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<i>In Vitro</i>Antiprotozoal and Cytotoxic Activity of the Aqueous Extract, the 80% Methanol Extract and Its Fractions from the Seeds of <i>Brucea sumatrana</i>Roxb. (Simaroubaceae) Growing in Democratic Republic of Congo

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Chinese Medicine 2012年第1期3卷 65-71页

作者： Michel Tshodi Ehata Adelard Mbenza Phuati Stanislas Nsaka Lumpu Cyprien Kikweta Munduki Donatien Bakana Phongi Gaston Tona Lutete oscar kambu kabangu Richard Cimanga Kanyanga Ann Matheeussen Paul Cos Sandra Apers Luc Pieters Louis Maes Arnold J. Vlietinck Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa XI Democratic Republic of Congo Laboratory of Microbiology Parasitology and Hygiene (LMPH) Faculty of Pharmaceutical Biomedical and Veterinary Sciences University of Antwerp Antwerp Belgium Laboratory of Microbiology Parasitology and Hygiene (LMPH) Faculty of Pharmaceutical Biomedical and Veterinary Sciences University of Antwerp Antwerp Belgium Laboratory of Pharmacognosy and Pharmaceutical Analysis Department of Pharmaceutical Sciences University of Antwerp Antwerp Belgium

The in vitro antiprotozoal and cytotoxic activity of the aqueous extract, the 80% methanol extract, and its different soluble fractions and subfractions from Brucea sumatrana seeds were assessed against two Trypanosoma (T. cruzi and T. brucei brucei), Leishmania infantum and chloroquine and pyrimethanine-resistant K1strain of P. falciparum and against MRC-5 cell-lines respectively. Results indicated that the 80% methanol extract showed a cytotoxic effect against MRC-5 cell lines with CC50 value of 0.54 μg/ml. It however exhibited pronounced and non selective activity against T. cruzi (IC50 = 1.52 μg/ml, SI = 0.03) and L. infantum (IC50 = 2.41 μg/ml, SI = 0.22). It however displayed pronounced and selective effect against T. brucei brucei (IC50 2.16) and chloroquine and pyrimethamine-resistant K1 strain of P. falciparum (IC50 2.16). All soluble fractions and subfractions from the partition of the 80% methanol extract were found to exhibit an antiprotozoal activity with IC50 values ranging from T .cruzi, T. b. brucei, L. infantum and chloroquine and pyrimethamine-resistant K1 strain of P. falciparum with IC50 values of 0.33, 81, 81 and >81 respectively. The chloroform soluble fraction rich in alkaloid was cytotoxic against MRC-5 cell lines (CC50 = 27.09 μg/ml) and showed good activity against T. b. brucei (IC50 = 8.36 and SI = 3.24) and moderate activity against T. cruzi, L. infantum and chloroquine-pyrimethane-resistant K1 strain of P. falciparum (20 50 50 = 1.55 and 0.43 μg/ml respectively), they however displayed pronounced antiprotozoal activity against T. cruzi, T. b. brucei and chloroquine and pyrimethamine-resistant K1 strain of P. falciparum with IC50 values ranging from P. falciparum (SI = >6.2 and >1.72 respectively). These extracts however showed good and low activity respectively against L. infantum (IC50 = 24.05 and 6.82 μg/ml respectively).

关键词： Brucea sumatrana Simaroubaceae Seeds Antiprotozoal Activity Cytotoxicity

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Study of Antiparasitic and Cytotoxicity of the Aqueous, the 80% Methanol Extract and Its Fractions, and the Acute Toxicity of the Aqueous Extract of Brucea sumatrana (Simaroubaceae) Leaves Collected in Mai-Ndombe, Democratic Republic of Congo

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Chinese Medicine 2016年第3期7卷 93-109页

作者： M. Tshodi Ehata S. Nsaka Lumpu C. Kikweta Munduku O. kambu kabangu P. Cos L. Maes S. Apers A. J. Vlietinck L. Pieters R. Cimanga Kanyanga Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of Congo Faculty of Pharmaceutical Sciences Biomedical and Veterinary Sciences Laboratory of Microbiology Parasitology and Hygiene University of Antwerp Universisteitsplein 1 Antwerpen Belgium Department of Pharmaceutical Sciences Laboratory of Natural Products & Food-Research and Analysis (NatuRa) University of Antwerp Universiteitsplein 1 Antwerpen Belgium

Results from the in vitro evaluation of the antiparasitaire activity of the aqueous extract, the 80% methanol extract and its fractions from the leaves of Brucea sumatrana against Trypanosoma brucei brucei, T. cruzi, Leishmania infantum, the multidrug-resistant K1 and chloroquine-sensitive NF54 strains of Plasmodium falciparum indicated that all samples from the leaves extract presented interesting antiparasitaire activity at different extents. The 80% methanol extract, its chloroform acid, petroleum ether and 80% methanol soluble fractions and the aqueous extract exhibited strong activity against Trypanosoma b. brucei, T. cruzi, L. infantum and the multidrug-resistant K1 strain of P. falciparum with IC50 values from 50 values ranging from 50) 50 was estimated to be greater than 5 g/kg. In addition, it did not significantly modify the concentration levels of some evaluated biochemical and hematological parameters in treated rats. These results constitute a scientific validation supporting and justifying the traditional use of the leaves of B. sumatrana for the treatment of malaria, sleeping sickness and at some extent Chagas disease.

关键词： Brucea sumatrana Simaroubaceae Leaves Extracts Antiprotozoal Activity Cyto-toxic Activity Acute Toxicity

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