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汉黄芩素减轻LPS诱导BV-2细胞炎症并保护SH-SY5Y细胞的机制

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中国实验方剂学杂志 2024年第20期30卷 62-69页

作者：孙梦菲欧阳竞锋吴春阳程娇娇中国中医科学院医学实验中心中医药防治重大疾病基础研究北京市重点实验室北京100700 北京中医药大学东直门医院北京100007

目的:探讨汉黄芩素对脂多糖(LPS)诱导的BV-2小胶质细胞条件培养基培养SH-SY5Y细胞的保护机制。方法:将BV-2细胞分为空白组、LPS组、汉黄芩素低、中、高浓度组(4、8、16μmol·L^(-1)),其中LPS组给予1 mg·L^(-1)的LPS,汉黄芩素组分别用... 详细信息

目的:探讨汉黄芩素对脂多糖(LPS)诱导的BV-2小胶质细胞条件培养基培养SH-SY5Y细胞的保护机制。方法:将BV-2细胞分为空白组、LPS组、汉黄芩素低、中、高浓度组(4、8、16μmol·L^(-1)),其中LPS组给予1 mg·L^(-1)的LPS,汉黄芩素组分别用相应浓度的汉黄芩素预处理4 h后,给予1 mg·L^(-1)的LPS并使用以上各组的条件培养基培养SH-SY5Y细胞。通过细胞增殖与活性检测(CCK-8)试剂盒检测以上各组BV-2细胞的细胞活力;酶联免疫吸附测定法(ELISA)检测各组BV-2细胞上清液中白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)含量;免疫组织化学法(IHC)检测SH-SY5Y细胞的酪氨酸羟化酶(TH)、α-突触核蛋白(α-Syn)的表达情况;免疫荧光法(IF)检测SH-SY5Y细胞核转录因子-κB p65(NF-κB p65)蛋白核转移和荧光表达强度;蛋白免疫印迹法(Western blot)检测SH-SY5Y细胞Toll样受体4(TLR4)/髓样分化因子88(MyD88)/NF-κB通路相关蛋白表达情况。结果:与空白组比较,LPS组BV-2细胞上清IL-6、TNF-α含量均显著升高(P<0.01);与LPS组比较,汉黄芩素低浓度组BV-2细胞上清IL-6含量降低(P<0.05),汉黄芩素中高浓度组IL-6和TNF-α含量均降低(P<0.05,P<0.01),其中汉黄芩素高浓度组IL-6和TNF-α含量降低最显著(P<0.01),干预效果最佳。与空白组比较,LPS组条件培养基培养的SH-SY5Y细胞α-Syn蛋白表达显著升高、TH蛋白表达显著降低(P<0.01);与LPS组比较,汉黄芩素中高浓度组α-Syn蛋白表达明显降低(P<0.05,P<0.01),汉黄芩素低、中、高浓度组TH蛋白表达明显升高(P<0.05,P<0.01)。与空白组比较,LPS组NF-κB p65蛋白逐渐向核内聚集、荧光表达强度显著增强(P<0.01);与LPS组比较,汉黄芩素各浓度组NF-κB p65蛋白逐渐向核外分散、荧光表达强度逐渐减弱,汉黄芩素高浓度组荧光强度显著减弱(P<0.01)。与空白组比较,LPS组TLR4、磷酸化(p)-NF-κB p65、MyD88蛋白表达明显升高(P<0.05,P<0.01);与LPS组比较,汉黄芩素中浓度组TLR4、MyD88蛋白表达均明显降低(P<0.05,P<0.01);汉黄芩素高浓度组TLR4、p-NF-κB p65、MyD88蛋白表达均明显降低(P<0.05,P<0.01)。结论:汉黄芩素可能通过调控TLR4/MyD88/NF-κB信号通路抑制由LPS诱导的BV-2小胶质细胞炎症因子的释放,保护SH-SY5Y细胞,从而达到减轻炎症和神经保护作用。

关键词：帕金森病汉黄芩素脂多糖(LPS) Toll样受体4(TLR4)信号通路

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从阴火理论论治帕金森病

中医学报 2024年第3期39卷 494-500页

作者：孙梦菲欧阳竞锋丁港中国中医科学院医学实验中心/中医药防治重大疾病基础研究北京市重点实验室北京100700 中国中医科学院西苑医院北京100091

阴火在帕金森病的发生发展中有重要意义,阴火生于五脏,暗耗气血津液,不能奉养周身血脉筋骨以及头部清窍,致使筋脉失养、神明生变,从而出现运动迟缓、肢体僵硬、面具脸、睡眠及精神障碍等症状,并因风助火势,而有震颤麻痹、肢体抖动等表... 详细信息

阴火在帕金森病的发生发展中有重要意义,阴火生于五脏,暗耗气血津液,不能奉养周身血脉筋骨以及头部清窍,致使筋脉失养、神明生变,从而出现运动迟缓、肢体僵硬、面具脸、睡眠及精神障碍等症状,并因风助火势,而有震颤麻痹、肢体抖动等表现。因为五脏不足而现相火、湿郁化火、心火、伏逆之火、风火等诸火皆为阴火之变。肝肾亏虚、脾胃虚弱、心肾不交以及肺主治节的功能失调等病机在帕金森病的产生中具有重要意义;风气内动、风助火势是阴火导致帕金森病的关键因素。临证时应从补肾养肝、培土荣木、交通心肾、调金安木等方面治疗帕金森病。

关键词：阴火帕金森病内伤病

Efficacy of Bushenjianpi prescription on autoimmune premature ovarian failure in mice

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Journal of Traditional Chinese Medicine 2017年第5期37卷 667-674页

作者： Feng Guilin Lin Meijiao Zhou Xiaolin Zhang Lihong Li Jing ouyang jingfeng Gynecological Department of Traditional Chinese Medicine School Clinic Nanyang Normal University College Department of Pediatrics Dongzhimen Hospital Affialited to Beijing University of Chinese Medicine Department of Traditional Chinese Medicine Nanyang Medical College Department of Oncology the Third People'Hospital of Zhengzhou City Department of Traditional Chinese Medicine Children' Hospital of Kaifeng Morphology Laboratory Experimental Research Center China Academy of Chinese Medical Sciences

OBJECTIVE: To assess the efficacy of Bushenjianpi prescription(BSJPP), a formula from Traditional Chinese Medicine, on a mouse model of autoimmune premature ovarian failure(POF) induced by mouse zona pellucida(ZP3) and to investigate the mechanisms underlying the ***: After randomization, POF was induced in the model mice by immunization with ZP3. One week later, mice received low(8.1 mg/kg), moderate(16.2 mg/kg) and high(32.4 mg/kg) doses of BSJPP by gastrogavage once daily for 90 days. Premarin(0.03 mg/kg) served as the positive group. Serum samples were collected 1 week after the last dose and stored at-20 ℃ for analysis. After cervical dislocation, the uterus and ovaries were collected aseptically for evaluation by histological assessment, scanning electron microscopy, immunohistochemical staining, and Western blot and reverse transcription-polymerase chain reaction ***: Serum E2 levels in POF model mice were decreased, whereas follicle-stimulating hormone(FSH) and luteinizing hormone(LH) levels were dramatically increased. Serum levels of E2,LH and FSH were reduced in POF model mice treated with BSJPP(moderate and high doses) and premarin. Anti-bone morphogenetic protein 15(BMP-15) and connexin 43(Cx43) were repressed in autoimmune POF model mice, whereas high expression was observed in control mice and those treated with BSJPP(moderate and high doses)and ***: BSJPP is effective in treating ZP3-induced POF in mice and the increase in the expression of BMP-15 and Cx43 may be implicated in the mechanism underpinning the action.

关键词： Chinese medical formula Primary ovarian Insufficiency Zona pellucida Follicle-stimulating hormone Luteinizing hormone Connexin 43

A new understanding of prescription

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Journal of Traditional Chinese Medicine 2019年第5期39卷 750-752页

作者： Lin Meijiao Liu Ziwang Li Jing Li Hongxing Bian Hua Si Fuchun ouyang jingfeng The Pediatrics Department DongZhiMen HospitalBeijing University of Chinese MedicineBeijing 100700China Department of traditional Chinese medicine Children'Hospital of KaifengKaifengHenan province4750000China Zhang Zhongjing College of Chinese Medicine Nanyang Institute of TechnologyNanyang 473004China Henan Provincial Key Laboratory of Traditional Chinese Medicine Syndrome and Prescription in Signal Transduction Henan Provencial International Joint Laboratory of Traditional Chinese Medicine Syndrome and Prescription in signal transductionHenan University of Chinese MedicineZhengzhouHenan ProvinceP.R.China450046 Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment for Major Diseases Experimental Research CenterChina Academy of Chinese Medical SciencesBeijing 100700China

To promote the development of Traditional Chinese Medicine (TCM), it is necessary to innovate the traditional prescription. It is feasible to use one or several components to substitute TCM, which can be regarded as a process of discarding the dregs and preserving the essential components. In this way, traditional prescription can be converted into various combinations of pharmacological ingredients deriving from several TCMs. Furthermore, some of pharmacological ingredients should be modified to increase their efficacy. It is practical to select the main structural unit with specific substituents having strong pharmacological activity. After the innovation mentioned above, the prescription will evolve into a variety of modified components having distinct pharmacological activity, and this is the novel integration of active ingredients.

关键词： Pharmacology Medicine, Chinese Traditional Combination of pharmacological ingredients Integration of active ingredients

Velvet antler polypeptide is able to induce differentiation of neural stem cells towards neurons in vitro

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Journal of Traditional Chinese Medicine 2017年第3期37卷 308-313页

作者： Zhang Lihong Zhuang Zhihong Sun Yanan Ma Shuhua Yang Weifeng Lei Hongtao Zuo Junling ouyang jingfeng Wang Yi Graduate School Guangzhou University of Chinese MedicineGuangzhou 510006China Morphological laboratory Expelmental Research CenterResearch and Development Service BaseChina Academy of Chinese Medical SciencesBeijing 100700China First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine Guangzhou 510405China

OBJECTIVE: To investigate the neural differentiation capacity of water extraction of velvet ***: Velvet antler(Cervus Nippon Temminck) polypeptide(VAP) was used to differentiate neural stem cells(NSCs) towards neurons in the study. Firstly, we obtain the polypeptides of VAP by water extraction. Secondly, we observed the morphology, assayed the factors in the media by enzyme-linked immunosorbent assay, and detected the special neural molecules by immuno fl uorescence staining. NSCs were cultured on the cell climbing film. After neuronal differentiation, differentiated NSCs were mounted for immunocytochemistry with immunofluorescence ***: The differentiating cells look like neuron,some special factors, such as Glial cell line-derived neurotrophic factor, nerve growth factor, in the media can be detected while differentiated neuron-like cells can express the special neural ***: Differentiation of NSCs towards neurons can be induced by velvet antler polypeptide.

关键词： line-derivedCornu cervi pantotrichum Glial cell neurotrophic factors, Nerve growthfactor Neural stem cells

Effect of Cyclocarya paliurus polysaccharides on streptozotocin-induced diabetic nephropathy in rats

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Journal of Traditional Chinese Medicine 2020年第6期40卷 956-964页

作者： Xia Xichao Mao Dongxue Dai Hongmei Wu Xi Zhang Zuyuan Wang Huaying Zhou Wenwen Dong Yanmei Wang Mengqi Li Yuan Shao Xiangyang ouyang jingfeng Medical College Pingdingshan UniversityPingdingshan 467000China Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment for Major Diseases Experimental Research CenterChina Academy of Chinese Medical SciencesBeijing 100700China

OBJEVTIVE:To investigate the efficacy of Cyclocarya paliurus(***)polysaccharides on streptozotocin-induced diabetic nephropathy in ***:Rats were divided into 6 groups,including group of normal control,group of diabetic control,group of metformin treatment,low-dose group of *** polysaccharides treatment,middle-dose group of *** polysaccharides treatment and high-dose group of *** polysaccharides *** analysis of kidney was analyzed using hematoxilin and *** of blood glucose,creatinine,urea,uric acid were determined by ***-oxidative enzymes were measured by real-time polymerase chain reaction(PCR)and enzyme-linked immunosorbent assay(ELISA).Advanced glycation end products(AGEs)and transforming growth factor-β1(TGF-β1)level was measured by ***:Abnormal changes were observed in the group of diabetic control characterized by atrophy of the renal glomeruli with hypercellularity,congestion of glomerular tufts,dilation of the renal spaces,and degeneration of renal *** with that of normal group,blood glucose,creatinine,urea,uric acid level was significantly increased in the group of diabetic *** dismutase,catalase,glutathione peroxidase,glutathione reductase level was significantly decreased,but AGEs and TGF-β1 level was significantly *** contrast,administration of *** polysaccharides and metformin could reverse the above-mentioned results of the group of diabetic control,especially in the high-dose group of *** ***:Our findings suggest that *** polysaccharides may play a protecting role for nephropathy of diabetic rats by lowering glucose,creatinine,urea,uric acid level,enhancing the antioxidative ability,and reducing AGEs and TGF-β1 expression.

关键词： Diabetic nephropathies Cyclocarya paliurus Polysaccharides Antioxidatnts Glycation end products advanced Transforming growth factor beta1

Mechanism of Lingbao Huxin Dan(灵宝护心丹)in the treatment of bradyarrhythmia complicated with coronary heart disease:a network pharmacology analysis

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中医杂志:英文版 2023年第5期43卷 1001-1009页

作者： HAN Huagang LI Ziqiang ouyang jingfeng WANG Tianquan DONG Lingyan CAO Junling Department of Traditional Chinese Medicine Nanyang Medical CollegeNanyang 473061China Oncology Department Luoyang Hospital of traditional Chinese MedicineLuoyang 471099China Morphology Laboratory Expeimental Research CenterResearch and Development Service BaseBeijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for Major DiseasesChina Academy of Chinese Medical SciencesBeijing 100700China Pharmaceutical Department Dongfang Hospital of Beijing University of Traditional Chinese MedicineBeijing 100078China Pharmaceutical Department Dongzhimen Hospital of Beijing University of Traditional Chinese MedicineBeijing 100700China

OBJECTIVE:To investigate the mechanism of action of the Lingbao Huxin Dan(灵宝护心丹)in treating bradycardia arrhythmia with coronary heart disease(BACHD)by network ***:The active ingredients of the Lingbao Huxin Dan were screened on the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform and Bioinformatics tools designed for the analysis of molecular mechanisms of Chinese medicine platform;target prediction was conducted with the SwissTargetPrediction database,and Cytoscape 3.8 was used to construct a drug ingredient-target *** Genecards,Online Mendelian Inheritance in Man,and DrugB ank databases were searched for disease *** plots were used to display the common targets of BA-CHD and active *** STRING platform was used to construct a protein-protein interaction *** Metascape data platform was used for Gene Ontology(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis to construct a signaling pathway network of the active ingredients of the Lingbao Huxin ***:There were 121 active ingredients,899 related targets,39 targets important in BA-CHD and 14 targets which intersected between the active ingredients and *** were 27 core therapeutic ingredients,153 biological processes,18 cell ingredients and 20 molecular functions obtained by GO enrichment *** KEGG pathway analysis yielded 19 signaling ***:RBA-CHD may treat BA-CHD by regulating adrenergic receptor beta-1,alpha 1-αadrenergic receptor,calcium voltage-gated channel subunit alpha1 C,alpha-1β-adrenergic receptor,nitric oxide synthase 2,beta-2 adrenergic receptor,voltagedependent calcium channel subunit alpha-2/delta-1,angiotensin-converting enzyme,Raf-1 proto-oncogene serine/threonine-protein kinase,and other targets,potentially by affecting adrenergic receptor binding and calcium channel opening,to regulate the activity of cardiomyocytes.

关键词： bradycardia coronary disease network pharmacology Lingbao Huxin Dan

Antioxidative and immunological effects of Cyclocarya paliurus polysaccharides on the spleen injury of diabetic rats

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Journal of Traditional Chinese Medicine 2021年第5期41卷 739-746页

作者： XIA Xichao LI Bin QIU Ju TIAN Gang CHEN Changdong LA Ming ZHANG Ke QI Jinxu LI Yanyan GAO Huashan SHAO Xiangyang SU Congying WANG Mengqi ouyang jingfeng Medical College Pingdingshan UniversityPingdingshan 467000China College of Chemistry and Chemical Engineering Pingdingshan UniversityPingdingshan 467000China Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment for Major Diseases Experimental Research CenterChina Academy of Chinese Medical SciencesBeijing 100700China

OBJEVTIVE:To investigate the effects of Cyclocarya paliurus(***)polysaccharides on the spleen injury of diabetic ***:Animals were divided into 6 groups,including normal group,model group,control group,low-dose group of *** polysaccharides treatment,middle-dose group of *** polysaccharides treatment and high-dose group of *** polysaccharides *** analysis of spleen was analyzed using hematoxilin and *** of biological parameters and anti-oxidative enzymes were determined by ***-7(IL-7)and IL-10 were measured by enzyme-linked immunosorbent ***:Compared with that of model group,superoxide dismutase,catalase,glutathione peroxidase,glutathione reductase level increased 78.63%(P<0.05),51.76%(P<0.05),2.95 times(P<0.01)and 41.11%(P<0.05)in the high-dose group of *** polysaccharides treatment,***-7 and IL-10 increase 1.66(P<0.01)and 1.21 times(P<0.01)in the high-dose group of *** polysaccharides treatment,***:It is suggested that *** polysaccharides may play a protecting role for spleen injury of diabetic rats by enhancing the antioxidative ability and evaluating the immunity.

关键词： spleen diabetes mellitus antioxidants polysaccharides Cyclocarya paliurus

人口红利窗口关闭期体育用品业发展困境及路径选择

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首都体育学院学报 2016年第5期28卷 387-391,401页

作者：邹月辉欧阳井凤大连理工大学文法学院辽宁盘锦124000

运用文献资料法分析我国体育用品业在"人口红利窗口关闭期"的发展困境,认为随着人口红利窗口关闭期的到来,体育用品业廉价劳动力优势丧失,企业缺乏创新所导致的竞争不足等问题也开始暴露,整个产业发展出现暂歇。而要摆脱这种困局实现产... 详细信息

运用文献资料法分析我国体育用品业在"人口红利窗口关闭期"的发展困境,认为随着人口红利窗口关闭期的到来,体育用品业廉价劳动力优势丧失,企业缺乏创新所导致的竞争不足等问题也开始暴露,整个产业发展出现暂歇。而要摆脱这种困局实现产业"二次增长"就应加快人力资本积累提高生产效率,挖掘人才红利;推动产业结构调整和转变增长模式,提升技术红利;通过产业细分和整合销售渠道等措施刺激消费,充分开发体育用品业发展的"第二次人口红利"。

关键词：人口红利体育用品业发展困境路径选择人力资本人才红利整合销售渠道