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陆地棉离层基因GhBOP1的克隆及其功能的初步分析

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华北农学报 2015年第3期30卷 14-19页

作者：常璟晋昕庞金环司怀军张宁吴家和甘肃农业大学生命科学技术学院甘肃兰州730070 中国科学院微生物研究所北京100101

为防止棉铃脱落提高结铃性,进而提高棉花产量,从陆地棉中分离出一个参与离层分化调控的相关基因GhBOP1。通过生物信息学分析,表明GhBOP1蛋白序列含有保守性强的BTB和ANK结构域;进化树分析显示该蛋白氨基酸序列与许多植物的同源蛋白相似... 详细信息

为防止棉铃脱落提高结铃性,进而提高棉花产量,从陆地棉中分离出一个参与离层分化调控的相关基因GhBOP1。通过生物信息学分析,表明GhBOP1蛋白序列含有保守性强的BTB和ANK结构域;进化树分析显示该蛋白氨基酸序列与许多植物的同源蛋白相似程度很高,说明该类蛋白进化较慢。利用qRT-PCR进行组织特异性表达分析的结果表明,GhBOP1基因在根、茎、叶和花等组织器官中均有少量表达,但在离层中为优势表达,表达量为叶片表达水平的20倍。利用病毒诱导基因沉默(VIGS)技术干涉棉花GhBOP1基因的表达,同时进行低盐胁迫处理分析,对照植株叶片出现大量地脱落,而GhBOP1基因沉默植株叶片生长正常。总之GhBOP1基因在离层中优势表达,并对离层的形成可能具有调控功能。

关键词：棉花离层 GhBOP1基因病毒诱导基因沉默

水稻丙酮酸激酶基因OsPK1的过表达转基因株系的获得与鉴定

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华北农学报 2012年第2期27卷 1-5页

作者：张艳庞金环吴家和何朝族清华大学生命科学学院北京100084 清华大学深圳研究生院广东深圳518055 中国科学院微生物研究所植物基因组国家重点实验室北京100101 海南大学海南省热带生物资源可持续利用重点实验室海南海口570228

利用农杆菌介导的转化方法研究OsPK1过表达对水稻的影响。将CaMV 35S启动子驱动的OsPK1的全长CDS序列导入到日本晴基因组中。选择3个过表达转基因系作为代表进行鉴定和分析。结果显示,过表达植株在株高、分蘖数、穗长和千粒重四方面接... 详细信息

利用农杆菌介导的转化方法研究OsPK1过表达对水稻的影响。将CaMV 35S启动子驱动的OsPK1的全长CDS序列导入到日本晴基因组中。选择3个过表达转基因系作为代表进行鉴定和分析。结果显示,过表达植株在株高、分蘖数、穗长和千粒重四方面接近野生型水稻,结实率略下降,但每穗饱满种子数明显增加。通过定量RT-PCR分析,发现有4个代谢酶在过表达植株嫩叶中表达存在着上调或下调。用GC-MS方法检测糖含量,显示OsPK1过表达株系中葡萄糖、果糖、半乳糖和蔗糖的含量与野生型差异不大。总之,对OsPK1过表达株系的鉴定,有助于更好了解OsPK1的功能,并且每穗饱满种子数明显增加具潜在的应用价值。

关键词： OsPK1 过表达丙酮酸激酶水稻

CoP@SiO2nanoreactors: A core-shell structure for efficient electrocatalytic oxygen evolution reaction

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Chinese Chemical Letters 2020年第9期31卷 2300-2304页

作者： Ling jin huan pang School of Chemistry and Chemical Engineering Yangzhou UniversityYangzhou 225009China

Metallic phosphides as a crucial class of metal-like compounds show high electric conductivity and electrochemical *** is of significant benefit to understanding the relationship between the electrocatalytic performance and phosphating degree of *** this work,using Co3O4@SiO2 as precursor,core-shell structured CoP@SiO2 nanoreactors with outstanding oxygen evolution reaction performance were synthesized through a facile calcination *** electrocatalytic performance of CoP@SiO2 modified electrode that treated with 500 mg NaH2PO2 was greatly *** obtained product displays a low overpotential of 280 mV at a current density of 10 mA/cm2 and a Tafel value 89 mV/dec in alkaline *** easy available CoP@SiO2 with outstanding catalytic performance and stability possesses huge potential in future electrochemical applications.

关键词： Core-shell structure Phosphating Nanoreactors Oxygen evolution reaction Silicon shell

56种中药提取物对棉花黄萎病的防治效果研究

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生物技术通报 2018年第2期34卷 128-134页

作者：姜岚庞金环肖伟烈张国丽刘俊杨超中国科学院微生物研究所北京100101 云南大学教育部和云南省自然资源药物化学重点实验室昆明650091 新疆农垦科学院石河子832000

为获得既能有效抑制棉花黄萎病致病菌(大丽轮枝菌)菌丝生长又可以提高棉花黄萎病防治效果的中药提取物,对56种中药提取物的抑菌活性及防治效果进行了筛选和鉴定。生长速率法测定结果显示,56种中药提取物中有26种在质量浓度为10 g/L时对... 详细信息

为获得既能有效抑制棉花黄萎病致病菌(大丽轮枝菌)菌丝生长又可以提高棉花黄萎病防治效果的中药提取物,对56种中药提取物的抑菌活性及防治效果进行了筛选和鉴定。生长速率法测定结果显示,56种中药提取物中有26种在质量浓度为10 g/L时对大丽轮枝菌的菌丝生长抑制率可达到50%以上,主要集中在五味子、铁箍散、华盖木和黄苞大戟等9种植物提取物中。进一步用这些中药提取物处理棉花植株,统计病情指数和防治效果发现,黑龙江产五味子(北五味子)的氯仿∶丙酮=3∶2(V/V)段提取物对棉花黄萎病有显著的防治效果。茎切片观察发现,该提取物可以有效抑制大丽轮枝菌引起的维管束褐化症状,且棉花整株表现为黄萎病落叶症状得到缓解。上述研究表明,黑龙江产五味子的氯仿∶丙酮=3∶2段提取物不仅可以有效抑制大丽轮枝菌的菌丝生长,而且可以提高棉花黄萎病的防治效果,降低维管束褐化并缓解发病程度。

关键词：中药提取物大丽轮枝菌棉花黄萎病防治效果

Nanoreactors derived from silica-protection-assisted metal-organic framework

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Chinese Chemical Letters 2020年第9期31卷 2207-2210页

作者： Ling jin Xiaxia Li Chunsen Liu huan pang School of Chemistry and Chemical Engineering Guangling CollegeYangzhou UniversityYangzhou 225009China Henan Provincial Key Laboratory of Surface&Interface Science Zhengzhou University of Light IndustryZhengzhou 450002China

The construction of highly stable and regular nanoreactors is a major *** this work,we use a facile template protection method to obtain ZIF-67@SiO2(JS) and to encapsulate metal oxide nanoparticles(Co3O4) into nanoreactors(SiO2).ZIF-67 crystals provide a cobalt species;SiO2 was first used as a protective layer of ZIF-67 and then as a nanoreactor for metastable metal oxide *** this basis,Co3O4@SiO2 with dodecahedron morphology were synthesized by calcining JS at different tempe ratures,followed by a hydrothermal reaction to obtain Co3(OH)***,CoSx and CoP-SiO2 were fabricated through sulfuration and *** results in this work show that nanoreactors derived from metal-organic frameworks(MOFs) with a rational structure have broad development prospects.

关键词： Core-shell structure Metal oxide nanoparticles Co3(OH)4Si2O5 Nanoreactors Silicon shell

Influence of(Mg_(1/3)Nb_(2/3))complex substitutions on crystal structures and microwave dielectric properties of Li2TiO3 ceramics with extreme low loss

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Journal of Materiomics 2018年第4期4卷 368-382页

作者： huan-huan Guo Di Zhou Li-Xia pang jin-Zhan Su Electronic Materials Research Laboratory Key Laboratory of the Ministry of Education&International Center for Dielectric ResearchSchool of Electronic and Information EngineeringXi'an Jiaotong UniversityXi'an710049China Micro-optoelectronic Systems Laboratories Xi'an Technological UniversityXi'an710032ShaanxiChina International Research Centre for Renewable Energy State Key Laboratory of Multiphase Flow in Power EngineeringXi'an Jiaotong UniversityXi'anShaanxi710049China

A systematic investigation of the Li2Ti1-x(Mg_(1/3)Nb_(2/3))xO_(3)(0.1≤x≤0.35)solid solutions synthesized by traditional solid-state reaction method is reported in this *** the composition range of 0.1≤x≤0.25,a monoclinic rock salt structured solid solution was *** x increased to 0.3,a phase transition from monoclinic to cubic phase,along with an order-disorder phase transition,was *** x increased from 0.1 to 0.35,the microwave permittivity(εr)and temperature coefficient of resonant frequency(TCF)of the Li2Ti1-x(Mg_(1/3)Nb_(2/3))xO_(3)ceramics decreased linearly from 21.0 to 18.6,+27.1 to-19.4 ppm/℃,*** Li_(2)Ti_(0.75)(Mg_(1/3)Nb_(2/3))0.25O_(3)ceramic sintered at 1170℃shows high performance of microwave dielectric properties with aεr~19.6,a Qf(Q=quality factor=1/dielectric loss;f=resonant frequency)~109,770 GHz(at 7.7 GHz)and a near zero TCF~t 1.2 ppm/℃.Moreover,the burying sintering process can reduce the volatilization of lithium so that the porosity of Li_(2)Ti_(0.75)(Mg_(1/3)Nb_(2/3))0.25O_(3)ceramic was reduced effectively,which made Li_(2)Ti_(0.75)(Mg_(1/3)Nb_(2/3))0.25O_(3)ceramic promising for future *** area electron diffraction patterns,high-resolution transmission electron microscopy,Raman and far-infrared spectra were employed to study the relation between crystal structure and microwave dielectric properties in detail.

关键词： Microwave dielectric properties Phase evolution Crystal structure High quality

Rapid construction of highly-dispersed cobalt nanoclusters embedded in hollow cubic carbon walls as an effective polysulfide promoter in high-energy lithium-sulfur batteries

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Nano Research 2022年第6期15卷 5105-5113页

作者： Lin Sun Yanxiu Liu Kaiqiang Zhang Feng Cheng Ruiyu Jiang Yangqing Liu jing Zhu Zhong jin huan pang Key Laboratory for Advanced Technology in Environmental Protection of Jiangsu Province School of Chemistry and Chemical EngineeringYancheng Institute of TechnologyYancheng 224051China MOE Key Laboratory of Mesoscopic Chemistry MOE Key Laboratory of High Performance Polymer Materials and TechnologyJiangsu Key laboratory of Advanced Organic MaterialsSchool of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China School of Chemistry and Chemical Engineering Yangzhou UniversityYangzhou 225009China

The ultrahigh specific energy density and low cost of lithium-sulfur batteries are suitable for the next generation of energy ***,the shuttle issue and sluggish conversion kinetics of polysulfides remain *** metal nanoclusters with strong polarity in conductive porous carbon is an effective strategy for tackling such knotty ***,we design and synthesize hollow cubic carbon embedded with highly dispersed cobalt nanoclusters as an effective sulfur reservoir for lithium sulfur *** large cavity structure and well-dispersed cobalt nanoclusters,with uniform sizes near 11 nm,enable the hosting structure to hold the high sulfur loading,70%capacity retention after 500 cycles at 2 C with a high sulfur loading of 6.5 mg·cm^(−2),effective stress release,accelerated polysulfide conversion,superior rate performance,strong physical confinement and chemical absorption *** density functional theoretical calculations demonstrate that the welldispersed cobalt nanoclusters in the hosting structure play a critical electrocatalytic role in boosting the capability of absorbing and converting polysulfides.

关键词： lithium-sulfur battery electrocatalysis Co nanocluster hollow carbon shuttle effect

Synthesis and luminescence properties of Ca_2SiO_4-based red phosphors with Sm^(3+) doping for white LEDs

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International Journal of Minerals,Metallurgy and Materials 2012年第7期19卷 663-667页

作者： jin-jun Cai huan-huan Pan Yi Wang School of Applied Physics and Materials Wuyi University Jiangmen 529020 China

Sm3＋-activated Ca2SiO4 red phosphors were prepared by the conventional high-temperature solid-state reaction method, and the effects of sodium （Na＋） and samarium （Sm3＋） ions doping concentrations on their crystal structure and luminescent properties were investigated by X-ray diffraction （XRD） and fluorescent spectrofluorometer. XRD patterns demonstrate that a well-crystalline structure forms in the phosphors when they are treated by calcination at 1200~C for 4 h, and the excitation spectra exhibit good absorption in the range between 350 and 420 nm. Under the irradiation of 405 nm near-ultraviolet （NUV） light, the spectra of the phosphors show a main emission peak at 601 nm attributed to the 4G5/2→6H7/2 transition of Sm3＋ ions, and its intensity is greatly influenced by the concentrations of Sm3＋ and Na2CO3. When the concentrations of Sm3＋ ions and Na2CO3 are 2mol% and 6mol%, respectively, the optimal emission intensity can be obtained. From strong absorption in the near ultraviolet zone, the Na0.06Sm0.02Ca1.92SiO4 phosphor is a promising red-emitting phosphor for white light emitting diodes （W-LEDs）.

关键词： light emitting diodes phosphors photoluminescence doping samarium calcium silicate

Investigations on the Interactions between Li-TFSI and Glass Fibers in the Ternary PP/GF/Li-TFSI Composites

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Chinese Journal of Polymer Science 2018年第1期36卷 113-118页

作者： Sen-Lin Gu huan-huan Liu huan Cao Claude Mercier Yong-jin Li College of Material Chemistry and Chemical EngineeringHangzhou Normal UniversityHangzhou 310036China Solvay Co.Ltd shanghai 201100China

The polypropylene/glass fiber（PP/GF） composites with excellent antistatic performance and improved mechanical properties have been reported by incorporation of a very small amount of the organic salt, lithium bis（trifluoromethanesulfonyl）imide（Li-TFSI）, into the PP/GF composites. It was considered that GF could play the role as the pathways for the movements of ions in the ternary composites. In this work, the interactions between Li-TFSI and glass fiber and the effects of such interactions on the physical properties of the composites have been systematically investigated. Three types of glass fibers with different ―OH group concentrations have been prepared in order to compare the interactions between GF and Li-TFSI. It was found that the ―OH group concentrations on the surface of glass fiber have significant effects on interactions between glass fibers and Li-TFSI. Such interactions are crucial for both the antistatic and mechanical performances of the final PP/GF/Li-TFSI composites. The investigation indicated that the GF with high ―OH group concentrations confined the movement of TFSI-, which decreased the antistatic properties of PP/GF/Li-TFSI composites. On the other hand, the GF with low ―OH group concentrations inhibited the absorption of Li-TFSI onto the GF, which also hindered the formation of Li-TFSI conductive pathway. The best antistatic performance of the ternary composites can be achieved at the intermediate ―OH concentrations on the GF.

关键词： Polypropylene Glass fiber Li-TFSI Antistatic performance