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Antimicrobial resistance and underlying mechanisms in Staphylococcus aureus isolates

Asian Pacific Journal of Tropical Medicine 2017年第11期10卷 1059-1064页

作者： Ebru Sebnem Yilmaz ozkan aslantas Department of Biology Faculty of Art and Sciences Mustafa Kemal University Department of Microbiology Faculty of Veterinary Medicine Mustafa Kemal University

Objective: To investigate the antimicrobial susceptibility of 97 clinical Staphylococcus aureus(S. aureus) strains against 14 antimicrobials and corresponding resistance ***: The antimicrobial susceptibility of the isolates was determined using a disk diffusion method and antimicrobial resistance genes were screened by polymerase chain reaction. Mutations responsible for ciprofloxacin and rifampicin resistance were investigated by polymerase chain reaction and DNA ***: All isolates were found to be susceptible to vancomycin. Various rates of resistance to penicillin(83.5%), ampicillin(77.3%), erythromycin(63.9%), tetracycline(16.5%), amoxicillin/clavulanic acid(16.5%), ciprofloxacin(15.5%), trimethoprim/sulfamethoxazole(15.5%), oxacillin(13.4%), fusidic acid(12.4%), rifampin(6.2%), clindamycin(6.2%), gentamicin(6.2%) and mupirocin(5.2%) were determined. In addition,different combinations of resistance genes were identified among resistant *** resistant isolates had mutations in codon 84(Ser84 Leu) and 106(Gly106 Asp) in the gyr A gene. Mutations in grl A were mostly related to Ser80 Phe substitution. Leu466 Ser mutation in the rpo B gene was detected in all rifampin resistant isolates. All methicillin resistant S. aureus isolates were SCCmec type ***: In conclusion, it was determined that the isolates were resistant to different classes of antimicrobials at varying rates and resistance was mediated by different genetic mechanisms. Therefore, continuous monitoring of resistance in S. aureus strains is necessary to control their resistance for clinically important antimicrobials.

关键词： Staphylococcus aureus Antimicrobial resistance Resistance mechanisms Point mutation

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Effect of cryogenic and aging treatments on low-energy impact behaviour of Ti-6Al-4V alloy

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Transactions of Nonferrous Metals Society of China 2017年第3期27卷 514-526页

作者： Y. PEKBEY K. aslantas Y. PEKBEY Department of Mechanical Engineering University of Afyon Kocatepe Afyon-03000 Turkey Department of Mechanical Engineering University of Ege 輟mir-35030 Turkey Department of Mechanical Engineering University of Ege Izmir-35030 Turkey

The objective of this study is to examine the effects of cryogenic and aging treatments on the impact strength andmechanical properties of Ti?6Al?4V *** accomplish that objective,cryogenic treatment(CT),aging treatment(AT)andcryogenic treatment followed by aging treatment(CAT)were conducted on Ti?6Al?4V *** tests were performed onheat-treated and untreated samples using different impactor nose geometries(hemispherical,60°and90°conical)to determine theeffect of impactor nose geometry on the damage *** findings showed that energy absorption increased and areas ofdamage decreased as a result of heat treatment in all treated *** highest energy absorption was observed in the CATsamples,due to the increase in energy absorption,the smallest damaged area occurred in the CAT sample,and the largestdeformation was seen in the untreated ***,it was seen that the damaged area and deflection were stronglydependent on impactor nose *** maximum deflection and narrowest deformation area were seen with60o conical *** deformation area increased with increasing impactor nose angle.

关键词： Ti.6Al.4V alloy cryogenic treatment aging treatment low-energy impact test impact damage impactor nose geometry

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