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Review:Anaerobic ammonium oxidation for treatment of ammonium-rich wastewaters

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第5期9卷 416-426页

作者： Lei ZHANG ping ZHENG chong-jian TANG Ren-cun JIN Department of Environmental Engineering Zhejiang University Hangzhou 310029 China

The concept of anaerobic ammonium oxidation(ANAMMOX) is presently of great interest. The functional bacteria belonging to the Planctomycete phylum and their metabolism are investigated by microbiologists. Meanwhile,the ANAMMOX is equally valuable in treatment of ammonium-rich wastewaters. Related processes including partial nitritation-ANAMMOX and completely autotrophic nitrogen removal over nitrite(CANON) have been developed,and lab-scale experiments proved that both processes were quite feasible in engineering with appropriate control. Successful full-scale practice in the Netherlands will ac-celerate application of the process in future. This review introduces the microbiology and more focuses on application of the ANAMMOX process.

关键词： Anaerobic ammonium oxidation (ANAMMOX) bacteria Metabolism Partial nitritation-ANAMMOX Completelyautotrophic nitrogen removal over nitrite (CANON) Application

Severe Type of COVID-19:Pathogenesis,Warning Indicators and Treatment

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Chinese Journal of Integrative Medicine 2022年第1期28卷 3-11页

作者： SHI Ke LIU Yao ZHANG Qun RAN chong-ping HOU Jie ZHANG Yi WANG Xian-bo Center of Integrative Medicine Beijing Ditan HospitalCapital Medical UniversityBeijing(100015)China Department of Gastroenterology Dongzhimen HospitalBeijing University of Chinese MedicineBeijing(100007)China

Coronavirus disease 2019(COVID-19),caused by severe acute respiratory syndrome coronavirus 2,is a major public health *** epidemic is unlikely to be contained until the global launch of safe and effective vaccines that could prevent serious illnesses and provide herd *** most patients have mild flu-like symptoms,some develop severe illnesses accompanied by multiple organ *** identification of pathophysiology and early warning biomarkers of a severe type of COVID-19 contribute to the treatment and prevention of serious ***,we review the pathophysiology,early warning indicators,and effective treatment of Chinese and Western Medicine for patients with a severe type of COVID-19.

关键词： coronavirus disease 2019 pathogenesis indicators treatment Chinese medicine

Preliminary Estimation of Moisture Exchange in Rongbuk Valley on the Northern Slope of Mt. Qomolangma

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Atmospheric and Oceanic Science Letters 2009年第1期2卷 40-44页

作者： Ma Shu-Po Zhou Li-Bo Zou Han Ji chong-ping LAPC & LOAR Institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029 China Institute of Tibetan Plateau Research Chinese Academy of Sciences Beijing 100086 China Beijing Meteorological Observatory Beijing 100089 China

Using observed wind and water vapor data from June 2006,water vapor exchange between the Rongbuk Valley and its above atmosphere is estimated for the first *** water vapor level shows a high value from 23-29 June and a low from 12-21 June,which co-incide with the South Asian summer monsoon (SASM) active and break stages,*** water vapor can be strongly injected into the closed region of the Rongbuk Valley from the outside atmosphere,with an average strength of 0.4 g s-1 m-2 in June 2006,given that no evaporation *** air moisture exchange proc-esses can be greatly affected by the SASM evolution through changes in local radiation forcing.

关键词： the Himalayas Mt.Qomolangma moisture exchange the South Asian summer monsoon

Rescue of male infertility through correcting a genetic mutation causing meiotic arrest in spermatogonial stem cells

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Asian Journal of Andrology 2021年第6期23卷 590-599页

作者： Ying-Hua Wang Meng Yan Xi Zhang Xin-Yu Liu Yi-Fu Ding chong-ping Lai Ming-Han Tong Jin-Song Li State Key Laboratory of Cell Biology Shanghai Key Laboratory of Molecular AndrologyShanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China State Key Laboratory of Molecular Biology Shanghai Key Laboratory of Molecular AndrologyShanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesShanghai 200031China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China School of Life Science Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China

Azoospermia patients who carry a monogenetic mutation that causes meiotic arrest may have their biological child through genetic correction in spermatogonial stem cells(SSCs).However,such therapy for infertility has not been experimentally investigated *** this study,a mouse model with an X-linked testis-expressed 11(TEX11)mutation(Tex11PM/Y)identified in azoospermia patients exhibited meiotic arrest due to aberrant chromosome ***11PM/Y SSCs could be isolated and expanded in vitro normally,and the mutation was corrected by clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated endonuclease 9(Cas9),leading to the generation of repaired SSC ***-genome sequencing demonstrated that the mutation rate in repaired SSCs is comparable with that of autonomous mutation in untreated Tex11PM/Y SSCs,and no predicted off-target sites are *** SSCs could restore spermatogenesis in infertile males and give rise to fertile offspring at a high *** summary,our study establishes a paradigm for the treatment of male azoospermia by combining in vitro expansion of SSCs and gene therapy.

关键词： azoospermia gene therapy male infertility meiotic arrest spermatogonial stem cells testis-expressed 11

Quantitative analysis of magnetic anomaly of reinforcements in bored in-situ concrete piles

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Applied Geophysics 2009年第3期6卷 275-286,301页

作者： Sun Bin Dong ping Wang chong Pu Xiaoxuan Wu Yongjing Department of Earth Sciences Nanjing University Nanjing 210093 China

We quantitatively study magnetic anomalies of reinforcement rods in bored insitu concrete piles for the first time and summarized their magnetic anomaly character. Key factors such as measuring borehole orientation, borehole-reinforcement distance, and multiple-section reinforcement rods are discussed which contributes valid and quantitative reference for using the magnetic method to detect reinforcement rods. Through tests with model piles, we confirm the accuracy of theoretical computations and then utilize the law discovered in theoretical computations to explain the characteristics of the actual testing curves. The results show that the Za curves of the reinforcement rod reflect important factors regarding the reinforcement rods, such as rod length, change of reinforcement ratio, length of overlap, and etc. This research perfects the magnetic method for detecting reinforcement rods in bored in-situ concrete piles and the method has great importance for preventing building contractor fraud.

关键词： Applied geophysics concrete piles reinforcement rods magnetic method

Evaluation of a multi-site weather generator in simulating precipitation in the Qiantang River Basin, East China

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2014年第3期15卷 219-230页

作者： Yue-ping XU chong MA Su-li PAN Qian ZHU Qi-hua RAN Institute of Hydrology and Water Resources Civil Engineering Zhejiang University Hangzhou 310058 China

Recent years have seen a surge in assessment of potential impacts of climate change. As one of the most important tools for generating synthetic hydrological model inputs, weather generators have played an important role in climate change impact analysis of water management. However, most weather generators like statistical downscaling model （SDSM） and long Ashton research station weather generator （LARS-WG） are designed for single site data generation. Considering the significance of spatial correlations of hydro-meteorological data, multi-site weather data generation becomes a necessity. In this study we aim to evaluate the performance of a new multi-site stochastic model, geo-spatial temporal weather generator （GIST）, in simulating precipitation in the Qiantang River Basin, East China. The correlation matrix, precipitation amount and occurrence of observed and GiST-generated data are first compared for the evaluation process. Then we use the GiST model combined with the change factor method （CFM） to investigate future changes of precipitation （2071 2100） in the study area using one global climate model, Hadgem2 ES, and an extreme emission scenario RCP 8.5, The final results show that the simulated precipitation amount and occurrence by GiST matched their historical counterparts reasonably. The correlation coefficients between simulated and his- torical precipitations show good consistence as well. Compared with the baseline period （1961 1990）, precipitation in the future time period （2071-2100） at high elevation stations will probably increase while at other stations decreases will occur. This study implies potential application of the GiST stochastic model in investigating the impact of climate change on hydrology and water resources.

关键词： Climate change Change factor method (CFM) Multi-site weather generator Qiantang River Basin

cDNA Cloning of c33-c Antigen Gene Derived From NS3 Region of Chinese HCV Genome, Expression in Escherichia coli and Development of HCV EIA Second-Generation Diagnostic Kit

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Science China Chemistry 1994年第2期37卷 190-202页

作者： YANG Yong-ping LIU chong-Bai JIN Dong-YanZHAN Mei-Yun TANG Quan XIA Ning-ShaoCAO Jing-Yuan and LI Jing-Yuan Institute of Virology Chinese Academy of Preventive Medicine Beijing 100052 PRC

A cDNA fragment of about 860 bp corresponding to the c33-c gene in the non-structural region 3 (NS3) of HCV genome was obtained from one plasma derived from a Chinese HCV carrier who came from Tai'an of Shandong Province, China by the application of reverse transcription (RT) and polymerase chain reaction (PCR) techniques. After the sequence of the cDNA fragment was determined and compared with the equivalent region of. the HCV-I (HCV-US) and HCV-II (HCV-BK) genomes, the nucleotide/ amino acid sequence homologies were found to be 79. 2%/91. 3% and 91. 3%/93. 9%, respectively. The prokaryotic expression vector pBV220 was employed for the overproduction of c33-c native recombinant protein in E. coli cells. The expression products were detected by enzyme-linked immunosorbent assay (ELISA) and Western blotting with antisera of chronic hepatitis C patients, and a molecular weight 31 kD of c33-c viral protein was shown to account for 14% of the total cellular soluble proteins. This product was extracted from the bacterial lysate by lysozyme, Triton X-100 and urea treatment, and purified through ion exchange chromatography. The purified c33-c protein combined with a branch peptide MAP-C-19 representing immunodominant epitopes on the nucleocapsid region of HCV genome was used to develop a Chinese HCV EIA 2nd-generation diagnostic kit for the detection of anti-HCV antibodies. Its specifity, sensitivity and re-producibility were all in keeping with the indexes of the national standard for the quality control of the HCV diagnostic kit. The agreement rate between our kit and Abbott company's HCV EIA second-generation diagnostic kit was 99. 33% , and the identified rate of positive anti-HCV of our kit was 2% more than that of the Abbott company's kit.

关键词： HCV cDNA cloning overproduction of recombinant protein expression protein purification HCV EIA diagnostic kit.

Portfolio Optimization Model with Transaction Costs

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Acta Mathematicae Applicatae Sinica 2002年第2期18卷 231-248页

作者： Shu-ping Chen, chong Li, Sheng-hong Li, Xiong-wei WuDepartment of Applied Mathematics, Zhejiang University, Hangzhou 310027, ChinaDepartment of Applied Mathematics, Southeast University, Nanjing 210096, China Department of Applied Mathematics Zhejiang University Hangzhou China Department of Applied Mathematics Southeast University Nanjing China

The purpose of the article is to formulate, under the ∞ risk measure, a model of portfolio selection with transaction costs and then investigate the optimal strategy within the proposed. The characterization of a opt... 详细信息

关键词： Transaction cost portfolio optimization model algorithm

A Major QTL, Ghd8, Plays Pleiotropic Roles in Regulating Grain Productivity, Plant Height, and Heading Date in Rice

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Molecular Plant 2011年第2期4卷 319-330页

作者： Wen-Hao Yan Peng Wang Hua-Xia Chen Hong-Ju Zhou Qiu-ping Li chong-Rong Wang Ze-Hong Ding Yu-Shan Zhang Si-Bin Yu Yong-Zhong Xing Qi-Fa Zhang National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Huazhong Agricultural University Wuhan430070 China

Rice yield and heading date are two distinct traits controlled by quantitative trait loci （QTLs）. The dissection of molecular mechanisms underlying rice yield traits is important for developing high-yielding rice varieties. Here, we report the cloning and characterization of Ghd8, a major QTL with pleiotropic effects on grain yield, heading date, and plant height. Two sets of near isogenic line populations were developed for the cloning of GhdS. Ghd8 was narrowed down to a 20-kb region containing two putative genes, of which one encodes the OsHAP3 subunit of a CCAAT-box binding protein （HAP complex）; this gene was regarded as the Ghd8 candidate. A complementary test confirmed the identity and pleiotropic effects of the gene; interestingly, the genetic effect of Ghd8 was dependent on its genetic background. By regulating Ehdl, RFT1, and Hd3a, Ghd8 delayed flowering under long-day conditions, but promoted flowering under short-day conditions. Ghd8 up-regulated MOC1, a key gene controlling tillering and branching; this increased the number of tillers, primary and secondary branches, thus producing 50% more grains per plant. The ectopic expression of Ghd8 in Arabidopsis caused early flowering by 10 d-a situation similar to the one observed by its homolog AtHAP3b, when compared to wild-type under long-day conditions; these findings indicate the conserved function of Ghd8 and AtHAP3b in flowering in Arabidopsis. Our results demonstrated the important roles of Ghd8 in rice yield formation and flowering, as well as its opposite functions in flowering between rice and Arabidopsis under long-day conditions.

关键词： Near isogenic lines CCAAT-box binding protein ectopic expression flowering pathway yield formation