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Identification of two distinct transactivation domains in the pluripotency sustaining factor nanog

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Cell Research 2003年第6期13卷 499-502页

作者： GUANG Jin pan, DUAN QING PEI Institute of Pharmacology, department of {2. {2. and biotechnology and State Key Labora tory of Biomembrane and Membrane biotechnology, tsinghua Institutes of Biomedicine, tsinghua university, 100084 Beijing, china. E-mail: duanqing@aol.comdepartment of Pharmacology, university of Minnesota, Minneapolis, MN55455, USA 1Institute of Pharmacology Department of Biological Sciences and Biotechnology and State Key Laboratory of Biomembrane and Membrane Biotechnology Tsinghua Institutes of Biomedicine Tsinghua University 100084 Beijing China. E-mail: duanqing@*** 2.0} of Pharmacology University of Minnesota Minneapolis MN55455 USA

Nanog is a newly identified homeodomain gene that functions to sustain the pluripotency of embryonic stem cells. However, the molecular mechanism through which nanog regulates stem cell pluripotency remains unknown. Mouse nanog encodes a polypeptide of 305 residues with a divergent homeodomain similar to those in the NK-2.family. The rest of nanog contains no apparent homology to any known proteins characterized so far. It is hypothesized that nanog encodes a transcription factor that regulates stem cell pluripotency by switching on or off target genes. To test this hypothesis, we constructed fusion proteins between nanog and DNA binding domains of the yeast transcription factor Gal4 and tested the transactivation potentials of these constructs. Our data demonstrate that both regions N- and C- terminal to the homeodomain have transcription activities. Despite the fact that it contains no apparent transactivation motifs, the C-terminal domain is about 7 times as active as the N-terminal one. This unique

关键词： nanog pluripotency stem cells self renewal transactivation domain homeoprotein homeodomain

Peroxisome proliferator-activated receptor gamma coactivator-1(PGC-1)family in physiological and pathophysiological process and diseases

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Signal Transduction and Targeted Therapy 2024年第4期9卷 1249-1292页

作者： Lu Qian Yanli Zhu Chao Deng Zhenxing Liang junmin Chen Ying Chen Xue Wang Yanqing Liu Ye Tian Yang Yang Xi'an Key Laboratory of Cardiovascular and Cerebrovascular Diseases Xi'an No.3 HospitalThe Affiliated Hospital of Northwest UniversityNorthwest UniversityXi'an 710021China Xi'an Key Laboratory of Innovative Drug Research for Heart Failure Faculty of Life Sciences and MedicineNorthwest University229 Taibai North RoadXi'an 710069China department of Cardiovascular Surgery The First Affiliated Hospital of Xi'an Jiaotong University277 Yanta West RoadXi'an 710061China department of Cardiothoracic Surgery The First Affiliated Hospital of Zhengzhou University1 Jianshe EastZhengzhou 450052China department of Hematology The First Affiliated Hospital of Xi'an JiaotongUniversity277 Yanta West RoadXi'an 710061China

Peroxisome proliferator-activated receptor gamma coactivator-1(PGC-1)family(PGC-1s),consisting of three members encompassing PGC-1a,PGC-1β,and PGC-1-related coactivator(PRC),was discovered more than a quarter-century ***-1s are essential coordinators of many vital cellular events,including mitochondrial functions,oxidative stress,endoplasmic reticulum homeostasis,and *** evidence has shown that PGC-1s are implicated in many diseases,such as cancers,cardiac diseases and cardiovascular diseases,neurological disorders,kidney diseases,motor system diseases,and metabolic *** the upstream modulators and co-activated partners of PGC-1s and identifying critical biological events modulated by downstream effectors of PGC-1s contribute to the presentation of the elaborate network of ***,discussing the correlation between PGC-1s and diseases as well as summarizing the therapy targeting PGC-1s helps make individualized and precise intervention *** this review,we summarize basic knowledge regarding the PGC-1s family as well as the molecular regulatory network,discuss the physio-pathological roles of PGc-1s in human diseases,review the application of PGC-1s,including the diagnostic and prognostic value of PGC-1s and several therapies in pre-clinical studies,and suggest several directions for future *** review presents the immense potential of targeting PGC-1s in the treatment of diseases and hopefully facilitates the promotion of PGC-1s as new therapeutic targets.

关键词： diseases life precise

Synthesis of N^(11)-anchoring biotinylated artemisinin derivatives and their preliminary biological assessment

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Science china Chemistry 2010年第1期53卷 119-124页

作者： pan QiYu1,2.WANG ShaoZhong1,LU JinJian3,MENG LingHua3 & YAO ZhuJun1,2.1Nanjing National Laboratory of Microstructures(NNLM),School of Chemistry and Chemical Engineering,Nanjing university,Nanjing 2.0093,{2. 2.oint Laboratory of Green Chemistry,{2. of Chemistry,university of Science and Technology of china,Hefei 2.002.,{2. 3State Key Laboratory of Drug Research,Shanghai Institute of Materia Medica,Shanghai Institutes for biological sciences,Chinese Academy of sciences,Shanghai 2.12.3,{2.. 1. Nanjing National Laboratory of Microstructures (NNLM) School of Chemistry and Chemical Engineering Nanjing University Nanjing 210093 China2. Joint Laboratory of Green Chemistry Department of Chemistry University of Science and Technology of China Hefei 230026 China3. State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 201203 China

Unique endoperoxide moiety of artemisinin and its derivatives has been considered the functionality exhibiting highly potent antimalarial and anticancer *** investigate the mechanisms of their biological actions,development of suitable molecular probes including biotinylated derivatives is of extreme *** synthesis and preliminary biological assessment of four new biotinylated artemisinin derivatives have been reported in this work.

关键词： artemisinin endoperoxide biotinylated derivative synthesis anticancer activity

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Enhanced Thermostability of Protein SshlOb due to Induced Conformational Entropy by the pKa Shifts of the Ionizable Residues in the Native State

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Enhanced Thermostability of Protein SshlOb due to Induced Co...

Biomarkers of neurodegenerative disorders: How good are they?

第九届全国酶学学术讨论会暨邹承鲁诞辰85周年纪念会

作者： Meng Ge Xian-Ming pan The Key Laboratory of Bioinformatics,Ministry of Education,department of {2. {2. and biotechnology,tsinghua university,Beijing,100084,china

Although the molecular underpinnings of protein thermostabilization have been the focus of many theoretical and experimental efforts,the properties leading to higher denaturation temperatures of hyperthermophilic prot... 详细信息

关键词： thermostabUity conformational entropy proline isomerization pKa shift unfolding free energy change

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Cell Research 2004年第5期14卷 347-358页

作者： Varun RACHAKONDA department of Neurology Baylor College of Medicine Houston TX USA

Biomarkers are very important indicators of normal and abnormal biological processes. Specific changes in pathologies, biochemistries and genetics can give us comprehensive information regarding the nature of any particular disease. A good biomarker should be precise and reliable, distinguishable between normal and interested disease, and differential between different diseases. It is believed that biomarkers have great potential in predicting chances for diseases, aiding in early diagnosis, and setting standards for the development of new remedies to treat diseases. New technologies have enabled scientists to identify biomarkers of several different neurodegenerative diseases. The followings, for instance, are only a few of the many new biomarkers that have been recently identified: the phosphorylated tau protein and aggregated β-amyloid peptide for Alzheimer’s disease (AD), α-synuclein contained Lewy bodies and altered dopamine transporter (DAT) imaging for Parkinson’s disease (PD), SOD mutations for familial amyotrophic lateral sclerosis (ALS), and CAG repeats resulted from Huntington’s gene mutations in Huntington’s disease (HD). This article will focus on the most-recent findings of biomarkers belonging to the four mentioned neurodegenerative diseases.

关键词： Alzheimer’s disease Parkinson’s disease Amyotrophic Lateral Sclerosis Huntington’s disease biomarkers.

Stem cell pluripotency and transcription factor Oct4

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Cell Research 2002年第Z2期 321-329页

作者： GUANG JIN pan, ZENG YI CHANG, HANS R. SCHOLER, DUANQING PEI departments of Pharmacology and {2. {2. & biotechnology, Schools of {2. and Medicine,tsinghua university, Beijing 100084, china Center for Animal Transgenesis and Germ Cell Research, School of Veterinary Medicine, department of Animal Biology, university of Pennsylvania, New Bolton Center, 382.West Street Road, Kennett Square, PA 19348, USA,department of Pharmacology, university of Minnesota, Minneapolis, MN 55455, USA

Mammalian cell totipotency is a subject that has fascinated scientists for generations. A long lasting question whether some of the somatic cells retains totipotency was answered by the cloning of Dolly at the end of the 2.th century. The dawn of the 2.st has brought forward great expectations in harnessing the power of totipotentcy in medicine. Through stem cell biology, it is possible to generate any parts of the human body by stem cell engineering. Considerable resources will be devoted to harness the untapped potentials of stem cells in the foreseeable future which may transform medicine as we know today. At the molecular level, totipotency has been linked to a singular transcription factor and its expression appears to define whether a cell should be totipotent. Named Oct4, it can activate or repress the expression of various genes. Curiously, very little is known about Oct4 beyond its ability to regulate gene expression. The mechanism by which Oct4 specifies totipotency remains entirely unresolved.

关键词： Oct4 ES cells pluripotency totipotency stem cells cloning.

Slippery hydrogel with desiccation-tolerant‘skin’for high-precision additive manufacturing

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International Journal of Extreme Manufacturing 2024年第2期6卷 486-500页

作者： Desheng Liu pan Jiang Yue Hu Yaozhong Lu Yixian Wang Jiayu Wu Danli Hu Tao Wu Xiaolong Wang State Key Laboratory of Solid Lubrication Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000People’s Republic of China CAS Key Laboratory of Chemistry of Northwestern Plant Resources Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000People’s Republic of China Shandong Laboratory of Advanced Materials and Green Manufacturing at Yantai Yantai Zhongke Research Institute of Advanced Materials and Green Chemical EngineeringYantai 264006People’s Republic of China Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region School of Chemistry and Chemical EngineeringShihezi UniversityShihezi 832003People’s Republic of China

Hydrogels inevitably undergo dehydration,structural collapse,and shrinkage deformation due to the uninterrupted evaporation in the atmosphere,thereby losing their flexibility,slipperiness,and manufacturing ***,we propose a novel bioinspired strategy to construct a spontaneously formed‘skin’on the slippery hydrogels by incorporating biological stress metabolites trehalose into the hydrogel network,which can generate robust hydrogen bonding interactions to restrain water *** contents of trehalose in hydrogel matrix can also regulate the desiccation-tolerance,mechanical properties,and lubricating performance of slippery hydrogels in a wide *** vat photopolymerization three-dimensional printing and trehalose-modified slippery hydrogels enables to achieve the structural hydrogels with high resolution,shape fidelity,and sophisticated architectures,instead of structural collapse and shrinkage deformation caused by *** thus,this proposed functional hydrogel adapts to manufacture large-scale hydrogels with sophisticated architectures in a long-term *** a proof-of-concept demonstration,a high-precision and sophisticated slippery hydrogel vascular phantom was easily fabricated to imitate guidewire ***,the proposed protocol is universally applicable to diverse types of hydrogel *** strategy opens up a versatile methodology to fabricate dry-resistant slippery hydrogel for functional structures and devices,expanding their high-precision processing and broad applications in the atmosphere.

关键词： slippery hydrogel trehalose desiccation tolerance vat photopolymerization 3D printing hydrogen bonding

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Characterization of Function of Three Domains in Dishevelled-1: DEP Domain is Responsible for Membrane Translocation of Dishevelled-1

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Cell Research 2004年第4期14卷 324-330页

作者： Wei Jun pan, Shu Zhao panG, Tao HUANG, Hui Yun GUO, Dianqing WU, Lin LI Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for {2. sciences, Chinese Academy of sciences, Graduate School of the Chinese Academy of sciences, 32. Yueyang Rd, Shanghai 2.0031 College of Life Science, Jilin university, Changchun 130061, china. department of Genetics and Developmental Biology, university of Connecticut Health Center, Farmington, Connecticut 06030, USA. 1Laboratory of Molecular Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences 320 Yueyang Rd Shanghai 200031. 2.ollege of Life Science Jilin University Changchun 130061 China. 1Laboratory of Molecular Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences 320 Yueyang Rd Shanghai 200031. 2.ollege of Life Science Jilin University Changchun 130061 China. 3department of Genetics and Developmental Biology University of Connecticut Health Center Farmington Connecticut 06030 USA. 1Laboratory of Molecular Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences 320 Yueyang Rd Shanghai 200031.

Wnt signaling plays an important role in embryogenesis and tumorgenesis. Although the mechanism about how Wnts transduce their signaling from receptor frizzled (Fz) to cytosol has not been understood, dishevelled (Dvl) protein was considered as the intersection of Wnt signal traffic. In this study, we characterized the function of three domains (DIX, PDZ and DEP) of Dvl-1 in canonical Wnt signal transduction and Dvl-1 membrane translocation. It was found both DIX and DEP domain were sufficient to block Wnt-3a-induced LEF-1 transcriptional activity and free cytosol β-catenin accumulation; whereas PDZ domain and a functional mutant form of DEP domain (DEP-KM) had no effect on canonical Wnt signaling. In addition, when cotransfected with Fz-7, DEP domain, but not DIX, PDZ or DEP-KM, translocated and co-localized with Fz-7 to the plasma membrane, which was similar to Dvl-1. Furthermore, it was DEP domain that could block Fz-7-induced membrane translocation of Dvl-1 via a possible competitive mechanism. Thes

关键词： Wnt frizzled-7 dishevelled DIX PDZ DEP membrane translocation signal transduction

Study on the catch,bycatch and discard of Chinese pelagic longline fisheries in the Atlantic Ocean

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Aquaculture and Fisheries 2024年第2期9卷 280-286页

作者： Boyi pan Jiangfeng Zhu Qinqin Lin Zhe Geng Feng Wu Yuying Zhang College of Marine sciences Shanghai Ocean UniversityShanghai201306China Key Laboratory of Sustainable Exploitation of Oceanic Fisheries Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China department of biological {2. Florida International UniversityNorth MiamiFL33181USA

Catch,bycatch and discard information is important for the assessment and management of *** Chinese pelagic tuna longline observer data from 2.10 to 2.18,we studied the catch composition in the Chinese pelagic tuna longline fisheries in Atlantic targeting bigeye tuna(Thunnus obesus)and bluefin tuna(Thunnus thynnus),and analyzed the survival status and discard rates of common bycatch species.A total of 55 species,including tunas,billfishes,sharks,sea turtles,cetaceans,seabirds,and other pelagic species,were *** results indicated that the catch composition of the Chinese pelagic tuna longline fishery targeting bigeye tuna was significantly different from that targeting bluefin *** annual discard rates of common species decreased over this *** rate by length and discard mortality for common species were varied among *** is the first study to estimate catch,bycatch,and discard using Chinese pelagic tuna longline observer data in the Atlantic Ocean,which is important for the management of Chinese tuna longline fisheries in Atlantic Ocean.

关键词： Longline fishery Catch composition Discard Size distribution Atlantic