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Insulin-like growth factor-1 mRNA isoforms and insulinlike growth factor-1 receptor mRNA expression in chronic hepatitis C

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World Journal of Gastroenterology 2015年第13期21卷 3867-3875页

作者： Aldona Kasprzak Agnieszka Adamek Wieslawa przybyszewska przemyslaw pyda Jacek Szmeja Agnieszka Seraszek-Jaros Agata Lanzafame Anna Surdacka Iwona mozer-Lisewska Maria Koczorowska Department of Histology and Embryology Poznan University of Medical Sciences60-781 PoznanPoland Department of Infectious Diseases Poznan University of Medical Sciences61-285 PoznanPoland Department of General Surgery Gastroenterological Oncology and Plastic SurgeryPoznan University of Medical Sciences60-355 PoznanPoland Department of Bioinformatics and Computational Biology Chair of Clinical PathomorphologyPoznan University of Medical Sciences60-529 PoznanPoland Department and Clinics of Conservative Dentistry and periodontology Poznan University of Medical Sciences60-812PoznanPoland Department of Molecular Virology Adam Mickiewicz University61-614 PoznanPoland

AIM: to evaluate the expression of different insulinlike growth factor(IGF)-1 mRNA isoforms and IGF-1 receptor(IGF-1R) mRNA in hepatitis C virus(HCV)-infected livers. METHODS: Thirty-four liver biopsy specimens from chronic hepatitis C(CH-C) patients were obtained before anti-viral therapy. Inflammatory activity(grading) and advancement of fibrosis(staging) were evaluated using a modified point scale of METAVIR. The samples were analyzed using quantitative real-time pCR technique. From fragments of liver biopsies and control liver that were divided and ground in liquid nitrogen, RNA was isolated using RNeasy Fibrous Tissue Mini Kit according to the manufacturer's instruction. Expression levels of IGF-1 mRNA isoforms(IGF-1A, IGF-1B, IGF-1C, p1, and p2) and IGF-1R mRNA were determined through normalization of copy numbers in samples as related to reference genes: glyceraldehyde-3-phosphate dehydrogenase and hydroxymethylbilane synthase. Results on liver expression of the IGF-1 mRNA isoforms and IGF-1R transcript were compared to histological alterations in liver biopsies and with selected clinical data in the patients. Statistical analysis was performed using Statistica pL v. 9 software. RESULTS: The study showed differences in quantitative expression of IGF-1 mRNA variants in HCV-infected livers, as compared to the control. Higher relative expression of total IGF-1 mRNA and of IGF-1 mRNAs isoforms(p1, A, and C) in HCV-infected livers as compared to the control were detected. Within both groups, expression of the IGF-1A mRNA isoform significantly prevailed over expressions of B and C isoforms. Expression of p1 mRNA was higher than that of p2 only in CH-C. Very high positive correlations were detected between reciprocal expressions of IGF-1 mRNA isoforms p1 and p2(r = 0.876). Expression of p1 and p2 mRNA correlated with IGF-1A mRNA(r = 0.891; r = 0.821, respectively), with IGF-1B mRNA(r = 0.854; r = 0.813, respectively), and with IGF-1C mRNA(r = 0.839; r = 0.741, respe

关键词： Chronic hepatitis C Insulin-like growth factor-1 receptor Insulin-like growth factor-1 mRNA isoforms Quantitative polymerase chain reaction

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