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A facile strategy for tuning the density of surface-grafted biomolecules for melt extrusion-based additive manufacturing applications

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Bio-Design and Manufacturing 2024年第3期7卷 277-291页

作者： I.A.O.Beeren G.dos santos P.J.Dijkstra C.Mota J.Bauer H.Ferreira Rui l.Reis N.Neves S.Camarero-Espinosa M.B.Baker l.Moroni Department of Complex Tissue Regeneration MERLN Institute for Technology-Inspired Regenerative MedicineMaastricht University6229 ER MaastrichtThe Netherlands 3B’s Research Group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal ICVS/3B’s-PT Government Associate laboratory 4806-909 Braga/GuimarãesPortugal POlYMAT University of the Basque Country UPV/EHU20018 Donostia/San SebastiánSpain IKERBASQUE Basque Foundation for Science48009 BilbaoSpain

Melt extrusion-based additive manufacturing(ME-AM)is a promising technique to fabricate porous scaffolds for tissue engi-neering ***,most synthetic semicrystalline polymers do not possess the intrinsic biological activity required to control cell *** of biomolecules on polymeric surfaces of AM scaffolds enhances the bioactivity of a construct;however,there are limited strategies available to control the surface ***,we report a strategy to tune the surface density of bioactive groups by blending a low molecular weight poly(ε-caprolactone)5k(PCl5k)containing orthogonally reactive azide groups with an unfunctionalized high molecular weight PCl75k at different *** porous three-dimensional(3D)scaf-folds were then fabricated using a high weight percentage(75 wt.%)of the low molecular weight PCl *** a proof-of-concept test,we prepared films of three different mass ratios of low and high molecular weight polymers with a thermopress and reacted with an alkynated fluorescent model compound on the surface,yielding a density of 201-561 pmol/cm^(2).Subsequently,a bone morphogenetic protein 2(BMP-2)-derived peptide was grafted onto the films comprising different blend compositions,and the effect of peptide surface density on the osteogenic differentiation of human mesenchymal stromal cells(hMSCs)was *** two weeks of culturing in a basic medium,cells expressed higher levels of BMP receptor II(BMPRII)on films with the conjugated *** addition,we found that alkaline phosphatase activity was only significantly enhanced on films contain-ing the highest peptide density(i.e.,561 pmol/cm^(2)),indicating the importance of the surface *** together,these results emphasize that the density of surface peptides on cell differentiation must be considered at the cell-material ***,we have presented a viable strategy for ME-AM community that desires to tune the bulk and surface functionality via blending of(m

关键词： Additive manufacturing Blending Surface functionalization Surface density Click chemistry Human

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An oxygenating colloidal bioink for the engineering of biomimetic tissue constructs

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Bio-Design and Manufacturing 2024年第3期7卷 240-261页

作者： Seol-Ha Jeong Jarno Hiemstra Patrick V.Blokzijl Rebeca Damian-Ferrara Danilo Martins dos santos Jéssica H.l.da Fonseca Min-Ho Kang Jihyun Kim Dilara Yilmaz-Aykut Mei l.l.Cham-Pérez Jeroen leijten Su Ryon Shin Division of Engineering in Medicine Department of MedicineBrigham and Women’s HospitalHarvard Medical SchoolBostonMA 02139USA leijten lab Department of Developmental BioEngineeringFaculty of Science and TechnologyTechnical Medical CentreUniversity of TwenteDrienerlolaan 57522 NB EnschedeThe Netherlands Nanotechnology National laboratory for Agriculture(lNNA) Embrapa InstrumentaçãoSao CarlosSao Paulo 13560-970Brazil Department of Biomedical-Chemical Engineering The Catholic University of Korea43 Jibong-roBucheon-siGyeonggi-do 14662Republic of Korea Department of Biotechnology The Catholic University of Korea43 Jibong-roBucheon-siGyeonggi-do 14662Republic of Korea

Ensuring a sufficient oxygen supply is pivotal for the success of bioprinting applications since it fosters tissue integration and natural *** in oxygen concentration among diverse tissues necessitates the precise recreation of tissue-specific oxygen levels in imprinted constructs to support the survival of targeted *** oxygen-releasing biomaterials,such as oxygen-generating microparticles(OMPs),have shown promise for enhancing the oxygen supply of microenvironments in injured tissues,whether this approach is scalable for large tissues and whether tissue-specific bioinks with varying OMP concentrations remain printable remain *** study addresses this critical gap by introducing an innovative class of engineered oxygenated bioinks that combine colloidal-based microgels with *** report that incorporating nanosized calcium peroxide(nCaO_(2))and manganese oxide nanosheets(nMnO_(2))into hydrophobic polymeric microparticles enables precise modulation of oxygen release while controlling hydrogen peroxide ***,the fabrication of oxygenating and cytocompatible colloidal gels is achieved using an aqueous two-phase *** study thoroughly evaluates the fundamental characteristics of the resulting bioink,including its rheological behaviors,printability,shape fidelity,mechanical properties,and oxygen release ***,this study demonstrates the macroscopic scalability and cytocompatibility of printed constructs produced via cell-laden oxygenating colloidal *** showcasing the effectiveness of extrusion-based bioprinting,this study underscores how it can be used to fabricate biomimetic tissues,indicating its potential for new *** findings presented here advance the bioprinting field by achieving scalability with both high cell viability and the possibility of mimicking specifically oxygenated *** work thereby offers a promising avenue for the development of functional tissues with e

关键词： 3D bioprinting Bioink Colloidal gels Extrusion printing Oxygen-generating microparticle

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Emerging role of liquid biopsy in rat sarcoma virus mutated metastatic colorectal cancer:A case report

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World Journal of Gastrointestinal Oncology 2024年第1期16卷 234-243页

作者： João Gramaça Isabel Gomes Fernandes Carolina Trabulo Joana Gonçalves Rita Gameiro dos santos Adriano Baptista Idília Pina Centro Hospitalar Barreiro Montijo Centro Hospitalar Barreiro MontijoSetúbalBarreiro 2830-003Portugal Medical Oncology Unit Centro Hospitalar Barreiro MontijoSetúbalBarreiro 2830-003Portugal

BACKGROUND In patients with metastatic colorectal cancer(mCRC),the treatment options are limited and have been proved to be affected by rat sarcoma virus(RAS)mutational *** RAS wild-type(wt)patients,the combination of antiepidermal growth factor receptor(EGFR)monoclonal antibodies with chemotherapy(CT)is more effective than CT *** the other hand,RAS-mutated patients are not eligible for treatment with anti-EGFR *** SUMMARY Eleven patients with initially RAS-mutated mCRC were followed from diagnosis to May *** the time of cell-free DNA determination,five patients had undergone one CT line,five patients had undergone two CT lines,and one patient had undergone three CT lines(all in combination with bevacizumab).At the second and third treatment lines[second line(2l),third line(3l)],patients with neo-RAS wt received a combination of CT and *** neo-RAS wt patients treated with anti-EGFR,our findings indicated an increase in progression-free survival for both 2l and 3l(14.5 mo,P=0.119 and 3.9 mo,P=0.882,respectively).Regarding 2l overall survival,we registered a slight increase in neo-RAS wt patients treated with anti-EGFR(33.6 mo vs 32.4 mo,P=0.385).At data cut-off,two patients were still alive:A RAS-mutated patient undergoing 3l treatment and a neo-RAS wt patient who received 2l treatment with anti-EGFR(ongoing).CONClUSION Our case series demonstrated that monitoring RAS mutations in mCRC by liquid biopsy may provide an additional treatment line for neo-RAS wt patients.

关键词： Metastatic colorectal cancer Rat sarcoma virus mutational status liquid biopsy Rat sarcoma virus wild-type Neo-rat sarcoma virus wild-type Anti-epidermal growth factor receptor therapy Case report

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Application of novel constrained friction processing method to produce fine grained biomedical Mg-Zn-Ca alloy

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Journal of Magnesium and Alloys 2024年第2期12卷 516-529页

作者： Ting Chen Banglong Fu Junjun Shen Uceu F.H.R.Suhuddin Björn Wiese Yuanding Huang Min Wang Jorge F.dos santos Jean Pierre Bergmann Benjamin Klusemann Solid State Materials Processing Institute of Material MechanicsHelmholtz-Zentrum HereonMax-Planck-Str.1Geesthacht 21502Germany Functional Magnesium Materials Institute of Metallic BiomaterialsHelmholtz-Zentrum HereonMax-Planck-Str.1Geesthacht 21502Germany Shanghai Key laboratory of Materials laser Processing and Modification Shanghai Jiao Tong UniversityDongchuan Road 800Shanghai 200240China Applied Materials and Manufacturing Energy and Environment DivisionPacific Northwest National LaboratoryPO Box 999RichlandWA 99352USA Production Technology Group Technisiche Universität IlmenauGustav-Kirchhoff-Platz 2Ilmenau 98693Germany Institute for Production Technology and Systems Leuphana University of LüneburgUniversitätsallee 1Lüneburg 21335Germany

In order to obtain Mg alloys with fine microstructures and high mechanical performances,a novel friction-based processing method,name as“constrained friction processing(CFP)”,was *** CFP,defect-free Mg-Zn-Ca rods with greatly refined grains and high mechanical properties were *** to the previous as-cast microstructure,the grain size was reduced from more than 1 mm to around 4μm within 3 s by a single process *** compressive yield strength was increased by 350%while the ultimate compressive strength by 53%.According to the established material flow behaviors by“tracer material”,the plastic material was transported by shear *** the base material to the rod,the material experienced three stages,*** by the tool,upward flow with additional tilt,followed by upward *** microstructural evolution was revealed by“stop-action”*** microstructural development at regions adjacent to the rod is mainly controlled by twinning,dynamic recrystallization(DRX)as well as particle stimulated nucleation,while that within the rod is related to DRX combined with grain growth.

关键词： Constrained friction processing Magnesium alloys Microstructure Mechanical properties Grain refinement Plastic deformation

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Exploring the Factors Associated with 12-Month Non-Return to Work among Motorcyclists Involved in Road Accidents

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Open Journal of Preventive Medicine 2024年第1期14卷 1-16页

作者： Yolaine Glèlè-Ahanhanzo Donatien Daddah Alphonse Kpozehouen Bella Hounkpè dos santos Moussiliou N. Paraiso Multidisciplinary Research Unity for Road Crashes Prevention (ReMPARt) Department of Epidemiology and Bio-Statistics Regional Institute of Public Health University of Abomey-Calavi Ouidah Benin Regional Direction of Health Lokossa Benin Regional Direction of Health Abomey-Calavi Benin Health Promotion Department Regional Institute of Public Health Ouidah Benin

Introduction: Motorcyclists bear a disproportionate burden of morbidity and mortality from road accidents. In addition, the consequences of these accidents affect the ability of victims to return to work. This study aimed to determine the prevalence and factors associated with non-return to work among surviving motorcyclists involved in road accidents 12 months after the event. Materials and Methods: It was a cross-sectional study conducted using data from a cohort of motorcyclists involved in accidents and recruited in five hospitals in Benin from July 2019 to January 2020. The dependent variable was non-return to work 12 months after the accident (yes vs no). The independent variables were categorized into two groups: baseline and 12-month follow-up variables. logistic regression was used to determine the factors associated with non-return to work at 12 months among the participants. Results: Among the 362 participants, 55 (15.19%, 95% CI = 11.84 - 19.29) had not returned to work 12 months after the accident. Risk factors for non-return to work identified were: smoking (aOR = 4.41, 95% CI = 1.44 - 13.56, p = 0.010), hospitalization (aOR = 2.87, 95% CI = 1.14 - 7.24, p Conclusion: The prevalence of non-return to work at 12 months was high among surviving motorcyclists involved in road accidents in Benin. Integrated support for patients based on identified risk factors should effectively improve their return to work.

关键词： Road Accident Return to Work Motorcyclists Hospital Mental Health Cohort Benin

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Boll weevils in dry bolls:is there a performance disadvantage?

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Journal of Cotton Research 2024年第3期7卷 243-252页

作者： COElHO Beatriz S. lEITE Suzany A. santos Mateus P.dos MATES Eloito C.de MORAES Beatriz M.l.de BASTOS Cristina S. GUEDES Raul N.C. MOREIRA Aldenise A. CASTEllANI Maria A. Departamento de Fitotecnia e Zootecnia Universidade Estadual do Sudoeste da BahiaVitória da ConquistaBA 45031‑900Brazil Faculdade de Agronomia e Veterinária Universidade de BrasíliaBrasíliaDF 70910‑900Brazil Departamento de Entomologia Universidade Federal de ViçosaViçosaMG 36570‑900Brazil Pós‑GraduaCão em Agronomia(Fitotecnia) Universidade Estadual do Sudoeste da BahiaVitória da ConquistaBA 45031‑900Brazil

Background Anthonomus grandis grandis is the main cotton pest in the Americas,with the potential to become a pest in other *** insect uses the plant's reproductive organs for feeding,oviposition,and survival strategies in the off-season,sheltering in dry *** study aimed to know the reproductive potential,feeding,longevity,and morphometric characteristics of boll weevils from dry bolls,squares,and *** were obtained from infested squares,dry bolls,and traps installed in cotton fields and the Caatinga *** weevil pairs were maintained in the laboratory to assess the oviposition,feeding holes,longevity,and morphometric measurements of the parental and F_(1) *** In the parental generation,boll weevils from traps(the Caatinga area and cotton cultivation)showed greater reproductive *** weevils from squares and the Caatinga area showed greater feeding activity compared with those from cotton *** rostrum length,thorax+abdomen length,and abdomen width were observed in boll weevils from cotton cultivation and dry *** origin of boll weevils parental generation did not affect progeny emergence *** F_(1) generation of boll weevils showed significant differences in average longevity depending on the origin of different ***,differences in rostrum lengths were observed,with the boll weevils coming from traps installed in cotton cultivation,the Caatinga area,and dry bolls being the *** thorax+abdomen length and abdomen width,the highest values were observed in those from the Caatinga area and cotton *** It was proven that dry bolls did not affect the reproductive,feeding,and longevity performance of boll weevils,enabling better insect fitness(reproduction and size)in relation to those coming from *** use of dry bolls as off-season shelter is an important survival strategy for the boll weevil.

关键词： Anthonomus grandis grandis Gossypium hirsutum Off-season Survival

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Building Expertise on FAIR Through Evolving Bring Your Own Data(BYOD) Workshops: Describing the Data, Software, and Management-focused Approaches and Their Evolution

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Data Intelligence 2024年第2期6卷 429-456页

作者： César H.Bernabé lieze Thielemans Rajaram Kaliyaperumal Claudio Carta Shuxin Zhang Celia W.G.van Gelder Nirupama Benis luiz Olavo Bonino da Silva santos Ronald Cornet Bruna dos santos Vieira Nawel lalout Ines Henriques Alberto Camara Ballesteros Kees Burger Martijn G.Kersloot Friederike Ehrhart Esther van Enckevort Chris T.Evelo Alasdair J.G.Gray Marc Hanauer Kristina Hettne Joep de ligt Arnaldo Pereira Nuria Queralt-Rosinach Erik Schultes Domenica Taruscio Andra Waagmeester Mark D.Wilkinson Egon l.Willighagen Mascha Jansen Barend Mons Marco Roos Annika Jacobsen Biosemantics Group Department of Human GeneticsLeiden University Medical CentreLeidenThe Netherlands NIHR Academic Fellow in General Practice University College London and The Royal Free NHS Foundation Trust National Centre for Rare Diseases Istituto Superiore di SanitaRomeItaly Department of Medical Informatics Amsterdam UMC location University of AmsterdamMeibergdreef 9AmsterdamThe Netherlands Amsterdam Public Health Methodology&Digital HealthAmsterdamThe Netherlands Health-Rl UtrechtThe Netherlands Semantics Services and Cybersecurity groupUniversity of TwenteEnschedeThe Netherlands Department of Medical Imaging Radboud Institute for Health SciencesRadboud University Medical CenterNijmegenThe Netherlands Department of Medical BioSciences Radboud University Medical CenterNijmegenThe Netherlands The Duchenne Parent Project VeneendaalThe Netherlands Departamento de Biotecnologia-Biologia Vegetal Escuela Tecnica Superior de Ingenieria AgronomicaAlimentaria y de BiosistemasCentro de Biotecnologia y Genomica de PlantasUniversidad Politecnica de Madrid(UPM)-Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria-CSIC(INIA-CSIC).Campus MontegancedoPozuelo de Alarcon(Madrid)Spain Castor AmsterdamThe Netherlands Department of Bioinformatics-BiGCaT Maastricht UniversityMaastrichtThe Netherlands University Medical Center Groningen University of GroningenGroningenThe Netherlands Maastricht Centre for Systems Biology(MaCSBio) Maastricht UniversityMaastrichtThe Netherlands Department of Computer Science Heriot-Watt UniversityEdinburghUK INSERM US14-OrphanetPlateforme Maladies RaresParisFrance Centre for Digital Scholarship Leiden University LibrariesThe Netherlands Center for Molecular Medicine Cancer Genomics NetherlandsDepartment of GeneticsUniversity Medical Center UtrechtUniversity UtrechtThe Netherlands University of Aveiro DETI/IEETAAveiroPortugal GO FAIR Foundation LeidenThe Netherlands The leiden Academic Center for Drug Research LeidenThe Netherlands Micel

Since 2014,"Bring Your Own Data"workshops(BYODs)have been organised to inform people about the process and benefits of making resources Findable,Accessible,Interoperable,and Reusable(FAIR,and the FAIRification process).The BYOD workshops'content and format differ depending on their goal,context,and the background and needs of ***-focused BYODs educate domain experts on how to make their data FAIR to find new answers to research ***-focused BYODs promote the benefits of making data FAIR and instruct project managers and policy-makers on the characteristics of FAIRification ***-focused BYODs gather software developers and experts on FAIR to implement or improve software resources that are used to support ***,these BYODs intend to foster collaboration between different types of stakeholders involved in data management,curation,and reuse(*** experts,trainers,developers,data owners,data analysts,FAIR experts).The BYODs also serve as an opportunity to learn what kind of support for FAIRification is needed from different communities and to develop teaching materials based on practical examples and *** this paper,we detail the three different structures of the BYODs and describe examples of early BYODs related to plant breeding data,and rare disease registries and biobanks,which have shaped the structure of the *** discuss the latest insights into making BYODs more productive by leveraging our almost ten years of training experience in these workshops,including successes and encountered ***,we examine how the participants'feedback has motivated the research on FAIR,including the development of workflows and software.

关键词： FAIR FAIRification FAIR expertise Bring Your Own Data Workshop BYOD

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Is magnesium deficiency the major cause of needle chlorosis of Pinus taeda in Brazil?

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Journal of Forestry Research 2024年第2期 213-225页

作者： Ant?nio Carlos Vargas Motta Shizuo Maeda Valdécio dos santos dos santos Rodrigues Tamires Maiara Ercole Stephen Arthur Prior Ana Elisa lyra Brumat Amanda Pacheco Cardoso Moura Julierme Zimmer Barbosa Jo?o Bosco Vasconcellos Gomes Department of Soil Sciences and Agriculture Engineering Federal University of Paraná Brazilian Agricultural Research Company Embrapa Florestas 3. United States Department of Agriculture-Agricultural Research Service USDA-ARS National Soil Dynamics Laboratory 4. Reforestation Remasa 5. Federal Institute of Southeast of Minas Gerais

Needle chlorosis(NC) in Pinus taeda l. systems in Brazil becomes more frequent after second and third harvest rotation cycles. In a study to identify factors contributing to yellowing needle chorosis(YNC), trees were grown in soils originating from contrasting parent materials, and soils and needles(whole, green and chlorotic portions) from 1-and 2-year-old branches and the first and second needle flush release at four sites with YNC on P. taeda were analyzed for various elements and properties. All soils had very low base levels(Ca2+, Mg2+and K+) and P, suggesting a possible lack of multiple elements. YNC symptoms started at needle tips, then extended toward the needle base with time. First flush needles had longer portions with YNC than second flush needles did. Needles from the lower crown also had more symptoms along their length than those higher in the canopy. Symptoms were similar to those reported for Mg. In chlorotic portions, Mg and Ca concentrations were well below critical values; in particular, Mg levels were only one third of the critical value of 0.3 g kg-1. Collectively, results suggest that Mg deficiency is the primary reason for NC of P. taeda in various parent soils in Brazil.

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Natural and Eco-Friendly Materials for Triboelectric Energy Harvesting

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Nano-Micro letters 2020年第3期12卷 179-196页

作者： Vladislav Slabov Svitlana Kopyl Marco PSoares dos santos Andrei l.Kholkin Department of Physics and CICECO‑Aveiro Institute of Materials University of Aveiro3810‑193 AveiroPortugal Centre for Mechanical Technology and Automation(TEMA) University of Aveiro3810‑193 AveiroPortugal Department of Mechanical Engineering University of Aveiro3810‑193 AveiroPortugal School of Natural Sciences and Mathematics Ural Federal UniversityEkaterinburgRussia 620000 laboratory of Functional low‑Dimensional Structures National University of Science and Technology MISiSMoscowRussia 119049

Triboelectric nanogenerators(TENGs)are promising electric energy harvesting devices as they can produce renewable clean energy using mechanical excitations from the *** designs of triboelectric energy harvesters relying on biocompatible and eco-friendly natural materials have been introduced in recent *** ability to provide customizable self-powering for a wide range of applications,including biomedical devices,pressure and chemical sensors,and battery charging appliances,has been *** review summarizes major advances already achieved in the field of triboelectric energy harvesting using biocompatible and eco-friendly natural materials.A rigorous,comparative,and critical analysis of preparation and testing methods is also *** power up to 14 mW was already achieved for the dry leaf/polyvinylidene fluoride-based TENG *** findings highlight the potential of eco-friendly self-powering systems and demonstrate the unique properties of the plants to generate electric energy for multiple applications.

关键词： Natural and eco-friendly materials Energy harvesting Triboelectric nanogenerators Biocompatibility

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Fertilization of Tifton 85 with Swine liquid Manure

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Agricultural Sciences 2019年第7期10卷 918-926页

作者： Marinho Rocho da Silva Joadil Goncalves de Abreu oscarlina l.cia dos santos weber Flabiele Soares da Silva Edna Maria Bonfim-Silva Ana Paula Alves Barreto Damasceno Faculty of Agronomy and Animal Science Federal University of Mato GrossoCuiabáBrasil Department of Agricultural and Environmental Engineering Institute of Agricultural Sciences and TechnologyFederal University of Mato GrossoRondonópolisBrazil

The objective was to verify the possibility of supplying the nutritional requirement of Tifton 85 grass with swine liquid manure in an intensive management system. The experiment was carried out in a randomized block design, with four treatments of swine liquid manure doses: 0, 70, 140 and 210 m3·ha-1, divided into seven applications and a treatment of mineral fertilization of 200 kg of nitrogen ha-1·year-1. Samples of the collected plant material were weighed to obtain fresh mass taken for drying and then ground for bromatological determination. The dry mass production data were obtained by dry matter accumulation during the cuts and averages were made for the variables;plant height, crude protein, NDF, ADF, neutral detergent insoluble protein (NDIP) and acid detergent insoluble protein (ADIP). The data were subjected to variance analysis, performing regression for swine liquid manure doses and averages test (Dunnett test) to compare the doses with the mineral fertilization. Higher plant heights, dry mass production, crude protein content, neutral detergent insoluble protein content and lower neutral detergent fiber content in Tifton 85 grass were observed with mineral fertilization. In the variables, acid detergent fiber and acid detergent insoluble protein there was no difference (P = 0.05) between the mineral fertilization and the swine liquid manure doses. There was a linear increase (P = 0.05) of swine liquid manure doses only in dry matter production. Swine liquid manure doses up to 210 m3·ha-1·year-1 do not meet the entire nutritional requirement of Tifton 85 grass, recommending the evaluation of higher swine liquid manure doses or complementation with mineral fertilization.

关键词： Bromatology Cynodon spp. Organic Fertilization Waste Reuse

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