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Effects of quantitative feed restriction and sex on carcass traits,meat quality and meat lipid profile of Morada Nova lambs

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Journal of Animal Science and Biotechnology 2017年第4期8卷 992-1003页

作者： Thiago L.A.C.de Araújo Elzania S.pereira Ivone Y.Mizubuti ana C.N.Campos Marília W.F.pereira Eduardo L.Heinzen Hilton C.R.Magalhaes Leilson R.Bezerra Luciano P da Silva Ronaldo L.Oliveira Department of Animal Science Federal University of CearaFortaleza 60356001CearaBrazil Department of Animal Science State University of LondrinaLondrina 86051990ParanáBrazil Laboratory of Sensory analysis Agency for Agricultural Research(EMBRAPA-Tropical Agroindustry)Fortaleza 60511110CearáBrazil Department of Animal Science Campus Professora Cinobelina ElvasFederal University of PiauíBom Jesus 64900000PiauiBrazil Department of Animal Science School of Veterinary Medicine and Animal Science/Federal University of BahiaSalvador CityBahia State 40.170-110Brazil

Background: An experiment was conducted to evaluate the effects of feed restriction(FR) and sex on the quantitative and qualitative carcass traits of Morada Nova lambs. Thirty-five animals with an initial body weight of 14.5 ± 0.89 kg and age of 120 d were used in a completely randomized study with a 3 × 3 factorial scheme consisting of three sexes(11 entire males, 12 castrated males and 12 females) and three levels of feeding(ad libitum – AL and 30% and 60% FR).Results: Entire males presented greater hot and cold carcass weights(P 0.05) concentrations, and the concentrations of palmitic acid, palmitoleic acid, stearic acid, oleic acid and conjugated linoleic acid were higher(P < 0.05) than those of animals with 60% FR. The meat of females had a higher ω6/ω3 ratio and lower h/H ratio, and females had greater levels of feeding. The meat of animals on the 60% FR diet had a greater ω6/ω3 ratio, lower h/H ratio and lower concentration of desirable fatty acids in addition to a greater atherogenicity index(AI) and thrombogenicity index(TI).Conclusion: Lambs of different sexes had carcasses with different quantitative traits without total influence on the chemical and physical meat characteristics. The lipid profile of the meat was less favorable to consumer health when the animals were female or submitted to 60% feed restriction.

关键词： Dietary restriction Fatty acid Hair sheep Lean meat Semi-arid condition

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Seaweeds’nutraceutical and biomedical potential in cancer therapy:a concise review

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Journal of Cancer Metastasis and Treatment 2021年第1期7卷 189-212页

作者： João Cotas Diana Pacheco ana M.M.Gonçalves Paulo Silva Loïc G.Carvalho Leonel pereira University of Coimbra MARE-Marine and Environmental Sciences CentreDepartment of Life SciencesCalçada Martim de FreitasCoimbra 3000-456Portugal Department of Biology and CESAM University of AveiroAveiro 3810-193Portugal UCIBIO-REQUIMTE Departamento de QuímicaFaculdade de Ciências e TecnologiaUniversidade Nova de LisboaCaparica 2829-516Portugal The James Hutton Institute Dundee DD25DAScotlandUK Heriot-Watt University Edinburgh EH144ASScotlandUK.

Seaweeds have been a food source since ancient times(600 BC)and are still widely used in Asia,mainly in traditional Chinese medicine and Japanese folk ***,seaweed compounds and extracts have been gaining interest from the biomedical and pharmaceutical market *** have been referenced as feasible solutions in finding new potential compounds and therapies in prevention,control,and reduction of cancer development due to the multirole of some bioactive components(e.g,phenolic compounds and sulphated polysaccharides).Moreover,seaweeds are rich in important health-promoting molecules[such as poly and highly unsaturated fatty acids(PUFAs and HUFAs),essential amino acids,vitamins,and dietary fibers]and minerals(calcium,iron,iodine,magnesium,phosphorus,potassium,zinc,copper,manganese,selenium,and fluoride).In this review,the potential therapeutic effects of seaweed in the prevention and treatment of cancer are approached,as well as nutraceutical properties of seaweed to promote cell homeostasis.

关键词： Seaweed compounds nutraceutical bioactive molecules cancer prevention cancer treatment

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Use of hybrid chitosan membranes and human mesenchymal stem cells from the Wharton jelly of umbilical cord for promoting nerve regeneration in an axonotmesis rat model

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Neural Regeneration Research 2012年第29期7卷 2247-2258页

作者： Andrea Gärtner Tiago pereira Maria Joāo Simōes Paulo AS Armada-da-Silva Miguel L França Rosa Sousa Simone Bompasso Stefania Raimondo Yuki Shirosaki Yuri Nakamura Satoshi Hayakawa Akiyoshi Osakah Beatriz Porto ana Lúcia Luís Artur SP Varejāo ana Colette Maurício Animal Science and Study Centre/Food and Agrarian Sciences and Technologies Institute Porto University4099-003 PortoPortugal Institute of Biomedical Sciences Abel Salazar Veterinary Clinics DepartmentPorto UniversityPorto 4099-003Portugal Faculty of Human Kinetics Technical University of LisbonCruz Quebrada-Dafundo1499-002Portugal Institute of Biomedical Sciences Abel Salazar Cytogenetic DepartmentPorto UniversityPorto 4099-003Portugal Neuroscience Institute of the Cavalieri Ottolenghi Foundation Orbassano 10043TurinItaly Department of Clinical and Biological Sciences University of TurinOrbassano 10010TurinItaly Graduate School of Natural Science and Technology Okayama UniversityOkayama 700-8530Japan Faculty of Engineering Okayama UniversityOkayama 700-8530Japan Department of Veterinary Sciences Research Centre in SportsHealth and Human DevelopmentUniversity of Trás-os-Montes and Alto DouroVila Real 5001-801Portugal

Many studies have been dedicated to the development of scaffolds for improving post-traumatic nerve regeneration. The goal of this study was to assess the effect on nerve regeneration, associating a hybrid chitosan membrane with non-differentiated human mesenchymal stem cells isolated from Wharton＇s jelly of umbilical cord, in peripheral nerve reconstruction after crush injury. Chromosome analysis on human mesenchymal stem cell line from Wharton＇s jelly was carried out and no structural alterations were found in metaphase. Chitosan membranes were previously tested in vitro, to assess their ability in supporting human mesenchymal stem cell survival, expansion, and differentiation. For the in vivo testing, Sasco Sprague adult rats were divided in 4 groups of 6 or 7 animals each： Group 1, sciatic axonotmesis injury without any other intervention （Group 1-Crush）; Group 2, the axonotmesis lesion of 3 mm was infiltrated with a suspension of 1 250 -1 500 human mesenchymal stem cells （total volume of 50 pL）（Group 2-CrushCell）; Group 3, axonotmesis lesion of 3 mm was enwrapped with a chitosan type Ill membrane covered with a monolayer of non-differentiated human mesenchymal stem cells （Group 3-CrushChitlllCell） and Group 4, axonotmesis lesion of 3 mm was enwrapped with a chitosan type III membrane （Group 4-CrushChiUll）. Motor and sensory functional recovery was evaluated throughout a healing period of 12 weeks using sciatic functional index, static sciatic index, extensor postural thrust, and withdrawal reflex latency. Stereological analysis was carded out on regenerated nerve fibers. Results showed that infiltration of human mesenchymal stem cells, or the combination of chitosan membrane enwrapment and human mesenchymal stem cell enrichment after nerve crush injury provide a slight advantage to post-traumatic nerve regeneration. Results obtained with chitosan type III membrane alone confirmed that they significantly improve post-traumatic axonal regrowth and may represent

关键词： stem cells mesenchymal stem cells Wharton jelly umbilical cord biomaterials chitosan axonotmesis functional analysis rat karyotype analysis stereological analysis

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Tailoring the partitioning of proteins using ionic liquids as adjuvants in polymer-polymer aqueous biphasic systems

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Green Chemical Engineering 2022年第4期3卷 328-337页

作者： Flavia F.Magalhaes Matheus M.pereira Rita de Cassia Superbi de Sousa ana P.M.Tavares Joao A.P.Coutinho Mara G.Freire CICECO-Aveiro Institute of Materials Department of ChemistryUniversity of AveiroAveiro3810-193Portugal Separation Process Laboratory(LPS) Department of Food TechnologyFederal University of Viçosa(UFV)P.H.Rolfs Av.s/n36570-000 ViçosaMGBrazil

Aqueous biphasic systems(ABS)are promising for proteins purification;however,when dealing with samples comprising several proteins,the selectivity towards a target protein is difficult to *** this work,the addition of ionic liquids(ILs)as adjuvants(5 wt%and 10 wt%)in ABS composed of polyethylene glycol(PEG)and dextran was investigated to tailor proteins partitioning between the coexisting *** liquid-liquid phase diagrams were determined at 298 K,followed by partition studies of three proteins(bovine serum albumin(BSA),immunoglobulin G(IgG),and cytochrome C(Cyt C)).Partition coefficients of IgG and BSA indicate the preference of both proteins to the dextran-rich phase,whereas Cyt C has no preferential partitioning between the *** addition of chloride-based ILs as adjuvants allows to increase or decrease the partition coefficients,thus tailoring the proteins partitioning between the *** partitioning essentially depends on the IL content in each phase,whereas Cyt C and IgG partitioning is ruled by the ILs chemical structure and established *** docking was carried out to address the ILs effect on the proteins partitioning,supporting experimental observations,while identifying the specific interactions *** partition of each protein in polymer-salt ABS with ILs as adjuvants was determined,demonstrating the higher tailoring ability of polymer-polymer ABS when adding ***,the partition of each protein in presence of the remaining ones was determined,as well as the selectivity of the studied systems to separate each pair of proteins,paving the way for their use in liquidliquid chromatography.

关键词： Aqueous biphasic systems Polymer-polymer systems Protein partition Ionic liquids Adjuvants

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Laparoscopic and endoscopic cooperative surgery for full-thickness resection and sentinel node dissection for early gastric cancer

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World Journal of Gastrointestinal Endoscopy 2022年第8期14卷 508-511页

作者： Serafino Vanella Maria Godas Joaquim Costa pereira ana pereira Ivano Apicella Francesco Crafa Department of General and Oncology Surgery St.Giuseppe Moscati HospitalAvellino 83100Italy Department of General Surgery Hospital de BragaBraga 4710-243Portugal

The endoscopic submucosal dissection(ESD)technique has become the gold standard for submucosal tumors that have negligible risk of lymph node metastasis(LNM),due to its minimal invasiveness and ability to improve quality of ***,this technique is limited in stage T1 cancers that have a low risk of *** full thickness resection can be achieved with laparoscopic endoscopic cooperative surgery(LECS),which combines laparoscopic gastric wall resection and *** LECS,the surgical margins from the tumor are clearly achieved while performing organ-preserving *** overcome the limitation of classical LECS,namely the opening of the gastric wall during the procedure,which increases the risk of peritoneal tumor seeding,non-exposed endoscopic wall-inversion surgery was *** this full-thickness resection technique,contact between the intra-abdominal space and the intragastric space was eliminated.

关键词： Endoscopic submucosal dissection Laparoscopic endoscopic cooperative surgery Non-exposed endoscopic wall-inversion surgery Early gastric cancer Nodal basin evaluation

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Social and Environmental Management in the Municipalities of Minas Gerais,Brazil

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Journal of Environmental Protection 2015年第1期6卷 64-76页

作者： Jose Roberto pereira Joao Batista Rezende ana Alice Vilas Boas Universidade Federal de Lavras LavrasMGBrazil Fundacao Joao Pinheiro Belo HorizonteMGBrazil

The objective of this study is to analyze the ability of the public management of environmental problems faced by the municipalities in the state of Minas Gerais, Brazil. The theoretical elements that underlie this article are related to environmental management and municipal public administration. We used the results of the natural vulnerability and the social potential of the indicator of municipal environmental management, as well as data on sanitation and waste disposal. The weaknesses of public administration are located in the counties of low population group. These municipalities do not have suitable structure, formal organization and power of decisiveness in dealing with environmental problems.

关键词： Municipalities Public Administration Environment Sustainability

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Skin models of cutaneous toxicity,transdermal transport and wound repair

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Burns & Trauma 2023年第1期11卷 393-407页

作者： Inês Vilela de Sousa Miguel J.S.Ferreira Luís B.Bebiano Sandra Simões ana Filipa Matos Rúben F.pereira Pedro L.Granja i3S-Instituto de Investigação e Inovação em Saúde Universidade do PortoRua Dr.Manuel Pereira da Silva4200-393 PortoPortugal INEB-Instituto de Engenharia Biomédica Universidade do PortoRua do Campo Alegre8234150-180 PortoPortugal ICBAS-Instituto de Ciências Biomédicas Abel Salazar Universidade do PortoRua de Jorge Viterbo Ferreira 2284050-313 PortoPortugal FEUP-Faculdade de Engenharia da Universidade do Porto Rua Dr.Roberto Frias4200-465 PortoPortugal Department of Mechanical Aerospace and Civil EngineeringSchool of EngineeringFaculty of Science and Engineering&Henry Royce InstituteEngineering Building AThe University of ManchesterOxford RoadManchester M139PLUK ISEP-Instituto Superior de Engenharia do Porto Universidade do PortoRua Dr.António Bernardino de Almeida 4314200-072 PortoPortugal iMed.ULisboa Faculty of PharmacyUniversidade de LisboaAv.Prof.Gama Pinto1649-003 LisboaPortugal Faculty of Pharmacy Universidade de LisboaAv.Prof.Gama Pinto1649-003 LisboaPortugal

Skin is widely used as a drug delivery route due to its easy access and the possibility of using relatively painless methods for the administration of bioactive ***,the barrier properties of the skin,along with its multilayer structure,impose severe restrictions on drug transport and ***,bioengineered models aimed at emulating the skin have been developed not only for optimizing the transdermal transport of different drugs and testing the safety and toxicity of substances but also for understanding the biological processes behind skin *** though in vivo research is often preferred to study biological processes involving the skin,in vitro and ex vivo strategies have been gaining increasing relevance in recent ***,there is a noticeably increasing adoption of in vitro and ex vivo methods by internationally accepted ***,microfluidic organ-on-a-chip devices are nowadays emerging as valuable tools for functional and behavioural skin *** in miniaturization,automation and reliability still need to be addressed in order to create skin models that can predict skin behaviour in a robust,high-throughput manner,while being compliant with regulatory issues,standards and *** this review,skin models for transdermal transport,wound repair and cutaneous toxicity will be discussed with a focus on high-throughput *** microfluidic strategies driven by advancements in microfabrication technologies will also be revised as a way to improve the efficiency of existing models,both in terms of complexity and throughput.

关键词： Skin In vitro models Ex vivo models Transdermal transport assays Wound healing assays Toxicity assays Microfluidic chip Skin-on-a-chip

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Antioxidant Effect of Bovine Plasma Proteins Modified via Maillard Reaction on n3 Fortified Beef Patties

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Food and Nutrition Sciences 2016年第8期7卷 671-681页

作者： carina Lorena Fernández Ricardo Alejandro Fogar Mirtha Marina Doval ana María Romero María Alicia Judis Food Industry II Laboratory Chaco Austral National University (UNCAUS) Presidencia Roque Sáenz Pe&#241a Chaco Argentina National Council of Scientific and Technical Investigations (CONICET) Buenos Aires Argentina

This work aimed to develop a healthier meat product, by improving nutritional properties related to lipid profile, using a Maillard reaction product (MRP) obtained from recycling blood from local slaughterhouses as antioxidant. Soybean oil was used as partial fat replacer and the lipid profile was modified as a decrease in the n6/n3 ratio (11.59 to 9.50), and atherogenic and trombogenic index (from 0.42 to 0.27 and 0.94 to 0.56 respectively) was observed. Beef patties were added with two MRP levels (1% w/w and 3% w/w). Patties with no antioxidants were used as control and patties containing 0.01% w/w of butylhydroxyanisole (BHA) were used as reference. Oxidative stability of refrigerated vacuum-packed patties was evaluated during 15 days of storage. Results showed 3% of MRP as the most appropriate concentration to prevent lipid oxidation with inhibition percentages of 75%, and 9 days as the limit time of chilled storage regarding consumers’ perceptions.

关键词： Meat Industry Waste Natural Occurring Antioxidants Lipid Oxidation Healthier Meat Commodities Functional Meat Products

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Mannosylated glycans impair normal T-cell development by reprogramming commitment and repertoire diversity

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Cellular & Molecular Immunology 2023年第8期20卷 955-968页

作者： Manuel M.Vicente Inês Alves Ângela Fernandes ana M.Dias Beatriz Santos-pereira Elena Pérez-Anton Sofia Santos Tao Yang Alexandra Correia Anja Münster-Kühnel Afonso R.M.Almeida Sarina Ravens Gabriel A.Rabinovich Manuel Vilanova ana E.Sousa SaloméS.Pinho i3S–Institute for Research and Innovation in Health University of PortoPortoPortugal Institute of Biomedical Sciences Abel Salazar(ICBAS) University of PortoPortoPortugal Graduate Program in Areas of Basic and Applied Biology(GABBA) ICBASUniversity of PortoPortoPortugal Faculty of Medicine University of PortoPortoPortugal Nephrology Department Centro Hospitalar e Universitário do PortoPortoPortugal Institute of Immunology Hannover Medical SchoolHannoverGermany Institute of Clinical Biochemistry Hannover Medical SchoolHannoverGermany Instituto de Medicina Molecular João Lobo Antunes Faculdade de MedicinaUniversidade de LisboaLisboaPortugal Laboratorio de Inmunopatología Instituto de Biología y Medicina Experimental(IBYME)Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET)Ciudad de Buenos AiresArgentina Laboratorio de Inmuno-oncología Translacional Instituto de Biología y Medicina Experimental(IBYME)Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET)Ciudad de Buenos AiresArgentina Facultad de Ciencias Exactas y Naturales(FCEyN) Universidad de Buenos AiresCiudad de Buenos AiresArgentina

T-cell development ensures the formation of diverse repertoires of T-cell receptors(TCRs)that recognize a variety of *** is a major posttranslational modification present in virtually all cells,including T-lymphocytes,that regulates activity/*** these structures are known to be involved in TCR-selection in DP thymocytes,it is unclear how glycans regulate other thymic development processes and how they influence susceptibility to ***,we discovered stage-specific glycome compositions during T-cell development in human and murine thymocytes,as well as dynamic *** restricting the N-glycosylation profile of thymocytes to high-mannose structures,using specific glycoengineered mice(Rag1CreMgat1fl/fl),we showed remarkable defects in key developmental checkpoints,includingß-selection,regulatory T-cell generation andγδT-cell development,associated with increased susceptibility to colon and kidney inflammation and *** further demonstrated that a single N-glycan antenna(modeled in Rag1CreMgat2fl/fl mice)is the sine-qua-non condition to ensure normal *** conclusion,we revealed that mannosylated thymocytes lead to a dysregulation in T-cell development that is associated with inflammation susceptibility.

关键词： N-glycosylation T-cell development Thymocytes Glycocalyx Inflammation

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How the Intercropping between Corn and Palisade Grass Cultivars Affects Forage Production and Pastures Characteristics under Grazing

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American Journal of Plant Sciences 2015年第9期6卷 1475-1482页

作者： Estella Rosseto Janusckiewicz carina Bellentani Chiarelli Duarte Castro Cunha Neto Elisamara Raposo ana Cláudia Ruggieri Department of Animal Science University of Sao Paulo FCAV/UNESP Sao Paulo Brazil University of Sao Paulo FCAV/UNESP Sao Paulo Brazil

Crop-livestock integrated systems have been identified as an excellent option to use land efficiently. This study investigates how the intercropping between corn and three palisade grass cultivars affects the species productivity and the physiological and structural characteristics of swards grazed under 95% light interception under mob stocking. Evaluations were performed before corn harvest and during three grazing cycles. Similarity between the cultivars on the proportion of corn morphological components has shown that none of the studied forage plants had influence on the growth of corn. The crop-livestock integrated system with corn modified the proportion of palisade grass cultivars’ morphological components, promoting the development of Xaraes cultivar. In the pre-grazing in the grazing cycles, considering 95% of light interception the Xaraes cultivar showed higher height (0.47 m) compared with Marandu (0.37 m) and similar to Piata (0.42 m). Forage density was higher in the second grazing cycle only when the forage mass was also greater with greater contribution of both leaves and stems compared to the other cycles. Mass of leaves, stems and dead material was similar between the cultivars both pre- and post-grazing. The corn and forage intercropping did not affect the growth and development of palisade grass pastures and it is a viable alternative for the crop-livestock integrated system. Corn production is not affected by the palisade grass cultivars but production can be affected by environmental conditions mainly rainfall. The Marandu, Xaraes and Piata cultivars can be recommended for integration farming system in crop-livestock integrated system and are recommended for sward managed under 95% light interception and 15 cm high residue.

关键词： Crop-Livestock Integrated System Marandu Piata Xaraes

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