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Unveiling the promotion role of ZnO on Zn-Al spinel oxide for CO_(2)hydrogenation

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Journal of Energy Chemistry 2025年第1期100卷 18-25页

作者： Tongyao Wang Xinlong Yao Lixin Liang Hongyu Chen pan Gao guangjin Hou State Key Laboratory of Catalysis Dalian National Laboratory for Clean EnergyDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023LiaoningChina University of Chinese Academy of Sciences Beijing 100049China

The Zn-Al spinel oxide stands out as one of the most active catalysts for high-temperature methanol synthesis from CO_(2)***,the structure–activity relationship of the reaction remains poorly understood due to challenges in atomic-level structural characterizations and analysis of reaction *** this study,we prepared two Zn-Al spinel oxide catalysts via coprecipitation(ZnAl-C)and hydrothermal(ZnAl-H)methods,and conducted a comparative investigation in the CO_(2)hydrogenation ***,under similar conditions,ZnAl-C exhibited significantly higher selectivity towards methanol and DME compared to *** characterizations using X-ray diffraction(XRD),Raman spectroscopy and electron paramagnetic resonance(EPR)unveiled that ZnAl-C catalyst had abundant ZnO species on its surface,and the interaction between the ZnO species and its ZnAl spinel oxide matrix led to the formation of oxygen vacancies,which are crucial for CO_(2)adsorption and ***,state-of-the-art solid-state nuclear magnetic resonance(NMR)techniques,including ex-situ and in-situ NMR analyses,confirmed that the surface ZnO facilitates the formation of unique highly reactive interfacial formate species,which was readily hydrogenated to methanol and *** insights elucidate the promotion effects of ZnO on the ZnAl spinel oxide in regulating active sites and reactive intermediates for CO_(2)-to-methanol hydrogenation reaction,which is further evidenced by the significant enhancement in methanol and DME selectivity observed upon loading ZnO onto the ZnAl-H *** molecular-level mechanism understandings reinforce the idea of optimizing the ZnO-ZnAl interface through tailored synthesis methods to achieve activity-selectivity balance.

关键词： CO_(2)hydrogenation Spinel oxide Zno Solid-state NMR

The N-terminal domain is a transcriptional activation domain required for Nanog to maintain ES cell self-renewal

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Chinese Science Bulletin 2009年第18期54卷 3271-3277页

作者： GUO YunQian ZHANG Juan YE Li CHEN Mo YAO Dong pan guangjin ZHANG JieQiong PEI DuanQing Laboratory of Stem Cell Biology Department of Biological Sciences & Biotechnology State Key Laboratory of Biomembrane andMembrane Biotechnology institutes of Biomedicine School of Medicine Tsinghua University Beijing 100084 China Stem Cell and Cancer Biology Group Key Laboratory of Regenerative Biology South China Institute for Stem Cell Biology and Re-generative Medicine Guangzhou Istitute of Biomedicine and Health Chinese Academy of Sciences Guangzhou 510663 China National Engineering Laboratory for Tree Breeding Key Laboratory of Genetics and Breeding in Forest Trees and OrnamentalPlants Ministry of Education Beijing Forestry University Beijing 100083 China

Nanog is a transcription factor identified by its ability to maintain the self-renewal of ES cells in the absence of leukemia inhibitory factor (LIF). Nanog protein contains an N-terminal domain (ND), a DNA-binding homeobox domain (HD) and a C-terminal domain (CD). We previously reported that the CD in Nanog is a transcriptional activation domain essential for the in vivo function of Nanog. Here we demonstrated that the ND in Nanog is also functionally important. Deletion of the ND reduces the transcriptional activity of Nanog on either artificial reporters or native Nanog promoters. This truncated Nanog is also less effective in regulating the endogenous Nanog target genes. Furthermore, the ND truncation disrupted the ability of Nanog to maintain ES cell self-renewal as well. We found that the ND is not required for the nuclear localization of Nanog. These results suggest that the regulation of endogenous pluripotent genes such as oct3/4 and rex-1 is required for the in vivo function of Nanog.

关键词： Nanog 胚胎干细胞转录激活结构域白血病抑制因子转录因子细胞重建转录活性

Variant PRC1 subunit RYBP/YAF2 forms condensate with RING1B and promotes H2AK119ub deposition

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Science China(Life Sciences) 2024年第9期67卷 2036-2038页

作者： Yanjiang Liu Gongcheng Hu Shengxiong Yang Chenghong Yan Juehan Wang guangjin pan Hongjie Yao State Key Laboratory of Respiratory Disease Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou510530China Department of Basic Research Guangzhou National LaboratoryGuangzhou 510005China CAS Key Laboratory of Regenerative Biology Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China University of Chinese Academy of Sciences Beijing 100049China

Dear Editor,Polycomb group(Pc G)proteins represent important roles in repressing gene expression throughout *** Polycomb repressive complexes(PRCs)have been subdivided into two central protein complexes,PRC1 and ***1 catalyzes H2AK119ub and PRC2catalyzes H3K27me1/2/3(Fursova et al.,2019).PRC1 is further categorized as CBX-containing canonical PRC1(c PRC1)and RYBP/YAF2-containing variant PRC1(v PRC1)(Blackledge and Klose,2021).

关键词： PRC1 RING1 RYBP

Generation of neural progenitor cells from human urine

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Generation of neural progenitor cells from human urine

Characterization of gene regulatory networks underlying key properties in human hematopoietic stem cell ontogeny

中国神经科学学会第十届全国学术会议

作者： guangjin pan Laboratory Guangzhou Institutes of Biomedicine and Health Molecular Hefei National

Neural disorders such as spinal cord injury and Parkinson’s diseases have no effective drug treatment and stem cell therapy offer perhaps the only hope for those suffering from these debilitating *** has been intense interest in obtaining human NSCs that may be used to treat these *** developed an efficient process to generate integration-free human NPCs from epithelial-like cells present in human urine *** induced NPCs derived from human urine cells（UiNPCs） could be expanded in vitro and differentiate into subtypes of mature neurons and astrocyte *** transplantation into the brain of newborn rat,the UiNPC survived well and differentiate into astrocytes and neurons in vivo,thus,suitable for potential application in regenerative medicine.

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来源： cnki会议评论

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Cell Regeneration 2024年第1期13卷 287-304页

作者： Yazhou Cuiand guangjin pan Fei Li Yanling Zhu Tianyu Wang Jun Tang Yuhua Huang Jiaming Gu Yuchan Mai Mingquan Wang Zhishuai Zhang Jiaying Ning Baoqiang Kang Junwei Wang Tiancheng Zhou Yazhou Cui guangjin pan Key Laboratory of Immune Response and Immunotherapy Joint School of Life SciencesGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou Medical UniversityGuangzhou 510530China University of Chinese Academy of Sciences Beijing 100049China Centre for Regenerative Medicine and Health Hong Kong Institute of Science and InnovationChinese Academy of SciencesHong KongChina Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine Guangdong‑Hong Kong Joint Laboratory for Stem Cell and Regenerative MedicineCenter for Cell Lineage and Cell TherapyGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China GIBH‑HKU Guangdong‑Hong Kong Stem Cell and Regenerative Medicine Research Centre GIBH‑CUHK Joint Research Laboratory On Stem Cell and Regenerative MedicineGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China South China Institute for Stem Cell Biology and Regenerative Medicine Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Key Lab for Rare&Uncommon Diseases of Shandong Province Biomedical Sciences College&Shandong Medicinal Biotechnology CentreShandong First Medical University&Shandong Academy of Medical SciencesJi’nan 250117ShandongChina

Human hematopoiesis starts at early yolk sac and undergoes site-and stage-specific changes over *** intrinsic mechanism underlying property changes in hematopoiesis ontogeny remains poorly ***,we analyzed single-cell transcriptome of human primary hematopoietic stem/progenitor cells(HSPCs)at different developmental stages,including yolk-sac(YS),AGM,fetal liver(FL),umbilical cord blood(UCB)and adult peripheral blood(PB)mobilized *** stage-specific HSPCs display differential intrinsic properties,such as metabolism,self-renewal,differentiating potentialities *** then generated highly co-related gene regulatory network(GRNs)modules underlying the differential HSC key ***,we identified GRNs and key regulators control-ling lymphoid potentiality,self-renewal as well as aerobic respiration in human *** selected regulators promotes key HSC functions in HSPCs derived from human pluripotent stem ***,GRNs underlying key intrinsic properties of human HSCs provide a valuable guide to generate fully functional HSCs in vitro.

关键词： Hematopoietic stem cell Transcription factors Lineage potential Gene regulatory networks Human induced pluripotent stem cells Hematopoietic differentiation

Neural progenitor cells from human induced pluripotent stem cells generated less autogenous immune response

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Science China(Life Sciences) 2014年第2期57卷 162-170页

作者： HUANG Ke LIU PengFei LI Xiang CHEN ShuBin WANG LiHui QIN Li SU ZhengHui HUANG WenHao LIU JuLi JIA Bei LIU Jie CAI JingLei PEI DuanQing pan guangjin Key Laboratory of Regenerative Biology Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine Guangzhou Institutes of Biomedicine and HealthChinese Academy of Sciences Department of Regeneration Medicine School of Pharmaceutical Science Jilin University Laboratory of Pathogen Biology State Key Laboratory of Respiratory Disease Department of Obstetrics and Gynecology Nanfang Hospital Southern Medical University

The breakthrough development of induced pluripotent stem cells(iPSCs)raises the prospect of patient-specific treatment for many diseases through the replacement of affected ***,whether iPSC-derived functional cell lineages generate a deleterious immune response upon auto-transplantation remains *** this study,we differentiated five human iPSC lines from skin fibroblasts and urine cells into neural progenitor cells(NPCs)and analyzed their *** co-culture with autogenous peripheral blood mononuclear cells(PBMCs),we showed that both somatic cells and iPSC-derived NPCs do not stimulate significant autogenous PBMC ***,a significant immune reaction was detected when these cells were co-cultured with allogenous ***,no significant expression of perforin or granzyme B was detected following stimulation of autogenous immune effector cells(CD3+CD8 T cells,CD3+CD8+T cells or CD3 CD56+NK cells)by NPCs in both PBMC and T cell co-culture *** results suggest that human iPSC-derived NPCs may not initiate an immune response in autogenous transplants,and thus set a base for further preclinical evaluation of human iPSCs.

关键词： induced pluripotent stem cells immunogenicity iPSC-derived neural progenitor cells

Coordination of EZH2 and SOX2 specifies human neural fate decision

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Cell Regeneration 2021年第1期10卷 316-328页

作者： Yuan Zhao Tianyu Wang Yanqi Zhang Liang Shi Cong Zhang Jingyuan Zhang Jiao Yao Qianyu Chen Xiaofen Zhong Yanxing Wei Yongli Shan guangjin pan CAS Key Laboratory of Regenerative Biology Centre for Regenerative Medicine and HealthHong Kong Institute of Science and InnovationHong KongGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China University of Chinese Academy of Sciences Beijing 100049China Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine South China Institute for Stem Cell Biology and Regenerative MedicineGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijing 100101China Biomedical Sciences College&Shandong Medicinal Biotechnology Centre Shandong First Medical University&Shandong Academy of Medical SciencesJi’nan 250062ShandongChina Nanfang Hospital Southern Medical UniversityGuangzhou 510515China Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory) Guangzhou 510005China

Polycomb repressive complexes(PRCs)are essential in mouse gastrulation and specify neural ectoderm in human embryonic stem cells(hESCs),but the underlying molecular basis remains *** in this study,by employing an array of different approaches,such as gene knock-out,RNA-seq,ChIP-seq,et al.,we uncover that EZH2,an important PRC factor,specifies the normal neural fate decision through repressing the competing meso/endoderm ***2^(−/−)hESCs show an aberrant re-activation of meso/endoderm genes during neural *** the molecular level,EZH2 represses meso/endoderm genes while SOX2 activates the neural genes to coordinately specify the normal neural ***,EZH2 also supports the proliferation of human neural progenitor cells(NPCs)through repressing the aberrant expression of meso/endoderm program during ***,our findings uncover the coordination of epigenetic regulators such as EZH2 and lineage factors like SOX2 in normal neural fate decision.

关键词： EZH2 SOX2 neural

Generation of tooth-like structures from integration-free human urine induced pluripotent stem cells

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Cell Regeneration 2013年第1期2卷 42-49页

作者： Jinglei Cai Yanmei Zhang Pengfei Liu Shubin Chen Xuan Wu Yuhua Sun Ang Li Ke Huang Rongping Luo Lihui Wang Ying Liu Ting Zhou Shicheng Wei guangjin pan Duanqing Pei CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cell Biology and Regenerative Medicine South China Institute for Stem Cell Biology and Regenerative MedicineGuangzhou Institute of Biomedicine and HealthChinese Academy of Sciences190 Kai Yuan AvenueScience ParkGuangzhou 510530P.R.China Department of Regeneration Medicine School of PharmaceuticalJilin UniversityChangchunP.R.China Department of Oral and Maxillofacial Surgery Laboratory of Interdisciplinary StudiesSchool and Hospital of StomatologyPeking UniversityBeijingP.R.China Biodynamic Optical Imaging Center(BIOPIC) Peking UniversityBeijingP.R.China The Shenzhen Key Lab of Gene and Antibody Therapy Center for Biotech&Biomedicine and Division of Life SciencesGraduate School at ShenzhenTsinghua UniversityShenzhenP.R.China Center for Biomedical Materials and Tissue Engineering Academy for Advanced Interdisciplinary StudiesPeking UniversityBeijingP.R.China.

Background:Tooth is vital not only for a good smile,but also good ***,we lose tooth regularly due to accidents or *** ideal solution to this problem is to regenerate tooth with patients’own *** we describe the generation of tooth-like structures from integration-free human urine induced pluripotent stem cells(ifhU-iPSCs).Results:We first differentiated ifhU-iPSCs to epithelial sheets,which were then recombined with E14.5 mouse dental ***-like structures were recovered from these recombinants in 3 weeks with success rate up to 30%for 8 different iPSC lines,comparable to H1 *** further detected that ifhU-iPSC derived epithelial sheets differentiated into enamel-secreting ameloblasts in the tooth-like structures,possessing physical properties such as elastic modulus and hardness found in the regular human ***:Our results demonstrate that ifhU-iPSCs can be used to regenerate patient specific dental tissues or even tooth for further drug screening or regenerative therapies.

关键词： Human urine Integration-free iPSCs Recombinant tooth Bioengineered tooth Dental epithelium

GATA2^(−/−) human ESCs undergo attenuatedendothelial to hematopoietic transition andthereafter granulocyte commitment

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Cell Regeneration 2015年第1期4卷 32-46页

作者： Ke Huang Juan Du Ning Ma Jiajun Liu Pengfei Wu Xiaoya Dong Minghui Meng Wenqian Wang Xin Chen Xi Shi Qianyu Chen Zhongzhou Yang Shubin Chen Jian Zhang Yuhang Li Wei Li Yi Zheng Jinglei Cai Peng Li Xiaofang Sun Jinyong Wang Duanqing Pei guangjin pan CAS Key Laboratory of Regenerative Biology South China Institute for Stem Cell Biology and Regenerative MedicineGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine South China Institute for Stem Cell Biology and Regenerative MedicineGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Department of Hematology Sun Yat-sen UniversityGuangzhou 510630China South China University of Technology Guangzhou 510641China Key Laboratory for Major Obstetric Diseases of Guangdong Province GuangzhouChina Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes GuangzhouChina

Background: Hematopoiesis is a progressive process collectively controlled by an elaborate network of transcriptionfactors (TFs). Among these TFs, GATA2 has been implicated to be critical for regulating multiple steps of hematopoiesisin mouse models. However, whether similar function of GATA2 is conserved in human hematopoiesis, especially duringearly embryonic development stage, is largely ***: To examine the role of GATA2 in human background, we generated homozygous GATA2 knockout humanembryonic stem cells (GATA2^(−/−) hESCs) and analyzed their blood differentiation potential. Our results demonstratedthat GATA2^(−/−) hESCs displayed attenuated generation of CD34^(+)CD43^(+) hematopoietic progenitor cells (HPCs), due tothe impairment of endothelial to hematopoietic transition (EHT). Interestingly, GATA2^(−/−) hESCs retained the potentialto generate erythroblasts and macrophages, but never granulocytes. We further identified that SPI1 downregulationwas partially responsible for the defects of GATA2^(−/−) hESCs in generation of CD34^(+)CD43^(+) HPCs and ***, we found that GATA2^(−/−) hESCs restored the granulocyte potential in the presence of Notch ***: Our findings revealed the essential roles of GATA2 in EHT and granulocyte development throughregulating SPI1, and uncovered a role of Notch signaling in granulocyte generation during hematopoiesis modeled byhuman ESCs.

关键词： hESCs GATA2 EHT HPC Granulocyte Notch signaling