检索结果-南通市图书馆

检索条件"作者=Oscar CASTILLO"

共 11 条记录，以下是11-20 订阅

全选清除本页清除全部题录导出标记到"检索档案"

详细简洁

排序：

Fluorescein derivatives potentiate CFTR by altering the ATP affinity and efficacy of channel gating

Fluorescein derivatives potentiate CFTR by altering the ATP ...

引用

2008北京国际生理科学学术大会

作者： MORAN oscar SHEPPARD David N Istituto di Biofisica CNRVia DeMarini 616149 GenoaItaly Department of Physiology and Pharmacology University of BristolBristolBS8 1TDUK

The fluorescein derivative phloxine B is a potent modulator of the cystic fibrosis transmembrane conductance regulator(CFTR)Cl *** micromolar concentrations of phloxine B potentiate ATP-dependent channel gating,whereas higher concentrations occlude the channel *** determine whether fluorescein derivatives (FDs)affect the ATP affinity of CFTR and the efficacy of channel gating,we investigated the effects of FDs on the gating behaviour of CFTR using excised inside-out membrane patches from C127 cells expressing wild-type human *** employed a simple linear three-state scheme(C(?)C(?)O)to describe CFTR channel *** this scheme,C represents the long duration closed state separating channel openings,C the brief closures that interrupt channel openings,and O the open *** between the three states are described by the forward rate constantsβ,β and the backward rate constantsα andα.Given that CFTR is an agonist-activated channel gated by intracellular ATP and this scheme is reminiscent of the del castillo-Katz mechanism,we can calculate the ATP affinity(α/β)and gating efficacy of CFTR(β/α).The FDs phloxine B(1μmol/L)and eosin Y(1μmol/L) augment channel gating by prolonging mean burst duration(MBD)without changing the interburst interval(IBI), whereas dichlorofluorescein(DCF;20μmol/L)decreases the IBI without changing MBD(n=4-15).Interestingly, phloxine B(1μmol/L)and DCF(20μmol/L)decreasedα/β(enhanced affinity),whereas only phloxine B(1μmol/L) elevatedβ/α(augmented gating efficacy).To understand better how FDs affect the relationship between the ATP affinity and gating efficacy,we examined the ATP-dependence of channel gating(n>5).Increasing ATP concentrations enhanced markedly both the ATP affinity and gating efficacy of ***,phloxine B(1μmol/L) augmented both the ATP affinity and gating efficacy of CFTR,particularly at low ATP *** interpret our results to suggest that the ATP affinity and gating efficacy of CFTR are t

关键词： CFTR channel gating ATP affinity gating efficacy

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

全选清除本页清除全部题录导出标记到“检索档案”

共2页 << < 1 2 > >> 跳转到页

回到顶部

执行限定条件

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：