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Xenoesterogens and male infertility:myth or reality?

Asian Journal of Andrology 2000年第4期2卷 263-269页

作者： Roya Rozati p.p.Reddy p.reddanna Rubina Mujtaba Assisted Conception Services Unit Mahavir Hospital and Research Centre Hyderabad(A. P.) 500 028 IndiaInstitute of Genetics and Hospital for Genetic Diseases Hyderabad IndiaDepartment of Animal Sciences School of Life Sciences University of Hyderabad IndiaAssisted Conception Services Unit Mahavir Hospital and Research Centre Hyderabad(A. P.) 500 028 India

Aim: To evaluate the role of polychlorinated biphenyls (pCBs) as a potential environmental hazard in the deteriora-tion of male fertility. Methods: Fifty-three males were studied. After a thorough case history evaluation and rele-vant clinical and laboratory investigations, pCBs were estimated in the seminal plasma of 21 infertile men with 'Unex-plained Male Factor' and 32 fertile controls. peak retention times of the eluants were compared with those of the com-mercially available standard pCB Mix, and the results confirmed spectrophotometrically. Seminal pCB concentrafonswere compared between i) fertile and infertile men and ii) men from different areas and diets. The relationship betweenpCB concentrations and measures of sperm quality such as the total motile sperm count, was assessed. Results:pCBs were detected in seminal plasma of infertile men but absent from controls. Sperm quantity and quality were sig-nificantly lower in infertile men compared to controls. The highest average pCB concentrations were found in fish-eat-ing urban dwellers, and followed in succession by fish-eating rural dwellers, non fish-eating urban dwellers and nonfish-eating mral dwellers. The total motile sperm counts were inversely proportional to the pCB concentrations and weresignificantly lower than those of the respective controls. Conclusion: pCBs may be instrumental in the deteriorationof sperm quantity and quality, a contaminated fish diet being the main source of exposure.(Asian J Androl 2000 Dec; 2: 263-269 )

关键词： polychlorinated biphenyls xenoestrogens male infertility sperm

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purification and characterization of rat testicular glutathione S-transferases:role in the synthesis of eicosanoids

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Asian Journal of Andrology 2000年第4期2卷 277-282页

作者： D.Anuradha K.VeeraReddy T.CharlesKumar S.Neeraja p.R.K.Reddy p.reddanna School of Life Sciences University of Hyderabad Hyderabad-500 046 IndiaSchool of Life Sciences University of Hyderabad Hyderabad-500 046 IndiaSchool of Life Sciences University of Hyderabad Hyderabad-500 046 IndiaSchool of Life Sciences University of Hyderabad Hyderabad-500 046 IndiaSchool of Life Sciences University of Hyderabad Hyderabad-500 046 IndiaSchool of Life Sciences University of Hyderabad Hyderabad-500 046 India

Aim: purification of glutathione S-transferases (GSTs) from rat testis; separation and identificationunits and their role in eicosanoid biosynthesis. Methods: purification of mt testicular GSTs by affit?phy, employing S-hexylglutathione-linked epoxy-activated Sepharose 6B column and separation of indiby reverse phase-high pressure liquid chromatography (Rp-HpLC). Characterization of affinity purified,um dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-pAGE) and Westem blot analysis. The roGSTs in eicosanoid biosynthesis was determined by incubating GSTs with 5, 6-Leukotriene A_4Me (prostaglandin H_2 (pGH_2) and analyzing the products formed on HpLC/TLC. Results: The present stumajority of rat testicular GSTs are of Y_b size (60%) with molecular weight of 27 kDa. The most preunits, however, are GST Y_(n2) (27% ), followed by GST Y_c (24% ) and GST Y_(nl) (20%). These testiculavery high Leukotriene C_4 (LTC_4) synthase activity with 5, 6-Leukotriene A4Me (LTA_4Me) as theprostaglandin D (pGD) synthase activity with prostaglandin H_2 (pGH_2) as the substrate. Conclusion:testicular GSTs are Y_b sized and are involved in the synthesis of eicosanoids like LTC_4 and pGD_2.(Asian J Androl 2000 Dec; 2: 277-282)

关键词： testis glutathione transferase leukotrienes prostaglandins

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Novel functional association of rat testicular membrane-associated cytosolic glutathione S transferases and cyclooxygenase in vitro

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Asian Journal of Andrology 2005年第2期7卷 171-178,页

作者： S.Neeraja B.Ramakrishna A.S.Sreenath G.V.Reddy p.R.K.Reddy p.reddanna Department of Animal Sciences School of Life SciencesUniversity of HyderabadHyderabad 500046India

Aim:To analyze the role of cytosolic glutathione S-transferases (cGSTs) and membrane-associated cytosolic GSTs (macGSTs) in prostaglandin biosynthesis and to evaluate the possible interaction between glutathione S-transferases (GSTs) and cyclooxygenase (COX) in ***:SDS-pAGE analysis was undertaken for characterization of GSTs,thin layer chromatography (TLC) to monitor the effect of GSTs on prostaglandin biosynthesis from arachi- donic acid (AA) and spectrophotometric assays were done for measuring activity levels of COX and ***: SDS-pAGE analysis indicates that macGSTs have molecular weights in the range of 25-28 *** a coupled assay involving GSTs,arachidonic acid and cyclooxygenase-1,rat testicular macGSTs produced prostaglandin E2 and F2~, while the cGSTs caused the generation of prostaglandin D2,E2 and F_(2α).In vitro interaction studies on GSTs and COX at the protein level have shown dose-dependent inhibition of COX activity by macGSTs and vice *** effect, however,is not seen with *** inhibitory effect of COX on macGST activity was relieved with increasing concentrations of reduced glutathione (GSH) but not with 1-chloro 2,4-dinitrobenzene (CDNB).The inhibition of COX by macGSTs,on the other hand,was potentiated by ***:We isolated and purified macGSTs and cGSTs from rat testis and analyzed their involvement in prostaglandin *** studies reveal a revers- ible functional interaction between macGSTs and COX in vitro,with possible interactions between them at the GSH binding site of macGSTs.

关键词： glutathione S-transferase cyclooxygenase arachidonic acid glutathione prostaglandins

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