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Total Fat, Fatty Acid Composition, Tocopherol and Tocotrienol Profiles in Fermented Beans of Ten Controlled Pollinated Cocoa (Theobroma cacao L.) Hybrids from Cameroon

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American Journal of Plant Sciences 2024年第5期15卷 359-373页

作者： Martin Paul Arnaud Mbida Simon Perrez Akoa Ronelle Fabiola Mbia Manga Ndjaga Jude Martine Louise Ondobo onomo pierre effa Department of Biochemistry Faculty of Science University of Yaounde I Yaounde Cameroon Laboratory of Plant Physiology and Biochemistry Department of Biological Science Higher Teacher&#8217s Training College University of Yaounde I Yaounde Cameroon Department of Food Science and Technology Faculty of Agriculture and Veterinary Medecine University of Buea Buea Cameroon National Technology Development Committee Ministry of Scientific Research and Innovation Administrative Center Yaounde Cameroon Department of Agriculture and Agropastoral Higher Technical Teacher&#8217s Training College University of Ebolowa Ebolowa Cameroon

This study aimed to discriminate ten Cameroonian cocoa hybrids according to their total fat, fatty acid composition, tocopherol and tocotrienol profiles. Six cocoa clones from the gene banks of the Cameroon Cocoa Development Corporation were used to create hybrids. The determination of fatty acid composition was carried out by using a gas chromatography (GC) apparatus coupled by a flame ion detector (FID). Tocopherol and tocotrienol analysis was performed by upper high-performance liquid chromatography (UHPLC). Information on the impact of the genotype on the cocoa fat composition was provided. The major fatty acids (FA) in fermented samples are stearic (34.57%), palmitic (26.13%), oleic (34.13%) and linoleic (3.16%) acids. (35.05% to 35.6%). SCA12 × ICS40, SCA12 × SNK13, SNK13 × T79/501 have the least hard cocoa butters. Tocopherols analysis showed a predominance of γ-tocopherols (94.64 ± 1.51 to 292.16 ± 3.17 µg∙g−1), whereas only a small amount of β and δ-tocopherol (from 0.46 to 2.78 µg∙g−1 and 0.12 to 5.82 respectively) was observed. No γ-tocotrienol was found in fermented samples. A differentiation in terms of total fat and tocopherol content was observed amongst hybrids with the same mother-clone, suggesting an impact of pollen on these compounds.

关键词： Cocoa Hybrids Lipid Composition UHPLC GC-FID

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Primed Expression of Defense-Related Genes by Streptomyces cameroonensis-Based Bioformulation (SCaB) on Cocoa Seedlings in a Nursery Challenged with Phytophthora megakarya

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American Journal of Plant Sciences 2023年第12期14卷 1480-1497页

作者： Dzelamonyuy Aristide Téné Tayo Paul Martial Alain Bopda Waffo effa onomo pierre Ewané Cécile Annie Boudjeko Thaddée Laboratory of Phytoprotection and Valorization of Genetic Resources Biotechnology Center University of Yaounde I Yaounde Cameroon Department of Biochemistry and Molecular Biology Indiana University School of Medicine Indianapolis IN USA Department of Biochemistry Faculty of Science University of Yaounde 1 Yaounde Cameroon Laboratory of Plant Physiology Department of Biological Sciences Higher Teacher’s Training College Yaounde Cameroon

A Streptomyces cameroonensis based bioformulation (SCaB) has been developed and shown to be stable and effective in controlling the early proliferation of P. megakarya and promoting the growth of cocoa seedlings in nursery. This study was carried out to explore the molecular mechanisms associated with the interaction of SCaB, cocoa seedlings, and the pathogen during the early stages of seedling growth in the nursery. For this purpose, seedling treatment with 10% W/W SCaB under greenhouse conditions evaluated SCaB’s capacity to stimulate the defense mechanisms in cocoa. Agronomic growth parameters and the level of induction of defense-associated compounds were analyzed. Real-time (rt) PCR was used to assess the level of expression of defense genes. Here, we showed that the application of SCaB as a seedling treatment enhanced the growth of cocoa seedlings in the nursery by an average of 15.6% after 30 days of growth and led to an average reduction in disease severity of 64% when challenged with P. megakarya. The latter led to an increased synthesis of total phenolic compounds, flavonoids, chitinases, peroxidases, and β-1,3-glucanases and an induced up-regulation of TcChiB, TcGlu-1, TcPer-1, and TcMYBPA genes. This research provides a basis for the optimization of beneficial microorganisms as a viable alternative to chemical fungicides used in disease suppression.

关键词： Cocoa Seedlings Phytophthora megakarya Streptomyces cameroonensis Bioformulation Priming

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