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purification and characterization of rat testicular glutathione S-transferases:role in the synthesis of eicosanoids

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Asian Journal of Andrology 2000年第4期2卷 277-282页

作者： D.Anuradha k.Veerareddy T.Charleskumar S.Neeraja p.r.k.reddy p.reddanna School of Life Sciences University of Hyderabad Hyderabad-500 046 IndiaSchool of Life Sciences University of Hyderabad Hyderabad-500 046 IndiaSchool of Life Sciences University of Hyderabad Hyderabad-500 046 IndiaSchool of Life Sciences University of Hyderabad Hyderabad-500 046 IndiaSchool of Life Sciences University of Hyderabad Hyderabad-500 046 IndiaSchool of Life Sciences University of Hyderabad Hyderabad-500 046 India

Aim: purification of glutathione S-transferases (GSTs) from rat testis; separation and identificationunits and their role in eicosanoid biosynthesis. Methods: purification of mt testicular GSTs by affit?phy, employing S-hexylglutathione-linked epoxy-activated Sepharose 6B column and separation of indiby reverse phase-high pressure liquid chromatography (rp-HpLC). Characterization of affinity purified,um dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-pAGE) and Westem blot analysis. The roGSTs in eicosanoid biosynthesis was determined by incubating GSTs with 5, 6-Leukotriene A_4Me (prostaglandin H_2 (pGH_2) and analyzing the products formed on HpLC/TLC. results: The present stumajority of rat testicular GSTs are of Y_b size (60%) with molecular weight of 27 kDa. The most preunits, however, are GST Y_(n2) (27% ), followed by GST Y_c (24% ) and GST Y_(nl) (20%). These testiculavery high Leukotriene C_4 (LTC_4) synthase activity with 5, 6-Leukotriene A4Me (LTA_4Me) as theprostaglandin D (pGD) synthase activity with prostaglandin H_2 (pGH_2) as the substrate. Conclusion:testicular GSTs are Y_b sized and are involved in the synthesis of eicosanoids like LTC_4 and pGD_2.(Asian J Androl 2000 Dec; 2: 277-282)

关键词： testis glutathione transferase leukotrienes prostaglandins

Novel functional association of rat testicular membrane-associated cytosolic glutathione S transferases and cyclooxygenase in vitro

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Asian Journal of Andrology 2005年第2期7卷 171-178,页

作者： S.Neeraja B.ramakrishna A.S.Sreenath G.V.reddy p.r.k.reddy p.reddanna Department of Animal Sciences School of Life SciencesUniversity of HyderabadHyderabad 500046India

Aim:To analyze the role of cytosolic glutathione S-transferases (cGSTs) and membrane-associated cytosolic GSTs (macGSTs) in prostaglandin biosynthesis and to evaluate the possible interaction between glutathione S-transferases (GSTs) and cyclooxygenase (COX) in ***:SDS-pAGE analysis was undertaken for characterization of GSTs,thin layer chromatography (TLC) to monitor the effect of GSTs on prostaglandin biosynthesis from arachi- donic acid (AA) and spectrophotometric assays were done for measuring activity levels of COX and ***: SDS-pAGE analysis indicates that macGSTs have molecular weights in the range of 25-28 *** a coupled assay involving GSTs,arachidonic acid and cyclooxygenase-1,rat testicular macGSTs produced prostaglandin E2 and F2~, while the cGSTs caused the generation of prostaglandin D2,E2 and F_(2α).In vitro interaction studies on GSTs and COX at the protein level have shown dose-dependent inhibition of COX activity by macGSTs and vice *** effect, however,is not seen with *** inhibitory effect of COX on macGST activity was relieved with increasing concentrations of reduced glutathione (GSH) but not with 1-chloro 2,4-dinitrobenzene (CDNB).The inhibition of COX by macGSTs,on the other hand,was potentiated by ***:We isolated and purified macGSTs and cGSTs from rat testis and analyzed their involvement in prostaglandin *** studies reveal a revers- ible functional interaction between macGSTs and COX in vitro,with possible interactions between them at the GSH binding site of macGSTs.

关键词： glutathione S-transferase cyclooxygenase arachidonic acid glutathione prostaglandins

Small molecule inhibitor DDQ-treated hippocampal neuronal cells show improved neurite outgrowth and synaptic branching

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Neural regeneration research 2025年第9期20卷 2624-2632页

作者： Jangampalli Adi pradeepkiran priyanka rawat Arubala p.reddy Erika Orlov pHemachandra reddy Department of Internal Medicine Texas Tech University Health Sciences CenterLubbockTXUSA Department of Nutritional Sciences College of Human SciencesTexas Tech UniversityLubbockTXUSA Department of pharmacology&Neuroscience Texas Tech University Health Sciences CenterLubbockTXUSA Department of Neurology Texas Tech University Health Sciences CenterLubbockTXUSA Department of Speech Language and Hearing SciencesTexas Tech University Health Sciences CenterLubbockTXUSA Department of public Health Texas Tech University Health Sciences CenterLubbockTXUSA

The process of neurite outgrowth and branching is a crucial aspect of neuronal development and *** and dendrites,sometimes referred to as neurites,are extensions of a neuron's cellular body that are used to start *** we explored the effects of diethyl(3,4-dihydroxyphenethylamino)(quinolin-4-yl)methylphosphonate(DDQ)on neurite developmental features in HT22 neuronal *** this work,we examined the protective effects of DDQ on neuronal processes and synaptic outgrowth in differentiated HT22cells expressing mutant Tau(mTau)*** investigate DDQ chara cteristics,cell viability,biochemical,molecular,western blotting,and immunocytochemistry were *** outgrowth is evaluated through the segmentation and measurement of neural *** neural processes can be seen and measured with a fluorescence microscope by manually tracing and measuring the length of the neurite *** neuronal processes can be observed and quantified with a fluorescent microscope by manually tracing and measuring the length of the neuronal ***-treated mTau-HT22 cells(HT22 cells transfected with cDNA mutant Tau)were seen to display increased levels of synaptophysin,MAp-2,andβ-***,we confirmed and noted reduced levels of both total and p-Tau,as well as elevated levels of microtubule-associated protein 2,β-tubulin,synaptophysin,vesicular acetylcholine transporter,and the mitochondrial biogenesis protein-pe roxisome prolife rator-activated receptor-gamma coactivator-1α.In mTa u-expressed HT22 neurons,we observed DDQ enhanced the neurite characteristics and improved neurite development through increased synaptic *** findings conclude that mTa u-HT22(Alzheimer's disease)cells treated with DDQ have functional neurite developmental chara *** key finding is that,in mTa u-HT22 cells,DDQ preserves neuronal structure and may even enhance nerve development function with mTa u inhibition.

关键词： diethyl(3 4-dihydroxyphenethylamino)(quinolin-4-yl)methylphosphonate(DDQ) hippocampal neuronal cells HT22 neurite outgrowth neuronal development small molecule

Lora Sense:Sensing and Optimization of Lora Link Behavior Using path-Loss Models in Open-Cast Mines

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Computer Modeling in Engineering & Sciences 2025年第1期142卷 425-466页

作者： Bhanu pratap reddy Bhavanam prashanth ragam School of Computer Science and Engineering VIT-AP UniversityAmaravati52237India

The Internet of Things(IoT)has orchestrated various domains in numerous applications,contributing significantly to the growth of the smart world,even in regions with low literacy rates,boosting socio-economic *** study provides valuable insights into optimizing wireless communication,paving the way for a more connected and productive future in the mining *** IoT revolution is advancing across industries,but harsh geometric environments,including open-pit mines,pose unique challenges for reliable *** advent of IoT in the mining industry has significantly improved communication for critical operations through the use of radio Frequency(rF)protocols such as Bluetooth,Wi-Fi,GSM/GprS,Narrow Band(NB)-IoT,SigFox,ZigBee,and Long range Wireless Area Network(LoraWAN).This study addresses the optimization of network implementations by comparing two leading free-spreading IoT-based rF protocols such as ZigBee and *** field tests are conducted in various opencast mines to investigate coverage potential and signal *** is tested in the Tadicherla open-cast coal mine in ***,LoraWAN field tests are conducted at one of the associated cement companies(ACC)in the limestone mine in Bargarh,India,covering both Indoor-toOutdoor(I2O)and Outdoor-to-Outdoor(O2O)environments.A robust framework of path-loss models,referred to as Free space,Egli,Okumura-Hata,Cost231-Hata and Ericsson models,combined with key performance metrics,is employed to evaluate the patterns of signal *** field testing and careful data analysis revealed that the Egli model is the most consistent path-loss model for the ZigBee protocol in an I2O environment,with a coefficient of determination(r^(2))of 0.907,balanced error metrics such as Normalized root Mean Square Error(NrMSE)of 0.030,Mean Square Error(MSE)of 4.950,Mean Absolute percentage Error(MApE)of 0.249 and Scatter Index(SI)of *** the O2O scenario,the Ericsson model

关键词： Internet of things long range wireless area network ZigBee mining environments path-loss models coefficient of determination mean square error

Multilocular thymic cysts-a diagnostic challenge on computed tomography

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World Journal of Clinical Cases 2025年第3期13卷 61-65页

作者： Nirmal kumar reddy Onteddu Naga Sai rasagna Mareddy Sai Swarupa r Vulasala Jayabharath Onteddu Mayur Virarkar Department of Internal Medicine University of Florida College of MedicineJacksonvilleFL 32209United States Department of Diagnostic radiology University of AlabamaBirminghamAL 35294United States Department of Diagnostic radiology University of Florida College of MedicineJacksonvilleFL 32209United States Department of public Health Sciences University of FloridaGainesvilleFL 32611United States

A recent case report provided a patient scenario,wherein,a 39-year-old male patient presented with occasional palpitations,headache,and *** of tumor markers did not show any abnormal ***,a computed tomography(CT)scan was undertaken,and its findings were affirmative of thymic ***,the postoperative histopathological assessment of the mass,after its resection,confirmed it as an anterior mediastinal multilocular thymic cyst(MTC),with concurrent acute upper respiratory tract infection and acute ***,this case report advocates the need for a preoperative histopathological examination with CT imaging to minimize the risk of confusing an MTC with a malignant thymic tumor.

关键词： Imaging findings Multilocular thymic cyst Histopathology Thymic cancer Mediastinal mass

A comparative analysis of unsteady and steady Buongiorno’s Williamson nanoliquid flow over a wedge with slip effects

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Chinese Journal of Chemical Engineering 2020年第7期28卷 1767-1777页

作者： r.Chandra Sekhar reddy p.Sudarsana reddy Department of Mathematics Rajeev Gandhi Memorial Collge of Engineering&TechnologyNandyalA.P.India

Comparison between unsteady and steady MHD Buongiorno’s model Williamson nanoliquid flow through a wedge with slip effects,chemical reaction and radiation is made in this *** and Brownian motion are also considered in this *** similarity variables are presented to transmute the governing pDEs into the set of non-linear *** most widely authenticated finite element method is implemented to analyze these set of ODEs *** behavior of concentration,temperature and velocity sketches for varied values of relevant parameters is numerically calculated and the outcomes are plotted through *** numerical values of dimensionless rates of mass transfer,heat and velocity are also evaluated and depicted through *** is noted that with upsurging values of angle of wedge parameter,the distributions of temperature of the liquid intensify in both steady and unsteady cases.

关键词： Finite element method Chemical reaction Williamson nanoliquid Thermophoresis Thermal radiation Brownian motion

Thermal radiation effect on unsteady threedimensional MHD flow of micropolar fluid over a horizontal surface of a parabola of revolution

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propulsion and power research 2022年第1期11卷 129-142页

作者： S.r.r.reddy p.Bala Anki reddy Center for Nonlinear Systems Chennai Institute of TechnologyChennai600069T.N.India Department of Mathematics S.A.S.Vellore Institute of TechnologyVellore632014T.N.India

This paper explores the time-dependent magnetohydrodynamics(MHD)micropolar fluid flow over a three-dimensional variable stretching surface in the occurrence of radiation *** model time-dependent partial differential equations(pDE’s)in three independent variables are transformed into ordinary differential equations(ODE’s)by the suitable selfsimilarity *** perturbation method(HpM)and runge-kutta(rk)4^(th) order method along with shooting technique are used in the present *** also,HpM results are compared with runge-kutta(rk)4^(th) order method along with the shooting *** velocity,micro rotation in x and y directions,temperature,skin friction factor and heat transfer rates are examined for the emerging *** velocity profiles and momentum boundary layer thickness intensification with increasing values of the vortex viscosity *** higher value of a magnetic parameter declines the skin friction *** type of investigation may be profitable to the polymer fluids,exotic lubricants,electronic chips,artificial fibers,drawing of copper wires,etc.

关键词： radiation parameter Magnetohydrodynamics Micropolar fluid Variable thickness sheet Homotopy perturbation method(HpM)

Spectral change and far-red emission of Mn^(2+)ions co-doped NaSrB_(5)O_(9):Dy^(3+)luminescence material for plant growth LEDs

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Journal of rare Earths 2022年第2期40卷 218-225,I0002页

作者： k.Munirathnam koduru Mallikarjuna r.Vijaya p.C.Nagajyothi k.ramakrishna reddy M.Srinivas Department of physics School of Applied SciencesREVA UniversityBengaluru560064India Department for Management of Science and Technology Development Ton Due Thang UniversityHo Chi Minh CityVietnam Faculty of Applied Sciences Ton Due Thang UniversityHo Chi Minh CityVietnam Department of physics Visvodaya Government Degree CollegeVenkatagiri524132India School of Mechanical Engineering Yeungnam UniversityGyeongsan33541South Korea Department of Chemistry School of Applied SciencesREVA UniversityBengaluru560064India

Due to the characteristic emission of phosphors,phosphor-converted LEDs have been employed to provide the requisite light sources for indoor plant growth in the optical agricultural ***,we prepared a series of Mn^(2+)co-doped NaSrB5 O9:Dy^(3+)phosphors via a solid-state reaction *** phosphors have significant three-band emissions at 467 nm(blue region),606 nm(orange region),and765 nm(far-red region),ascribed to the^(4)F_(9/2)-^(6)H_(15/2)and 4 F9/2-6 H13/2 transitions of the Dy^(3+)ions and the^(4)T_(1g)(G)-^(6)A_(1g)(S)spin-forbidden transition of the Mn^(2+)ions,respectively,when excited by light of 376 nm(near-UV region).The co-dopant in the host material facilitates tunable photoluminescence(pL)due to energy transfer from the Dy^(3+)ions to the Mn^(2+)*** three emission peaks from the prepared phosphors well match with the absorption spectra of the photosynthesis pigments of plants,chlorophyll and phytochrome,which can absorb blue(400-500 nm),orange-red(550-700 nm),and infrared(Ir)radiation,indicating that these phosphors have potential applications in the fabrication of plant-growth *** to the pL studies,the structure of the phosphors was determined by X-ray diffraction,refined by rietveld method and chemically quantified by X-ray photoelectron spectroscopy.

关键词： phosphors refinement X-ray photoelectron spectroscopy Energy transfer plant growth LEDs rare earths

Investigation of strong shock wave interactions with CeO2 ceramic

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Journal of Advanced Ceramics 2014年第4期3卷 297-305页

作者： Vishakantaiah JAYArAM Asha GUpTA k.p.J.reddy Shock Induced Materials Chemistry Lab SSCUIndian Institute of ScienceBangalore-560012India Texas Materials Institute the University of Texas at AustinAustinTexas 78712USA Department of Aerospace Engineering Indian Institute of ScienceBangalore-560012India

Strong shock wave interactions with ceramic material ceria(CeO_(2))in presence of O_(2)and N2 gases were investigated using free piston driven shock tube(FpST).FpST is used to heat the test gas to very high temperature of about 6800-7700 k(estimated)at pressure of about 6.8-7.2 Mpa for short duration(2-4 ms)behind the reflected shock *** is subjected to super heating and cooling at the rate of about 106 k/*** of CeO_(2)sample was done before and after exposure to shock heated test gases(O_(2)and N2).The surface composition,crystal structure,electronic structure and surface morphology of CeO_(2)ceramic were examined using X-ray photoelectron spectroscopy(XpS),X-ray diffraction(XrD),Fourier transform infrared(FTIr)spectrometry,scanning electron microscopy(SEM)and high resolution transmission electron microscopy(HrTEM).results obtained from the experimental investigations show that CeO_(2)can withstand high pressure accompanied by thermal shock without changing its crystal *** CeO_(2)releases lattice oxygen making it possible to shift between reduced and oxidized states upon the interaction with shock heated *** to such reaction mechanism,CeO_(2)ceramic undergoes nitrogen doping with decrease in lattice *** reveal that CeO_(2)retains its crystal structure during strong shock interaction,even at elevated pressure.

关键词： shock tube shock interaction high enthalpy gas ceria nitrogen-doped surface reaction