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Establishment of a tetracycline-off and heat shock-on gene expression system in tobacco

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Journal of Integrative Agriculture 2017年第5期16卷 1112-1119页

作者： ZHOU You LI Jin-hua pan Yu ZHENG Yu pan yang-lu DING Yu-mei SU Cheng-gang ZHANG Xing-guo Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education/College of Horticulture and Landscape ArchitectureSouthwest UniversityChongqing 400715P.R.China

The tetracycline （Tet）-off gene expression regulation system based on the TetR-VP16/Top10 construct has not been widely utilized in plants, for its highly expressed TetR-VP16 activator is toxic to some plants and repeatedly replenishing tetracycline to turn off the constitutively active system is a tedious process. To solve these problems, a Tet-off and heat shock （HS）-on gene expression regulation system was constructed in this study. This system is composed of a chimeric transactivator gene TetR-HSF that is derived from a Tet repressor （TetR） and a HS transcription factor （HSF） controlled by a HS promoter HSP70m, and a Tet operator containing hybrid promoter, Om35S, that drives expression of the β-glucuronidase （GUS） gene. The resultant system yields a GUS expression pattern similar to that of the HSP70m promoter under inducing temperatures and at 35 and 40℃ drives GUS expression to a similar level as the Cauliflower mosaic virus （CaMV） 35S promoter. Further examination revealed that the TetR-HSF and GUS genes were induced by HS, reaching peak expression after 1 and 6 h treatment, respectively, and the HS induction of the expression system could be inhibited by Tet. This system will provide a useful tool for transgenic studies of plants in the laboratory and in the field, including transgene function analysis, agronomic trait improvement, biopharmaceutical protein production and others.

关键词： gene expression system heat shock-on tetracycline-off TetR-HSF transactivator tobacco

中国的对外直接投资是否改善了东道国的制度质量?——基于“一带一路”沿线国家的实证研究

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上海对外经贸大学学报 2017年第4期24卷 56-72页

作者：潘春阳卢德华东理工大学上海200237

"一带一路"倡议是中国在新的历史条件下开放发展的重大举措。本文致力于探索中国对外直接投资对"一带一路"沿线国家制度质量的影响。利用2003年到2014年的跨国面板数据和差分GMM方法,本文发现:第一,中国的对外直接投资强度对东道国的"... 详细信息

"一带一路"倡议是中国在新的历史条件下开放发展的重大举措。本文致力于探索中国对外直接投资对"一带一路"沿线国家制度质量的影响。利用2003年到2014年的跨国面板数据和差分GMM方法,本文发现:第一,中国的对外直接投资强度对东道国的"腐败控制"、"政府效能"、"政治稳定"、"规制质量"和"法治水平"存在显著的正效应,不但能在短期内改善东道国的制度质量,还能在长期中提高制度质量的均衡水平。第二,中国的对外直接投资对于资源较丰裕国家的制度正效应更为强劲,特别是在"政府效能"、"规制质量"和"法治水平"三个领域,从而有助于资源型国家摆脱"资源诅咒"。本文的研究对于理解中国对外直接投资的制度效应,评估"一带一路"建设的绩效和发展前景具有十分重要的现实意义。

关键词：对外直接投资 “一带一路” 制度效应差分矩估计

Defect Engineering:Can it Mitigate Strong Coulomb Effect of Mg^(2+)in Cathode Materials for Rechargeable Magnesium Batteries?

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Nano-Micro Letters 2025年第1期17卷 135-159页

作者： Zhengqing Fan Ruimin Li Xin Zhang Wanyu Zhao Zhenghui pan Xiaowei yang School of Chemistry and Chemical Engineering Shanghai Jiao Tong UniversityShanghai 200240People’s Republic of China School of Chemistry and Chemical Engineering Taiyuan University of TechnologyTaiyuan 030024People’s Republic of China School of Materials Science and Engineering Tongji UniversityShanghai 201804People’s Republic of China

Rechargeable magnesium batteries(RMBs)have been considered a promising“post lithium-ion battery”system to meet the rapidly increasing demand of the emerging electric vehicle and grid energy storage ***,the sluggish diffusion kinetics of bivalent Mg^(2+)in the host material,related to the strong Coulomb effect between Mg^(2+)and host anion lattices,hinders their further development toward practical *** engineering,regarded as an effective strategy to break through the slow migration puzzle,has been validated in various cathode materials for *** this review,we first thoroughly understand the intrinsic mechanism of Mg^(2+)diffusion in cathode materials,from which the key factors affecting ion diffusion are further ***,the positive effects of purposely introduced defects,including vacancy and doping,and the corresponding strategies for introducing various defects are *** applications of defect engineering in cathode materials for RMBs with advanced electrochemical properties are also ***,the existing challenges and future perspectives of defect engineering in cathode materials for the overall high-performance RMBs are described.

关键词： Rechargeable magnesium battery Sluggish diffusion kinetic Defect engineering Cathode materials Ion migration

Difference of Cell Wall Composition Arouses the Different Sensitivity of Brassica napus Cultivars to Boron Deficiency

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Difference of Cell Wall Composition Arouses the Different Se...

掺杂型纳米MnO_2/Al_2O_3催化剂的制备及催化臭氧化处理驱油污水二级出水

第十二届全国植物基因组学大会

作者： Yuan pan,lu yang,Fangsen Xu~* National Key Laboratory of Crop Genetic Improvement,and Microelement Research Centre,Huazhong Agricultural University, Wuhan 430070,China

Boron(B) is an essential microelement for higher *** deficiency is a widespread problem in many agricultural *** so far,B function in plant metabolism is still *** primary roles of B is the formation of cis-diol chelation with RG-Ⅱand thus orderly formation of pectin layer in cell *** understand the relationship between the composition and structure of cell wall and sensitivity difference to B deficiency in Brassica napus cultivars,we used B-efficient cultivar Qingyou 10 and B-inefficient cultivar Westar 10 to analyze their differences in cell wall pectin contents and B uptake capability.25-day-old

关键词： Brassica napus boron efficiency cell wall pectin polysaccharide

来源： cnki会议评论

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硅酸盐通报 2015年第8期34卷 2260-2266页

作者：潘璐阳王树涛张兰河尤宏东北电力大学吉林132012 哈尔滨工业大学城市水资源与水环境国家重点实验室哈尔滨150090

以Al2O3为载体,制备了掺杂型纳米Mn O2/Al2O3催化剂,考查了最佳制备条件;研究了臭氧催化氧化法处理驱油废水二级生化处理出水的效果及其影响因素。结果表明,制备的纳米Mn O2催化剂的XRD谱图中可以明显看到α-Mn O2的衍射峰;动态光散射仪... 详细信息

以Al2O3为载体,制备了掺杂型纳米Mn O2/Al2O3催化剂,考查了最佳制备条件;研究了臭氧催化氧化法处理驱油废水二级生化处理出水的效果及其影响因素。结果表明,制备的纳米Mn O2催化剂的XRD谱图中可以明显看到α-Mn O2的衍射峰;动态光散射仪（DLS）分析表明纳米Mn O2的粒度主要分布在80~180 nm之间,从XRD、扫描电镜（SEM）和透射电镜（TEM）表征可知。α-Mn O2主要呈球形,其直径在25~50 nm之间;催化剂的比表面积达到157.48 m2·g-1。催化剂的最佳焙烧温度和焙烧时间分别为550℃和4 h;活性组分含量为6%时,Mn O2/Al2O3催化剂的催化活性最强.当进水COD为100~120 mg·L-1、Mn O2/Al2O3催化剂的投量为70 mg·L-1、臭氧投量为240 mg·h-1,催化臭氧氧化30 min时,有机物的去除率达到55%以上,出水COD指标满足《污水综合排放标准》一级A标准。·OH氧化在臭氧催化氧化降解有机污染物体系中起主导作用,其平均产生速率为30.84μmmol·L-1·min-1。制备的掺杂型纳米Mn O2/Al2O3催化剂具有制备方法简单、催化效率高、使用寿命长、二次污染小等优点。

关键词：纳米MnO2 掺杂型纳米催化剂臭氧催化氧化驱油污水

前期东北大西洋-地中海西部海温异常影响河南省干热风的可能途径

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高原气象 2025年第1期44卷 150-162页

作者：竹磊磊苏晓乐潘攀吴璐杨婷中国气象局•河南省农业气象保障与应用技术重点开放实验室河南郑州450003 河南省气候中心河南郑州450003

河南省是中国冬小麦的主产区,也是较易受干热风影响的地区。目前对干热风的研究和预报多在天气尺度,从气候的角度研究干热风是否具有可预报性,具有非常重要的意义。本文基于河南省干热风资料、NCEP/NCAR再分析资料和哈得莱环流中心海温... 详细信息

河南省是中国冬小麦的主产区,也是较易受干热风影响的地区。目前对干热风的研究和预报多在天气尺度,从气候的角度研究干热风是否具有可预报性,具有非常重要的意义。本文基于河南省干热风资料、NCEP/NCAR再分析资料和哈得莱环流中心海温资料,分析了1980-2022年前期海温异常与河南省干热风日数的关系,及其可能影响途径。结果表明:(1)豫北、豫西北和豫中多年平均干热风日数较多,其他地区则相对较少。日干热风出现的概率由南向北、由5月下旬至6月上旬逐渐增加。全省平均干热风日数有显著增加趋势。(2)河南省干热风日数与当年3-4月东北大西洋-地中海西部(关键区)海温在年代际和年际尺度上均存在着密切相关,两者与干热风发生时段500 hPa高度场在去趋势前和去趋势后的显著正相关区在东亚中纬度地区位于新疆北部至贝加尔湖尔湖北部,显著负相关区位于我国东北至日本群岛附近,与850 hPa风场在去趋势前和去趋势后的相关在上述正、负显著相关区分别呈反气旋和气旋分布,气旋后部呈偏北风的显著相关,即3-4月关键区高海温年,出现的蒙古高压脊和东亚大槽造成了河南省的干热风天气。海温与环流场的相关在去趋势前和去趋势后在中纬度地区呈现出一个更明显的由东北大西洋经欧亚大陆至东北亚的纬向遥相关波列。(3)西太平洋副热带高压(以下简称副高)偏南(北)时,干热风日数易偏多(少),而3-4月关键区高(低)海温则可以导致副高偏南(北)。(4)干热风偏多年和偏少年、3-4月关键区高海温年和低海温的干热风发生时段30°N-55°N经向平均的涡度差值,及高、低海温年的干热风发生时段500 hPa准地转流函数距平和波作用通量均显示在中纬度地区存在自东北大西洋至东北亚的纬向波列。也即表明,3-4月关键区海温可以激发出一个后期干热风发生时段的类似波列,经欧亚大陆传至东北亚,而高海温年在东亚中纬度地区的环流分布造成了河南省的干热风天气。

关键词：干热风东北大西洋地中海西部海温遥相关波列

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Engineering atomic Rb-N configurations to tune radical pathways for highly selective photocatalytic H2O2 synthesis coupled with biomass valorization

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Journal of Energy Chemistry 2025年第1期100卷 215-225页

作者： Jiaye Li Shuang pan Yihuang Chen Qiong Liu College of Chemistry and Materials Engineering Wenzhou University Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology (China) Institute of Analysis Guangdong Academy of Sciences (China National Analytical CenterGuangzhou)

Photocatalytic oxygen reduction for hydrogen peroxide （H2O2） synthesis presents a green and costeffective production ***,achieving highly selective H2O2synthesis remains challenging,necessitating precise control over free radical reaction pathways and minimizing undesirable oxidative ***,we report for the visible light-driven simultaneous co-photocatalytic reduction of O2to H2O2and oxidation of biomass using the atomic rubidium-nitride modified carbon nitride（CNRb）.The optimized CNRb catalyst demonstrates a record photoreduction rate of 8.01 mM h-1for H2O2generation and photooxidation rate of 3.75 mM h-1for furfuryl alcohol to furoic acid,achieving a remarkable solar-to-chemical conversion （SCC） efficiency of up to 2.27%.Experimental characterizations and DFT calculation disclosed that the introducing atomic Rb–N configurations allows for the high-selective generation of superoxide radicals while suppressing hydroxyl free radical *** is because the Rb–N serves as the new alternative site to perceive a stronger connection position for O2adsorption and reinforce the capability to extract protons,thereby triggering a high selective redox product *** study holds great potential in precisely regulating reactive radical processes at the atomic level,thereby paving the way for efficient synthesis of H2O2coupled with biomass valorization.

关键词： Atomic economy Radical pathway regulation Rubidium atom coordination Biomass valorization

TYJL-TR2000型容错计算机联锁控制系统设计和实现

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铁道学报 2002年第4期24卷 50-54页

作者：杨扬潘明卢佩玲何梅芳铁道科学研究院通信信号研究所北京100081

在铁路车站信号计算机联锁控制系统中 ,对系统的可靠性、可用性和实时性要求较高。本单位自行设计研制了一个满足这种高可靠性、可用性和实时性要求的三模冗余容错计算机联锁控制系统 TYJL- TR2 0 0 0 。

关键词： TYJL-TR2000型容错计算机联锁控制系统铁路信号三模冗余

Staged dendrite suppression for high safe and stable lithium-sulfur batteries

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Journal of Energy Chemistry 2025年第1期100卷 674-683页

作者： Jun Jiang Zhenjie lu Yanwen Ding Shujun Liu Zhijie Qi Tian Tang Yunfan Zhang Zhiyuan Ma Jingwen Sun Liang Xue Wenyao Zhang pan Xiong Xin Wang Junwu Zhu Yongsheng Fu Key Laboratory for Soft Chemistry and Functional Materials of Ministry of Education Nanjing University of Science and Technology Jiangsu Pylon Battery Co.Ltd

The unavoidable dendrite growth and shuttle effect have long been stranglehold challenges limiting the safety and practicality of lithium-sulfur ***,we propose a dual-action strategy to address the lithium dendrite issue in stages by constructing a multifunctional surface-negatively-charged nanodiamond layer with high ductility and robust puncture resistance on polypropylene （PP） *** uniformly loaded compact negative layer can not only significantly enhance electron transmission efficiency and promote uniform lithium deposition,but also reduce the formation of dendrite during early deposition *** importantly,under the strong puncture stress encountered during the deterioration of lithium dendrite growth under limiting current,the high ductility and robust puncture resistance（145.88 MPa） of as-obtained nanodiamond layer can effectively prevent short circuits caused by unavoidable lithium *** Li||Li symmetrical cells assembled with nanodiamond layer modified PP demonstrated a stable cycle of over 1000 h at 2 mA cm-2with a polarization voltage of only 29.3 ***,the negative charged layer serves as a physical barrier blocking lithium polysulfide ions,effectively mitigating capacity *** improved cells achieved a capacity decay of only 0.042%per cycle after 700 cycles at 3 C,demonstrating effective suppression of dendrite growth and capacity attenuation,showing promising prospect.

关键词： Lithium-sulfur batteries Staged dendrite suppression Shuttle effect Surface-negatively-charged nanodiamond