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Molecular Plant 2019年第4期12卷 538-551页

作者： Vinay Shukla Lara Lombardi Sergio lacopino Ales Pencik ondrej novak Pierdomenico Perata Beatrice Giuntoli Francesco Licausi Unstitute of Life Sciences Scuola Superiore Sant'Anna Pisa Italy Biology Department University of Pisa Pisa Italy Laboratory of Growth Regulators Centre of the Region Hana for Biotechnological and Agricultural Research Institute of Experimental Botany CAS & Faculty of Science. Palacky University Slechtitelu 27 78371 Olomouc Czech Republic

As non-photosynthesizing organs, roots are dependent on diffusion of oxygen from the external environment and, in some in stances, from the shoot for their aerobic metabolism . Establishment of hypoxic niches in the developing tissues of plants has been postulated as a consequence of insufficient diffusion of oxygen to satisfy the demands throughout development. Here, we report that such niches are established at specific stages of lateral root primordia development in Arabidopsis thaliana grown under aerobic conditions. Using gain- and loss-of-function mutants, we show that ERF-Ⅶ transcription factors, which mediate hypoxic responses, control root architecture by acting in cells with a high level of auxin signaling. ERF-Ⅶs repress the expression of the auxin-induced genes LBD16, LBD18, and PUCHI, which are essential for lateral root development, by binding to their promoters. Our results support a model in which the establishment of hypoxic n iches in the developing lateral root primordia con tributes to the shutting down of key auxin-induced genes and regulates the production of lateral roots.

关键词： Arabidopsis auxin ERF-VII, hypoxia lateral root root development

Optimization of Root Section for Ultra-long Steam Turbine Rotor Blade

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Journal of Thermal Science 2018年第2期27卷 95-102页

作者： Jindrich Hala Martin Luxa David Simurda marek Bobcik ondrej novak Bartolomej Rudas Jaroslav Synac Institute of Thermomechanics of The Czech Academy of Sciences Prague Czech Republic Doosan Skoda Power Ltd Pilsen Czech Republic Faculty of Mechanical Engineering University of West Bohemia Pilsen Czech Republic

This study presents the comparison of aerodynamic performances of two successive designs of the root profiles for the ultra-long rotor blade equipped with a straight fir-tree dovetail. Since aerodynamic and strength requirements laid upon the root section design are contradictory, it is necessary to aerodynamically optimize the design within the limits given by the foremost strength requirements. The most limiting criterion of the static strength is the size of the blade cross-section, which is determined by the number of blades in a rotor and also by the shape and size of a blade dovetail. The aerodynamic design requires mainly the zero incidence angle at the inlet of a profile and in the ideal case ensures that the load does not exceed a limit load condition. Moreover, the typical root profile cascades are transonic with supersonic exit Mach number, therefore, the shape of a suction side and a trailing edge has to respect transonic expansion of a working gas. In this paper, the two variants of root section profile cascades are compared and the aerodynamic qualities of both variants are verified using CFD simulation and two mutually independent experimental methods of measurements(optical and pneumatic).

关键词：优化设计转子片汽轮机空气动力学蒸气工作气体侧面剖面图

Vacuole Integrity Maintained by DUF300 Proteins Is Required for Brassinosteroid Signaling Regulation

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Molecular Plant 2018年第4期11卷 553-567页

作者： Qinsong Liu Thomas Vain Corrado Viotti Siamsa M, Doyle Danuse Tarkowska ondrej novak Cyril Zipfel Folke Sitbon Stephanie Robert Daniel Hofius Department of Plant Biology Uppsala BioCenter Swedish University of Agricultural Sciences (SLU) and Linnean Center for Plant Biology PO Box 7080 750 07 Uppsala Sweden Department of Forest Genetics and Plant Physiology Umea Plant Science Centre Swedish University of Agricultural Sciences (SLU) 90183 Umea Sweden Umea Plant Science Centre Umea University 90187 Umea Sweden Institute of Biochemistry and Biology Plant Physiology University of Potsdam 14476 Potsdam Germany Laboratory of Growth Regulators Centre of the Region Hana for Biotechnological and Agricultural Research institute of Experimental Botany ASCR and Palacky University 783 71 Olomouc Czech Republic The Sainsbury Laboratory Norwich Research Park Norwich NR4 7UH UK These authors contributed equally to this article. Present addresses: Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education) College of Life Science China West Normal University Nanchong 637009 Sichuan China Institut de Biologie Moleculaire des Plantes du CNRS IBMP-CNRS UPR2357 12 rue du General Zimmer 67084 Strasbourg France

Brassinosteroid （BR） hormone signaling controls multiple processes during plant growth and development and is initiated at the plasma membrane through the receptor kinase BRASSINOSTEROID INSENSITIVE1 （BRI1） together with co-receptors such as BRI1-ASSOClATED RECEPTOR KINASE1 （BAK1）. BRI1 abun- dance is regulated by endosomal recycling and vacuolar targeting, but the role of vacuole-related proteins in BR receptor dynamics and BR responses remains elusive. Here, we show that the absence of two DUF300 domain-containing tonoplast proteins, LAZARUS1 （LAZl） and LAZl HOMOLOG1 （LAZlH1）, causes vacuole morphology defects, growth inhibition, and constitutive activation of BR signaling. Intriguingly, tonoplast accumulation of BAK1 was substantially increased and appeared causally linked to enhanced BRI1 trafficking and degradation in lazl lazlhl plants. Since unrelated vacuole mutants exhibited normal BR responses, our findings indicate that DUF300 proteins play distinct roles in the regulation of BR signaling by maintaining vacuole integrity required to balance subcellular BAK1 pools and BR receptor distribution.

关键词： brassinosteroid signaling vacuole integrity DUF300 proteins tonoplast Arabidopsis

Arabidopsis HIPP proteins regulate endoplasmic reticulum-associated degradation of CKX proteins and cytokinin responses.

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Molecular Plant 2021年第11期14卷 1918-1934页

作者： Tianqi Guo Henriette Weber Michael C.E.Niemann Lisa Theisl Georgeta Leonte ondrej novak Tomas Werner Institute of Biology/Applied Genetics Dahlem Centre of Plant Sciences(DCPS)Freie Universitat BerlinAlbrecht-Thaer-Weg 614195 BerlinGermany Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous Areas School of Life Science of Jiaying University514015 Mei ZhouChina lnstitute of Biology University of GrazSchubertstraβe 518010 GrazAustria Laboratory of Growth Regulators Centre of the Region Hana for Biotechnological and Agricultural ResearchPalacky University and Institute of Experimental Botany ASCR78371 OlomoucCzech Republic

Eukaryotic organisms are equipped with quality-control mechanisms that survey protein folding in the endoplasmic reticulum (ER) and remove non-native proteins by ER-associated degradation (ERAD). Recent research has shown that cytokinin-degrading CKX proteins are subjected to ERAD during plant development. The mechanisms of plant ERAD, including the export of substrate proteins from the ER, are not fully understood and the molecular components involved in the ERAD of CKX are unknown. We show that heavy metal-associated isoprenylated plant proteins (HIPP) interact specifically with CKX proteins synthesized in the ER and processed by ERAD. CKX-HIPP protein complexes were detected at the ER as well as in the cytosol, suggesting that the complexes involve retrotranslocated CKX protein species. Altered CKX levels in HIPP-overexpressing and higher-order hipp mutant plants suggest that the studied HIPP proteins control the ERAD of CKX. Deregulation of CKX proteins caused corresponding changes in the cytokinin signaling activity and triggered typical morphological cytokinin responses. Notably, transcriptional repression of HIPP genes by cytokinin indicates a feedback regulatory mechanism of cytokinin homeostasis and signaling responses. Moreover, the loss of HIPP genes constitutively activates the unfolded protein response and compromises the ER stress tolerance, supporting the conclusion that HIPPs represent novel functional components of plant ERAD.

关键词： Arabidopsis remove folding

A Molecular Framework for the Control of Adventitious Rooting by TIR1/AFB2-Aux/IAADependent Auxin Signaling in Arabidopsis

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Molecular Plant 2019年第11期12卷 1499-1514页

作者： Abdellah Lakehal Salma Chaabouni Emilie Cavel Rozenn Le Hir Alok Ranjan Zahra Raneshan ondrej novak Daniel IPacurar Irene Perrone Frangois Jobert Laurent Gutierrez Laszlo Bako Catherine Bellini Umea Plant Science Centre Department of Plant PhysiologyUmea University90736 UmeaSweden lnstitut Jean-Pierre Bourgin INRAAgroParisTechCNRSUniversite Paris-Saclay78000 VersaillesFrance Department of Biology Faculty of ScienceShahid Bahonar UniversityKermanIran Laboratory of Growth Regulators Faculty of SciencePalacky University and Institute of Experimental BotanyThe Czech Academy of Sciences78371 OlomoucCzech Republic Umea Plant Science Centre Department of Forest Genetics and PhysiologySwedish Agriculture University90183 UmeaSweden Centre de Ressources Regionales en Biologie Moleculaire(CRRBM) Universite de Picardie Jules Verne80039 AmiensFrance Present address:Centre de Ressources Regionales en Biologie Moleculaire(CRRBM) Universite de Picardie Jules Verne80039 AmiensFrance Present address:Institute for Sustainable Plant Protection National Research Council of ItalyTurinItaly Present address:Umea Plant Science Center Department of Forest Genetics and Plant PhysiologySLU90736 UmeaSweden

In Arabidopsis thaliana,canonical auxin-dependent gene regulation is mediated by 23 transcription factors from the AUXIN RESPONSE FACTOR(ARF)family that interact with auxin/indole acetic acid repressors(Aux/IAAs),which themselves form co-receptor complexes with one of six TRANSPORT INHIBITOR*!/AUXIN-SIGNALLING F-BOX(TIR1/AFB)proteins.Different combinations of co-receptors drive specific sensing outputs,allowing auxin to control a myriad of processes.ARF6 and ARF8 are positive regulators of adventitious root initiation upstream of jasmonate,but the exact auxin co-receptor complexes controlling the transcriptional activity of these proteins has remained unknown.Here,using loss-of-function mutants we show that three Aux/IAA genes,IAA6,IAA9,and IAA17,act additively in the control of adventitious root(AR)initiation.These three IAA proteins interact with ARF6 and/or ARF8 and likely repress their activity in AR development.We show that TIR1 and AFB2 are positive regulators of AR formation and TIR1 plays a dual role in the control of jasmonic acid(JA)biosynthesis and conjugation,as several JA biosynthesis genes are up-regulated in the tir1-1 mutant.These results lead us to propose that in the presence of auxin,TIR1 and AFB2 form specific sensing complexes with IAA6,IAA9,and/or IAA17 to modulate JA homeostasis and control AR initiation.

关键词： TIR1/AFB AuxIAA jasmonate adventitious roots Arabidopsis

FEM Study of the Strain Kinematics in the 3D Nanofibrous Structure Prepared by the Electrospinning Process

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Applied Mathematics 2013年第5期4卷 80-90页

作者： Michal Petru ondrej novak David Vejrych Petr Lepsik Institute for Nanomaterials Advanced Technologies and InovationTechnical University of LiberecLiberecCzech Republic Department of Nonwovens Technical University of LiberecLiberecCzech Republic Department of Machine Elements and Mechanism Technical University of LiberecLiberecCzech Republic

Finite element model (FEM) was used for the study and description of the arising 3D nanofiber structure strain caused by the pressure of the flowing gas. Computer simulation using an adaptive networking through implicit FEM algorithm can be utilized for a significant improvement of the study of anisotropic strain in the deformed 3D nanostructure. The created model is based on the empirical Laplace-Poisson differential equation for the flow, where gas particles are moving with certain kinetic energy. The kinetic energy depends on the speed, time and temperature and affects the resulting strain of 3D nanofiber structure. The simulation results were compared to the results obtained from the image analysis of real samples and showed that this FEM model can determine individual phases of structure strain. The comparison shows that the developed FEM model can be an important tool in the study of the strain in the arising 3D nano- fiber structure and it can provide valuable information for optimization of 3D nanofiber structure production by the electrospinning process.

关键词： FEM Strain 3D Nanofibrous Structure Electrospinning Gas Flow

Reliability-Based Optimization:Small Sample Optimization Strategy

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Journal of Computer and Communications 2014年第11期2卷 31-37页

作者： Drahomir novak ondrej Slowik Maosen Cao Institute of Structural Mechanics Faculty of Civil Engineering Brno University of TechnologyBrnoCzech Republic Department of Engineering Mechanics Hohai UniversityNanjingChina

The aim of the paper is to present a newly developed approach for reliability-based design optimization. It is based on double loop framework where the outer loop of algorithm covers the optimization part of process of reliability-based optimization and reliability constrains are calculated in inner loop. Innovation of suggested approach is in application of newly developed optimization strategy based on multilevel simulation using an advanced Latin Hypercube Sampling technique. This method is called Aimed multilevel sampling and it is designated for optimization of problems where only limited number of simulations is possible to perform due to enormous com- putational demands.

关键词： Optimization Reliability Assessment Aimed Multilevel Sampling Monte Carlo Latin Hypercube Sampling Probability of Failure Reliability-Based Design Optimization Small Sample Analysis