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Pre.aration, Characte.ization and in Vitro e.aluation of the.Antitumor Activity of the.Biologically Synthe.ize. Silve. Nanoparticle.

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Advance. in Nanoparticle. 2016年第2期5卷 149-166页

作者： omama e. elshawy e.an A. He.my Laila A. Rashe. He.lth Radiation Re.e.rch De.artme.t National Centre for Radiation Research and Technology (NCRRT) Atomic Energy Authority (AEA) Cairo Egypt Re.ional Ce.tre.of Mycology and Biote.hnology (RCMB) Al-Azhar University Cairo Egypt Me.ical Bioche.istry De.artme.t Faculty of Medicine Cairo University Cairo Egypt

This study was conce.trate. on the.biosynthe.is of silve. nanoparticle. from Pe.icillium aurantiogre.ium (IMI 89372) with a focus on its cytotoxicity in MCF-7 and MCT cance. ce.l line. as we.l as Ve.o (normal) ce.l line.that was asse.se. by crystal viole. assay afte. tre.tme.t with various conce.trations (0.44 – 145 μg/ml) for 24 h. The.ce.l morphology was e.amine. by inve.te. light microscopy. Furthe., the.radiose.sitizing e.fe.t of silve. nanoparticle. (AgNPs) on MCF-7 was also de.onstrate. by asse.sing ce.l morphology, ce.l prolife.ation of MTT assay, LDH activity and induction of apoptosis through che.king of some.apoptotic ge.e. that alte.e. during carcinoge.e.is, including caspase.3, Bax and Bcl-2. Caspase.3 activity was also e.timate.. Synthe.is of AgNPs was de.e.mine. by UV-Visible.spe.trum and it was furthe. characte.ize. by Te., FT-IR and X-Ray analysis (e.X, XRD). The.biosynthe.ize. AgNPs we.e.sphe.ical and of 12.7 nm in size.as re.orde. by dire.t e.e.tron microscopy visualization. The.biosynthe.ize. AgNPs showe. variation in cytotoxicity against MCF-7, MCT and Ve.o ce.l line. in a conce.tration de.e.dant re.ponse.with a varie. de.re. of alte.ation in ce.l morphology. The.re.ult showe. that AgNPs we.e.highly toxic towards MCF-7 with IC50 value.of 10.5 μg/ml. Tre.tme.t of MCF-7 (10.5 μg/ml) prior to irradiation improve. the.e.fe.t of irradiation dose.(6 Gy) via incre.sing alte.ation of ce.l morphology, inhibition of ce.l prolife.ation, activation of the.lactate.de.ydroge.ase.(LDH) and caspase.3 le.ding to induction of apoptosis which was furthe. confirme. through incre.sing nucle.r DNA damage.and up re.ulation of caspase.3 and Bax ge.e. and downrgulation of Bcl-2 ge.e.. In conclusion, the.pre.e.t findings cle.rly indicate. that AgNPs showe. dose.de.e.dant cytotoxicity and ve.ifie. that AgNPs acte. as a pote.t radiose.sitize. and could e.hance.gamma irradiation induce. killing of MCF-7 bre.st cance. ce.ls.

关键词： Pe.icillium aurantiogrise.m Silve. Nanoparticle. in Vitro Cytotoxicity Radio-Se.sitivity

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Ace.ic acid-and furfural-base. adaptive.e.olution of Saccharomyce. ce.e.isiae.strains for improving stre.s tole.ance.and lignoce.lulosic e.hanol production

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Chine.e.Journal of Che.ical e.gine.ring 2024年第8期72卷 26-33页

作者： omama Re.man Youduo Wu Quan Zhang Jin Guo Cuihuan Sun Huipe.g Gao Yaqing Xu Rui Xu Aye.ha Shahid Chuang Xue State.Ke. Laboratory of Fine.Che.icals Frontiers Science Centre for Smart Materials Oriented Chemical EngineeringEngineering Research Center of Application and Transformation for Synthetic BiologySchool of BioengineeringDalian University of TechnologyDalian 116024China Ningbo Institute.of Dalian Unive.sity of Te.hnology Ningbo 315016China SINOPe. Dalian Re.e.rch Institute.of Pe.role.m and Pe.roche.icals Co. Ltd.Dalian 116041China Microbial Re.e.rch Institute.of Liaoning Province Chaoyang 122000China Yunnan Provincial Rural e.e.gy e.gine.ring Ke. Laboratory Kunming 650600China US-Pak Ce.te. for Advance. Studie. in Agriculture.and Food Se.urity University of AgricultureFaisalabadPakistan

Ace.ic acid and furfural are.known as pre.ale.t inhibitors de.iving from pre.re.tme.t during lignoce.lulosic e.hanol *** ne.ative.y impact ce.l growth,glucose.uptake.and e.hanol biosynthe.is of Saccharomyce. ce.e.isiae.*** of industrial *** strains with high tole.ance.towards the.e.inhibitors is thus critical for e.ficie.t lignoce.lulosic e.hanol *** this study,the.ace.ic acid or furfural tole.ance.of diffe.e.t *** strains could be.significantly e.hance. afte. adaptive.e.olution via se.ial cultivation for 40 ge.e.ations unde. stre.s *** ace.ic acid-base. adaptive.strain SPSC01-TA9 produce. 30.5 g·L^(-1)e.hanol with a yie.d of 0.46 g·g^(-1)in the.pre.e.ce.of 9 g·L^(-1)ace.ic acid,while.the.ace.ic acid/furfural-base. adaptive.strain SPSC01-TAF94 produce. more.e.hanol of 36.2 g·L^(-1)with incre.se. yie.d up to 0.49 g·g^(-1)in the.pre.e.ce.of both 9 g·L^(-1)ace.ic acid and 4 g·L^(-1)*** improve.e.ts we.e.also obse.ve. during non-de.oxifie. corn stove. hydrolysate.culture.by SPSC01-TAF94,which achie.e. e.hanol production and yie.d of 29.1 g·L^(-1)and 0.49 g·g^(-1),re.pe.tive.y,the.growth and fe.me.tation e.ficie.cy of ace.ic acid/furfural-base. adaptive.strain in hydrolysate.was 95%highe. than those.of wildtype.strains,indicating the.ace.ic acid-and furfural-base. adaptive.e.olution strate.y could be.an e.fe.tive.approach for improving lignoce.lulosic e.hanol *** adapte. strains de.e.ope. in this study with e.hance. tole.ance.against ace.ic acid and furfural could be.pote.tially contribute.to e.onomically fe.sible.and sustainable.lignoce.lulosic biore.ine.y.

关键词： Saccharomyce. ce.e.isiae Lignoce.lulosic e.hanol production Adaptive.e.olution Ace.ic acid Furfural

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Re.shi Mushroom Atte.uate. He.atic Inflammation and Fibrosis Induce. by Irradiation e.hance. Carbon Te.rachloride.in Rat Mode.

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Journal of Bioscie.ce. and Me.icine. 2015年第10期3卷 24-38页

作者： omama e. e. Shawi Sahar S. Abd e.-Rahman Marwa Abd e. Hame.d He.lth Radiation Re.e.rch De.artme.t National Centre for Radiation Research and Technology Atomic Energy Authority Cairo Egypt De.artme.t of Pathology College of Veterinary Medicine Cairo University Drug Radiation Re.e.rch De.artme.t National Centre for Radiation Research and Technology Atomic Energy Authority Cairo Egypt

This work was unde.take. to e.tablish a ne. e.pe.ime.tal mode. of he.atic fibrosis by gamma irradiation and CCl4 and to study the.he.atoprote.tive.e.fe.t of Re.shi Mushroom (RM) against he.atic fibrosis induce. in that mode.. Our re.ults re.e.le. that oral co-administration of 110 mg/kg RM by gavage.to fibrotic rats offe.e. an obvious he.atic prote.tion as assure. by the.significant de.re.e.t in ALT and AST, HP conte.t, MDA and NO le.e.s with e.e.ation of the.antioxidant e.zyme. activitie.. The.le.e.s of TGF-β, TNF-α, HO-1 and type.1 collage. and the.r m-RNA e.pre.sion we.e.marke.ly de.line. as compare. with those.of fibrotic rats. Microscopical e.amination re.e.le. that the.e.posure.of rats to radiation aggravate. the.e.fe.t of CCl4 causing e.te.sive.collage. de.osition and marke. pse.dolobulation of the.he.atic pare.chyma indicative.of bridging fibrosis. While. oral co-administration of RM obviously improve. the.state.of ste.tosis and appare.tly suppre.se. he.atic fibroge.e.is.

关键词： Carbon Te.rachloride Gamma Irradiation He.atic Fibrosis Re.shi Mushroom Rats

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