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Stress-induced visceral analgesia assessed non-invasively in rats is enhanced by prebiotic diet

World Journal of Gastroenterology 2012年第3期18卷 225-236页

作者： Muriel Larauche Agata Mulak Pu-Qing Yuan osamu kanauchi Yvette Taché CURE： Diges-tive Diseases Research Center and Oppenheimer Family Center for Neurobiology of Stress Department of Medicine Digestive Diseases Division at the University of California Los Angeles VA Greater Los Angeles Healthcare System Los Angeles CA 90073 United States Department of Gastroenterology and Hepatology Wroclaw Medical University Borowska 213 50-556 Wroclaw Poland University of Medical Science Shiga and Central Laboratories for Frontier Technology Kirin Holdings Co. Ltd. 1-13-5 Fukuura Kanazawa-ku Yokohama 236-0004 Japan CURE：Digestive Diseases Research Center and Oppenheimer Family Centerfor Neurobiology of Stress Department of Medicine Digestive Diseases Division at the University of California Los AngelesVA Greater Los Angeles Healthcare System

AIM: To investigate the influence of repeated water avoidance stress (rWAS) on the visceromotor response (VMR) to colorectal distension (CRD) and the modulation of the response by a prebiotic diet in rats using a novel surgery-free method of solid-state ***: Male Wistar rats fed a standard diet with or without 4% enzyme-treated rice fiber (ERF) for 5 wk were subjected to rWAS (1 h daily x 10 d) or no stress. The VMR to graded phasic CRD was assessed by intraluminal colonic pressure recording on days 0 (base-line), 1 and 10 (45 min) and 11 (24 h) after rWAS and expressed as percentage change from baseline. Cecal content of short chain fatty acids and distal colonic histology were assessed on day 11. RESULTS: WAS on day 1 reduced the VMR to CRD at 40 and 60 mmHg similarly by 28.9% ± 6.6% in both diet groups. On day 10, rWAS-induced reduction of VMR occurred only at 40 mmHg in the standard diet group (36.2% ± 17.8%) while in the ERF group VMR was lowered at 20, 40 and 60 mmHg by 64.9% ± 20.9%, 49.3% ± 11.6% and 38.9% ± 7.3% respectively. The visceral analgesia was still observed on day 11 in ERF-but not in standard diet-fed rats. By contrast the non-stressed groups (standard or ERF diet) exhibited no changes in VMR to CRD. In standard diet-fed rats, rWAS induced mild colonic histological changes that were absent in ERF-fed rats exposed to stress compared to non-stressed rats. The reduction of cecal content of isobutyrate and total butyrate, but not butyrate alone, was correlated with lower visceral pain response. Additionally, ERF diet increased rWAS-induced defecation by 26% and 75% during the first 0-15 min and last 15-60 min, respectively, compared to standard diet, and reduced rats' body weight gain by 1.3 fold independently of their stress status. CONCLUSION: These data provide the first evidence of psychological stress-related visceral analgesia in rats that was enhanced by chronic intake of ERF prebiotic.

关键词： Wistar大鼠饮食内脏评估镇痛应力诱发益生素侵入性

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Safety Evaluation of Excessive Intake of <i>Lactococcus lactis</i>Subsp. <i>lactis</i>JCM 5805: A Randomized, Double-Blind, Placebo-Controlled, Parallel-Group Trial

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Food and Nutrition Sciences 2018年第4期9卷 403-419页

作者： Yukiko Kato Masaya Kanayama Shu Yanai Hajime Nozawa osamu kanauchi Satoru Suzuki Research Laboratories for Health Science & Food Technologies Kirin Co. Ltd. Tokyo Japan Central Laboratories for Key Technologies Kirin Co. Ltd. Tokyo Japan Shinagawa Season Terrace Health Care Clinic Tokyo Japan

Background/Aims: Administration of a lactic acid bacterial strain, Lactococcus lactis subsp. lactis JCM 5805 (LC-Plasma), is reported to prevent viral infection via activation of plasmacytoid dendritic cells in mouse and human studies. As it is assumed that LC-Plasma is taken in excess when it is commercially provided as a supplement, we conducted a trial using capsules to give 250 mg LC-Plasma (5 times the effective anti-viral dose) every day for four weeks to healthy volunteers to investigate the safety of excessive intake of LC-Plasma. Trial Design: A randomized, double-blind, placebo-controlled, parallel-group trial was conducted. Methods: Forty healthy subjects were randomly assigned to the LC-Plasma group (daily intake of five capsules containing 50 mg heat-killed LC-Plasma cells per capsule) or the placebo group (daily intake of five placebo capsules with no LC-Plasma). Physical, hematological, biochemical and urinary examinations and medical interviews were used to evaluate safety. Results: No abnormal differences were observed after excessive intake of LC-Plasma capsules when compared to the intake of placebo capsules. Conclusions: There are no safety concerns associated with the excessive intake of heat-killed LC-Plasma capsules.

关键词： Lactic Acid Bacteria Lactococcus lactis Excessive Intake Safety

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Eubacterium limosum ameliorates experimental colitis and metabolite of microbe attenuates colonic inflammatory action with increase of mucosal integrity

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World Journal of Gastroenterology 2006年第7期12卷 1071-1077页

作者： osamu kanauchi Masanobu Fukuda Yoshiaki Matsumoto Shino Ishii Toyokazu Ozawa Makiko Shimizu Keiichi Mitsuyama Akira Andoh Kirin Brewery Co.Ltd. 10-1-2 Shinkawa Chuo-kuTokyo104-8288Japan College of Pharmacy Nihon University 7-7-1 Narashinodai FunabashiChiba 274-8555Japan Department of Clinical Pharmacology and Toxicology Showa Pharmaceutical University3-3165Higashi-TamagawagakuenMachidaTokyo194-8543Japan Kurume University School of Medicine 67 Asahi-machiKurumeFukuoka 830-0011Japan Shiga University of Medical Science TsukinowaSetaOtsuShiga520-2100Japan

瞄准：为了检验真细菌类高级轿车的效果，加(E。高级轿车和) 在结肠的上皮的房间线在试管内上，并且到评估 E 的效果。试验性的大肠炎上的高级轿车和。方法：E。高级轿车和被接种厌氧的联盟者和它的代谢物被获得。E 的生长 stimulatory... 详细信息

瞄准：为了检验真细菌类高级轿车的效果，加(E。高级轿车和) 在结肠的上皮的房间线在试管内上，并且到评估 E 的效果。试验性的大肠炎上的高级轿车和。方法：E。高级轿车和被接种厌氧的联盟者和它的代谢物被获得。E 的生长 stimulatory 效果。T84 房间上的高级轿车和代谢物被 IL-6 生产被 SUDH 活动，和反煽动性的效果评估。在象受体 4 一样的使用费(TLR4 ) 的 mRNA 的变化被实时 PCR 评估。大肠炎被用 2.0% 葡聚糖硫酸钠喂 BALB/C 老鼠导致。这些老鼠收到了任何一个 5% lyophilized E。高级轿车和(n = 7 ) 或控制节食(n = 7 ) 。在大肠炎正式就职，临床、组织学的分数，冒号长度，和盲肠的有机酸层次以后的七天是坚定的。结果：E。高级轿车和在 0.25， 1.0， 0.025 和 0.07 mmol/L 生产了丁酸盐，醋酸盐，丙酸盐，和 lactate，分别地在里面中等。在这集中，每酸没有生长在 T84 细胞上刺激活动；然而，当这些酸在上述层次一起被混合时，它比控制显示出显著地高的活动。除了 lactate，这些酸显著地在就 0.1 mmol/L 稀释了 IL-6 生产。在 TNF-alpha 刺激下面，另外，丁酸盐稀释了 TLR4 mRNA 的生产。有 E 的处理。高级轿车和显著地随盲肠的丁酸盐层次的增加稀释了大肠炎的临床、组织学的分数，与控制组相比。结论：E。高级轿车和能改善试验性的结肠的发炎。部分地， E 的代谢物。高级轿车和，丁酸盐，增加粘膜正直和表演经由 TLR4 的粘膜防卫系统的反煽动性的行动调整。

关键词：真菌实验研究肠炎微生物结肠炎

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