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Two nanobody‑based immunoassays to differentiate antibodies against genotype 1 and 2 porcine reproductive and respiratory syndrome virus

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Animal Diseases 2024年第2期4卷 98-114页

作者： Xu Chen Yueting Chang Lu Zhang Xinyu Zhao Zhihan Li Zhijie Zhang Pinpin ji qingyuan Liu jiakai Zhao jiahong Zhu Baoyuan Liu Xinjie Wang Yani Sun qin Zhao Department of Preventive Veterinary Medicine College of Veterinary MedicineNorthwest A&F UniversityYanglingShaanxi 712100China Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518100China

Porcine reproductive and respiratory syndrome virus(PRRSV)infection causes significant economic loss to the global pig *** 1 and 2 PRRSV(PRRSV-1 and-2)infections have been reported in China,Europe and *** accurate prevention,nanobodies were first used as diagnostic reagents for PRRSV *** this study three nanobodies targeting both PRRSV-1 and-2,two targeting PRRSV-1 and three targeting PRRSV-2,were screened and *** develop two competitive ELISAs(cELISAs),the g1-2-PRRSV-Nb3-HRP nanobody was chosen for the g1-2-cELISA,to detect common antibodies against PRRSV-1 and-2,and the g1-PRRSV-Nb136-HRP nanobody was chosen for the g1-cELISA,to detect anti-PRRSV-1 *** two cELISAs were developed using PRRSV-1-N protein as coating antigen,and the amounts for both were 100 ng/*** optimized dilution of testing pig sera was 1:20,the optimized reaction times were 30 min,and the colorimetric reaction times were 15 ***,the cut-off values of the g1-2-cELISA and g1-cELISA were 26.6%and 35.6%,*** of them have high sensitivity,strong specificity,good repeatability,and *** addition,for the 1534 clinical pig sera,an agreement rate of 99.02%(Kappa values=0.97)was determined between the g1-2-cELISA and the commercial IDEXX ELISA *** the g1-cELSIA,it can specifically detect anti-PRRSV-1 antibodies in the clinical pig ***,combining two nanobody-based cELISAs can differentially detect antibodies against PRRSV-1 and-2.

关键词： PRRSV Competitive ELISA Nanobody Antigen epitope

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Polycrystalline ZSM-5 Aggregates Induced by Seed and Catalytic Performance in Methanol to Hydrocarbon

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无机材料学报 2024年第8期39卷 945-954,I0010-I0013页

作者： WANG Xuchang jiAO Chuyu ji Zhuo jiAO Qirui qin Bo DU Yanze ZHENG jiajun LI Ruifeng Key Laboratory of Coal Science and Technology MOE Research Centre of Energy Chemical&Catalytic TechnologyTaiyuan University of TechnologyTaiyuan 030024China SINOPEC Dalian Research Institute of Petroleum and Petrochemicals Co. Ltd.SINOPECDalian 116045China

Synthesis of ZSM-5 zeolite typically utilizes small molecule polyamines or quaternary ammonium salts as organic structure guiding agent(OSDA).By contrast,the OSDA-free hydrothermal synthesis system eliminates the use of organic templates and the subsequent calcination *** not only reduces the cost of synthesis,but also prevents environmental pollution from the combustion of organic templates,representing an eco-friendly *** this,literature suggests that even so-called template-free synthesis systems often involve trace amount of organic substances like *** the present work,a calcined commercial ZSM-5 zeolite was served as seed,with sodium aluminate as aluminum source and silica sol as silicon source,ensuring an entirely template-free synthesis *** ZSM-5 aggregates consisted of rod-like nanocrystals were successfully prepared in the completely OSDA-free *** of the Si/Al ratio in ZSM-5 seed,dosage and crystallization conditions such as crystallization temperature and crystallization time on ZSM-5 synthesis were *** results show that a highly crystallinity ZSM-5 aggregate consisting of primary nano-sized crystals less than 100 nm is produced from a gel precursor with 5.6%(in mass)seed after hydrothermal treatment for 48 ***,the Si/Al ratio in ZSM-5 seed has little effect on the topological structure and pore structure of the synthesized ***,the seeds with a low Si/Al ratio facilitate faster crystallization of zeolite and enhance the acidity,especially the strong acid centers,of the *** catalytic performance of the synthesized polycrystalline ZSM-5 was evaluated during dehydration of methanol and compared with a commercial reference *** results exhibit that as compared with the reference catalyst,the fabricated sample has a longer catalytic lifetime(16 h vs 8 h)attributed to its hierarchical pores derived from the loosely packed primary ***

关键词： ZSM-5 polycrystalline aggregate crystal seed structure guiding agent

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数字经济与绿色发展耦合协调的时空演化及障碍度分析

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华东经济管理 2024年第7期38卷 77-90页

作者：姬新龙刘琴兰州财经大学金融学院甘肃兰州730020

数字经济不断赋能绿色发展,已经成为促进国民经济绿色转型的重要引擎。文章选取2011—2021年中国30个省份的样本数据,对数字经济与绿色发展耦合协调的时空演化及障碍度进行实证分析。研究发现:我国数字经济和绿色发展的综合水平呈明显... 详细信息

数字经济不断赋能绿色发展,已经成为促进国民经济绿色转型的重要引擎。文章选取2011—2021年中国30个省份的样本数据,对数字经济与绿色发展耦合协调的时空演化及障碍度进行实证分析。研究发现:我国数字经济和绿色发展的综合水平呈明显上升趋势;二者的耦合协调度在空间上具有显著集聚特征,并呈现由东向西、由南向北降低的趋势;影响二者耦合协调的准则层障碍因子按障碍度由大到小排序,依次为数字产业化、绿色生态、产业数字化、绿色生产、绿色生活,且不同时间和地区指标层的障碍因子也存在差异。由此提出加大政策扶持力度、夯实数字经济底座、因地制宜实施发展策略的对策建议。

关键词：数字经济绿色发展耦合协调障碍度数字产业化产业数字化

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Different coalescence sources of light nucleus production in Au-Au collisions at √sNN=3 GeV

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Chinese Physics C 2024年第5期48卷 160-179页

作者：王瑞芹吕济鹏李彦豪宋军邵凤兰 School of Physics and Physical Engineering Qufu Normal UniversityQufu 273165China School of Physical Science and Intelligent Engineering Jining UniversityJining 273155China

We study the production of light nuclei in the coalescence mechanism of Au-Au collisions at midrapidity at √sNN=*** derive analytic formulas of the momentum distributions of two bodies,three bodies,and four nucleons coalescing into light nuclei and naturally explain the transverse momentum spectra of the deuteron(d),triton(t),helium-3(3He),and helium-4(4He).We reproduce data on the yield rapidity densities,yield ratios,and averaged transverse momenta of d,t,3He,and 4He and provide the proportions of contributions from different coalescence sources for t,3He,and 4He in their *** find that besides nucleon coalescence,nucleon+nucleus coalescence and nucleus+nucleus coalescence may play requisite roles in light nucleus production in Au-Au collisions at √sNN=3 GeV.

关键词： light nucleus production the coalescence model relativistic heavy ion collision

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空气过滤用静电纺纳米纤维材料研究进展

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纺织学报 2024年第8期45卷 35-43页

作者：刘嘉炜季东晓覃小红东华大学纺织学院上海201620 东华大学纺织科技创新中心上海201620

面对日益严重的空气污染,传统过滤材料的过滤效率低且过滤阻力大,静电纺纳米纤维材料具有高孔隙率、大比表面积等特性,过滤效率高且过滤阻力小,可广泛应用于空气过滤及个人防护等领域。为此,首先从加工方式方面综述了3种静电纺丝法,不... 详细信息

面对日益严重的空气污染,传统过滤材料的过滤效率低且过滤阻力大,静电纺纳米纤维材料具有高孔隙率、大比表面积等特性,过滤效率高且过滤阻力小,可广泛应用于空气过滤及个人防护等领域。为此,首先从加工方式方面综述了3种静电纺丝法,不同的方法可获得不同尺度的纤维;其次,从不同纳米纤维材料结构和功能化制备角度,系统综述了空气过滤纳米纤维材料的最新研究进展;最后对规模化制备及纺丝原理进行论述,并展望了未来发展趋势。研究认为:需要深入研究静电纺丝机制,进一步研究电场、溶液、纤维成形之间的影响机制与构效关系开发新技术,来实现静电纺纳米纤维材料的规模化宏量制备;在此基础上对其进行功能化改进,研发具备自清洁、抗菌和传感等功能的空气过滤纳米纤维材料,以提高其在空气过滤领域的应用价值。

关键词：空气过滤静电纺丝纳米纤维材料规模化制备功能化

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Effects of ginkgo flavone aglycone on atherosclerosis based on network pharmacology,molecular docking,and in vitro experiments

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TMR Modern Herbal Medicine 2024年第2期7卷 1-10页

作者： Miao Zhou Rui Li qin Li Yan-Li Huang Shi-jing Liu ji-Yu Chen Yan He Animal Core Facility Nanjing Medical UniversityNanjing 211166China The Animal Experimental Center China Pharmaceutical UniversityNanjing 211166China Clinical Trials Center The Affiliated Hospital of Guizhou Medical UniversityGuiyang 550000China Key Laboratory of Basic Pharmacology of Ministry of Education Zunyi Medical UniversityZunyi 563000China Office of Academic Research The Affiliated Hospital of Guizhou Medical UniversityGuiyang 550000China

Background:Ginkgo flavone aglycones(GA),a Ginkgo(Ginkgo biloba)extract,has been proven to have good biological activity in atherosclerosis(AS)***,its active compounds and the corresponding mechanism for the treatment of AS remain ***:To evaluate and identify the potential pharmacological mechanisms of GA in AS treatment,the program Cytoscape was used to generate network mappings of the GA-AS-potential target *** and KEGG enrichment analyses were performed to further investigate the potential mechanism of AS and the pharmacological properties of GA.A molecular docking approach was utilized to determine the GA components that interact with *** vitro experiments were carried out to identify the anti-atherosclerotic effects of GA by targeting ***:Network pharmacological research determined that the active components of GA(quercetin,kaempferol,and isorhamnetin)correlated with AS target genes such as AKT1,EGFR,SRC,ESR1,PTGS2,MMP9,KDR,GSK3B,APP,and MMP2,*** enrichment and KEGG analysis showed that PI3K-Akt signaling may play an important role in GA *** docking experiments indicated that quercetin,kaempferol,and isorhamnetin integrate into the binding pockets of the most potentially beneficial GA-AS target protein(Akt).Consequently,cell experiments were conducted to support the anti-atherosclerotic activity of GA on AS by inhibiting the phosphorylation of AKT1 and its downstream signaling molecules,which regulated the proliferation of ***:Our results detailed GA's active ingredients,potential targets,and molecular basis against *** may exert anti-atherosclerotic effects by suppressing Akt phosphorylation and inhibiting the proliferation of *** also proposed a viable approach to determining the scientific foundation and therapeutic mechanism of Chinese herbal medicine extracts in disease therapy.

关键词： network pharmacology ginkgo flavone aglycones atherosclerosis molecular docking kaempferol quercetin isorhamnetin

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Banxia xiexin decoction prevents the development of gastric cancer

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World Journal of Clinical Oncology 2024年第10期15卷 1293-1308页

作者： Guo-Xiu Zu Ke-Yun Sun Xi-jian Liu ji-qin Tang Hai-Liang Huang Tao Han College of Traditional Chinese Medicine Shandong University of Traditional Chinese MedicineJinan 250355Shandong ProvinceChina School of Rehabilitation Medicine Shandong University of Traditional Chinese MedicineJinan 250355Shandong ProvinceChina

BACKGROUND In China banxia xiexin decoction(BXD)has been used in treating gastric cancer(GC)for thousands of years and BXD has a good role in reversing GC histopathology,but its chemical composition and action mechanism are still *** To investigate the mechanism of action of BXD against GC based on transcriptomics,network pharmacology,in vivo and in vitro *** The transplanted tumor model was prepared,and the nude mouse were pathologically examined after administration,and hematoxylin-eosin staining was *** active ingredients of BXD were quality controlled and identified using ultra-performance liquid chromatography tandem quadrupole electrostatic field orbitrap mass spectrometry(UPLC-Q-Orbitrap MS/MS),and traditional Chinese medicines systems pharmacology platform,drug bank and the Swiss target prediction platform to predict the relevant targets,the differentially expressed genes(DEGs)of GC were screened by RNA-seq sequencing,and the overlapping targets were analyzed to obtain the key targets and *** Counting Kit-8,apoptosis assay,cell migration and Realtime fluorescence quantitative polymerase chain reaction were used for in vitro *** All dosing groups inhibited the growth of transplanted tumors in laboratory-bred strain nude,with the capecitabine group and the BXD medium-dose group being the best.A total of 29 compounds and 859 potential targets in BXD were identified by UPLC-Q-Orbitrap MS/MS and network pharmacology,RNA-seq sequencing found 4767 GC DEGs,which were combined with network pharmacology and analyzed 246 potential therapeutic targets were obtained and pathway results showed that BXD may against GC through the Phosphoinositide 3-kinase(PI3K)/protein kinase B(AKt)signaling *** vitro cellular experiments confirmed that BXDcontaining serum and LY294002 could inhibit the proliferation of GC cells,promote apoptosis,and inhibit the migration of GC cells by decreasing the expression of EGFR,PIK

关键词： Banxia xiexin decoction Gastric cancer Ultra-performance liquid chromatography tandem quadrupole elec-trostatic field orbitrap mass spectrometry Network pharmacology Whole transcriptomic sequencing Phosphoinositide 3-kinase/protein kinase B signaling pathway

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地方政府举债融资机制与隐性债务管理——基于对撤县设区改革中政府举债行为的分析

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中国农村经济 2024年第3期 166-184页

作者：吉富星洪源秦玉奇中国社会科学院大学应用经济学院湖南大学经济与贸易学院湖南工商大学财政金融学院

本文实证检验了撤县设区改革通过地方政府举债融资机制对地方隐性债务规模扩张的影响。结果显示:撤县设区改革能够通过影响政府举债融资机制供需两方面的土地融资杠杆和投资竞争需求,引起地方隐性债务规模的扩张,同时在项目、资金层面... 详细信息

本文实证检验了撤县设区改革通过地方政府举债融资机制对地方隐性债务规模扩张的影响。结果显示:撤县设区改革能够通过影响政府举债融资机制供需两方面的土地融资杠杆和投资竞争需求,引起地方隐性债务规模的扩张,同时在项目、资金层面形成多个作用路径。从债务监管环境看,债务监管越严,越能抑制撤县设区改革对隐性债务规模扩张的作用。进一步分析发现,受显性债务替代效应和隐性债务增量压缩效应影响,地方债务“显性化”政策能抑制撤县设区改革对隐性债务规模的扩张效应。上述结论对于防范化解地方债务风险以及实现新型城镇化具有政策启示:应审慎推进行政区划调整模式下的城镇化,开好举债“前门”、推动融资平台转型和项目合规融资,降低土地财政依赖,加强债务监管,构建更有效防范化解地方债务风险的长效机制。

关键词：举债融资机制地方隐性债务撤县设区改革

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CFRP-不锈钢夹层管混凝土柱轴压试验及承载力计算方法

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华南理工大学学报（自然科学版） 2024年第1期52卷 1-14页

作者：陈宗平覃伟恒梁宇涵周济广西大学土木建筑工程学院广西南宁530004 南宁学院土木与建筑工程学院广西南宁530200 广西大学工程防灾与结构安全教育部重点实验室广西南宁530004

为了提升薄壁不锈钢结构的承载性能和耐久性,在不锈钢管内外壁粘贴碳纤维增强复合材料(Carbon Fiber-Reinforced Polymer,CFRP)复合约束海水海砂混凝土,制得一种CFRP-不锈钢夹层管海水海砂混凝土结构。以CFRP粘贴层数和方式为变化参数,... 详细信息

为了提升薄壁不锈钢结构的承载性能和耐久性,在不锈钢管内外壁粘贴碳纤维增强复合材料(Carbon Fiber-Reinforced Polymer,CFRP)复合约束海水海砂混凝土,制得一种CFRP-不锈钢夹层管海水海砂混凝土结构。以CFRP粘贴层数和方式为变化参数,对18个短柱试件进行了单调轴心受压试验,观察了试件的受力破坏过程及形态,获取了荷载-位移曲线和材料应变分布数据,分析了试件轴压力学性能的变化规律。结果表明:内外贴CFRP能有效地提高结构的承载能力和变形能力;无CFRP和内贴CFRP试件的破坏形态均为剪切破坏,但破坏形态会随着外贴CFRP层数的增加向腰鼓破坏转变;在相同粘贴方式下,试件的承载能力、变形能力和耗能能力随着CFRP层数的增加呈非线性提高;在相同粘贴层数下,内贴CFRP的力学性能提升效果优于外贴CFRP,粘贴1层和2层CFRP时,内贴试件的极限承载力相较于外贴试件能再提高5.6%和6.7%,同时内贴1层CFRP的试件的耗能能力与外贴2层CFRP的相当;应变分析显示,在试件破坏前,CFRP与不锈钢协同工作良好。文中最后基于极限平衡法,考虑不锈钢的应变硬化效应,提出了CFRP-不锈钢夹层管混凝土柱极限轴压承载力的计算公式,并收集了73个样本的数据进行验证,发现计算值与试验值较为吻合。

关键词：碳纤维增强复合材料不锈钢海水海砂混凝土单调轴压能量耗散承载力计算

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异常信息敏感的框架API生命周期模型构造

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计算机学报 2024年第9期47卷 1989-2008页

作者：燕季薇黄进豪杨恒钦严俊中国科学院软件研究所软件工程技术研究开发中心北京100190 北京工业大学信息学部北京100124 国科大杭州高等研究院杭州310024 中国科学院软件研究所基础软件与系统重点实验室北京100190 中国科学院软件研究所计算机科学国家重点实验室北京100190

大型软件系统的实现依赖于底层框架或第三方库,但这些复杂的框架/库代码在演化升级时往往独立于其调用者,为上层软件的质量保障带来挑战.例如,框架/库代码演化时新增和删除API、更改API的代码语义等行为会导致框架/库代码的不同版本之... 详细信息

大型软件系统的实现依赖于底层框架或第三方库,但这些复杂的框架/库代码在演化升级时往往独立于其调用者,为上层软件的质量保障带来挑战.例如,框架/库代码演化时新增和删除API、更改API的代码语义等行为会导致框架/库代码的不同版本之间不兼容,进而在上层应用开发者更新版本时,影响应用代码的正确性.为应对这一问题,需精准提取框架/库代码API的演化过程,形成演化报告,协助上层应用开发者选择兼容的版本或快速进行代码适配.其中,框架/库代码API的演化过程分析对应着框架API生命周期模型构造.现有工作中的API生命周期模型主要关注API的存在性变动,而未考虑特定代码语义变更对开发者的影响,特别是异常相关代码带来的语义变更,给上层软件系统带来隐患.为此,本文采用面向Java字节码的静态分析方法,识别框架API中的异常抛出行为并为其生成异常摘要报告,通过多轮流式匹配策略获取异常信息的变更情况,最终为框架/库代码构造异常信息敏感的API生命周期模型.该方法:(1)通过控制依赖语句切片提取异常抛出语句的关键触发条件,采用参数推断策略将局部变量的约束条件转换为仅与外部输入参数相关的异常前断言,并基于自底向上的摘要传递实现跨过程异常摘要提取;(2)通过关键信息精准匹配和自适应模糊匹配策略,分析异常摘要信息的新增、删除和修改情况,最终得到异常敏感的API生命周期模型(共涉及七种API变更形式).基于该方法,实现了基于Java字节码分析的API生命周期提取工具JavaExP.与现有最新方法相比,JavaExP的异常摘要信息提取准确性(F1值)提高了67%,分析用时减少了87%.对真实项目的API生命周期演化分析表明,与异常不敏感的API生命周期模型相比,采用异常敏感的模型时,API发生变动的比例提高了18%.在75,433个被分析的API中,约有20%API的异常抛出行为至少发生过一次改变,这些API共涉及超过七千多处独立的异常变更.在多个项目上的分析结果表明,异常敏感的模型构造能够更加精准地描述API的演化过程.

关键词：静态分析代码演化 Java异常摘要 API生命周期

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