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Rice science 2012年第4期19卷 263-268页

作者： LI Li peng wei-feng LIU Qian-qian ZHOU Jin-jun LIANG wei-hong XIE Xian-zhi High-Tech Research Center Shandong Academy of Agricultural Sciences College of Life Science Henan Normal University College of Life Sciences Shandong Normal University

The expression patterns of OsPILll, one of six putative phytochrome-interacting factors, were analyzed in different organs of transgenic tobacco （Nicotiana tabacum）. The expression of OsPIL 11 was organ-specific and was regulated by leaf development, abscisic acid （ABA）, jasmonic acid （JA） and salicylic acid （SA）. To further explore the role of OsPIL 11 in plant light signal transduction, a plant expression vector of OsPILll was constructed and introduced into tobacco. When grown under continuous red light, OsPILll-overexpressed transgenic tobacco exhibited shorter hypocotyls and larger cotyledons and leaves compared to wild-type seedlings. When grown under continuous far-red light, however, transgenic and wild-type seedlings showed similar phenotypes. These results indicate that OsPILll is involved in red light induced de-etiolation, but not in far-red light induced de-etiolation in transgenic tobacco, which lays the foundation for dissecting the function of OsPIL11 in phytochrome-mediated light signal transduction in rice.

关键词： rice phytochrome-interacting factor transgenic tobacco light signal transduction

Cloning and Expression Analysis of a Mitogen-Activated Protein Kinase Gene OsMPK14 in Rice

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Rice science 2010年第4期17卷 269-275页

作者： LIANG wei-hong BI Jia-jia peng wei-feng ZHANG Fan SHI Hong-hao LI Li College of Life Science Henan Normal University Xinxiang 453007 China

Mitogen activated-protein kinases （MAPKs） are important components in signal transduction pathways responding to various biotic and abiotic stresses. An MAPK gene, OsMPK14 （GenBank Accession No. GQ265780） from rice （Oryza sativa L.）, was cloned by RT-PCR. The full-length cDNA of OsMPK14 consists of 1660 bp in size, containing an open reading frame of 1629 bp, which encodes a 542-amino-acid polypeptide and has a typical protein kinase domain and a phosphorylation activation motif TDY. Sequence alignment and analysis revealed that OsMPK14 was located on rice chromosome 5, and composed of nine exons and eight introns in the coding region. Semi-quantitative RT-PCR was performed to detect the expression patterns of OsMPK14 in rice shoots and roots under darkness, drought, high salinity, low temperature and abscisic acid treatments. The OsMPK14 mRNA was induced by abscisic acid, low temperature and high salinity, but weakly inhibited by drought. In addition, the expression of OsMPK14 was up-regulated in roots, but down-regulated in shoots by light. The results indicate that OsMPK14 could be implicated in diverse rice stimuli-responsive signaling cascades, and its expression might be regulated by multiple factors.

关键词： rice mitogen-activated protein kinase gene gene clone abiotic stress expression analysis

Three-dimensional-fast imaging employing steady-state acquisition and T2-weighted fast spin-echo magnetic resonance sequences on visualization of cranial nerves Ⅲ-Ⅻ

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Chinese Medical Journal 2008年第3期121卷 276-279页

作者： CHENG Yu-shu ZHOU Zheng-rong peng wei-jun TANG feng Department of Diagnostic Radiology Cancer Hospital FudanUniversity Shanghai 200032 China

Because of the small diameter and complex anatomic course of the cranial nerves except for the optic nerve, trigeminal nerve, facial nerve, and cochlear and vestibular nerve, other cranial nerves are difficult to be visualized in magnetic resonance imaging （MRI） scanning with conventional thickness （5-10 mm）. With the rapid development of MRI technology high spatial resolution, three-dimensional and two-dimensional magnetic resonance imaging technologies have been used in recent years in the observation of normal and abnormal cranial nerves, including three-dimensional constructive interference in steady state （3D CISS） sequence, three- dimensional magnetization-prepared rapid gradient-echo （3D MP-RAGE） sequence, three-dimensional fast inflow with steady-state precession （3D FISP） seohuence, and some fast spin-echo （FSE） sequences. - Threedimensional fast imaging employing steady-state acquisition （3D-FIESTA） is a fast three-dimensional steady-state imaging sequence with high spatial resolution and contrast between the organizational structures. And this sequence was reportedly used in the study on the diseases in the cerebellopontine angle; inner ear and posterior fossa tumors.7＇8 However, the reports about the value of 3D-FIESTA sequence for the visualization of normal cranial nerves are still rare.

关键词： cranial nerves magnetic resonance imaging pulse sequence

Anticonvulsive and antioxidant effects of curcumin on pilocarpine-induced seizures in rats

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Chinese Medical Journal 2012年第11期125卷 1975-1979页

作者： DU peng TANG Hai-yan LI Xin LIN Hao-jie peng wei-feng MA Yu FAN wei WANG Xin Department of Neurology Zhongshan Hospital Fudan University Shanghai 200032 China Institute of Brain Science Fudan University Shanghai 200032China State Key Laboratory of Medical Neurobiology Fudan University Shanghai 200032 China

Background Curcumin, an active ingredient of turmeric with antioxidant and anti-inflammatory properties has recently been reported to have anticonvulsant effects in several animal models of epilepsy. This study aimed to investigate the effects of curcumin on the pilocarpine rat model of status epilepticus. Methods The effect of intraperitoneal administration of curcumin （30, 100, and 300 mg/kg） on pilocarpine-induced seizures in rats was tested. The correlation between seizure activity and hippocampal levels of nitric oxide synthase and free radicals was quantified. Whether curcumin treatment modulated these parameters was also investigated. Results Curcumin significantly increased seizure threshold at doses of 100 and 300 mg/kg. Rats with pilocarpine- induced seizures showed significantly elevated levels of malonaldehyde, nitric oxide synthase, and lactate dehydrogenase, but decreased levels of superoxide dismutase and glutathione compared with normal control rats. At doses of 100 and 300 mg/kg, curcumin reversed the effects of pilocarpine-induced seizures on nitric oxide synthase, lactate dehydrogenase, glutathione, and superoxide dismutase. However, curcumin did not restore the elevated malonaldehyde levels. Conclusion Curcumin has anticonvulsant activity in the pilocarpine rat model of seizures, and that modulation of free radicals and nitric oxide synthase may be involved in this effect.

关键词： curcumin pilocarpine seizure, status epilepticus nitric oxide synthase free radicals

Curcumin inhibits amygdaloid kindled seizures in rats

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Chinese Medical Journal 2009年第12期122卷 1435-1438页

作者： DU peng LI Xin LIN Hao-jie peng wei-feng LIU Jian-ying MA Yu FAN wei WANG Xin Department of Neurology Zhongshan Hospital Fudan University Shanghai 200032 China Institute of Brain Science Fudan University Shanghai 200032 China

Background Curcumin can reduce the severity of seizures induced by kainate acid （KA）, but the role of curcumin in amygdaloid kindled models is still unknown. This study aimed to explore the effect of curcumin on the development of kindling in amygdaloid kindled rats. Methods With an amygdaloid kindled Sprague-Dawley （SD） rat model and an electrophysiological method, different doses of curcumin （10 mg·kg^-1·d^-1 and 30 mg·kg^-1·d^-1 as low dose groups, 100 mg·kg^-1·d^-1 and 300 mg·kg^-1·d^-1 as high dose groups） were administrated intraperitoneally during the whole kindling days, by comparison with the course of kindling, afterdischarge （AD） thresholds and the number of ADs to reach the stages of class I to V seizures in the rats between control and experimental groups. One-way or two-way ANOVA and Fisher＇s least significant difference post hoc test were used for statistical analyses. Results Curcumin （both 100 mg·kg^-1·d^-1 and 300 mg·kg^-1·d^-1 ） significantly inhibited the behavioral seizure development in the （19.80±2.25） and （21.70±2.21） stimulations respectively required to reach the kindled state. Rats treated with 100mg·kg^-1·d^-1 curcumin 30 minutes before kindling stimulation showed an obvious increase in the stimulation current intensity required to evoke AD from （703.3±85.9） μA to （960.0±116.5） μA during the progression to class V seizures. Rats treated with 300 mg·kg^-1·d^-1 curcumin showed a significant increase in the stimulation current intensity required to evoke AD from （735.0±65.2） μA to （867.0±93.4） μA during the progression to class V seizures. Rats treated with 300mg·kg^-1·d^-1 curcumin required much more evoked ADs to reach the stage of class both IV （as （199.83±12.47） seconds） and V seizures （as （210.66±10.68） seconds）. Rats treated with 100 mg·kg^-1·d^-1 curcumin required much more evoked ADs to reach the stage of class V seizures （as （219.56±18.24） seconds）. Conclusion Our study suggests that curcumin has a potential antiepileptogenic eff

关键词： curcumin amygdala kindling, neurologic epileptogenesis

Observation of acoustic hybrid topological phases induced by the p-d orbital interactions

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 132-139页

作者： Xiao Xiang feng Gao Yu-Gui peng peng Wu Zong-Lin Li Xue-feng Zhu School of Physics and Innovation Institute Huazhong University of Science and Technology

In spinless systems,growing attention has recently been attracted to synthetic gauge fields,which redefine the fundamental crystal symmetries by utilizing the projective algebraic ***,synthetic gauge fields have been commonly investigated in single orbital systems,and explorations on hybrid orbitals remain elusive in ***,we report the experimental observation of hybrid topological phases induced by hybrid synthetic gauge flux,which is formed by the interaction between p and d orbitals embedded in acoustic *** breaking the translation symmetries of Lxand/or Ly,we unambiguously demonstrate the first-order M?bius edge states and higher-order corner *** work establishes a platform for seeking exotic topological phases induced by hybrid-orbitals interactions and initializing the framework of hybrid-orbitalsrelated topological *** applications can be anticipated in scenarios such as high-quality sensing and robust wave trapping due to the stepwise energy distribution of the hybrid topological phase.

关键词： topological insulator synthetic gauge field projective symmetry corner states

当前我国“三农”题材文学创作的现状、问题及建议

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山东大学学报（哲学社会科学版） 2015年第4期 53-61页

作者：彭维锋中国劳动关系学院文化传播学院中国劳动关系学院新农村文化研究所新疆维吾尔自治区工会干部学校

要理解当前"三农"题材文学创作的现状和问题,必须立足于当下的中国语境特别是新世纪之后中国乡村发生的结构性变化。"三农"问题的重要地位和独特历史魅力,生成了"三农"题材文学创作的丰富性。"三农"题材文学具有较为强烈的"民族寓言"属... 详细信息

要理解当前"三农"题材文学创作的现状和问题,必须立足于当下的中国语境特别是新世纪之后中国乡村发生的结构性变化。"三农"问题的重要地位和独特历史魅力,生成了"三农"题材文学创作的丰富性。"三农"题材文学具有较为强烈的"民族寓言"属性、迫切的问题意识和浓厚的意识形态诉求,创造了世界文学格局中的中国经验、中国情感和中国意象,也成为作家以文学的方式参与建设中国特色社会主义新农村的重要组成部分。在社会主义新农村和新型城镇化战略背景下,全面思考、探究和分析当下"三农"题材文学创作所存在的问题,加快其步伐、实现其变革、突破其短板,提出繁荣发展的思路建议,创造世界文学格局中的中国书写,将具有重大而深远的意义。

关键词： “三农”题材语境问题意识新农村中国经验

当代中国故乡意象的文学建构——以新时期乡村小说为中心

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河北学刊 2015年第5期35卷 85-89页

作者：彭维锋中国劳动关系学院文化传播学院北京100044

作为当下中国故乡意象的重要构成元素,当代乡村小说以文学的方式,重建了人与乡村、人与自然、人与人、人与自我之间的诗情画意关系,它既是乡村现代性发展的产物,也是乡村现代性困境的表征。文学故乡的建构包含两个向度的内容:一是乡村... 详细信息

作为当下中国故乡意象的重要构成元素,当代乡村小说以文学的方式,重建了人与乡村、人与自然、人与人、人与自我之间的诗情画意关系,它既是乡村现代性发展的产物,也是乡村现代性困境的表征。文学故乡的建构包含两个向度的内容:一是乡村生产生活的自然环境、器物生物、生存条件等实体性故乡,二是乡村的权力秩序、道德伦理、文化精神等情感性故乡。当代作家正是通过物质诗化与精神复魅的书写策略,建构起坚实厚重、丰富立体、诗情画意的故乡意象,并最终生成强烈的对抗现实、舒缓心灵的艺术功能;它既是创作主体对乡村现代性的一种深刻反思和有益补充,更是对当代中国乡村未来的一种文学设计与艺术想象。

关键词：乡村小说现代性故乡意象文学建构

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三种工况下扩底楔形桩承载特性模型试验研究

岩土力学 2017年第7期38卷 1887-1893页

作者：任连伟顾红伟彭怀风周杨河南理工大学土木工程学院河南焦作454000 河海大学岩土力学与堤坝工程教育部重点实验室江苏南京210098

扩底楔形桩具有单位材料利用率高、竖向桩侧摩阻力和桩端阻力较常规等截面桩高的技术优点。然而,针对该桩型竖向及水平向承载力的定量模型试验研究相对较少。基于模型试验方法,开展砂性土中竖向、水平向荷载以及地面堆载等不同形工况荷... 详细信息

扩底楔形桩具有单位材料利用率高、竖向桩侧摩阻力和桩端阻力较常规等截面桩高的技术优点。然而,针对该桩型竖向及水平向承载力的定量模型试验研究相对较少。基于模型试验方法,开展砂性土中竖向、水平向荷载以及地面堆载等不同形工况荷载作用下扩底楔形桩的承载特性试验,测得不同荷载等级下桩侧摩阻力、桩端阻力、侧向土压力、桩顶下拽位移以及桩身下拽力等分布规律;同时,开展等混凝土用量常规等直径桩的承载特性试验作为对比分析,初步探讨了两种桩型的受力机制与异同点。研究结果表明,试验条件下,扩底楔形桩的单桩竖向承载力约为等直径圆桩的2.33倍,表现出侧阻力和端阻力都优于圆形桩的特点;水平承载力约为等直径圆桩的1.48倍,上部直径较大和扩大头的存在提高了其水平承载性能;与等直径圆桩相比,楔形角的存在可有效降低桩顶下拽位移,可见把传统桩型转变成扩底楔形桩是降低负摩阻力对桩基影响的有效方法之一。

关键词：扩底楔形桩模型试验竖向承载力水平承载力下拽位移

雌二醇与姜黄素单用或联用对海人酸杏仁核点燃大鼠海马GAD_(67)表达的影响

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复旦学报（医学版） 2010年第4期37卷 455-458页

作者：彭伟锋郭曦汪昕杜鹏复旦大学附属中山医院神经内科上海200032 复旦大学附属中山医院分部内科上海200052

目的观察腹腔注射雌二醇(estradiol,E)或/和姜黄素(curcumin,C)后海人酸(kainic acid,KA)杏仁核点燃大鼠海马谷氨酸脱羧酶(glutamate decarboxylase,GAD)的亚型GAD67表达的变化,探讨在姜黄素干预下雌二醇对GAD67表达的影响。方法将32只... 详细信息

目的观察腹腔注射雌二醇(estradiol,E)或/和姜黄素(curcumin,C)后海人酸(kainic acid,KA)杏仁核点燃大鼠海马谷氨酸脱羧酶(glutamate decarboxylase,GAD)的亚型GAD67表达的变化,探讨在姜黄素干预下雌二醇对GAD67表达的影响。方法将32只去势术后的雌性SD大鼠分为E+KA组、C+KA组、EC+KA组和KA组,分别给予腹腔注射雌二醇或/和姜黄素,5天后均制备成KA杏仁核点燃的癫大鼠模型,采用免疫组化方法检测大鼠海马中GAD67表达的变化。结果统计KA注射对侧(左侧)大鼠海马中GAD67免疫阳性细胞的表达数。在CA1区,C+KA组GAD67的表达与KA组(P<0.01)、E+KA组(P<0.05)相比均明显增多,它在EC+KA组的表达较KA组(P<0.01)也明显增多,差异有统计学意义。在CA3和DG区,C+KA组GAD67的表达与KA组(P<0.01)、E+KA组(CA3区:P<0.01,DG区:P<0.05)、EC+KA组(P<0.05)相比均明显增多。而且在DG区,它在E+KA组的表达较KA组(P<0.05)也有明显增多。结论雌二醇或姜黄素单用时均可以上调KA杏仁核点燃大鼠海马中GAD67的表达,以姜黄素的上调作用更为明显。姜黄素干预后,雌二醇对GAD67的表达无明显影响,但两者联用时仍可使CA1区GAD67的表达明显增多。

关键词：雌二醇姜黄素癫 GAD67 大鼠