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国外医学（输血及血液学分册） 2004年第6期27卷 554-556页

作者： olsson ml 秦斐浙江省血液中心

免疫血液学领域的最重要的目的是保证给病人提供成功的血液输注。对于红细胞输注而言，这主要包括血红蛋白水平的预期提高和避免发生速发或迟发输血副作用。上个世纪，大量重要的发现使该领域有了显著的发展，血液供者和受者之间的血型... 详细信息

免疫血液学领域的最重要的目的是保证给病人提供成功的血液输注。对于红细胞输注而言，这主要包括血红蛋白水平的预期提高和避免发生速发或迟发输血副作用。上个世纪，大量重要的发现使该领域有了显著的发展，血液供者和受者之间的血型相容性有了显著提高。很明显其中突出的部分包括ABO血型分型、交叉配血、抗人球蛋白试验及后来与输血前检测相关的ABO之外多种其它的血型抗原和抗体的阐明。在20世纪后期，血清学方法学上发生了一些有意义的进展，如低离子强度溶液、蛋白酶处理、自动化装置、增强剂、单克隆抗体和最新的检测系统如凝胶和固相方法。另外二十世纪八十年代末和九十年代兴起的分子生物学革命开始影响免疫血液学，从而给这个经典的学科开拓了崭新的时代。

关键词：免疫血液学血液输注红细胞输注血型相容性交叉配血

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O等位基因引起ABO基因型与表现型定型差异

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国际输血及血液学杂志 2009年第3期32卷 282-284页

作者： Yazer MH Hosseini-Maaf B olsson ml 陈雪(节译) 朱发明(审校) 不详成都市血液中心 610041

保证输血时血清学方面的安全，首要的是对受血者与献血者ABO血型定型，血清学检查通常分两个步骤。正定型通常使用鼠源单克隆抗体检测红细胞表面是否存在A或B抗原。互补的实验即反定型，利用当红细胞上缺乏A或B抗原时，人群可天然产生... 详细信息

保证输血时血清学方面的安全，首要的是对受血者与献血者ABO血型定型，血清学检查通常分两个步骤。正定型通常使用鼠源单克隆抗体检测红细胞表面是否存在A或B抗原。互补的实验即反定型，利用当红细胞上缺乏A或B抗原时，人群可天然产生相对应的抗体的原理，检测血清中是否存在抗-A或者抗B抗体。确定了受血者红细胞表面的ABO抗原以及血浆中的抗体，便能确定血型，为其提供相合的血液。

关键词： ABO抗原血型定型等位基因单克隆抗体检测表现型基因型血清学检查红细胞表面

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Bioreactance and Apelin in the Management of Severe Hyponatremia

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Open Journal of Emergency Medicine 2021年第1期9卷 1-10页

作者： Karin olsson Magnus Löndahl Olle Melander Per Katzman Department of Clinical Sciences Lund University Lund Sweden Department of Endocrinology Skane University Hospital Lund Sweden Department of Emergency and Internal Medicine Skane University Hospital Malm&#246 Sweden

Hyponatremia is a severe electrolyte disturbance associated with substantial morbidity and mortality. It often poses a diagnostic and therapeutic challenge. Accurate assessment of patient fluid-volume status is central to effective management. This pilot study aimed to evaluate the usefulness of the Cheetah NICOM bioreactance system and apelin in early differentiation between hypo- and euvolemia in patients with severe hyponatremia. Methods: Patients > 50 years of age with a serum sodium ≤ 125 mmol/L were eligible for inclusion after written informed consent. Blood- and urine analyses of cardiovascular load (NT-proBNP), osmotic stress (copeptin, apelin, osmolality, sodium), mineralocorticoid status (aldosterone, renin) and sympathetic activity (methoxycathecholamines) were analysed at baseline and after isotonic sodium chloride infusion. Bedside bioreactance examination was used to visualise parameters, including stroke volume before and after passive leg raise test. Classification of volume status was made retrospectively blinded for biomarker and bioreactance results. Results: 8 patients (4 hypovolemic and 4 euvolemic), 79 years old, median plasma sodium 120 mmol/L were included. At the Emergency Department volume status was misclassified in all hypo- and in 2 of 4 euvolemic patients. Apelin was significantly higher in hypovolemic patients ((299 vs. 175 ng/ml), p = 0.021). All hypovolemic, but none of the euvolemic, patients had a level above 250 ng/ml. Copeptin did not differ between groups. All patients in the hypovolemic group increased their stroke volume after passive leg raise. Conclusions: Apelin seems to be a promising future biomarker in the early management of severe hyponatremia. Bioreactance measurements may offer a supplement to bedside evaluation of volume status.

关键词： Hyponatremia Apelin Vasopressin

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Effect of phosphate and potassium solubilizing bacteria on tobacco plant growth

Effect of phosphate and potassium solubilizing bacteria on t...

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中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Sabai Thein Zhang Chengkang Wang Shumin Liu Hong Stefan olsson Wang Zonghua College of Resource and Environmental Science Fujian Agriculture and Forestry University Department of Botany University of Yangon College of Plant Protection Fujian Agriculture and Forestry University

In this research,investigation of(6) phosphate and potassium solubilizing bacteria from the total of(37) strains uiat isolated from(4) different soil samples of *** solubilizing activities of isolated bacteria were analyzed qualitative and qualitative by agar and broth media as PVK,NBRIP for phosphate solubilizing and AKD for *** the(6) solubilizing strains,S was represented the maximum solubilizing abilities in broth media of PVK,NBRIP(48.71μg/ml,59.66μg/ml) and AKD media(20.49μg/ml).S16 showed as the second highest activities in PVK(36.42μg/ml),NBRIP(38.10μg/ml) and AKD(24.00μg/ml).The sugar fermentation,protease enzyme and siderophore production tests were supported the P,K solubilizing activities of selected *** the basic of 16S rRNA gene sequencing,all(6) strains were closely related widi each ***(4) strains such as S,S,S and S were represented as Bacillus thuringiensis and(2) strains S and S were observed as Lysinibacillus *** examination with gyrB gene sequencing processes,S,S,Sand S were also shown as Bacillus thuringiensis serovar poloniensis,Bacillus thuringiensis serovar kenyae,Bacillus thuringiensis serovar nigeriensis and Bacillus thuringiensis serovar ***,Swas associated with Lysinibacillus sphaericus and S was Lysinibacillus *** to the analyzed of solubilizing acitivites and phylogenetic analysis,S31(Lysinibacillus sphaericus) indicated that the highest P,K solubilizing bacteria among the other ***,the isolated bacteria S16,S31,(S16 + S31) and Sbm(positive control) were used as biofertilizer effect on tobacco plant growth in green *** different ratio of chemical fertilizer and bacteria were utilized to determine the effect of PSB,KSB on tobacco plant *** this experiment,(17) treatments were designed with(3) *** this experiment,plant parameters(plant height,total leaves area and plant biomass) were *** plant samples an

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