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Nested RT-PCR method for the detection of European avian-like H1 swine influenza A virus

Journal of Integrative Agriculture 2016年第5期15卷 1095-1102页

作者： WEI Yan-di pei xing-yao ZHANG Yuan YU Chen-fang SUN Hong-lei LIU Jin-hua PU Juan Key Laboratory of Animal Epidemiology and Zoonosis Ministry of Agriculture/State Key Laboratory of Agrobiotechnology/College of Veterinary Medicine China Agricultural University Beijing 100193 P.R. China China Institute of Veterinary Drug Control Beijing 100086 P.R. China

Swine influenza A virus（swine IAV） circulates worldwide in pigs and poses a serious public health threat, as evidenced by the 2009 H1N1 influenza pandemic. Among multiple subtypes/lineages of swine influenza A viruses, European avian-like（EA） H1N1 swine IAV has been dominant since 2005 in China and caused infections in humans in 2010. Highly sensitive and specific methods of detection are required to differentiate EA H1N1 swine IAVs from viruses belonging to other lineages and subtypes. In this study, a nested reverse transcription（RT）-PCR assay was developed to detect EA H1 swine IAVs. Two primer sets（outer and inner） were designed specifically to target the viral hemagglutinin genes. Specific PCR products were obtained from all tested EA H1N1 swine IAV isolates, but not from other lineages of H1 swine IAVs, other subtypes of swine IAVs, or other infectious swine viruses. The sensitivity of the nested RT-PCR was improved to 1 plaque forming unit（PFU） m L^（-1） which was over 10~4 PFU m L^（-1） for a previously established multiplex RT-PCR method. The nested RT-PCR results obtained from screening 365 clinical samples were consistent with those obtained using conventional virus isolation methods combined with sequencing. Thus, the nested RT-PCR assay reported herein is more sensitive and suitable for the diagnosis of clinical infections and surveillance of EA H1 swine IAVs in pigs and humans.

关键词： nested RT-PCR swine influenza A virus European avian-like H1 HA gene molecular diagnosis

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A duplex RT-PCR assay for detection of H9 subtype avian influenza viruses and infectious bronchitis viruses

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Journal of Integrative Agriculture 2016年第9期15卷 2105-2113页

作者： WEI Yan-di GAO Wei-hua SUN Hong-lei YU Chen-fang pei xing-yao SUN Yi-peng LIU Jin-hua PU Juan Key Laboratory of Animal Epidemiology and Zoonosis Ministry of Agriculture/College of Veterinary Medicine/State Key Laboratory of Agrobiotechnology China Agricultural University

H9 s ubtype avian influenza virus（AIV） and infectious bronchitis virus（IBV） are major pathogens circulating in poultry and have resulted in great economic losses due to respiratory disease and reduced egg production. As similar symptoms are elicited by the two pathogens, it is difficult for their differential diagnosis. So far, no reverse transcription-polymerase chain reaction（RT-PCR） assay has been found to differentiate between H9 AIV and IBV in one reaction. Therefore, developing a sensitive and specific method is of importance to simultaneously detect and differentiate H9 AIV and IBV. In this study, a duplex RT-PCR（d RT-PCR） was established. Two primer sets target the hemagglutinin（HA） gene of H9 AIV and the nucleocapsid（N） gene of IBV, respectively. Spec ific PCR products were obtained from all tested H9 AIVs and IBVs belonging to the major clades circulating in China, but not from AIVs of other subtypes or other infectious avian viruses. The sensitivity of the d RT-PCR assay corresponding to H9 AIV, IBV and mixture of H9 AIV and IBV were at a concentration of 1×10^1, 1.5×10^1 and 1.5×10^1 50% egg infective doses（EID_（50）） m L^–1, respectively. The concordance rates between the d RT-PCR and virus isolation were 99.1 and 98.2%, respectively, for detection of samples from H9N2 AIV or IBV infected chickens, while the concordance rate was 99.1% for detection of samples from H9N2 AIV and IBV co-infected chickens. Thus, the d RT-PCR assay reported herein is specific and sensitive, and suitable for the differential diagnosis of clinical infections and survei llance of H9 AIVs and IBVs.

关键词： avian influenza viruses H9 subtype infectious bronchitis viruses duplex RT-PCR

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氧化锌纳米粒子的神经毒性研究进展

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中国药理学与毒理学杂志 2021年第8期35卷 632-640页

作者：裴星瑶李道稳汤树生中国农业大学动物医学院北京100193 天津农学院动物科学与动物医学学院天津市农业动物繁育与健康养殖重点实验室天津300384

氧化锌纳米粒子(ZnO NP)作为一种重要的纳米材料广泛应用于各个领域,人类和动物易于通过皮肤、呼吸道、消化道等途径接触。ZnO NP可以纳米颗粒原型或代谢转化后的离子形式到达大脑,造成脑内Zn失衡,引起神经毒性。但目前ZnO NP的神经毒... 详细信息

氧化锌纳米粒子(ZnO NP)作为一种重要的纳米材料广泛应用于各个领域,人类和动物易于通过皮肤、呼吸道、消化道等途径接触。ZnO NP可以纳米颗粒原型或代谢转化后的离子形式到达大脑,造成脑内Zn失衡,引起神经毒性。但目前ZnO NP的神经毒性作用机制并未得到全面认知。中枢神经系统中ZnO NP通过血脑屏障途径、感觉神经易位途径以及微生物-肠道-大脑轴途径介导Zn在中枢神经系统的聚积。ZnO NP能够通过神经毒性诱导神经行为障碍和脑组织损伤,本文对ZnO NP引发的神经信号传导障碍、线粒体氧化应激、神经炎症和细胞凋亡等神经毒性作用机制进行综述,为该纳米粒子的合理应用和风险评估提供依据。

关键词：氧化锌纳米颗粒脑神经毒性风险评估

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An Unprecedented Efficiency with Approaching 21%Enabled by Additive‑Assisted Layer‑by‑Layer Processing in Organic Solar Cells

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Nano-Micro Letters 2025年第2期17卷 372-375页

作者： Shuai Xu Youdi Zhang Yanna Sun pei Cheng Zhaoyang yao Ning Li Long Ye Lijian Zuo Ke Gao Key Laboratory of Advanced Green Functional Materials College of ChemistryChangchun Normal UniversityChangchun 130032People’s Republic of China Shandong Provincial Key Laboratory for Science of Material Creation and Energy Conversion School of Chemistry and Chemical EngineeringShandong UniversityQingdao 266237People’s Republic of China College of Polymer Science and Engineering Sichuan UniversityChengdu 610065People’s Republic of China State Key Laboratory and Institute of Elemento‑Organic Chemistry Centre of Nanoscale Science and Technology and Key Laboratory of Functional Polymer MaterialsRenewable Energy Conversion and Storage Center(RECAST)College of ChemistryNankai UniversityTianjin 300071People’s Republic of China Institute of Polymer Optoelectronic Materials and Devices State Key Laboratory of Luminescent Materials and Devices South China University of TechnologyGuangzhou 510640People’s Republic of China Tianjin Key Laboratory of Molecular Optoelectronic Sciences School of Materials Science and EngineeringTianjin UniversityTianjin 300350People’s Republic of China State Key Laboratory of Silicon Materials MOE Key Laboratory of Macromolecular Synthesis and FunctionalizationDepartment of Polymer Science and EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China

Recently published in Joule,Feng Liu and colleagues from Shanghai Jiaotong University reported a record-breaking 20.8%power conversion efficiency in organic solar cells(OSCs)with an interpenetrating fibril network active layer morphology,featuring a bulk p-in structure and proper vertical segregation achieved through additive-assisted layer-by-layer *** optimized hierarchical gradient fibrillar morphology and optical management synergistically facilitates exciton diffusion,reduces recombination losses,and enhances light capture *** approach not only offers a solution to achieving high-efficiency devices but also demonstrates the potential for commercial applications of OSCs.

关键词： Organic solar cells Additive-assisted layer-by-layer processing Three-dimensional fibril morphology Bulk p-i-n structure Optical management

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快速检测牛奶中泰乐菌素残留的荧光微球免疫层析方法的建立

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中国兽医杂志 2016年第10期52卷 71-73,I0002页

作者：王旗管笛武会娟裴星瑶彭涛江海洋中国农业大学动物医学院北京海淀100193 北京市理化分析测试中心北京海淀100089 北京市基因测序与功能分析工程技术研究中心北京海淀100094

本研究旨在研制一种快速检测牛奶中泰乐菌素残留的荧光微球免疫层析试纸条。将包被抗原(0.80 mg/mL)和羊抗鼠IgG(1.01 mg/mL)喷涂在硝酸纤维素膜上,分别作为检测线和质检线。对制备荧光微球抗体免疫复合物(免疫探针)的工艺参数做了一系... 详细信息

本研究旨在研制一种快速检测牛奶中泰乐菌素残留的荧光微球免疫层析试纸条。将包被抗原(0.80 mg/mL)和羊抗鼠IgG(1.01 mg/mL)喷涂在硝酸纤维素膜上,分别作为检测线和质检线。对制备荧光微球抗体免疫复合物(免疫探针)的工艺参数做了一系列的优化,同时比较了不同标记方法对标记效率及稳定性的影响。结果表明,在pH值为6.0的最佳缓冲体系条件下,催化剂碳化二亚胺(EDC)用量为5μg,抗体100倍稀释添加20μL,检测时间低于25 min,检测限为25μg/L。本试验研制的荧光微球免疫层析试纸条具有简便快速、特异性良好、样本无需前处理等特点,可用于基层奶站和现场快速筛选。

关键词：荧光微球泰乐菌素免疫层析方法试纸条

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“互联网+”时代下的高校地质教学改革与探索

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中国地质教育 2017年第2期26卷 29-31页

作者：楼仁兴裴尧王晰李竑逵吉林大学应用技术学院吉林长春130012 长春市晨光学校吉林长春130012

移动互联网已经全面渗透到校园的每一个角落,尤其是大学校园网络的发展,使大学生上网意识普遍得到增强,"互联网+"时代已经到来。在"大学生创新创业训练计划"的激励下,学生结合专业课的特点,自主开发了"地学导航"手机APP,并尝试在课堂教... 详细信息

移动互联网已经全面渗透到校园的每一个角落,尤其是大学校园网络的发展,使大学生上网意识普遍得到增强,"互联网+"时代已经到来。在"大学生创新创业训练计划"的激励下,学生结合专业课的特点,自主开发了"地学导航"手机APP,并尝试在课堂教学中推广应用,取得了较明显的教学效果。采用适合学生的教学手段和方法,能激发学生对课程的学习兴趣,增强学生对课程价值的认同度,从而构建移动互联网下教与学的新型关系和模式。

关键词：移动互联网互联网+ 校园网络大学生创新创业教学改革

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Depression mechanism of sulfite ions on sphalerite and Pb^(2+)activated sphalerite in the flotation separation of galena from sphalerite

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 335-345页

作者： Feng Zhang Chenyang Zhang Linlin Wu Wei Sun Hongliang Zhang Jianhua Chen Yong pei Songjiang Li Engineering Research Center of Ministry of Education for Carbon Emission Reduction in Metal Resource Exploitation and Utilization Hunan International Joint Research Center for Efficient and Clean Utilization of Critical Metal Mineral ResourcesSchool of Minerals Processing and BioengineeringCentral South UniversityChangsha 410083China State Key Laboratory of Mineral Processing Beijing General Research Institute of Mining&MetallurgyBeijing 100160China Hunan Provincial Key Laboratory of Micro&Nano Materials Interfaces Science College of Chemistry and Chemical EngineeringCentral South UniversityChangsha 410083China School of Resources Environment and MaterialsGuangxi UniversityNanning 530004China Department of Chemistry Xiangtan UniversityXiangtan 411105China China Railway Resource Group Co.Ltd Beijing 102300China

The depression mechanism of sulfite ions on sphalerite and Pb^(2+)activated sphalerite in the flotation separation of galena from sphalerite still lacked in-depth ***,the depression mechanism of sulfite ions on sphalerite and Pb^(2+)activated sphalerite in the flotation separation of galena from sphalerite was further systematically investigated with experiments and density functional theory(DFT)*** X-ray photoelectric spectroscopy(XPS)results,DFT calculation results,and frontier molecular orbital analysis indicated that sulfite ions were difficult to be adsorbed on sphalerite surface,suggesting that sulfite ions achieved depression effects on sphalerite through other non-adsorption ***,the oxygen content in the surface of sphalerite treated with sulfite ions in creased,which enhanced the hydrophilicity of the sphalerite and further increased the difference in hydrophilicity between sphalerite and ***,sulfite ions were chelated with lead ions to form PbSO_(3)in *** hydrophilic PbSO_(3)was more easily adsorbed on sphalerite than *** interaction between sulfite ions and lead ions could effectively inhibit the activation of *** addition the UV spectrum showed that after adding sulfite ions,the peak of perxanthate in the sphalerite treated xanthate solution was significantly stronger than that in the galena with xanthate solution,indicating that xanthate interacted more readily with sulfite ions and oxygen mo lecules within the sphalerite system,leading to the formation of ***,sulfite ions hardly depressed the flotation of ga lena and could promote the flotation of galena to some *** study deepened the understanding of the depression mechanism o sulfite ions on sphalerite and Pb^(2+)activated sphalerite.

关键词： sphalerite galena sulfite ion density functional theory depression mechanism

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荧光微球免疫层析法快速检测鸡饲料中喹乙醇的含量

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中国兽医杂志 2016年第9期52卷 83-85,90页

作者：裴星瑶王旗谢洁谢三磊彭涛李向梅江海洋中国农业大学动物医学院北京海淀100193 中国检验检疫科学研究院北京朝阳100176

为更灵敏地进行全价鸡饲料中喹乙醇（OLA）含量的快速检测,本研究将新型标记物荧光微球与喹乙醇单克隆抗体偶联,以该复合物为检测试剂制备荧光微球免疫层析试纸条,在肉眼检测的同时,将该装置与荧光微球定量侧向层析读数仪联合使用,可进... 详细信息

为更灵敏地进行全价鸡饲料中喹乙醇（OLA）含量的快速检测,本研究将新型标记物荧光微球与喹乙醇单克隆抗体偶联,以该复合物为检测试剂制备荧光微球免疫层析试纸条,在肉眼检测的同时,将该装置与荧光微球定量侧向层析读数仪联合使用,可进行全价鸡饲料中喹乙醇含量的定量快速检测。研究结果证明,饲料中喹乙醇的定性和定量检测限分别为100μg/kg和0.51μg/kg,IC_（50）值为1.89 ng/m L,定量检测回收率为78.71%-90.83%。该试纸条特异性强、准确性高,保存期长。定量检测只需18 min左右,肉眼检测需38 min左右,与相同抗原和抗体制备的胶体金试纸条相比,该方法可大大提高灵敏度。

关键词：喹乙醇荧光微球免疫层析鸡饲料

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锌诱导神经毒性损伤机制研究进展

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中国兽医杂志 2023年第4期59卷 103-108页

作者：刘鑫宇裴星瑶李存汤树生张宇航崔云昊魏怡李道稳天津农学院动物科学与动物医学学院、天津市农业动物繁育与健康养殖重点实验室天津西青300384 中国农业大学动物医学院北京海淀100193

锌是存在于动物和人类中枢神经系统(CNS)第二丰富的微量元素,是维持脑功能所必需的调控因子,因此在动物饲料和人类食物中必不可少。然而,锌也是一种潜在有毒金属,其平衡失调与神经疾病的发病机制密切相关。过量的锌会诱导神经元氧化应... 详细信息

锌是存在于动物和人类中枢神经系统(CNS)第二丰富的微量元素,是维持脑功能所必需的调控因子,因此在动物饲料和人类食物中必不可少。然而,锌也是一种潜在有毒金属,其平衡失调与神经疾病的发病机制密切相关。过量的锌会诱导神经元氧化应激、线粒体损伤和炎症反应,最终通过凋亡和自噬诱发神经元死亡。本文对锌的神经毒性及其作用机制的研究进展进行综述,以期为锌稳态失调引起疾病的深入研究提供参考。

关键词：锌神经毒性机制

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THE INTRUSION AND INFLUENCES OF INTRASEASONAL LONG ROSSBY WAVES IN THE EAST CHINA SEA

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Journal of Hydrodynamics 2004年第5期16卷 621-632页

作者： LINXiao-pei WUDe-xing LANJian InstituteofphysicalOceanographyandPhysicalOceanographyLaboratory OceanUniversityofChinaQingdao266003China

The analysis of sea level data from tide stations and ocean generalcirculation model (OGCM) shows that the intraseasonal long Rossby wave results in the intraseasonalvariations of Kuroshio axis and transport in the upper reaches of the Kuroshio in the East China Sea(ECS). Due to the transport modulation induced by Ryukyu Islands, the intraseasonal variation isvery weak in the lower reaches of the Kuroshio in the ECS. A two-layer model reveals the relationamong Kuroshio frontal unstable wave with topography, transport and the axis position of theKuroshio, from which we can infer that the intrusion of intraseasonal long Rossby wave may stimulatethe Kuroshio frontal unstable wave in the upper reaches of the Kuroshio in the ECS. The altimeterdata also shows that the sea level anomaly resulting from intraseasonal long Rossby wave could passthe channel between Taiwan and Ryukyu Islands and propagate northeastward in accord with theKuroshio frontal unstable wave. However, the sea level anomalies passing through the gaps of RyukyuIslands are relative weak and have negligible effects on the Kuroshio variation.

关键词： kuroshio intraseasonal long rossby wave unstable wave

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