检索结果-南通市图书馆

Study of intracellular anabolism of 5-fluorouracil and incorporation in nucleic acids based on an LC-HRMS method

journal of pharmaceutical Analysis 2021年第1期11卷 77-87页

作者： Christelle Machon Frederic Catez Nicole Dalla Venezia Floriane Vanhalle Laetitia Guyot Anne Vincent Maxime Garcia Beatrice Roy jean-jacques Diaz jerome Guitton Laboratoire de Chimie Analytique Faculte de Pharmacie de LyonUniversite Lyon 18 Avenue RockefellerLyon69373France Laboratoire de Biochimie et de pharmaco-toxicologie Centre Hospitalier Lyon-SudeHCLPierre Benite69495France Inserm U1052 CNRS UMR5286 Centre de Recherche en Cancerologie de LyonF-69000 LyonFrance.Centre Leon BerardF-69008 LyonFranceUniversite de Lyon 1F-69000 LyonFrance Institut des Biomolecules Max Mousseron(IBMM) UMR 5247 CNRSUniversite de MontpellierENSCMCampus TrioletMontpellierFrance Laboratoire de toxicologie Faculte de pharmacie de Lyon8 avenue RockefellerF-69373 LyonFrance

5-Fluorouracil(5-FU)is an anticancer drug extensively used for different *** metabolic activation leads to several nucleoside and nucleotide metabolites essential to exert its cytotoxic activity on multiple cellular targets such as enzymes,DNA and *** this paper,we describe the development of a method based on liquid chromatography coupled with high resolution mass spectrometry suitable for the simultaneous determination of the ten anabolic metabolites(nucleoside,nucleotide and sugar nucleotide)of *** chromatographic separationwas optimized on a porous graphitic carbon column allowing the analysis of the metabolites of 5-FU as well as endogenous *** detection was performed on an Orbitrap■tandem mass *** of the method was verified in intracellular content and in RNA *** limit of detection was equal to 12 pg injected on column for nucleoside metabolites of 5-FU and 150 pg injected on column for mono-and tri-phosphate nucleotide *** effect was evaluated in cellular contents,DNA and RNA extracts for nucleoside and nucleotides *** method was successfully applied to i)measure the proportion of each anabolic metabolite of 5-FU in cellular contents,ii)follow the consequence of inhibition of enzymes on the endogenous nucleotide pools,iii)study the incorporation of metabolites of 5-FU into RNA and DNA,and iv)to determine the incorporation rate of 5-FUrd into 18 S and 28 S sub-units of rRNA.

关键词： 5-Fluorouracil LC-MS-HRMS Nucleotide RNA DNA Incorporation rate

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Ribavirin:past,present and future

引用

World journal of Hepatology 2016年第2期8卷 123-130页

作者： Véronique Loustaud-Ratti Marilyne Debette-Gratien jérémie jacques Sophie Alain pierre Marquet DenisSautereau Annick Rousseau paul Carrier Fédération Hépatologie CHU Dupuytren87042 LimogesFrance Service d’Hépatogastroentérologie CHU Limoges87042 LimogesFrance U850 INSERM Universitéde LimogesCHU Limoges87042 LimogesFrance Service de Bactériologie Virologie CHU Limoges87042 LimogesFrance U1092 INSERM Universitéde LimogesCHU Limoges87042 LimogesFrance Service de pharmacologie CHU Limoges87042 LimogesFrance

Before the advent of direct acting antiviral agents(DAAs) ribavirin, associated to pegylated-interferon played a crucial role in the treatment of chronic hepatitis C, preventing relapses and breakthroughs. In the present era of new potent DAAs, a place is still devoted to the drug. Ribavirin associated with sofosbuvir alone is efficient in the treatment of most cases of G2 infected patients. All options currently available for the last difficult-to-treat cirrhotic G3 patients contain ribavirin. Reducing treatment duration to 12 wk in G1 or G4 cirrhotic compensated patients is feasible thanks to ribavirin. Retreating patients with acquired anti NS5 A resistance-associated variants using ribavirin-based strategies could be useful. The addition of ribavirin with DAAs combinations however, leads to more frequent but mild adverse events especially in cirrhotic patients. preliminary data with interferon-free second generation DAAs combinations without ribavirin suggest that future of the drug is jeopardized even in difficult-totreat patients: The optimization of ribavirin dosage according to an early monitoring of blood levels has been suggested to be relevant in double therapy with peginterferon or sofosbuvir but not with very potent combinations of more than two DAAs.

关键词： Ribavirin Hepatitis C peginterferon Directacting antiviral agents

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Kinematic History and Changes in the Tectonic Stress Regime during the Cenozoic along the Qinling and Southern Tanlu Fault Zones

引用

Acta Geologica Sinica(English Edition) 1999年第3期73卷 264-274页

作者： ZHANG Yueqiao pierre Vergèly jacques-Louis Mercier WANG Yongmin ZHANG Yong HUANG Dezhi Institute of Geomechanics Chinese Academy of GeologyBeijing 100081China URA-CNRS:Géophysique et Géodynamique Interne Universitéde Parie-SudBatment 509F-91405Orsay-CedexFrance Institute of Geology Bureau of Geology and Mineral Resouces of Anhui ProvinceHefeiChina

Since the mid-late Eocene, North China has been subjected to extensional stress, resulting in the formation and development of basins. The dynamic origin of this crustal extension has long been an issue of debate. This paper presents the results of kinematic analyses of faults obtained from two seperated areas in North China. In the Weihe graben situated on the southernmost margin of the Ordos block, analyses of fault kinematics were coupled with an analysis of the basin's subsidence history. Three successive extensional tectonic phases accompaning the basin's formation and development have been distinguished. The palaeogene extension was oriented in a WNW-ESE direction; the Neogene extension in a NE-SW direction and the pliocene-Quaternary extension in a NW-SE direction. Such changes have also been recorded by fault kinematics along the southern Tanlu fault zone. This has been demonstrated by three successive sets of fault striations indicating normal dip slip resulting from NW-SE extension, then left-lateral slip with a normal component resulting from NE-SW extension, and right-lateral slip with a minor normal component, respectively. The kinematic history of faults and their chronological evolution indicate changes in continental dynamics acting in North China over Cenozoic time.

关键词： fault kinematics tectonic stress regime Qinling and Tanlu fault zones

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Glycerol—A Viable Solvent for Absorption of Highly polar Solutes I: Behaviour of Molecular Interactions

Glycerol—A Viable Solvent for Absorption of Highly Polar So...

引用

作者： jacques j.Scheepers Edison Muzenda Department of Chemical Engineering Faculty of Engineering and the Built EnvironmentUniversity of Johannesburg

This work focused on the potential of glycerol as a scrubbing solvent for stripping of highly polar volatile organic compounds(VOCs) from industrial waste gas streams. Results for glycerol interactions were compared to those of water. Solubility predictions in the form of infinite dilution activity coefficients were made using the Modified UNIFAC Dortmund group contribution model, which was set up on a Microsoft Excel spreadsheet specifically designed for this purpose. Activity coefficients for VOCs in water and glycerol(highly polar solvents) increased with an increase in the length of the VOC solute nonpolar tail. Furthermore, activity coefficients decreased with an increase in the degree of branching of the VOC solute. The results show that low chain length highly polar alcohol, ketone, organic acid and aldehyde solutes are very soluble in glycerol. Diols, dicarbonyl aldehydes, some ketone derivatives and the ethanolamines also show good solubility. Highly polar diesters and ester derivatives are less soluble in glycerol and water. This study has found glycerol to be a better scrubbing solvent than water in treating highly polar VOCs. However it is possible that the standard method of building up the glycerol molecule in the UNIFAC may result in under-predictions of activity coefficients and thus special group interaction parameters for glycerol are required.

关键词： Activity coefficients glycerol solubility UNIFAC van der Waals

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Effect of preoperative renin-angiotensin system blockade on vasoplegia after cardiac surgery:A systematic review with metaanalysis

引用

World journal of Cardiology 2022年第4期14卷 250-259页

作者： jean jacques Noubiap Brice Nouthe Ying Tung Sia Marco Spaziano Centre for Heart Rhythm Disorders The University of AdelaideAdelaide 5000South AustraliaAustralia Department of Medicine University of British ColumbiaVancouver V6T 1W5Canada Department of Medicine Regional Trois-Rivières Hospital(CIUSSS-MCQ)Trois-Rivières 5000Canada Department of Cardiology McGill University Health CentreMontréal QC H4A 3J1Canada

BACKGROUND Vasoplegia is a common complication of cardiac surgery but its causal relationship with preoperative use of renin angiotensin system(RAS)blockers[angiotensin converting enzyme inhibitors(ACEIs)and angiotensin receptor blockers(ARB)]is still *** To update and summarize data on the effect of preoperative use of RAS blockers on incident *** All published studies from MEDLINE,EMBASE,and Web of Science providing relevant data through january 13,2021 were identified.A random-effects metaanalysis method was used to pool estimates,and post-cardiac surgery shock was differentiated from *** Ten studies reporting on a pooled population of 15672 patients(none looking at ARBs exclusively)were included in the *** were case-control *** of ACEIs was associated with an increased risk of vasoplegia[pooled adjusted odds ratio(Aor)of 2.06,95%CI:1.45-2.93]and increased inotropic/vasopressor support requirement(pooled aOR 1.19,95%CI:1.10-1.29).post-cardiac surgery shock was increased in the presence of left ventricular dysfunction(pooled aOR 2.32,95%CI:1.60-3.36;I249%)but not increased by the use of beta blockers(pooled aOR 0.78,95%CI:0.36-1.69;I277%).Two randomized control trials(RCTs),not eligible for the meta-analysis,did not show an association between continuation of RAS blockers and *** preoperative continuation of ACEIs is associated with an increased need for inotropic support postoperatively and with an increased risk of vasoplegia in observational studies but not in *** absence of a consensus definition of vasoplegia should lead to the use of perioperative cardiovascular monitoring when designing RCTs to better understand this discrepancy.

关键词： Vasoplegia Cardiac surgery Coronary artery bypass graft Angiotensin converting enzyme inhibitors

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Obscure bleeding colonic duplication responds to proton pump inhibitor therapy

引用

World journal of Gastroenterology 2013年第35期19卷 5940-5942页

作者： jérémie jacques Fabrice projetti Romain Legros Virginie Valgueblasse Matthieu Sarabi paul Carrier Fabien Fredon Stéphane Bouvier Véronique Loustaud-Ratti Denis Sautereau Service d’Hépato-gastro-entérologie CHU Dupuytren

We report the case of a 17-year-old male admitted to our academic hospital with massive rectal *** childhood he had reported recurrent gastrointestinal bleeding and had two exploratory laparotomies 5and 2 years *** emergency abdominal computed tomography scan,gastroscopy and colonoscopy,performed after hemodynamic stabilization,were considered ***-dose intravenous proton pump inhibitor(ppI)therapy was initiated and bleeding stopped *** other massive rectal bleeds occurred 8 h after each cessation of ppI which led to a hemostatic laparotomy after negative gastroscopy and small bowel capsule *** showed long tubular duplication of the right colon,with fresh blood in the duplicated *** lower gastrointestinal bleeding is a difficult medical situation and potentially *** presence of ulcerated ectopic gastric mucosa in the colonic duplication explains the partial efficacy of ppI *** gastrointestinalbleeding responding to empiric anti-acid therapy should probably evoke the diagnosis of bleeding ectopic gastric mucosa such as Meckel’s diverticulum or gastrointestinal duplication,and gastroenterologists should be aware of this potential medical situation.

关键词： Colonic duplication Gastro-intestinal duplication Gastrointestinal bleeding Hemostatic colorectal surgery proton pump inhibitor therapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

蒿甲醚对感染小鼠体内埃及血吸虫皮层的损害(英文)

引用

中国寄生虫学与寄生虫病杂志 2006年第6期24卷 425-432页

作者：肖树华 Marcel TANNER 沈炳贵 jürg UTZINGER jacques CHOLLET 中国疾病预防控制中心寄生虫病预防控制所世界卫生组织血吸虫病疟疾和丝虫病合作中心上海200025 Swiss Tropical Institute P.O. Box CH-4002 Basel Switzerland

目的评价蒿甲醚对小鼠体内埃及血吸虫皮层的损害作用。方法8只小鼠于感染埃及血吸虫尾蚴后81d,用单剂蒿甲醚400mg/kg口服治疗。治疗后1、3、7和14d各剖杀2只小鼠,用灌注法收集血吸虫,并按常规方法固定和处置虫体,作扫描电镜观察。从另2... 详细信息

目的评价蒿甲醚对小鼠体内埃及血吸虫皮层的损害作用。方法8只小鼠于感染埃及血吸虫尾蚴后81d,用单剂蒿甲醚400mg/kg口服治疗。治疗后1、3、7和14d各剖杀2只小鼠,用灌注法收集血吸虫,并按常规方法固定和处置虫体,作扫描电镜观察。从另2只未作治疗的感染小鼠取虫作对照。结果用蒿甲醚治疗后24h,雄虫的皮层结节肿大、破溃或从皮层上剥落;在雄虫和雌虫的体表可查见有局灶性或广泛的皮层肿胀、融合、空泡变化、糜烂和剥落,以及感觉结构的破坏。治疗后3d,雌、雄虫的皮层损害加重,最严重的损害为口吸盘肿胀和破溃,并查见皮层褶嵴有广泛和严重的肿胀、糜烂和剥落,以及雌虫盘状感觉结构的破坏。治疗后7至14d,有些虫仍示有中或重度皮层损害,而有些仍存活的虫则示其大部分皮层已有明显恢复。结论蒿甲醚对埃及血吸虫的皮层具有广泛和严重的损害作用。

关键词：蒿甲醚血吸虫病埃及血吸虫扫描电镜术

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

蒿甲醚对感染小鼠体内埃及血吸虫超微结构的影响

引用

中国寄生虫学与寄生虫病杂志 2006年第5期24卷 321-328页

作者：肖树华 jürg UTZINGER 沈炳贵 Marcel TANNER jacques CHOLLET 中国疾病预防控制中心寄生虫病预防控制所上海200025 Swiss Tropical Institutep.O.BoxCH-4002 BaselSwitzerland

目的观察蒿甲醚对小鼠体内埃及血吸虫成虫超微结构的损害。方法8只小鼠于感染埃及血吸虫尾蚴后81d用单剂蒿甲醚400mg/kg口服治疗。治后24h、3d、7d和14d各剖杀2只小鼠,用灌注法收集血吸虫,并按常规方法固定和处置虫体,作透射电镜观察。... 详细信息

目的观察蒿甲醚对小鼠体内埃及血吸虫成虫超微结构的损害。方法8只小鼠于感染埃及血吸虫尾蚴后81d用单剂蒿甲醚400mg/kg口服治疗。治后24h、3d、7d和14d各剖杀2只小鼠,用灌注法收集血吸虫,并按常规方法固定和处置虫体,作透射电镜观察。从另2只未治疗的感染小鼠体内取虫作对照。结果蒿甲醚对血吸虫皮层超微结构的损害主要是皮层基质的肿胀、溶解和空泡变化,基底膜消失和部份受损皮层破裂;在感觉器和皮层结节中,常见其内部结构广泛溶解。在肌层、实质组织、合体细胞和肠管上皮细胞中,查见局灶性或广泛的溶解、粗面内质网减少及线粒体空泡变化和变性。雌虫卵黄细胞的严重变化是空泡变化、粗面内质网减少、卵黄球融合以及受损卵黄细胞破溃等。上述雌、雄虫变化于感染小鼠用蒿甲醚治疗后24h即可见到,并逐渐加重,3～7d后最重。治后14d,部分雌、雄虫仍示有超微结构的损害,但同时亦观察到受损虫组织的恢复。结论蒿甲醚对埃及血吸虫成虫的皮层和皮层下组织具有广泛和严重的超微结构损害。

关键词：蒿甲醚血吸虫病埃及血吸虫亚显微结构透射电镜术

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Anti-hepatitis C virus drugs and kidney

引用

World journal of Hepatology 2016年第32期8卷 1343-1353页

作者： paul Carrier Marie Essig Marilyne Debette-Gratien Denis Sautereau Annick Rousseau pierre Marquet jérémie jacques Véronique Loustaud-Ratti U850 INSERM Université de Limoges Service d’Hépato-gastroentérologie CHU Limoges

Hepatitis C virus(HCV) mainly targets the liver but can also induce extrahepatic manifestations. The kidney may be impacted via an immune mediated mechanism or a cytopathic effect. HCV patients are clearly at a greater risk of chronic kidney disease(CKD) than uninfected patients are, and the presence of CKD increases mortality. Interferon-based therapies and ribavirin are difficult to manage and are poorly effective in endstage renal disease and hemodialysis. These patients should be given priority treatment with new direct anti-viral agents(DAAs) while avoiding peginterferon and ribavirin. The first results were convincing. To aid in the correct use of these drugs in patients with renal insufficiency, their pharmacokinetic properties and potential renal toxicity must be known. The renal toxicity of these new drugs was not a safety signal in clinical trials, and the drugs are generally efficient in these frail populations. These drugs are usually well tolerated, but recent cohort studies have demonstrated that these new regimens may be associated with renal side effects, especially when using sofosbuvir combinations. HCV, renal diseases and comorbidities are intimately linked. The close monitoring of renal function is required, particularly for at-risk patients(transplanted, HIV-coinfected, CKD, hypertensive or diabetic patients). New DAA regimens, which will soon be approved, will probably change the landscape.

关键词： Nephrotoxicity Hepatitis C Direct anti-viral agents Kidney End-stage renal disease

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Mimotope peptide modified pompon mum-like magnetic microparticles for precise recognition,capture and biotransformation analysis of rituximab in biological fluids

引用

Acta pharmaceutica Sinica B 2024年第3期14卷 1317-1328页

作者： jiawen Yang Aixuan Zhou Minyi Li Qiaoxian He jingwei Zhou jacques Crommen Wentao Wang Zhengjin jiang Qiqin Wang Institute of pharmaceutical Analysis College of Pharmacy/State Key Laboratory of Bioactive Molecules and Druggability Assessment/International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education(MOE)of ChinaJinan UniversityGuangzhou 510632China Laboratory for the Analysis of Medicines Department of Pharmaceutical SciencesCIRMUniversity of LiegeLiege B-4000Belgium SCIEX Shanghai 200050China

Due to low immobilized ligand density,limited binding capacity,and severe interference from serum proteins,developing ideal peptide-based biomaterials for precise recognition and in vivo analysis of biopharmaceuticals remains a huge *** this study,mimotope peptide modified pompon mum-like biomimetic magnetic microparticles(MMps,3.8μm)that mimic the specific functionalities of CD20 on malignant B cells were developed for the first *** from the numerous ligand binding sites(Ni^(2+))on the pompon mum-like MMps,these novel materials achieved≥10 times higher peptide ligand densities(>2300 mg/g)and antibody binding capacities(1380 mg/g)compared to previous reported *** the high specificity of the mimotope peptide,rituximab can be precisely recognized and enriched from cell culture media or serum *** also established an LC-MS/MS method using the MMps for tracking rituximab biotransformation in patient ***,deamidation of Asn55 and Asn33,as well as oxidation of Met81 and Met34 were observed at the key complementarity determining regions of rituximab,which could potentially influence antibody function and require careful ***,these versatile biomimetic MMps demonstrate superior recognition and enrichment capabilities for target antibodies,offering interesting possibilities for biotransformation analysis of biopharmaceuticals in patient serum.

关键词： Therapeutic monoclonal antibody Mimotope peptide precise recognition peptide-based biomaterials Biotransformation patient serum

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：