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低表达SLC1A4在卵巢癌顺铂耐药中的作用研究

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中国现代应用药学 2024年第9期41卷 1204-1213页

作者：苏兰娣彭建明包一笑孙国正周凡超许丁文扬州市职业大学医学院扬州225100

目的探讨溶质载体家族1成员4(solute carrier family 1 member 4,SLC1A4)在卵巢癌铂类化疗耐药性中的作用。方法通过GEO、TCGA数据库分析工具分析SLC1A4在卵巢癌或铂类耐药性卵巢癌中的表达;通过GEO数据库分析SLC1A4在铂类药物处理的卵... 详细信息

目的探讨溶质载体家族1成员4(solute carrier family 1 member 4,SLC1A4)在卵巢癌铂类化疗耐药性中的作用。方法通过GEO、TCGA数据库分析工具分析SLC1A4在卵巢癌或铂类耐药性卵巢癌中的表达;通过GEO数据库分析SLC1A4在铂类药物处理的卵巢癌细胞系中的表达;通过Kaplan Meier-plotter分析SLC1A4表达量与卵巢癌患者总生存期(overall survival,OS)、无疾病进展生存率(progression free survival,PFS)的相关性;通过DepMap平台分析SLC1A4基因效应与卵巢癌化疗药物敏感相关性;通过流式细胞试验和肿瘤细胞克隆集落形成试验验证低表达SLC1A4介导卵巢癌细胞顺铂耐药;通过TargetScan预测靶向于SLC1A4的微小RNA(miRNA),并在TCGA数据库卵巢癌样本中验证其相关性;运用COREMINE工具进行文本挖掘分析SLC1A4介导卵巢癌化疗耐药性的生物过程。结果SLC1A4在卵巢癌患者及铂类耐药性的卵巢癌中的显著降低(P<0.05),而且与患者的OS、PFS显著相关(P<0.05);铂类药物处理卵巢癌细胞增加SLC1A4的表达;SLC1A4对卵巢癌的基因效应与铂类药物敏感性正相关;过表达SLC1A4增加顺铂引起的卵巢癌细胞凋亡和减少肿瘤细胞克隆集落形成;hsa-let-7c-5p靶向于SLC1A4并在耐药性卵巢癌患者样本中呈显著负相关。结论低表达SLC1A4介导卵巢癌铂类耐药性,并且有可能与hsa-let-7c-5p调控有关。

关键词：溶质载体家族1成员4 卵巢癌铂类耐药性

国产龙血竭基原植物类黄酮3'-羟化酶基因(F3'H)克隆及表达分析

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分子植物育种 2024年第19期22卷 6310-6317页

作者：李爽赵宏友杨春勇王艳芳李戈彭建明严珍王延谦中国医学科学院北京协和医学院药用植物研究所云南分所云南省南药可持续利用研究重点实验室景洪666100

类黄酮3'-羟化酶(flavonoid 3'-hydroxylase,F3'H)作为植物类黄酮代谢的关键酶之一,参与龙血树中龙血竭树脂的生物合成。为了揭示国产龙血竭基原龙血树中F3'H基因序列和表达模式等信息,本研究以剑叶龙血树、海南龙血树和岩棕为研究对象... 详细信息

类黄酮3'-羟化酶(flavonoid 3'-hydroxylase,F3'H)作为植物类黄酮代谢的关键酶之一,参与龙血树中龙血竭树脂的生物合成。为了揭示国产龙血竭基原龙血树中F3'H基因序列和表达模式等信息,本研究以剑叶龙血树、海南龙血树和岩棕为研究对象,采用同源克隆法克隆3种龙血竭基原植物的F3'H基因序列并进行生物信息学分析,利用实时荧光定量PCR(RT-qPCR)法分析其在不同组织的表达量。结果表明,在3种龙血树中均获得一条编码区长1533 bp的F3'H基因序列,编码510个氨基酸残基。生物信息学分析表明,它们的序列同源性达到99.57%,氨基酸序列的理化性质、二级结构和三级结构几乎完全一致。系统进化分析表明龙血树属植物F3'H基因聚为一支且进化上非常接近,在F3'H进化树上龙血树属并未与同科植物聚为一类,单子叶植物和双子叶植物亦并未分别聚为一类。RT-qPCR分析发现,剑叶龙血树中F3'H在茎中的表达量远高于根和叶,海南龙血树和岩棕中F3'H在茎中的表达量低于根和叶。本研究有利于揭示F3'H在龙血竭生物合成中的潜在作用,为龙血竭人工结脂技术开发提供一定分子基础。

关键词：龙血树龙血竭类黄酮3'-羟化酶

Preparation of porous nanoparticle TiO_2 films for flexible dye-sensitized solar cells

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Chinese Science Bulletin 2011年第24期56卷 2649-2653页

作者： WANG Yue WU JiHuai LAN Zhang XIAO YaoMing LI QingBei peng FuGuo LIN jianming HUANG MiaoLiang Engineering Research Center of Environment-Friendly Functional Materials Ministry of Education Institute of Materials Physical ChemistryHuaqiao University Quanzhou 362021 China

A TiO2 paste was prepared by mixing commercial TiO2 (P25), ethanol, distilled water and a small amount of Ti (IV) tetrai-sopropoxide (TTIP), following by a hydrothermal treatment. Before hydrothermal treatment, a stirring for 48 h can prevent cracking TiO2 films. TTIP significantly promote the chemical connection between TiO2 particles and its adherence to the substrate, the TTIP amount of 6 mol% is suitable. UV irradiation can remove some impurities and water from the TiO2 film with an optimal time of 2 h. Transmission electron microscopy, X-ray diffraction, scanning electron microscopy and photovoltaic tests are charac- terized and measured. Shortcircuit current density, open-circuit voltage, fill factor and photoelectric conversion efficiencies for the fabricated flexible dye-sensitized solar cell are 7.20 mA cm-2, 0.769 V, 0.686 and 3.84%, respectively, under irradiation with a simulated solar light of 100 mW cm-2.

关键词：染料敏化太阳能电池纳米二氧化钛制造柔性薄膜制备透射电子显微镜 X射线衍射分析扫描电子显微镜紫外线照射

Reverse Circulation DTH Hammer Drilling Technique

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Global Geology 2011年第4期14卷 259-264页

作者： BO Kun YIN Kun peng jianming College of Construction Engineering Jilin University Changchun 130026 China

The technical characteristics and operational principal of the reverse circulation DTH hammer drilling technology is integrated introduced in this paper. Using simulation technology to assist the drilling tool design, the optimum designed DTH hammer gets stable performance, and the bit has reverse circulation ability, and further more, it has already developed serial products. The reverse circulation DTH hammer drilling technique has been fully applied in field such as the geological core exploration in complex strata, water well drilling, and large diameter rock embedding piles drilling in hard rock, and obviously, it is achieved favorable economic and social benefits.

关键词： Hollow-through DTH hammer reverse circulation reverse circulation bit simulation

基于高通量表面等离子体共振技术筛选与TPBG具有高亲和力的单克隆抗体研究

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现代医药卫生 2024年第14期40卷 2349-2352页

作者：江秀玲王晓丽胡有根彭建明严慧深扬州市职业大学医学院江苏扬州225009 武警江苏总队医院江苏扬州225009

目的探讨从制备的单克隆抗体中快速筛选出与滋养层糖蛋白(TPBG)具有高亲和力的单克隆抗体的方法。方法采用高通量表面等离子体共振技术(SPR),将不同种属的TPBG通过氨基偶联的方式固定在GLC传感器芯片上,梯度稀释的抗体作为流通相,筛选... 详细信息

目的探讨从制备的单克隆抗体中快速筛选出与滋养层糖蛋白(TPBG)具有高亲和力的单克隆抗体的方法。方法采用高通量表面等离子体共振技术(SPR),将不同种属的TPBG通过氨基偶联的方式固定在GLC传感器芯片上,梯度稀释的抗体作为流通相,筛选能高度亲和TPBG的单克隆抗体,并测定相互作用的动力学常数。抗体1结合人和食蟹猴TPBG,亲和力分别为65.0、31.6 nmol/L;抗体6结合人、小鼠、食蟹猴TPBG,亲和力分别为77.6、92.1、87.7 nmol/L。结果该研究成功筛选出2种能与TPBG特异性结合的单克隆抗体。动力学图谱显示,这2种抗体与TPBG蛋白的特异性结合稳定。结论采用SPR可筛选出与TPBG蛋白具有高亲和力的单克隆抗体,为开发出一些新型的抗肿瘤药物提供一种方法。

关键词： 5T4蛋白高通量表面等离子体共振亲和力单克隆抗体抗肿瘤药物

Evaluation of Latent Tuberculosis Infection Using the ESAT6-CFP10 Skin Test Among International Freshmen With Diverse Skin Tones at a University—Nanjing City,Jiangsu Province,China,September 2023

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China CDC weekly 2024年第35期6卷 896-900,I0001页

作者： peng Lu Rong Wang Jingjing Xu jianming Wang Fangfang Du Guozhi Wang Shiming Cheng Limei Zhu Department of Chronic Communicable Disease Jiangsu Provincial Center for Disease Control and PreventionNanjing CityJiangsu ProvinceChina Department of Epidemiology School of Public HealthNanjing Medical UniversityNanjing CityJiangsu ProvinceChina Nanjing City Center for Disease Control and Prevention Nanjing CityJiangsu ProvinceChina Chinese Antituberculosis Association BeijingChina National Institutes for Food and Drug Control BeijingChina

Introduction:Newer skin tests,including the ESAT6-CFP10(EC)skin test,were recommended for diagnosing Mycobacterium tuberculosis(***)***,no data exist assessing the diagnostic performance of the EC skin test among foreign students with different skin ***:A cohort study at Nanjing Medical University screened incoming foreign *** EC skin test was used to assess for *** infection,and results were read at 24,48,72,and 96-hours ***:Among 96 participants,*** infection rates at 24,48,72,and 96-hours post-injection were 3.13%,7.29%,13.54%,and 9.38%,*** infection rates were lower among individuals with darker skin tones,the difference was not statistically significant(P=0.186),and variations were consistent across different measurement *** analysis revealed 5.3%in the continuousincreasing group,86.5%in the low-stable group,and 5.2%in the elevated-decreasing ***,participants in the elevated-decreasing group had lighter skin tones,with trajectory patterns consistent across different skin ***:The EC skin test is safe,and redness diameter is a more reliable indicator than *** should be collected within 48 to 72 hours,with verification at 72 hours crucial if initial results are ***,skin color does not affect EC skin test outcomes.

关键词： CFP10 Skin Nanjing

Method for predicting cuttings transport using artificial neural networks in foam drilling

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Global Geology 2020年第2期23卷 116-122页

作者： PAK Kumdol peng jianming RI Jaemyong CHOE Kumhyok HO Yinchol School of Resource Exploration Engineering Kim Chaek University of TechnologyPyongyang 999093D.P.R.Korea College of Construction Engineering Jilin UniversityChangchun 130026China School of Information Science and Technology Kim Chaek University of TechnologyPyongyang 999093D.P.R.Korea

Foam is used widely in underbalanced drilling for oil and gas exploration to improve well *** prediction of the cutting transport and pressure loss in the foam drilling is an important way to prevent stuck pipe,lost circulation and to increase the rate of penetration(ROP).In foam drilling,the cuttings transport quality may be defined in terms of cuttings consistency and downhole pressure loss,which are controlled by many ***,it is very difficult to establish the mathematical equation that reflects nonlinear relationship among various *** field and experimental measurements of these parameters are time consuming and *** this study,the authors suggest a cuttings transport mathematical modeling using BPN(back propagation network),RBFN(radial basis function network)and GRNN(general regression neural network)based on various experiment data of cuttings transport of previous researchers and compared the result with experiment *** of this study show that the GRNN has a correlation coefficient of 0.99962 and an average error of 0.15 in training datasets,and a correlation coefficient of 0.99881 and an average error of 0.612 in testing datasets,which has higher accuracy and faster training velocity than the BP network or RBFN *** can be used in many mathematical problems for accurate estimation of cuttings consistency and downhole pressure loss instead of field and experimental measurements for hydraulic design in foam drilling operation.

关键词： cuttings transport underbalanced drilling foam

FGC-15D large-diameter DTH air hammer drilling system and its application in offshore rock-socketed pile hole drilling

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Global Geology 2006年第2期9卷 138-140页

作者： Kun YIN jianming peng Maosen WANG Zhanjiang ZHOU College of Construction Engineering Jilin University Changchun 130026 China

In the past two decades, numerous large-diameter rock-socketed piles were constructed in China to support foundations of skyscrapers, great bridges or to retain soil in potential geological hazard areas. However, drilling large-diameter rock-socketed pile holes with conventional drilling method such as rotary drilling or cable tool drilling is time-consuming and the cost is usually very high. In order to drill large-diameter rocksocketed pile holes faster at relatively low cost, the FGC15A large-diameter DTH air hammer drilling system was developed in 1987 and was given the second-clasa award by Ministry of Geology and Mineral Resources in 1991. Since it was innovated the drilling system has been used in more than twenty important and tough pro- jects on land, and wonderful results were acquired. At the same time the large-diameter DTH air hammer drilling system was improved continuously. The FGC15D is the latest version of the technique.

关键词： DTH air hammer drilling system pile hole drilling

Discussion of reasonable drilling parameters in impregnated diamond bit drilling

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Global Geology 2023年第2期26卷 114-121页

作者： PAK Kumdol HO Yinchol peng jianming RI Jaemyong HAN Changson School of Resource Exploration Engineering Kim Chaek University of TechnologyPyongyang 999093D.P.R.Korea College of Construction Engineering Jilin UniversityChangchun 130026China School of Information Science and Technology Kim Chaek University of TechnologyPyongyang 999093D.P.R.Korea

The impregnated diamond(ID)bit drilling is one of the main rotary drilling methods in hard rock drilling and it is widely used in mineral exploration,oil and gas exploration,mining,and construction *** this study,the quadratic polynomial model in ID bit drilling process was proposed as a function of controllable mechanical operating parameters,such as weight on bit(WOB)and revolutions per minute(RPM).Also,artificial neural networks(ANN)model for predicting the rate of penetration(ROP)was developed using datasets acquired during the drilling *** relationships among mechanical operating parameters(WOB and RPM)and ROP in ID bit drilling were analyzed using estimated quadratic polynomial model and trained ANN *** results show that ROP has an exponential relationship with WOB,whereas ROP has linear relationship with ***,the optimal regime of mechanical drilling parameters to achieve high ROP was confirmed using proposed model in combination with rock breaking principal.

关键词： rate of penetration(ROP) impregnated diamond bit drilling operating parameter artificial neural network