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High fat diet dysregulates microRNA-17-5p and triggers retinal inflammation:Role of endoplasmic-reticulum-stress

World Journal of Diabetes 2017年第2期8卷 56-65页

作者： maha Coucha Islam N mohamed Sally L Elshaer osinakachuk m.ata megan L Bartasis Azza B El-Remessy Clinical and Experimental Therapeutics College of PharmacyUniversity of GeorgiaAugustaGA 30912United States

AIm To elucidate how high diet-induced endoplasmic reticulum-stress upregulates thioredoxin interacting protein expression in müller cells leading to retinal inflammation. mETHODS male C57Bl/J mice were fed either normal diet or 60% high fat diet for 4-8 wk. During the 4 wk study, mice received phenyl-butyric acid(PBA); endoplasmic reticulum-stress inhibitor; for 2 wk. Insulin resistance was assessed by oral glucose tolerance. Effects of palmitate-bovine serum albumin(BSA)(400 μmol/L) were examined in retinal müller glial cell line and primary müller cells isolated from wild type and thioredoxin interacting protein knock-out mice. Expression of thioredoxin interacting protein, endoplasmic reticulum-stress markers, mi R-17-5p m RNA, as well as nucleotide-binding oligomerization domain-like receptor protein(NLRP3) and IL1β protein was *** High fat diet for 8 wk induced obesity and insulin resistance evident by increases in body weight and impaired glucose tolerance. By performing quantitative real-time polymerase chain reaction, we found that high fat diet triggered the expression of retinal endoplasmic reticulum-stress markers(P mi R-17-5p expression, whichwere restored by inhibiting endoplasmic reticulumstress with PBA(P mitate-BSA triggered endoplasmic reticulum-stress markers, which was accompanied with reduced mi R-17-5p and induced thioredoxin interacting protein m RNA in retinal müller glial cell line(P mitate upregulated NLRP3 and IL1β expression in primary müller cells isolated from wild type. However, using primary müller cells isolated from thioredoxin interacting protein knock-out mice abolished palmitate-mediated increase in NLRP3 and IL1β.CONCLUSION Our work suggests that targeting endoplasmic reticulumstress or thioredoxin interacting protein are potential therapeutic strategies for early intervention of ob

关键词： High fat diet Palmitate Endoplasmic-reticulum-stress Inflammation Thioredoxin-interacting protein micro-RNA 17-5p

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