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Molecular and morphological characterization of stunt nematodes of wheat, maize, and rice in the savannahs of northern Nigeria

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Journal of Integrative Agriculture 2022年第2期21卷 586-595页

作者： Sulaiman ABDULSALAM peng Huan LIU Shi-ming HUANG Wen-kun KONG Ling-an PENG De-liang State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection Chinese Academy of Agricultural SciencesBeijing 100193P.R.China Division of Agricultural Colleges Department of Crop ProtectionAhmadu Bello UniversityZaria 810107Nigeria

Stunt nematodes(Tylenchorhynchus spp.)are obligate migratory root ecto-parasitic nematodes found in the fields of many cultivated crops.These nematodes,with phyto-sanitary potential,are frequently ignored or misdiagnosed as pests,and this may pose a threat to food security.The accuracy of its identification based on a morphological approach has been challenged recently,due to the overlapping of the morphological and morphometric characters of the species.Consequently,the objective of this study is to identify and characterize stunt nematodes present in 54 fields cultivated with cereal crops(wheat,maize and rice)in the savannahs of northern Nigeria,using integrative taxonomy and molecular approaches.The molecular and morphological studies identified and confirmed the presence of T.annulatus as the occurring specie in the savannahs of northern Nigeria.The phylogenetic analysis was carried out using the internal transcribed spacer(ITS)and 28S genes of ribosomal DNA further confirmed the presence of T.annulatus.The first molecular characterization and sequences of the ITS and 28S rDNA gene for T.annulatus from Nigeria were provided by this research.Also,according to our literature search,this is the first report on T.annulatus in wheat,maize and rice in the savannahs of northern Nigeria.Further study to test the pathogenicity of the parasitic nematode species found in this survey is recommended for the prioritization and development of efficient management strategies.

关键词： plant-parasitic nematodes food security Tylenchorhynchus annulatus ITS phylogeny

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The new effector AbSCP1 of foliar nematode(Aphelenchoides besseyi) is required for parasitism rice

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Journal of Integrative Agriculture 2022年第4期21卷 1084-1093页

作者： HUANG Xin CHI Yuan-kai Addisie Abate BIRHAN ZHAO Wei QI Ren-de PENG De-liang Institute of Plant Protection and Agro-products Safety Anhui Academy of Agricultural SciencesHefei 230001P.R.China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China Agricultural Biotechnology Directorate Ethiopian Biotechnology InstituteAddis Ababa 5954Ethiopia

Plant parasitic nematodes secrete effector proteins to parasitize hosts successfully. Of these proteins, serine carboxypeptidases have critical roles in pathogenicity. This study investigated the role of new effector AbSCP1 in Aphelenchoides besseyi pathogenicity. In situ hybridization and qRT-PCR analyses indicated that AbSCP1 was exclusively expressed in the esophageal glands and upregulated in juveniles. Subcellular localization assays indicated that the protein was expressed in the nucleus. The ability to hydrolyze C-terminal amino acid residues was proven for AbSCP1. Moreover, RNAi significantly reduced the expression of AbSCP1 and RNAi-treated nematodes’ reproductive potential. Pathogenicity assays on rice showed that RNAi-treated nematodes were less pathogenic than the untreated control groups. These results suggest the important role of AbSCP1 in the A. besseyi infection process.

关键词： Aphelenchoides besseyi serine carboxypeptidases effector nematode pathogenicity

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Effect of Aspergillus niger NBC001 on the soybean rhizosphere microbial community in a soybean cyst nematode-infested field

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Journal of Integrative Agriculture 2021年第12期20卷 3230-3239页

作者： JIN Na LIU Shi-ming peng Huan HUANG Wen-kun KONG Ling-an PENG De-liang department of Horticulture Beijing Vocational College of AgricultureBeijing 102442P.R.China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China

Soybean cyst nematode(SCN,Heterodera glycines Ichinohe)is one of the most important pests causing considerable damage to soybean(Glycine max(L.)Merr.)around the world.Biocontrol provides a strategy for sustainable nematode control.Previously,Aspergillus niger NBC001 was isolated from the cysts Heterodera spp.and able to control H.glycines and promote the growth of soybean in a pot experiment.In this study,the effects of NBC001 on H.glycines density and on the soybean rhizosphere microbial community in a soybean cyst nematode-infested field were studied.The results showed that NBC001 could suppress H.glycines by 31.7%in the field.High-throughput sequencing analysis showed that NBC001 had no significant influence on soybean rhizosphere microbial community structure,indicating that seed coatdressing with the concentrated culture filtrate of NBC001 was safe for the soil ecological environment.In addition,high-throughput sequencing results demonstrated that at 10 days post transplantation,NBC001 increased the abundances of Actinobacteria and Acidobacteria,but decreased the abundances of Bacteroidetes and Gemmatimonadetes at the phylum level.Meanwhile,the abundances of Phyllobacterium,Ralstonia and H16 were increased,while the abundances of Adhaeribacter,Gemmatimonas,Sphingomonas,Flavisolibacter\Nere suppressed by application of NBC001.However,at 90 days post transplantation,NBC001 only increased the abundances oi Aeromicrobium and RB41 whereas it decreased the abundance of H16.The results indicated that application of NBC001 increased the relative abundances of the beneficial microorganisms such as Actinobacteria,Acidobacteria,Aeromicrobium and Phyllobacterium in the soil.In summary,NBC001 is an eco-friendly biocontrol agent for H.glycines control.

关键词： Aspergillus niger NBC001 biocontrol Heterodera glycines high-throughput sequencing microbial community

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Potassium sulphate induces resistance of rice against the rootknot nematode Meloidogyne graminicola

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Journal of Integrative Agriculture 2022年第11期21卷 3263-3277页

作者： LIU Mao-Yan PENG De-liang SU Wen XIANG Chao JIAN Jin-zhuo ZHAO Jie peng Huan LIU Shi-ming KONG Ling-an DAI liang-ying HUANG Wen-kun LIU Jing Key Laboratory for Biology and Control of Plant Diseases and Insect Pests College of Plant ProtectionHunan Agricultural UniversityChangsha 410128P.R.China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China School of Agricultural Science Xichang UniversityXichang 615013P.R.China

Potassium(K),an important nutrient element,can improve the stress resistance/tolerance of crops.The application of K in resisting plant-parasitic nematodes shows that the K treatment can reduce the occurrence of nematode diseases and increase crop yield.However,data on K_(2)SO_(4)induced rice resistance against the root-knot nematode Meloidogyne graminicola are still lacking.In this work,K_(2)SO_(4)treatment reduced galls and nematodes in rice plants and delayed the development of nematodes.Rather than affecting the attractiveness of roots to nematodes and the morphological phenotype of giant cells at feeding sites,such an effect is achieved by rapidly priming hydrogen peroxide(H_(2)O_(2))accumulation and increasing callose deposition.Meanwhile,galls and nematodes in rice roots were more in the potassium channel OsAKT11 and transporter OsHAK5 gene-deficient plants than in wild-type,while the K_(2)SO_(4)-induced resistance showed weaker in the defective plants.In addition,during the process of nematode infection,the expression of jasmonic acid(JA)/ethylene(ET)/brassinolide(BR)signaling pathway-related genes and pathogenesis-related(PR)genes OsPR1 a/OsPR1 b was up-regulated in rice after K_(2)SO_(4)treatment.In conclusion,K_(2)SO_(4)induced rice resistance against M.graminicola.The mechanism of inducing resistance was to prime the basal defense and required the participation of the K^(+)channel and transporter in rice.These laid a foundation for further study on the mechanism of rice defense against nematodes and the rational use of potassium fertilizer on improving rice resistance against nematodes in the field.

关键词： rice Meloidogyne graminicola potassium sulphate induced resistance H_(2)O_(2) callose potassium channel and transporter

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A fragment of a 70-kDa Heterodera glycines heat shock protein(HgHSP70)interacts with soybean cyst nematode-resistant GmSHMT08

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Journal of Integrative Agriculture 2022年第10期21卷 2973-2983页

作者： LIU Zhi ZHANG Liu-ping ZHAO Jie JIAN Jin-zhuo peng Huan HUANG Wen-kun KONG Ling-an PENG De-liang LIU Shi-ming State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China MARA–CABI Joint Laboratory for Bio-safety Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China

Soybean cyst nematode(SCN)Heterodera glycines is considered as the major constraint to soybean production.Gm SHMT08 at Rhg4 locus on chromosome 08,encoding a serine hydroxylmethyltransferase,is a major gene underlying resistance against H.glycines in Peking-type soybeans.However,the molecular mechanism underpinning this resistance is less well characterized,and whether Gm SHMT08 could interact with proteins in H.glycines remains unclear.In this study,yeast two-hybrid screening was conducted using Gm SHMT08 as a bait protein,and a fragment of a 70-kDa heat shock protein(Hg HSP70)was screened from H.glycines that exhibited interaction with Gm SHMT08.This interaction was verified by both GST pull-down and bimolecular fluorescence complementation assays.Our finding reveals Hg HSP70 could be applied as a potential candidate gene for further exploring the mechanism on Gm SHMT08-mediated resistance against SCN H.glycines.

关键词： soybean cyst nematode Heterodera glycines GmSHMT08 HgHSP70 interaction resistance

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Mid-term results of hybrid arch repair for deBakey type I aortic dissection

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Chinese Medical Journal 2022年第4期135卷 493-495页

作者： liang Zhang Zhan peng Cuntao Yu Xiubin Yang Juntao Qiu de Wang department of Cardiac Surgery Beijing Anzhen HospitalBeijing Institute of HeartLung and Blood Vessel DiseaseCapital Medical UniversityBeijing 100029China The State Key Laboratory of Cardiovascular Disease Fuwai HospitalNational Center for Cardiovascular DiseaseChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100037China

To the Editor:Total aortic arch replacement(TAR)is a surgical procedure associated with significant perioperative risks,leading to a higher rate of mortality and neurologic complications.Ascending aortic or hemiarch replacement is associated with less complicated procedures,which can be used to effectively resect the proximal intimal tear and potentially improve the survival rates in these patients.[1]Hybrid aortic repair,combining openchest surgery and stent placement has been effectively used in patients with an aortic arch aneurysm.[2]Although a hybrid technique is often followed,only a limited number of studies with a small number of cases have investigated the application of this procedure for the treatment of type I aortic dissection.[1,2]This study aimed to evaluate the early and mid-term safety and efficacy of the hybrid technique in this cohort by analyzing a large sample size.

关键词： aortic dissection mortality

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Estimation of glacier ice storage in western China constrained by field ground-penetrating Radar surveys

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Advances in Climate Change Research 2022年第3期13卷 359-374页

作者： peng-Bin liang Li-de TIAN Institute of International Rivers and Eco-Security Yunnan UniversityKunming 650500China Yunnan Key Laboratory of International Rivers and Transboundary Eco-security Kunming 650091China

Recent decades have witnessed the accelerated retreat of alpine glaciers,which likely portends a decline in the glacial ice melt on which large downstream populations rely for freshwater.Thus,estimating water storage in alpine glaciers is critical for predicting the trend of glacier melting.This study compiled a rich set of ice thickness observations for glaciers of varying sizes in western China.We here presented a first-order assessment of the various errors involved in interpreting ice thickness from ground-penetrating radar(GPR)observations.An empirical Bayesian kriging(EBK)method was used for ice thickness interpolation and volume estimation.We then established a new volume-scaling law specific to western China glaciers based on these 36 volume-area pairs.And together with the Second Chinese Glacier Inventory(SCGI),we obtain a regional volume estimate of 4451±298 km^(3).By comparison,we believed that this result is the most reliable estimate of the total ice storage in western China and more reliably predicts gross glacier melting.However,our results show that the method of glacier division can strongly affect the total volume estimation,which previous studies ignored.This emphasizes the need for more surveyed glaciers data and more accurate glacier inventory to improve the evaluation of the climate impact on glacier melting water resources and to help ensure the future survival of these alpine glaciers.

关键词： Ground-penetrating radar Glacier thickness survey Glacier volume volume-area scaling law Distributed model Western China

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Molecular characterization and functional analysis of two new lysozyme genes from soybean cyst nematode(Heterodera glycines)

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Journal of Integrative Agriculture 2019年第12期18卷 2806-2813页

作者： WANG Ning peng Huan LIU Shi-ming HUANG Wen-kun Ricardo Holgado Jihong Liu-Clarke PENG De-liang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China NIBIO Norwegian Institute of Bioeconomy ResearchPb 115N0-1431 AsNorway

Soybean cyst nematode(SCN, Heterodera glycines(I.)) is one of the most important soil-borne pathogens for soybeans. In plant parasitic nematodes, including SCN, lysozyme plays important roles in the innate defense system. In this study, two new lysozyme genes(Hg-lys1 and Hg-lys2) from SCN were cloned and characterized. The in situ hybridization analyses indicated that the transcripts of both Hg-lys1 and Hg-lys2 accumulated in the intestine of SCN. The q RT-PCR analyses showed that both Hg-lys1 and Hg-lys2 were upregulated after SCN second stage juveniles(J2 s) were exposed to the Grampositive bacteria Bacillus thuringiensis, Bacillus subtilis or Staphylococcus aureus. Knockdown of the identified lysozyme genes by in vitro RNA interference caused a significant decrease in the survival rate of SCN. All of the obtained results indicate that lysozyme is very important in the defense system and survival of SCN.

关键词： soybean cyst nematode lysozyme Hg-lys1 Hg-lys2 innate defense against bacteria

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Assaying the potential of twenty-one legume plants in Medicago truncatula and M. sativa for candidate model plants for investigation the interactions with Heterodera glycines

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Journal of Integrative Agriculture 2016年第3期15卷 702-704页

作者： KONG Ling-an WU Du-qing HUANG Wen-kun peng Huan HE Wen-ting PENG De-liang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences

Soybean cyst nematode Heterodera glycines is one of the most serious soil-borne pathogens in soybean production. However, the researches were limited in China due to lack of an effective pathosystem. In this study, we screened 21 legume Medicago plants in both Medicago truncatula and Medicago sativa to obtain candidate model plants for establishing a new pathosystem for legume-H. glycines interactions. The nematode infection of tested plants was assayed with Race 3 and 4 respectively, which were two dominant H. glycines inbred races in China soybean producing areas. The results showed that the model legume plant M. truncatula A17 failed to allow Race 3 of H. glycines to complete its life cycle, in contrast, it provided the Race 4 population to form several cyst nematodes, however, the female index（FI） value was approximately 1.6. Three M. sativa cultivars, including Xunlu, Aergangjin and Junren, provided either Race 3 or 4 of H. glycines to develop into mature cysts with their FI value below 5 as well. Our results demonstrated that legume plants in both M. truncatula and M. sativa were not likely to be a model plant for H. glycines because of an extreme high resistance.

关键词： soybean cyst nematode alfalfa life cycle female index pathosystem

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Multiatom activation of single-atom electrocatalysts via remote coordination for ultrahigh-rate two-electron oxygen reduction

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Journal of Energy Chemistry 2023年第1期76卷 622-630,I0015页

作者： Xiaoqing Liu Rui Chen Wei peng Lichang Yin de'an Yang Feng Hou Liqun Wang Ji liang Key Laboratory of Advanced Ceramics and Machining Technology of Ministry of Education School of Materials Science and EngineeringTianjin UniversityTianjin 300072China Shenyang National Laboratory for Materials Science Institute of Metal ResearchChinese Academy of SciencesShenyang 110016LiaoningChina Applied Physics department College of Physics and Materials ScienceTianjin Normal UniversityTianjin 300387China

Electrocatalytic oxygen reduction via a two-electron pathway(2e^(-)-ORR)is a promising and eco-friendly route for producing hydrogen peroxide(H_(2)O_(2)).Single-atom catalysts(SACs)typically show excellent selectivity towards 2e^(-)-ORR due to their unique electronic structures and geometrical configurations.The very low density of single-atom active centers,however,often leads to unsatisfactory H_(2)O_(2)yield rate,significantly inhibiting their practical feasibility.Addressing this,we herein introduce fluorine as a secondary doping element into conventional SACs,which does not directly coordinate with the singleatom metal centers but synergize with them in a remote manner.This strategy effectively activates the surrounding carbon atoms and converts them into highly active sites for 2e^(-)-ORR.Consequently,a record-high H_(2)O_(2)yield rate up to 27 mol g^(-1)h^(-1)has been achieved on the Mo–F–C catalyst,with high Faradaic efficiency of 90%.density functional theory calculations further confirm the very kinetically facile 2e^(-)-ORR over these additional active sites and the superiority of Mo as the single-atom center to others.This strategy thus not only provides a high-performance electrocatalyst for 2e^(-)-ORR but also should shed light on new strategies to significantly increase the active centers number of SACs.

关键词： Hydrogen peroxide Oxygen reduction reaction Two-electron pathway Remote coordination Electrocatalysis

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