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Fabrication and characterization of 3D scaffold using 3D plotting system

Chinese Science Bulletin 2010年第1期55卷 94-98页

作者： LEE Jun-Hee park Su-A park koeun KIM Jae-Hyun KIM Kyung-Shik LEE Jihye Kim WanDoo Bio-Mechatronics Team Division of Nano-Machinery Korea Institute of Machinery and Materials (KIMM) Nano-Mechanics Team Division of Nano-Machinery Korea Institute of Machinery and Materials (KIMM) Nano-Mechatronics Team Division of Nano-Machinery Korea Institute of Machinery and Materials (KIMM)

In this paper, we design and fabricate a 3D scaffold using rapid prototyping (RP) technology for tissue engineering. The scaffold should have a three-dimensional interconnected pore network. We fabricate a polycaprolactone (PCL) scaffold with interconnecting pores and uniform porosity for cell ingrowth using a 3D plotting system. In order to keep the three dimensional shape under mechanical loading while implanted, we design an oscillating nozzle system to increase elastic modulus and yield strength of PCL strand. We characterize the influence of pore geometry, compressive modulus of the scaffold, elastic modulus and yield strength of the strand using SEM, dynamical mechanical analysis (DMA) and Nano-UTM. Finally the cell responses on scaffolds are observed.

关键词：三维形状绘图系统表征支架制作动态机械分析弹性模量细胞反应

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Reactive oxygen species-induced activation of Yes-associated protein-1 through the c-Myc pathway is a therapeutic target in hepatocellular carcinoma

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World Journal of Gastroenterology 2020年第42期26卷 6599-6613页

作者： Yuri Cho Min Ji park koeun Kim Sun Woong Kim Wonjin Kim Sooyeon Oh Joo Ho Lee Department of Internal Medicine CHA Gangnam Medical CenterCHA University School of MedicineSeoul 06135South Korea Department of Internal Medicine Chaum Life CenterCHA University School of MedicineSeoul 06062South Korea Department of Internal Medicine CHA Bundang Medical CenterCHA University School of MedicineSeongnam 13496Bundang guSouth Korea

BACKGROUND The Hippo signaling pathway regulates organ size by regulating cell proliferation and apoptosis with terminal effectors including Yes-associated protein-1(YAP-1).Dysregulation in Hippo pathway has been proposed as one of the therapeutic targets in *** levels of reactive oxygen species(ROS)increase during the progression from early to advanced hepatocellular carcinoma(HCC).AIM To study the activation of YAP-1 by ROS-induced damage in HCC and the involved signaling *** The expression of YAP-1 in HCC cells(Huh-7,HepG2,and SNU-761)was quantified using real-time polymerase chain reaction and *** HCC cells were treated with H2O2,which is a major component of ROS in living organisms,and with either YAP-1 small interfering RNA(siRNA)or control *** investigate the role of YAP-1 in HCC cells under oxidative stress,MTS assays were *** was performed to evaluate the signaling pathway responsible for the activation of ***-eight surgically resected frozen HCC tissue samples and 88 nontumor liver tissue samples were used for gene expression *** H2O2 treatment increased the mRNA and protein expression of YAP-1 in HCC cells(Huh-7,HepG2,and SNU-761).Suppression of YAP-1 using siRNA transfection resulted in a significant decrease in tumor proliferation during H2O2 treatment both in vitro and in vivo(both P<0.05).The oncogenic action of YAP-1 occurred via the activation of the c-Myc pathway,leading to the upregulation of components of the unfolded protein response(UPR),including 78-kDa glucoseregulated protein and activating transcription factor-6(ATF-6).The YAP-1 mRNA levels in human HCC tissues were upregulated by 2.6-fold compared with those in nontumor tissues(P<0.05)and were positively correlated with the ATF-6 Levels(Pearson’s coefficient=0.299;P<0.05).CONCLUSION This study shows a novel connection between YAP-1 and the UPR through the c-Myc pathway during oxidative stress

关键词： Hepatocellular carcinoma Yes-associated protein-1 C-Myc Reactive oxygen species Unfolded protein response Activating transcription factor-6

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