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Genome-wide identification and expression analysis of the GhIQD gene family in upland cotton(Gossypium hirsutum L.)

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Journal of Cotton Research 2021年第1期4卷 24-37页

作者： DOU Lingling LV Limin KANG Yangyang TIAN Ruijie HUANG Deqing LI Jiayin LI Siyi LIU Fengping CAO Lingyan JIN Yuhua LIU Yang LI Huaizhu WANG Wenbo pang chaoyou SHANG Haihong ZOU Changsong SONG Guoli XIAO Guanghui School of Chemistry and Chemical Engineering Xianyang Normal UniversityXianyang 712000ShaanxiChina State Key Laboratory of Cotton Biology Institute of Cotton Research of Chinese Academy of Agricultural SciencesAnyang 455000China Zhengzhou University research base for State Key Laboratory of Cotton Biology in China ZhengzhouChina Key Laboratory of Plant Stress Biology State Key Laboratory of Cotton BiologySchool of Life SciencesHenan UniversityKaifengChina College of Life Sciences Shaanxi Normal UniversityXi’an 710119China

Background:Calmodulin(CaM)is one of the most important Ca^(2+)signaling receptors because it regulates diverse physiological and biochemical reactions in plants.CaM functions by interacting with CaM-binding proteins(CaMBPs)to modulate Ca^(2+)signaling.IQ domain(IQD)proteins are plant-specific CaMBPs that bind to CaM by their specific CaM binding sites.Results:In this study,we identified 102 GhIQD genes in the Gossypium hirsutum L.genome.The GhIQD gene family was classified into four clusters(Ⅰ,Ⅱ,Ⅲ,andⅣ),and we then mapped the GhIQD genes to the G.hirsutum L.chromosomes.Moreover,we found that 100 of the 102 GhIQD genes resulted from segmental duplication events,indicating that segmental duplication is the main force driving GhIQD gene expansion.Gene expression pattern analysis showed that a total of 89 GhIQD genes expressed in the elongation stage and second cell wall biosynthesis stage of the fiber cells,suggesting that GhIQD genes may contribute to fiber cell development in cotton.In addition,we found that 20 selected GhIQD genes were highly expressed in various tissues.Exogenous application of MeJA significantly enhanced the expression levels of GhIQD genes.Conclusions:Our study shows that GhIQD genes are involved in fiber cell development in cotton and are also widely induced by MeJA.Thw results provide bases to systematically characterize the evolution and biological functions of GhIQD genes,as well as clues to breed better cotton varieties in the future.

关键词： Gossypium hirsutum L. GhIQD genes Segmental duplication Expression analysis

Molecular Cloning and Function Analysis of Two SQUAMOSA-Like MADS-Box Genes From Gossypium hirsutum L.

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Journal of Integrative Plant Biology 2013年第7期55卷 597-607页

作者： Wenxiang Zhang Shuli Fan chaoyou pang Hengling Wei Jianhui Ma Meizhen Song Shuxun Yu State Key Laboratory of Cotton Biology Institute of Cotton Research of Chinese Academy of Agriculture Sciences College of Agronomy Northwest A & F University

The MADS-box genes encode a large family of transcription factors having diverse roles in plant development. The SQUAMOSA （SQUA）/APETALA1 （AP1）/FRUITFULL （FUL） subfamily genes are essential regulators of floral transition and floral organ identity. Here we cloned two MADS-box genes, GhMADS22 and GhMADS23, belonging to the SQUA/AP1/FUL subgroup from Gossypium hirsutum L. Phylogenetic analysis and sequence alignment showed that GhMAD$22 and GhMADS23 belonged to the euFUL and euAP1 subclades, respectively. The two genes both had eight exons and seven introns from the start codon to the stop codon according to the alignment between the obtained cDNA sequence and the Gossypium raimondii L. genome sequence. Expression profile analysis showed that GhMADS22 and GhMADS23 were highly expressed in developing shoot apices, bracts, and sepals. Gibberellic acid promoted GhMADS22 and GhMADS23 expression in the shoot apex. Transgenic Arabidopsis lines overexpressing 35S：：GhMADS22 had abnormal flowers and bolted earlier than wild type under long-day conditions （16 h light/8 h dark）. Moreover, GhMADS22 over- expression delayed floral organ senescence and abscission and it could also respond to abscisic acid. In summary, GhMADS22 may have functions in promoting flowering, improving resistance and delaying senescence for cotton and thus it may be a candidate target for promoting early-maturation in cotton breeding.

关键词： Arabidopsis transformation flowering time MADS-box SQUAMOSA-like overexpression.

Selection and Characterization of a Novel Photoperiod-Sensitive Male Sterile Line in Upland Cotton

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Journal of Integrative Plant Biology 2013年第7期55卷 608-618页

作者： Jianhui Ma Hengling Wei Ji Liu Meizhen Song chaoyou pang Long Wang Wenxiang Zhang Shuli Fan Shuxun Yu State Key Laboratory of Cotton Biology the Institute of Cotton Researchthe Chinese Academy of Agricultural Sciences College of Life Sciences Henan Normal University College of Agronomy Northwest A & F University

Upland cotton （Gossypium hirsutum L.） shows strong heterosis. However, heterosis is not widely utilized owing to the high cost of hybrid seed production. Creation of a photoperiod-sensitive genetic male sterile line could substantially reduce the cost of hybrid seed production in upland cotton. Such a mutant with virescent marker was found by space mutation in near-earth orbit and its traits had been stable after 4 years of selection in Anyang and Sanya, China. This mutant was fertile with an 11-12.5 h photoperiod when the temperature was higher than 21.5 ℃ and was sterile with a 13-14.5 h photoperiod. Genetic analysis indicated that both traits were controlled by a single recessive gene or two closely linked genes. Also, the cytological observations and transcriptome profiling analysis showed that the degradation of pollen grain cytoplasm should be the primary reason why the mutant line were male sterile under long-day conditions.

关键词： Photoperiod-sensitive genetic male sterile sterility mechanism upland cotton virescent marker.

Affects on Agronomy Traits of Different Mature Cotton Varieties（Gossypium hirsutum L.） by Late Sowing

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Affects on Agronomy Traits of Different Mature Cotton Variet...

A Genome-Scale Analysis of the PIN Gene Family Reveals Its Functions in Cotton Fiber Development

中国农学会棉花分会2017年年会暨第九次会员代表大会

作者： Song Meizhen Yu Shuxun Fan Shuli pang chaoyou Wei Hengling State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese Academy of Agricultural Sciences

Because of higher labor costs and comparative effectiveness decline in China,cotton planting areas have been declined quickly.Wheat,barley,rape and cotton double cropping system will be key cotton planting types in Yellow River and Yangtze River cotton planting areas.Shifting the growing season later may allow the crop to develop under more bloom favorable weather patterns and escape late season low temperature.The objectives were to determine the affects of late planting on agronomy traits of different mature cotton cultivars.The data of plant growth,earliness,yield and fiber quality as agronomy traits were collected from five genotypes planted at April 25（early sowing） and May 25（late sowing） in 2012 and2013 year.The results showed that plant height,branch numbers and boll numbers were significantly affected by cultivars and planting date.Date of the first square was significantly shorten and harvested rate before frost（HRBF） was significantly decreased for five different mature cotton cultivars in late sowing.The HRBF was 29.9%for Lumianyan 28,18.8%for CCRI 41 and 25.8%for CCRI 45 were significantly lower than that of 78.3%for CCRI 50 and 81.0%for CCRI 74 in late sowing.Date of the first flower,date of the first open boll of Lumianyan 28,CCRI 41 and CCRI 45 were significantly prolonged compared with that of CCRI 50 and CCRI 74 in late sowing.Number of node first sympodial branch of Lumianyan 28,CCRI 41 and CCRI 45 was significantly higher than that of CCRI 50 and CCRI 74 in late sowing.Yield and yield-related traits were significantly decreased for spring cotton varieties,Lumianyan 28,CCRI 41 and CCRI 45,but for summer cotton varieties,CCRI 50 and CCRI 74 Yield and yield-related traits were less affected by late sowing.Fiber quality was less affected for five cotton varieties.Fiber length,fiber strength and uniformity index were not significantly affected by late sowing.Fiber elongation and yellowness were significantly increased in late sowing.Therefore,in double cropping condition,the short season cotton is

关键词： cotton growth earliness yield fiber quality late sowing

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A Genome-Scale Analysis of the PIN Gene Family Reveals Its F...

中国农学会棉花分会2017年年会暨第九次会员代表大会

作者： Zhang Yuzhou He Peng Yang Zuoren Huang Gai Wang Limin pang chaoyou Xiao Hui Zhao Peng Yu Jianing Xiao Guanghui College of Life Sciences Shaanxi Normal University Institute of Cotton Research of Chinese Academy of Agricultural Sciences Peking University

The PIN-FORMED(PIN) protein,the most important polar auxin transporter,plays a critical role in the distribution of auxin and controls multiple biological processes.However,characterizations and functions of this gene family have not been identified in cotton.Here,we identified the PIN gene family in Gossypium hirsutum,G.arboreum and G.raimondii.This gene family was divided into seven subgroups.A chromosomal distribution analysis showed that GhPIN genes were evenly distributed in eight chromosomes and that the whole genome and dispersed duplications were the main duplication events for GhPIN expansion.qRT-PCR analysis showed a tissue-specific expression pattern for GhPIN.Likely due to the cis-element variations in their promoters,transcripts of PIN6 and PIN8 genes from the At(tetraploid genome orginated from G.arboreum) subgenome and PIN1 a from the Dt(tetraploid genome orginated from G.raimondii) subgenome in G.hirsutum was significantly increased compared with the transcripts in the diploids.The differential regulation of these PIN genes after the polyploidization may be conductive to fiber initiation and elongation.Exogenously applied auxin polar transport inhibitor significantly suppressed fiber growth,which is consistent with the essential function of these PIN genes in regulating cotton fiber development.Furthermore,the overexpression of GhPIN1at,GhPIN6t,and GhPIN8t in Arabidopsis promoted the density and length of trichomes in leaves.

关键词： cotton PIN-formed auxin efflux carriers fiber development expression patterns

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拟南芥核苷糖转换相关基因研究进展

生物技术通报 2009年第9期25卷 23-27页

作者：庞朝友喻树迅中国农业科学院棉花研究所安阳455112

核苷糖是植物细胞壁多聚糖合成时的活化底物。综述了近年来拟南芥核苷糖转换相关基因的克隆及功能分析等方面的研究进展。使人们能够深入认识核苷糖转换酶在植物生长发育中的重要作用,并且为人工改造植物细胞壁提供了理论依据。然而,对... 详细信息

核苷糖是植物细胞壁多聚糖合成时的活化底物。综述了近年来拟南芥核苷糖转换相关基因的克隆及功能分析等方面的研究进展。使人们能够深入认识核苷糖转换酶在植物生长发育中的重要作用,并且为人工改造植物细胞壁提供了理论依据。然而,对核苷糖转换酶的转录调控和代谢调节等方面研究还有待加强。

关键词：拟南芥核苷糖转换细胞壁

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植物MADS-box基因的研究进展

生物技术通报 2010年第8期26卷 12-19页

作者：王力娜范术丽宋美珍庞朝友喻树迅中国农业科学院棉花研究所安阳455000

MADS-box基因是一类重要的转录调控因子,在动物、植物、真菌中都有分布。在植物中从根、茎、叶到花的发育,果实的成熟MADS-box基因都起作用,尤其是在开花植物中花的发育,开花时间的控制等方面起着重要的作用。综述了MADS-box基因的分类... 详细信息

MADS-box基因是一类重要的转录调控因子,在动物、植物、真菌中都有分布。在植物中从根、茎、叶到花的发育,果实的成熟MADS-box基因都起作用,尤其是在开花植物中花的发育,开花时间的控制等方面起着重要的作用。综述了MADS-box基因的分类、进化、结构、以及MADS-box基因在植物花器官发育,开花时间的控制,果实的成熟等方面的作用。

关键词： MADS-box基因转录因子植物研究进展

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表观遗传学研究进展

生物技术通报 2011年第1期27卷 40-49页

作者：李光雷喻树迅范术丽宋美珍庞朝友中国农业科学院棉花研究所安阳455000

表观遗传学是在基因组DNA序列不发生变化的条件下,基因表达发生的改变也是可以遗传的,导致可遗传的表现型变化。表观遗传学主要包括DNA甲基化作用、组蛋白修饰作用、染色质重塑、遗传印记、随机染色体(X)失活及RNA世界等。与表观遗传学... 详细信息

表观遗传学是在基因组DNA序列不发生变化的条件下,基因表达发生的改变也是可以遗传的,导致可遗传的表现型变化。表观遗传学主要包括DNA甲基化作用、组蛋白修饰作用、染色质重塑、遗传印记、随机染色体(X)失活及RNA世界等。与表观遗传学相关的疾病主要有肿瘤、心血管病、精神病和自身免疫系统性病等。现就表观遗传学与疾病进行综述。

关键词：表观遗传学疾病 DNA甲基化组蛋白修饰癌症

棉花出苗期耐冷综合评价体系的构建及耐冷指标筛选

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中国农业科学 2022年第22期55卷 4342-4355页

作者：沈倩张思平刘瑞华刘绍东陈静葛常伟马慧娟赵新华杨国正宋美珍庞朝友中国农业科学院棉花研究所/棉花生物学国家重点实验室华中农业大学植物科学与技术学院

【目的】综合评价棉花品种（系）出苗期耐冷性，建立可靠评价模型，筛选鉴定指标，为耐冷品种选育鉴定提供简便有效的评价方法。【方法】以200份陆地棉品种（系）为试验材料，设恒定低温、昼夜变温和适宜温度3个处理，测定其出苗率、下... 详细信息

【目的】综合评价棉花品种（系）出苗期耐冷性，建立可靠评价模型，筛选鉴定指标，为耐冷品种选育鉴定提供简便有效的评价方法。【方法】以200份陆地棉品种（系）为试验材料，设恒定低温、昼夜变温和适宜温度3个处理，测定其出苗率、下胚轴长、根长、百粒重等指标，采用耐冷系数差异分析、频次分析、降幅分析、主成分分析、聚类分析和多元回归分析等方法，对群体进行耐冷型划分，并建立耐冷性评价模型和确定鉴定指标。【结果】各指标在适宜温度下变异幅度较小，变异系数为3.12%—18.89%，各品种（系）出苗率在85.00%以上，具有较高的生活力，可用于后续耐冷性分析。在低温胁迫下群体内各指标变异幅度增大，为7.14%—108.33%，在恒定低温和昼夜变温下，变幅最大的指标依次为根长和萌发指数。主成分分析将14个低温相关指标和百粒重转换为6个相互独立的综合指标，代表了全部数据74.98%的信息量。利用隶属函数法计算综合耐冷评价值（D），并对其进行聚类分析，按照耐冷性强弱将200份陆地棉品种（系）划分为5类，第Ⅰ类群属于强耐冷型共2份，第Ⅱ类群属于耐冷型共42份，第Ⅲ类群属于中耐冷型共69份，第Ⅳ类群属于较敏感型共83份，第Ⅴ类群属于敏感型共4份，其中，新陆中16号为耐冷性最强的品种。采用多元回归分析方法，建立棉花出苗期耐冷性预测模型为Y=-4.10+0.58X4+0.40X14+0.32X1+0.22X5(R2=0.92)，筛选出4个耐冷性鉴定指标，分别为恒定低温下的棉苗总长、出苗率、干物重和昼夜变温下的萌发率。田间早播试验中各品种（系）的出苗率，与室内试验结果基本一致。【结论】采用恒定低温和昼夜变温处理结合多元统计分析方法对棉花出苗期耐冷性评价是可行的，恒定低温下的棉苗总长、出苗率、干物重和昼夜变温下的萌发率，可作为鉴定指标。

关键词：棉花出苗期耐冷性综合评价指标筛选