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Comparative analysis of astaxanthin and its esters in the mutant E1 of Haematococcus pluvialis and other green algae by HPLC with a C3. column

引用

Science China(Life sciences) 2008年第12期51卷 1108-1115页

作者： peng juan1, xiang wenzhou2.1,{2.,{3., chen feng1,3.& yuan jianping 4 1 south China sea institute of Oceanology, chinese academy of sciences,{12., China 2.institute of Oceanology, chinese academy of sciences, Qingdao 2.6071, China 3.School of Biological sciences, The University of Hong Kong, Hong Kong, China 4 institute of Oceanology & College of Life sciences, sun Yat-Sen University,{12., China south China sea {2. of Oceanology Chinese Academy of Sciences Guangzhou 510301 China institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China School of Biological sciences The University of Hong Kong Hong Kong China institute of Oceanology & College of Life sciences Sun Yat-Sen University Guangzhou 510301 China

A gradient reversed-phase high-performance liquid chromatography (HPLC) method using a C3. col-umn was developed for the simultaneous determination of astaxanthin, astaxanthin monoesters and astaxanthin diesters in the green algae Chlorococcum sp., Chlorella zofingiensis, Haematococcus plu-vialis and the mutant E1, which was obtained from the mutagenesis of H. pluvialis by exposure to UV-irradiation and ethyl methanesulphonate (EMS) with subsequent screening using nicotine. The re-sults showed that the contents of total astaxanthins including free astaxanthin and astaxanthin esters ranged from 1.4 to 3..9 mg/g dry biomass in these green algae. The lower total astaxanthin levels (2.mg/g dry biomass) were detected in the green algae Chlorococcum sp. and C. zofingiensis. The higher total astaxanthin levels (>16 mg/g dry biomass) were found in the green alga H. pluvialis and its mutant E1. It is notable that the mutant E1 is found to have considerably higher amounts of total astaxanthin (3..9 mg/g) as compared to the wild strain of H. pluvialis (16.1 mg/g). This indicates that UV-irradiation and EMS compound mutagenesis with subsequent screening using nicotine is an effective method for breeding of a high-producing astaxanthin strain of H. pluvialis. In addition, the green alga C. zofingien-sis had a remarkably higher percentage of astaxanthin diesters (76.3. of total astaxanthins) and a re-markably lower percentage of astaxanthin monoesters (18.0% of total astaxanthins) in comparison with H. pluvialis (3..5% for diesters and 60.9% for monoesters), the mutant E1 (49.1% and 48.1%) and Chlorococcum sp. (18.0% and 58.6%).

关键词： green algae Haematococcus pluvialis mutant astaxanthin HPLC C3. column

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