检索结果-南通市图书馆

Effect of irrigation water containing arsenic on elemental composition of bean and lettuce plants cultivated in calcareous sandy soil

引用

食物生产加工与营养(英文) 2019年第1期1卷 1-10页

作者： Sirat Sandil péter dobosy Krisztina Kröpfl Anna Füzy MihályÓvári Gyula Záray MTA Centre for Ecological Research Danube Research InstituteKarolinaút 29-31Budapest H-1113Hungary Cooperative Research Centre of Environmental Sciences Eötvös Loránd UniversityPázmány Péter sétány 1/ABudapest H-1117Hungary MTA Centre for Agricultural Research Institute for Soil Sciences and Agricultural ChemistrHerman Ottóút 15Budapest H-1022Hungary

Background:The uptake of arsenic by vegetables from soil irrigated with arsenic enriched groundwater poses a major health *** edible portion of these vegetables transfer arsenic to the human *** uptake of arsenic was studied in bean(phaseolus vulgaris L.)and lettuce(Lactuca sativa L.)in a controlled greenhouse pot culture with calcareous sandy soil as *** plants were irrigated with water containing sodium arsenate at concentrations 0.1,0.25 and 0.5 mg L^(-1).The total arsenic concentration of the different plants parts was determined by ICp-MS,following microwave-assisted acid *** change in plant biomass production and essential macroelements(Mg,p,K)and microelements concentration(Fe,Mn,Cu,Zn)was also ***:The As concentration in the bean was in the order:root>stem>leaf>bean fruit and in lettuce:root>*** the highest dose(0.5 mg L^(-1))the As concentration in the bean fruit and lettuce leaves was 22.1μg kg^(-1)and 1207.5μg kg^(-1)DW,*** As concentration in the irrigation water resulted in decreased edible biomass production in bean,while in lettuce the edible biomass production *** plant exhibited any visible toxicity *** significant change was observed in the macro and microelements *** total and the water-soluble arsenic in soil amounted to 3.5 mg kg^(−1)and 0.023 mg kg^(−1),*** transfer factor was found to increase with increase in the As treatment *** transfer factor range for bean from root to fruit was 0.003–0.005,and for lettuce from root to leaves was 0.14–***:Considering the FAO-WHO recommended maximum tolerable daily intake(MTDI)limit of 2.1μg kg^(-1)body weight,and the biomass production,both plants should not be cultivated at As treatment level higher than 0.1 mg L^(-1).

关键词： Arsenic irrigation water uptake transfer factor calcareous sandy soil vegetables

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A High-performing and Cost-Effective SNp Genotyping Method Using rhpCR and Universal Reporters

引用

Advances in Bioscience and Biotechnology 2018年第9期9卷 497-512页

作者： Kristin Beltz Daniel Tsang Junzhou Wang Scott Rose Yun Bao Yu Wang Katelyn Larkin Susan Rupp Daniela Schrepfer Krishnalekha Datta Keith Gunderson Chris Sailor Scott Hansen Joseph dobosy Lynette Lewis Aurita Menezes Joseph Walder Mark Behlke Caifu Chen Genetic Analysis R&D Integrated DNA Technologies Coralville IA USA

We have developed a novel dual enzyme chemistry called rhAmp®SNp genotyping based on RNase H2-dependent pCR (rhpCR) that provides high signal and specificity for SNp analysis. rhAmp SNp genotyping combines a unique two-enzyme system with 3’ end blocked DNA-RNA hybrid primers to interrogate SNp loci. Activation of the blocked primers occurs upon hybridization to its perfectly matched target, which eliminates or greatly reduces primer dimers. A thermostable hot-start RNase H2 cleaves the primer immediately 5’ of the ribose sugar, releasing the blocking group and allowing primer extension. pCR specificity is further improved with the use of a mutant Taq DNA polymerase, resulting in improved allelic discrimination. Signal generation is obtained using a universal reporter system which requires only two reporter probes for any bi-allelic SNp. 1000 randomly selected SNps were chosen to validate the 95% design rate of the design pipeline. A subsampling of 130 human SNp targets was tested and achieved a 98% call rate, and 99% call accuracy. rhAmp SNp genotyping assays are compatible with various qpCR instruments including QuantStudioTM 7 Flex, CFX384TM, IntelliQube®, and Biomark HDTM. In comparison to TaqMan®, rhAmp SNp genotyping assays show higher signal (Rn) and greater cluster separation, resulting in more reliable SNp genotyping performance. The rhAmp SNp genotyping solution is suited for high-throughput SNp genotyping applications in humans and plants.

关键词： SNp Genotyping RNase H2 rhpCR rhAmp SNp Genotyping Universal Reporter

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：