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Super-resolution visible photoactivated atomic force microscopy

Light(Science & Applications) 2017年第1期6卷 441-449页

作者： Seunghyun Lee Owoong Kwon Mansik Jeon Jaejung Song Seungjun Shin HyeMi Kim Minguk Jo Taiuk rim Junsang Doh Sungjee Kim Junwoo Son Yunseok Kim Chulhong Kim Future IT Innovation Laboratory Department of Creative IT EngineeringPohang University of Science and Technology(POSTECH)PohangRepublic of Korea School of Advanced Materials Science and Engineering Sungkyunkwan University(SKKU)SuwonRepublic of Korea School of Electronics Engineering Kyungpook National University(KNU)DaeguRepublic of Korea School of Interdisciplinary Bioscience and Bioengineering Pohang University of Science and Technology(POSTECH)PohangRepublic of Korea Division of Integrative Biosciences and Biotechnology Pohang University of Science and Technology(POSTECH)PohangRepublic of Korea Department of Materials Science and Engineering Pohang University of Science and Technology(POSTECH)PohangRepublic of Korea Department of Mechanical Engineering Pohang University of Science and Technology(POSTECH)PohangRepublic of Korea Department of Chemistry Pohang University of Science and Technology(POSTECH)PohangRepublic of Korea

Imaging the intrinsic optical absorption properties of nanomaterials with optical microscopy(om)is hindered by the optical diffraction limit and intrinsically poor ***,expensive and destructive electron microscopy(EM)has been commonly used to examine the morphologies of ***,while nanoscale fluorescence om has become crucial for investigating the morphologies and functions of intracellular specimens,this modality is not suitable for imaging optical absorption and requires the use of possibly undesirable exogenous fluorescent molecules for biological *** we demonstrate super-resolution visible photoactivated atomic force microscopy(pAFM),which can sense intrinsic optical absorption with~8 nm ***,the resolution can be improved down to~8 *** system can detect not only the first harmonic response,but also the higher harmonic response using the nonlinear *** thermoelastic effects induced by pulsed laser irradiation allow us to obtain visible pAFM images of single gold nanospheres,various nanowires,and biological cells,all with nanoscale *** expensive EM,the visible pAFM system can be simply implemented by adding an optical excitation sub-system to a commercial atomic force microscope.

关键词： Arabidopsis imaging gold nanoparticle imaging melanoma cell imaging nanowire imaging super-resolution optical microscopy

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