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Clean Monascus pigments production from Chinese rice wine wastes through submerged fermentation

Food Bioscience 2023年第2期52卷 843-850页

作者： Mengfei Long Yongchao Cai Nan Zheng Zhi Lu Wentao Cao Yaxian Li Xiaomei Pei osire tolbert Xiaole Xia The Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi214122China Infinitus(China)Co.Ltd. Guangzhou510665China Faculty of Biology Shenzhen MSU-BIT UniversityShenzhen518172China

The potential for Monascus pigments(MPs)production in a mixture of Chinese rice wine lees,soaked rice wastewater and broken rice was *** production reached 120.1 U/mL with optimal solid-liquid ratio of culture medium 1:10 and 20 g/L broken rice addition at shake flask *** further explored the effects of stirring speed on MPs production in 7.5 L agitated *** stirring speed had a great impact on cell morphology and the maximum MPs concentration and yield were 128.4 U/mL and 8174 U/g respectively at 400 *** soluble chemical oxygen demand for the soaked rice wastewater decreased by 78.7%,which was mainly due to the intake of carbohydrates,organic acids and amino ***,a simultaneous fermentation process and production system with two-stage stirring speed control strategy was further developed in the 50 L pilot scale,in which the MPs production reached 158.9 U/***,Chinese rice wine wastes were fully utilized without addition of organic solvents during the process and no generation of new *** results showed that Monascus was capable of converting Chinese rice wine wastes into natural pigments by submerged fermentation,which could provide an economic and environmentally friendly approach to reduce the cost of wastes treatment as well as high-value utilization.

关键词： Monascus pigments Submerged fermentation Chinese rice wine wastes Pilot scale fermentation Simultaneous fermentation process and production system

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Comparative transcriptome analysis reveals metabolic regulation of prodigiosin in Serratia marcescens

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Systems Microbiology and Biomanufacturing 2021年第3期1卷 323-335页

作者： Yang Sun Lijun Wang tolbert osire Weilai Fu Ganfeng Yi Shang-Tian Yang Taowei Yang Zhiming Rao The Key Laboratory of Industrial Biotechnology School of BiotechnologyJiangnan UniversityMinistry of Education1800 Lihu AvenueWuxiJiangsu 214122P.R.China Fujian Dabeinong Aquatic Sci.&Tech.Co. Ltd.Zhangzhou 363500FujianChina Department of Chemical and Biomolecular Engineering The Ohio State UniversityColumbusOH 43210USA

Prodigiosin is a secondary metabolite mainly produced at 30°C in Serratia marcescens,but it can hardly be synthetized at 37°C or *** this study,we provide insight into the metabolic regulation of prodigiosin synthesis in response to temperature through transcriptome *** analysis of the function of differentially expressed genes suggested that temperature resulted in significant alteration of the metabolic pathways between 30 and 37°***,30°C favored transcriptional expression of the pig gene *** the same time,the carbon flux was redistributed to pathways of pyruvate,proline,serine,especially homoserine,cystathionine,homocysteine,methionine,and s-adenosylmethionine metabolism,all involved in prodigiosin biosynthesis,and was finally increased towards the prodigiosin synthesis pathway in *** at 30°***,results further confirmed increased transcriptional level of five regulators(LuxS,RpoS,Hfq,EepR,CRP),and decreased content of hexS through ***,successful co-overexpression of mmuM and metK,related to homocysteine,methionine,and s-adenosylmethionine metabolism,in the chromosome of JNB5-1(JNB5-1/MK)resulted in increased prodigiosin titer up to 7.57 g/L in JNB5-1/MK at 30°C,which was 41.2%higher than that in *** transcriptome analysis provides further insight into the strain’s response to temperature changes at the transcription level,which is of great significance for improving the production of prodigiosin.

关键词： S.marcescens Prodigiosin Transcriptome Temperature-dependent Metabolic regulation

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赖氨酸脱羧酶分子改造及其催化合成戊二胺

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食品与发酵工业 2022年第1期48卷 8-14页

作者： osire tolbert 杨套伟乔郅钠孙杨徐美娟张显邵明龙饶志明江南大学生物工程学院江苏无锡214122

1,5-戊二胺(戊二胺)具有良好的生物活性,广泛应用在农业、医药以及工业等领域。赖氨酸脱羧酶可以催化L-赖氨酸生产戊二胺,为了提高赖氨酸脱羧酶催化合成戊二胺的效率,首先在大肠杆菌中克隆表达了粘质沙雷氏菌来源的赖氨酸脱羧酶(SmcadA... 详细信息

1,5-戊二胺(戊二胺)具有良好的生物活性,广泛应用在农业、医药以及工业等领域。赖氨酸脱羧酶可以催化L-赖氨酸生产戊二胺,为了提高赖氨酸脱羧酶催化合成戊二胺的效率,首先在大肠杆菌中克隆表达了粘质沙雷氏菌来源的赖氨酸脱羧酶(SmcadA)。生化特征表明,SmcadA最适催化pH为6.0,最适催化温度约为40℃。随后对SmcadA的第348位氨基酸进行了突变研究,筛选获得了催化效率显著提高的突变体Gly348Ala,主要原因是该突变导致蛋白中氨基酸残基和底物之间相互作用的氢键数增加,从而影响其催化效率。最后,对重组菌株细胞进行了戊二胺合成研究,催化反应25 h,含有突变体Gly348Ala的重组菌细胞可以催化合成218.2 g/L戊二胺,野生型SmcadA重组菌仅能催化合成159.2 g/L戊二胺。该研究结果为工业化酶催化合成戊二胺提供了借鉴。

关键词：赖氨酸脱羧酶分子改造氢键酶催化戊二胺

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