amino acids in cell culture(SIlAC)-based quantitative proteomics approach to capture,identify and quantify CTP-binding proteins in human *** performing two types of competitive SIlAC experiments with high *** concentrations of CTP probe(100 vs.10µmol/l)or with CTP probe in the presence of free CTP,we identified 90 potential CTP-binding proteins which are involved in a variety of biological processes,including protein folding,nucleotide binding and cell-cell ***,we developed a chemical proteomic method for uncovering the CTP-binding proteins in human cells,which could be widely applicable for profiling CTP-binding proteins in other biological samples.

关键词： CTP-binding proteins CTP affinity probe Mass spectrometry SIlAC Quantitative proteomics

A new endophytic insect-associated Daldinia species, recognised from a comparison of secondary metabolite profiles and molecular phylogeny

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Fungal Diversity 2013年第3期60卷 107-123页

作者： Sylvie pažoutová Stefanie Follert Jens Bitzer Matthias Keck Frank Surup PetrŠrůtka Jaroslav Holuša Marc Stadler Institute of Microbiology of the ASCR v.v.i.Vídeňská108314220 Prague 4Czech Republic Department Microbial Drugs Helmholtz-Zentrum für Infektionsforschung GmbHInhoffenstrasse 738124 BraunschweigGermany IMD Natural Solutions GmbH Otto-Hahn-Strasse 1544227 DortmundGermany Faculty of Forestry and Wood Sciences Czech University of Life SciencesKamýcká12916521 Prague 6Czech Republic

Xylariaceous endophytes are commonly encountered in plants,and it has recently been found out that insect vectors play a major role in their life cycle,as well as in the apparent host-specificity of the fungal *** this study,a new insect-associated,endophytic anamorph species of Daldinia is recognised from a comparison of (a)morphological and ecological data;(b)molecular phylogenetic data based on rDNA,alpha-actin and beta-tubulin genes;and (c)secondary metabolite profiles based on high performance liquid chromatography/diode array/mass spectrometry(HPlC-DAD/MS)and gas chromatography/mass spectrometry(GC-MS)in comparison with authentic strains of Daldinia and other *** new species,Daldinia hawksworthii,is a symbiont of the willow woodwasp,Xiphydria prolongata,which is in turn preferentially associated with Salix.A comparison of HPlCDAD/MS profiles from standardised cultures revealed the presence of a specific compound,which was not encountered in any of the ca.250 Daldinia strains studied for *** compound was isolated after fermentation of the fungus in 10 l scale by preparative *** elucidation by NMR spectroscopy revealed a novel pyrone,for which the name dalsymbiopyrone is *** compound showed weak antimicrobial and cytotoxic effects and might be a signal molecule involved in the trilateral ecological interaction between the host plant,the insect,and its fungal symbiont.

关键词： Fungi Endophytes Evolution Xylariales Bioactive metabolites Natural products Alpha-pyrones

Extraction of aluminium as aluminium sulphate from thermal power plant fly ashes

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Transactions of Nonferrous Metals Society of China 2017年第9期27卷 2082-2089页

作者： Seoul SANGITA Niva NAYAK Chitta Ranjan pa Environment & Sustainability Department CSIR-Institute of Minerals & Materials Technology

Valuable metal extraction technology from thermal power plant fly ash is *** the present study,aluminium is extracted from fly ash as highly pure aluminium sulphate(>99.0%)by leaching with sulphuric acid,followed by pre-concentration and successive *** types of fly ashes from different sources,i.e.,Talcher Thermal Power Station(TTPS)and Vedanta Aluminium Company limited(VAl)were chosen for comparative study on the extraction of aluminium as aluminium *** product is characterized by powder X-ray diffraction(XRD),Fourier transform infrared spectroscopy(FTIR)and thermogravimetric analysis(TGA).Purity of aluminium sulphate was also investigated by inductively coupled plasma?optical emission spectrometry(ICP?OES).The extraction efficiency of aluminium depends on the varied solid-to-liquid ratio(fly ash:18mol/l H2SO4,g/ml)and particle size of fly ***-chemical analysis indicates that the obtained product is Al2(SO4)3·18H2O,having low iron content(0.08%).

关键词： fly ash sulphuric acid aluminium sulphate leaching solid-to-liquid ratio particle size

下坡运动对大鼠骨骼肌肌浆网功能的影响(英文)

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中国康复医学杂志 2007年第12期22卷 1070-1074,1077页

作者：陈万 Ruell pa Thompson MW Department of Fundamental Theories Shandong Sport UniversityJinan250063China School of Exercise & Sport Science Faculty of Health SciencesThe University of SydneySydney1825NSWAustralia

目的:观测研究下坡(离心)运动对大鼠骨骼肌肌浆网Ca2+-ATP酶活性,Ca2+摄取与释放在量与时程上的影响。此外,测定离子载体的刺激作用,即测定在含与不含(Ca2+离子载体)A23187时Ca2+-ATP酶活性的比值,用以评定囊泡的完整性。方法:成年雄性S... 详细信息

目的:观测研究下坡(离心)运动对大鼠骨骼肌肌浆网Ca2+-ATP酶活性,Ca2+摄取与释放在量与时程上的影响。此外,测定离子载体的刺激作用,即测定在含与不含(Ca2+离子载体)A23187时Ca2+-ATP酶活性的比值,用以评定囊泡的完整性。方法:成年雄性SD大鼠随机分为对照与离心运动组,离心运动的大鼠分别于运动后即刻,4,24,48,72和144h后取样(n=7).离心运动方式采用90min持续跑台下坡运动(-16°;15m/min)。取大鼠红股肌制备组织匀浆,测定肌浆网Ca2+-ATP酶活性,Ca2+摄取与释放。结果:与对照组[19.25±1.38nmol·min-1·(mg protein)-1]相比,肌浆网Ca2+摄取分别于运动后即刻和4h下降了29% and 36%(Pl·min-1·(mg protein)-1]相比,也分别于运动后即刻和4h下降了37% and 39%(P<0.05),24h持续降低(P<0.05).用含离子载体测定的肌浆网Ca2+-ATP酶活性运动后4h降低了31%(P<0.05),并于运动后24h仍然降低(P<0.05)。运动后,含与不含A23187时测定的Ca2+-ATP酶活性的比值未见显著性改变,表明该运动没有明显改变肌浆网膜的完整性。结论:一次性低强度,长时间下坡运动导致肌浆网功能长时间降低,运动后恢复期两天尚未完全恢复,亦可构成离心运动诱导的骨骼肌某些功能降低的基础。提示这些变化可能产生于离心收缩时肌节长度不匀一性所造成的张力应激。

关键词：离心运动:肌浆网 Ca^2+-ATP酶活性 Ca^2+摄取 Ca^2+释放

结合U-net与FPN的地震初至波拾取算法

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测绘地理信息 2024年第1期49卷 82-87页

作者：何彬周云耀吕永清中国地震局地震研究所湖北武汉430071 中国地震局地震大地测量重点实验室湖北武汉430071

精确识别初至波以及拾取地震初至波初至时间对于地震的精确定位、地震孕震机制的解释、地震预警等都具有很大的意义。单纯地依靠地震学专家进行分析会耗费大量的人力和时间。本文将U-net模型编码、解码语义分割的思想与特征图金字塔网络... 详细信息

精确识别初至波以及拾取地震初至波初至时间对于地震的精确定位、地震孕震机制的解释、地震预警等都具有很大的意义。单纯地依靠地震学专家进行分析会耗费大量的人力和时间。本文将U-net模型编码、解码语义分割的思想与特征图金字塔网络(feature pyramid networks,FPN)的思想结合,提出了一种新的初至波初至时间拾取算法,满足高精度识别与拾取初至波的需求。算法模型分为编码、解码两部分,编码部分分为四层,用于捕获特征,通过卷积核大小、步长等参数实现特征由低层级到高层级的抽象和提取。解码部分分为四层,通过对前一层进行反卷积上采样实现。最后,将编码、解码部分对应层级特征图组合,通过两层全连接神经网络实现最终结果输出。与传统算法相比,U-net FPN模型在对初至波初至时间的分类与定位上都有着显著提升。

关键词：初至波拾取 U-net FPN

妊娠期高血压后遵循医师优化的自我管理长期血压控制:医生优化产后高血压治疗(POP-HT)随机临床试验

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中华高血压杂志（中英文） 2024年第2期32卷 179-179页

作者：袁源(译) 郑武洪(审校) 不详

妊娠期高血压可致不良心脏重构,晚年高血压和心血管疾病风险升高。本研究旨在探究设计一种实现更好血压控制的产后干预方案,能否比常规门诊护理在产后头9月期间获得更低的血压。设计、设置和参与者:在英国一家医院中设计随机、开放标签... 详细信息

妊娠期高血压可致不良心脏重构,晚年高血压和心血管疾病风险升高。本研究旨在探究设计一种实现更好血压控制的产后干预方案,能否比常规门诊护理在产后头9月期间获得更低的血压。设计、设置和参与者:在英国一家医院中设计随机、开放标签、盲法、终点试验。纳入年龄≥18岁,妊娠子痫前期或妊娠期高血压后,产后出院需要降压药治疗的女性为研究对象。

关键词：妊娠期高血压随机临床试验血压控制降压药治疗门诊护理心脏重构高血压治疗盲法

Design of a Sliding Mode Control for a DC-to-DC Buck-Boost Converter

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Design of a Sliding Mode Control for a DC-to-DC Buck-Boost C...