Introduction Ectopic pregnancy(Ep)poses significant health risks,particularly in developing nations,necessitating improved diagnostic *** study aimed to explore potential biomarkers for Ep *** A case-control study was...
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Introduction Ectopic pregnancy(Ep)poses significant health risks,particularly in developing nations,necessitating improved diagnostic *** study aimed to explore potential biomarkers for Ep *** A case-control study was conducted at the Sri Ramachandra Institute of Higher Education and Research,Chennai,Tamil *** included 140 Ep cases and 140 pregnant controls,aged 19-38 years,attending routine *** samples were analysed for beta-human chorionic gonadotropin,progesterone,soluble tms-like tyrosine kinase-1(sFLT-1)and eight microRNAs(miRs).Results Ditferential expression of biomarkers was observed in Ep *** miRs(hsa-miR-141,hsa-miR-218,hsa-miR-519d and hsa-miR-873)were downregulated,and four miRs(***-miR-517a,hsa-miR-523 and hsa-miR-323-3p)were *** significant expression fold changes were noted(ppt for hsa-miR-141 and ***-519d exhibited promising diagnostic potential with the highest specificity(97.1%)and a sensitivity of 47.1%.sFLT-1,as an individual marker,demonstrated a sensitivity of 98.6% and a specificity of 90%.The combination of sFLT-1 and miR-519d significantly enhanced the sensitivity to 100% with a specificity of 87.1%.Conclusions The combination of miR-519d and sFLT-1 emerges as a promising diagnostic tool for Ep,offering a sensitivity of 100% and a specificity of 87.1%.These findings underscore the potential of biomarker-based approaches in improving Ep diagnosis,especially in resource-limited *** validation and clinical implementation studies are warranted to corroborate these findings and enhance Ep management strategies.
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