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Controlled and reversible induction of differentiation and activation of adult human hepatocytes by a biphasic culture technique

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World Journal of Gastroenterology 2005年第14期11卷 2080-2087页

作者： Marcus K.H. Auth Kim A. Boost Kerstin Leckel wolf-Dietrich Beecken Tobias Engl Dietger Jonas Elsie Oppermann philip Hilgard Bernd H. Markus wolf-Otto Bechstein Roman A. Blaheta Universit(a|¨)ts-Kinderklinik Essen Abteilung für Allgemeine P(a|¨)diatrie Germany Zentrum der An(a|¨)sthesiologie und Wiederbelebung Johann wolfgang Goethe-Universit(a|¨)t Frankfurt am MainGermany Klinik für Allgemein-und Gef(a|¨)βchirurgie Germany Klinik für Urologie und Kinderurologie Zentrum der Chirurgie Johann Wolfgang Goethe-Universit(a|¨)tFrankfurt am MainGermany Klinik für Allgemein- und Gef(a|¨)βchirurgie Germany Universit(a|¨)tsklinikum Essen Abteilung für Gastroenterologie und Hepatologie Germany Klinik für Allgemein- und Viszeralchirurgie St(a|¨)dt. Klinikum Kemperhof KoblenzGermany

AIM:Clinical application of human hepatocytes (HC) is hampered by the progressive loss of growth and differentiation in vitro. The object of the study was to evaluate the effect of a biphasic culture technique on expression and activation of growth factor receptors and differentiation of human adult HC. METHODS: Isolated HC were sequentially cultured in a hormone enriched differentiation medium (DM) containing nicotinamide, insulin, transferrin, selenium, and dexame-thasone or activation medium (AM) containing hepatocyte growth factor (HGF), epidermal growth factor (EGF), and granulocyte-macrophage colony-stimulating factor (GMCSF). Expression, distribution and activation of the HC receptors (MET and EGFR) and the pattern of characteristic cytokeratin (CK) filaments were measured by fluorometry, confocal microscopy and Western blotting. RESULTS: In the biphasic culture system, HC underwent repeated cycles of activation (characterized by expression and activation of growth factor receptors) and re-differentiation (illustrated by distribution of typical filaments CK-18 but low or absent expression of CK-19). In AM increased expression of MET and EGFR was associated with receptor translocation into the cytoplasm and induction of atypical CK-19. In DM low expression of MET and EGFR was localized on the cell membrane and CK-19 was reduced. Receptor phosphorylation required embedding of HC in collagen type I gel. CONCLUSION: Control and reversible modulation of growth factor receptor activation of mature human HC can be accomplished in vitro, when defined signals from the extracellular matrix and sequential growth stimuli are provided. The biphasic technique helps overcome dedifferentiation, which occurs during continuous stimulation by means of growth factors.

关键词： Human hepatocytes Differentiation Hepatocyte growth factor Epidermal growth factor HGF receptor EGF receptor Cytokeratin Collagen gel

Dietary overlap of snow leopard and other carnivores in the Pamirs of Northwestern China

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Chinese Science Bulletin 2014年第25期59卷 3162-3168页

作者： Jun Wang Alice Laguardia Peter John Damerell philip Riordan Kun Shi The Wildlife Institute School of Nature Conservation Beijing Forestry University Wildlife Conservation Research Unit Department of ZoologyUniversity of Oxford The Recanati-Kaplan Centre AbingdonOX13 5QL UK

Information about competition between carnivore species for food within high altitude regions is *** collected from the Taxkorgan Nature Reserve,China revealed important interactions between snow leopard(Panthera uncia),grey wolf(Canis lupus),red fox(Vulpes vulpes)and their prey species,including domestic ***-four line transects were conducted in order to identify field signs of habitat occupancy and collect scats for diet *** dietary overlap was observed between all three carnivore species:snow leopard and red fox(Pianka’s index=0.96),red fox and grey wolf(Pianka’s index=0.90),snow leopard and grey wolf(Pianka’s index=0.87).Snow leopard and grey wolf showed significant predation on livestock(36.8%for snow leopard and 29.4%for grey wolf in diet composition).As a pioneering exploration of the endangered snow leopard and its relationship with other species within the alpine ecosystem under livestock grazing pressure,this study contributes a greater understanding of the relationship within carnivore guild in the Pamirs whilst providing implications for conservation planning and project implementation activities in China.

关键词：帕米尔高原食肉动物中国西北雪豹膳食动物物种自然保护区塔什库尔干

强制性运动疗法对卒中后3～9个月上肢功能恢复的效果：EXCITE随机临床试验

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国际内科双语杂志（中英文） 2007年第1期7卷 68-70页

作者： Steven L. wolf Carolee J. Winstein J. philip Miller

单中心研究显示，对于卒中后超过1年而保存部分手、腕运动功能的患者，为期2周的强制性运动疗法（CIMT）能够促进上肢功能，其效果至少可达1年。

关键词：强制性运动疗法上肢功能恢复随机临床试验卒中后单中心研究运动功能

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新疆塔什库尔干岩羊和北山羊种群密度调查

野生动物 2012年第3期33卷 113-117页

作者：王君时坤 philip Riordan 北京林业大学自然保护区学院北京100083 英国牛津大学动物系野生动物保护研究所

有蹄类动物为新疆塔什库尔干地区分布的雪豹、狼和猞猁等捕食动物提供了重要的食物资源。为了掌握该地区主要野生有蹄类动物的种群密度,于2009年2～3月通过车行样线和步行样线2种调查方式,采用距离取样方法,对岩羊和北山羊种群数量进行... 详细信息

有蹄类动物为新疆塔什库尔干地区分布的雪豹、狼和猞猁等捕食动物提供了重要的食物资源。为了掌握该地区主要野生有蹄类动物的种群密度,于2009年2～3月通过车行样线和步行样线2种调查方式,采用距离取样方法,对岩羊和北山羊种群数量进行了调查分析。车行样线调查获得的岩羊密度为1.791～6.751只/km^2,北山羊密度为0～0.140只/km^2。步行样线调查获得的密度较低,岩羊和北山羊分别为0.008～0.698只/km^2和0.023～0.252只/km^2。调查结果显示该地区岩羊和北山羊种群密度较上世纪80年代中期有所下降。

关键词：塔什库尔干有蹄类动物种群密度样线调查

A cryptic mitochondrial DNA link between North European and West African dogs

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Journal of Genetics and Genomics 2017年第3期44卷 163-170页

作者： Adeniyi C.Adeola Sheila C.Ommeh Jiao-Jiao Song S.Charles Olaogun Oscar J.Sanke Ting-Ting Yin Guo-Dong Wang Shi-Fang Wu Zhong-Yin Zhou Jacqueline K.Lichoti Bernard R.Agwanda philip M.Dawuda Robert W.Murphy Min-Sheng Peng Ya-Ping Zhang State Key Laboratory for Conservation and Utilization of Bio-Resources Yunnan University Kunming 650091 China

Domestic dogs have an ancient origin and a long history in Africa. Nevertheless, the timing and sources of their introduction into Africa remain enigmatic. Herein, we analyse variation in mitochondrial DNA（mt DNA） D-loop sequences from 345 Nigerian and 37 Kenyan village dogs plus 1530 published sequences of dogs from other parts of Africa, Europe and West Asia. All Kenyan dogs can be assigned to one of three haplogroups（matrilines; clades）： A, B, and C, while Nigerian dogs can be assigned to one of four haplogroups A, B, C, and D. None of the African dogs exhibits a matrilineal contribution from the African wolf（Canis lupus lupaster）. The genetic signal of a recent demographic expansion is detected in Nigerian dogs from West Africa. The analyses of mitochondrial genomes reveal a maternal genetic link between modern West African and North European dogs indicated by sub-haplogroup D1（but not the entire haplogroup D） coalescing around 12,000 years ago. Incorporating molecular anthropological evidence,we propose that sub-haplogroup D1 in West African dogs could be traced back to the late-glacial dispersals, potentially associated with human hunter-gatherer migration from southwestern Europe.

关键词： Dog Mitochondrial DNA Africa Haplogroup D Late-glacial dispersal Hybridization

赫尔佐格和德梅隆:石屋——在人文与非人文之间

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建筑师 2021年第2期 8-12页

作者： [瑞士]菲利普·乌尔施布隆张峰(译) 于洋(校) 瑞士苏黎世联邦理工大学建筑学院上海重峰建筑设计咨询有限公司瑞士工程师与建筑师协会瑞士Isa Sturm Urs wolf建筑事务所

本文以赫尔佐格和德梅隆在1988年完成的石屋为研究对象,探讨了两位建筑师如何处理人文与非人文、框架和装饰、薄膜与图像间的复杂关系,并由此延伸至两位建筑师对于历史的批判性设计思考。

关键词：赫尔佐格和德梅隆石屋框架装饰薄膜图像不连贯性

地质和卫生科学联合评估尼日利亚手工掘金所致的儿童铅中毒

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环境与职业医学 2013年第9期30卷 689-689页

作者： Geoffrey S.Plumlee James T.Durant Suzette A.Morman Antonio Neri Ruth E.wolf Carrie A.Dooyema philip L.Hageman Heather A.Lowers Gregory L.Fernette Gregory P.Meeker William M.Benzel Rhonda L.Driscoll Cyrus J.Berry James G.Crock Harland L.Goldstein Monique Adams Casey L.Bartrem Simba Tirima Behrooz Behbod Ian von Lindern Mary Jean Brown

[背景]2010年,无国界医生组织发现尼日利亚西北部爆发与手工黄金加工相关的铅中毒。疫情已造成约400名幼儿死亡,数千人受影响。[目的]旨在进行一项跨地质和卫生科学的评估,以明确铅的来源和暴露途径,确定更多相关毒物和高危人群,并探讨... 详细信息

[背景]2010年,无国界医生组织发现尼日利亚西北部爆发与手工黄金加工相关的铅中毒。疫情已造成约400名幼儿死亡,数千人受影响。[目的]旨在进行一项跨地质和卫生科学的评估,以明确铅的来源和暴露途径,确定更多相关毒物和高危人群,并探讨全球类似的铅中毒发生的可能性。[方法]采用不同的方法,对矿石样品、来自村庄和家庭化合物中的土壤和清扫样品、植物性食物样品进行分析。[结果]富铅金矿石在开采前的自然风化形成大量、高度胃-生物可及性的碳酸铅。在所有类型的样品中发现相同的铅矿指纹,从而确定矿石处理过程造成了极大的污染,土壤/清扫样品中的铅高达185000×10-7,植物性食品中高达145×10-7。意外摄食经过手口传播的土壤和从呼吸道排出的灰尘是主要暴露途径。食用受到加工过程污染的水和食品可能铅摄入量较小,但这些仍然是重要的暴露途径。虽然幼儿遭受了最直接和最严重的后果,但研究结果表明,年龄较大的儿童、成年工人、孕妇和哺乳期婴儿也处于铅中毒的风险之中。汞、砷、锰、锑、二氧化硅结晶暴露对健康造成额外的威胁。[结论]研究结果为尼日利亚正在进行的工作提供了信息,以评估铅污染和铅中毒、治疗受害者、减少暴露以及治理污染。矿床地质、开采前风化以及新兴的手工采矿几种因素可能会综合导致全球其他地方类似的铅中毒灾难。

关键词：儿童铅中毒尼日利亚卫生科学手工地质无国界医生组织食物样品高危人群

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音乐屋——奥尔堡创意中心

城市环境设计 2020年第4期126卷 174-185页

作者：沃尔夫·狄·普瑞克斯 Marcelo Bernardi Jesper Bork Bo Stjerne Hansen Roberta Jiraschek Daniel Krüger Carsten Laursen Martin Oberascher Florian Pfeifer Robert Pippan Marita Schnepper Sigrid Svensson Eva wolf Carola B?ker Moa Carlson Isak Foged Annina G?hwiler Patricia Gola Robin Heather Per Kruse Win Man Ruth Mandl Aline Müller Tenna Olsen Anke Pasolt Annemarie Pedersen Eva Ravnborg Carolin Schmitz-Remberg Tim Seidl Guro Sollid Michele Zanella Jan-Ruben Fischer Markus Pillhofer Markus Baumann Robin Heather Anja Sorger Bo Stjerne Thomsen Anna Wasserthal philip Wilck Blaine Lepp Talya Kozminsky Hannes Walzl Morten Grau Jensen Stephanie Neufeld Tyler Bornstein Laura Githa Julia G?rtner Jenny Draxlbauer Duccio Malagamba Martin Schubert

项目大事记/CHRONOLOGY竞赛（第一名）/Competition （1st Place）:2003一期规划设计/Layout Planning Ho M I:2003—2005项目延期/Postponement of the Project:2006—2007二期规划设计/Start of Layout Planning Ho M II:2008二期动工/Start of Construction Ho M II:2010二期竣工/Completion Ho M II:2014项目参数/PROJECT DATA建筑净面积/Net Floor Area:17 637m2建筑毛面积/Gross Floor Area:20 257m2音乐厅/Concert Hall:1 298座/1 289 Seats

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