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Bio-Design and Manufacturing 2024年第5期7卷 624-636页

作者： Bilgesu Kaya ozlem yesil-Celiktas Department of Bioengineering Faculty of EngineeringEge UniversityIzmir 35100Türkiye Translational Pulmonary Research Center(EgeSAM) Ege UniversityIzmir 35100Türkiye

The plausibility of human exposure to particulate matter(PM)has witnessed an increase within the last several *** of different sizes has been discovered in the atmosphere given the role of dust transport in weather and climate *** a regulator,the lung epithelium orchestrates the innate response to local ***,we developed a lung epithelium-ona-chip platform consisting of easily moldable polydimethylsiloxane layers along with a thin,flexible,and transparent ionic liquid-based poly(hydroxyethyl)methacrylate gel *** epithelium was formed through the culture of human lung epithelial cells(Calu-3)on this *** mechanical stress at the air–liquid interface during inhalation/exhalation was recapitulated using an Arduino-based servo motor system,which applied a uniaxial tensile strength from the two sides of the chip with 10%strain and a frequency of 0.2 ***,the administration of silica nanoparticles(PM0.5)with an average size of 463 nm to the on-chip platform under static,dynamic,and dynamic+mechanical stress(DMS)conditions demonstrated the effect of environmental pollutants on lung *** viability and release of lactate dehydrogenase were determined along with proinflammatory response through the quantification of tumor necrosis factor-α,which indicated alterations in the epithelium.

关键词： Ionic liquid-based membrane Lung Epithelial barrier Mechanostress Organ-on-chip Silica particles

A 3D in vitro co-culture model for evaluating biomaterial-mediated modulation of foreign-body responses

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Bio-Design and Manufacturing 2022年第3期5卷 465-480页

作者： Betul Cakmak Pelin Saglam-Metiner Goze Beceren Yu S.Zhang ozlem yesil-Celiktas Department of Bioengineering Faculty of EngineeringEge University35100 IzmirTurkey Division of Engineering in Medicine Brigham and Women’s HospitalDepartment of MedicineHarvard Medical SchoolCambridgeMAUSA

The immune response after implantation of a biomaterial may shorten the functional life of the implant,depending on the degree of the *** this study,we used a polyacrylamide-alginate(PAAm-Alg)hydrogel,which has been previously characterized as a biocompatible material and shown to enhance regeneration of cartilage in vivo,along with a graphiteenhanced hydrogel(PAAm-Alg-G)as a non-biocompatible counterpart,to evaluate macrophage attachment and polarization to pro-or anti-inflammatory *** performance of each biomaterial in the presence of fibroblasts and chondrocytes was validated by an in vitro model which demonstrated modulation of the foreign-body response.A blend of 5%gelatin methacryloyl and 0.1%methacrylated hyaluronic acid was optimized to mimic the extracellular matrix(ECM)and support cell viability,proliferation,migration,and functionality at an initial concentration of 3.25×10^(5) cells/*** PAAm-Alg-G hydrogel localized in the simulated ECM showed cytotoxic and genotoxic effects for both fibroblasts and chondrocytes,while exhibiting a proliferative effect on macrophages with elevated immune *** M1/M2 ratio was 0.73 for PAAm-Alg hydrogel but 2.64 for PAAm-Alg-G,leading to significant M1 dominance(p0.05).The interleukin-6(IL-6)concentration secreted in the presence of PAAm-Alg hydrogel(4.58 pg/mL)significantly decreased(p<0.0001)on day 13,while the increase(p<0.0001)in interleukin-10(IL-10)concentration(120.73 pg/mL)confirmed the switch from a pro-inflammatory to an anti-inflammatory *** immune responses by developing a simplistic yet powerful three-dimensional in vitro model provides advantages in preparing for clinical use of biomaterials.

关键词： Biomaterial Macrophage polarization Genotoxicity Biocompatibility Immune response

A hepatocellular carcinoma–bone metastasis-on-a-chip model for studying thymoquinone-loaded anticancer nanoparticles

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Bio-Design and Manufacturing 2020年第3期3卷 189-202页

作者： Fatemeh Sharifi ozlem yesil-Celiktas Aslihan Kazan Sushila Maharjan Saghi Saghazadeh Keikhosrow Firoozbakhsh Bahar Firoozabadi Yu Shrike Zhang Division of Engineering in Medicine Department of MedicineBrigham and Women’s HospitalHarvard Medical SchoolCambridgeMAUSA School of Mechanical Engineering Sharif University of TechnologyTehranIran Department of Bioengineering Faculty of EngineeringEge UniversityBernovaIzmirTurkey

We report the development of a metastasis-on-a-chip platform to model and track hepatocellular carcinoma(HCC)-bone metastasis and to analyze the inhibitory effect of an herb-based compound,thymoquinone(TQ),in hindering the migration of liver cancer cells into the bone *** bioreactor consisted of two chambers,one accommodating encapsulated HepG2 cells and one bone-mimetic niche containing hydroxyapatite(HAp).Above these chambers,amicroporous membrane was placed to resemble the vascular barrier,where medium was circulated over the *** was observed that the liver cancer cells proliferated inside the tumor microtissue and disseminated from the HCC chamber to the circulatory flow and eventually entered the bone *** number of metastatic HepG2 cells to the bone compartment was remarkably higher in the presence of HAp in the *** was then used as ametastasis-controlling agent in both free form and encapsulated nanoparticles,to analyze its suppressing effect on HCC *** indicated that the nanoparticle-encapsulated TQ provided a longer period of inhibitory *** summary,HCC-bone metastasis-on-a-chip platform was demonstrated to model certain key aspects of the cancer metastasis process,hence corroborating the potential of enabling investigations on metastasis-associated biology as well as improved anti-metastatic drug screening.

关键词： Tumor-on-a-chip Organ-on-a-chip Hydroxyapatite Hepatocellular carcinoma Thymoquinone

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橡子单宁的提取优化及其在皮革中的应用

中国皮革 2014年第9期43卷 44-44,45-49页

作者： Ersin Onem Gurbuz Gulumser Seref Akay ozlem yesil Celiktas 土耳其爱琴海大学工程学院皮革工程系土耳其博尔诺瓦35100 土耳其爱琴海大学工程学院生物工程系土耳其博尔诺瓦35100

优化了从橡子中提取单宁的最佳工艺参数，并考察其鞣制性能。研究了溶剂／原料比（20～100mL／g）、时间（2～10h）和混合溶剂比（甲醇一水）（0～100％）对提取率、单宁含量和单宁总量的影响。将最佳优化条件下提取出的橡杭单宁应用于... 详细信息

优化了从橡子中提取单宁的最佳工艺参数，并考察其鞣制性能。研究了溶剂／原料比（20～100mL／g）、时间（2～10h）和混合溶剂比（甲醇一水）（0～100％）对提取率、单宁含量和单宁总量的影响。将最佳优化条件下提取出的橡杭单宁应用于皮革鞣制中，并测定鞣革的填充系数（％）和收缩温度（Ts）。优化的提取参数如下：溶剂／原料比为100mL／g，提取时间为6h，混合溶剂比为62％甲醇-38％水。采用提取出的单宁鞣后成革的填充系数为57．81％，收缩温度为75．5℃，远好于市售橡杭单宁鞣革（52．83％，73℃），凸显出优化过程的重要性。表明使用提取出的橡杭单宁作为植物鞣剂应用于皮革工业中，鞣制效果令人满意。

关键词：皮革橡子橡椀单宁优化响应曲面法鞣制

<i>Mycobacterium vaccae</i>Immunization to Pregnant BALB/c Mice Ameliorated Lung Histopathology and Bone Marrow Eosinophila in Ovalbumin Sensitized Offsprings

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Open Journal of Immunology 2014年第2期4卷 31-41页

作者： Tunc Akkoc Cevdet Ozdemir Didem Yazi ozlem yesil Nerin Nadir Bahceciler Isil Barlan Pediatric Allergy and Immunology Division School of Medicine Marmara University Istanbul Turkey

Context: One of the treatment strategies for atopic diseases is to skew immune response away from Th2 dominance by using Mycobacterial strains. Objective: We wanted to find out whether M. vaccae administration to pregnant mice had any preventive effect on the offsprings in the development of a murine asthma model. Materials and Methods: Pregnant BALB/c mice were divided into two groups;first group received heat-killed M. vaccae subcutaneously on 12th day of pregnancy and the latter group received PBS. After delivery, newborn mice of each group were further divided into two subgroups as M. vaccae/Ovalbumin (OVA), M. vaccae/control, PBS/OVA and PBS/ control. To establish experimental murine asthma model, mice were intraperitoneally sensitized and challenged intratracheally with Ovalbumin. We analysed airway histopathology, bone marrow eosinophil progenitors and splenic cell cytokine profiles of the offsprings. Results: Comparison of offsprings in M. vaccae/OVA group were not different than PBS controls with respect to thicknesses of airway epithelium, basement membrane, subepithelial smooth muscles and number of hyperplasic goblet cells as well as bronchial associated lymphoid tissue density and eosinophil progenitors in the bone marrow. Comparison of M. vaccae/OVA group to asthma model revealed significant differences and lower levels of OVA-induced IL-5. Conclusions: We propose that immunization of pregnant BALB/c with M. vaccae could prevent histopathological alterations in the airways related to the asthma model and down-regulates IL-5 secretion from splenocytes of offsprings.

关键词： BALB/c Murine Asthma Model Mycobacterium vaccae Ovalbumin Pregnancy

Evaluation the Vickers Hardness of Denture Base Materials

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Open Journal of Stomatology 2016年第4期6卷 114-119页

作者： Zeynep yesil Duymus Alper Ozdogan Hamza Ulu ozlem Ozbayram Department of Prosthodontics Faculty of Dentistry Recep Tayyip Erdo?an University Rize Turkey Department of Prosthodontics Faculty of Dentistry Atatürk University Erzurum Turkey

The aim of this study was to evaluate the Vickers Hardness of four different denture base materials. Four different denture base materials were used in this study: Heat-cured pink acrylic resin, self-cured acrylic resin, heat-cured clear acrylic resin and polyamide denture base materials. To-tally 80 disc shaped samples which 2 mm thickness and 40 mm diameter were prepared. The samples were stored in distilled water after polished. Then, Vickers Hardness test was performed with TMTeck HV-1000B (200 gram at 15 second). The statistically analysis of obtained data was performed with use one-way analysis of variance test. The results showed that the highest Vickers Hardness value (18.57 HV) was identified in heat-cured (pink) acrylic resin, the lowest Vickers Hardness value (9.62 HV) was identified in polyamide denture base materials. The result of one-way analysis of variance test showed that there were statistically very highly significant differences between four denture base materials of Vickers Hardness (p < 0.001). The obtained data presented that the self-cured denture base acrylic resin was the hardnest denture base materials.

关键词： Hardness Denture Bases Polyamide