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Fractionation and fixation of rare earth elements in soils:Effect of spiking with lanthanum,cerium,and neodymium chlorides

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Journal of Rare Earths 2022年第1期40卷 143-152页

作者： olga B.Rogova Petr S.Fedotov Rustam kh.Dzhenloda Vasily K.Karandashev V.V.Dokuchaev Soil Science Institute Russian Academy of Sciences7 Building 2 Pyzhevskiy Side StreetMoscow119017Russia V.I.Vernadsky Institute of Geochemistry and Analytical Chemistry Russian Academy of Sciences19 Kosygin StreetMoscow119991 Russia National University of Science and Technology'MISiS' 4 Leninsky ProspectMoscow119049Russia The Institute of Microelectronics Technology and High-Purity Materials Russian Academy of Sciences6 Institution StreetChernogolovka142432Russia

Industrial and agricultural activities lead to the release of rare earth elements(REEs)in wastewater and aquatic ecosystems,and their accumulation in ***,the behavior of REEs in soils remains somewhat *** the present work the fractionation and fixation of REEs in soddy-podzolic and chernozem soils spiked with La,Ce,and Nd chlorides were studied using dynamic(continuous flow)extraction,which allows natural conditions to be mimicked and artefacts to be *** eluents applied are aimed to dissolve exchangeable,specifically sorbed,bound to Mn oxides,bound to metal-organic complexes,and bound to amorphous and poorly ordered Fe/Al oxides fractions extractable by 0.05 mol/L Ca(NO_(3))2,0.43 mol/L CH_(3)COOH,0.1 mol/L NH_(2)OH·HCl,0.1 mol/L K_(4)P_(2)O_(7) at pH 11,and 0.1 mol/L(NH4)_(2)C_(2)O_(4) at pH 3.2,*** is found that the fixations of added La,Ce,and Nd in the form of metal-organic complexes is predominant for both types of soils:35%-38%in soddy-podzolic soil and 50%-79%in *** fixation of added elements in the first three fractions(exchangeable,specifically sorbed,and bound to Mn oxides)is significant for soddy-podzolic soil(5%-25%).For chernozem,the relative contents of added Ce and Nd in these fractions are nearly *** the content of exchangeable La is notable,about 5%.Adding any of three elements(La,Ce,or Nd)at the level of100 mg/kg to an initial sample results in changing the fractionation and bioaccessibility of other REEs present in *** contents increase in the first three fractions and decrease in fifth(oxalate extractable)fraction for both soddy-podzolic soil and *** main difference is the behavior of REEs in pyrophosphate extractable *** soddy-podzolic soil,adding La,Ce,or Nd results in decreasing the contents of other REEs associated with organic *** chernozem,on the contrary,the contents of REEs in the form of metal-organic complexes slightly *** processes

关键词： Rare earth elements Soddy-podzolic soil Chernozem Fractionation Competitive binding

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Predicting anatomical results of surgical treatment of idiopathic macular hole

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International Journal of Ophthalmology(English edition) 2016年第2期9卷 253-257页

作者： Alexander A.Shpak Dmitry O.Shkvorchenko Ilias kh.Sharafetdinov olga A.Yukhanova The S. Fyodorov Eye Microsurgery Federal State Institution Moscow 127486 Russia

AIM： To determine the parameters most informative in predicting the anatomical results of surgical treatment of idiopathic full-thickness macular hole （IMH）. METHODS： One hundred and sixty-two consecutive patients （170 eyes） after primary operation for IMH were enrolled. Outcomes were classified as anatomical success when both IMH closure and restoration of the outer retinal structure were achieved. ＂Prospective＂ group included 108 patients （115 eyes） followed with optical coherence tomography （OCT） and microperimetry for ly after surgery. Potential prognostic criteria, except microperimetry data, were tested in ＂retrospective＂ group （54 patients, 55 eyes）. Prognostic value of each parameter was determined using receiver operating characteristic （ROC） analysis. Combined predictive power of the best prognostic parameters was tested with the use of linear discriminant analysis. RESULTS： IMH closure was achieved in 106 eyes （92%） in the prospective group and 49 eyes （89%） in the retrospective group. Despite anatomical closure, the outer retinal structure was not restored in two eyes in the first group and in one eye in the second group. Preoperative central subfield retinal thickness demonstrated the best discriminatory capability between eyes with anatomical success and failure： area under the ROC-curve （AUC） 0.938 （95% Ch 0.881-0.995）, sensitivity 64% at fixed specificity 95% （cut-off value 300um） in the prospective group; sensitivity 57% and specificity 90% in the retrospective group. Other continuous parameters except tractional hole index （AUC： 0.796, 95% Ch 0.591- 1.000） had significantly lower AUCs （P〈0.05）. The best combination of the parameters, established by discriminant analysis in the prospective group, could not confirm its predictive value in the retrospective group. CONCLUSION： Preoperative central subfield retinal thickness is a strong and probably the best predictor of anatomical results of IMH surgical treatment.

关键词： idiopathic full-thickness macular hole optical coherence tomography anatomical surgery outcome prognosis central subfield retinal thickness receiveroperating characteristic analysis

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Molecular characteristics of rifampin and isoniazid resistant Mycobacterium tuberculosis strains from Beijing,China

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Chinese Medical Journal 2007年第9期120卷 814-819页

作者： JIAO Wei-wei Mokrousov Igor SUN Gui-zhi LI Mo LIU Jia-wen Narvskaya olga SHEN A-dong Beijing Pediatric Institute Beijing Children＇s Hospital Affiliated to Capital Medical University Beijing 100045 China Laboratory of Molecular Microbiology St. Petersburg Pasteur Institute St. Petersburg 197101 Russia Clinical Laboratory Beijing Chest Hospital Beijing 100095 China

Background China is one of the high burden countries of Mycobacterium tuberculosis （TB） infection globally, with high incidence and mortality. We studied the molecular characteristics of rifampin （rif） and isoniazid （INH） resistant Mycobacterium tuberculosis strains from Beijing, China, in order to find out the genetic marker for rapid detection of specific drug resistance.Methods Forty pansusceptible and 81 resistant strains of Mycobacterium tuberculosis isolated from Beijing, China during 2002-2005 were analyzed. The modified rifampin oligonucleotide （rifO） assay based on reverse line blot hybridization was used to detect mutations in the 81 bp hot-spot region of rpoB gene, which is associated with rif resistance. The INH resistance associated genes, regulatory region mab-inhA （-15C/T） and structural gene katG S315T were detected by reverse line blot hybridization and PCR-restriction fragment length polymorphism （RFLP） method respectively. All the strains were typed by spoligotying and the Beijing genotype was further subdivided by NTF locus analysis. The distribution of drug resistance associated mutations in the above genes was compared in these groups. Results Sixty-five （91.5%） of 71 rif resistant and 52 （92.9%） of 56 multidrug-resistant （MDR, i.e. resistant to at least rif and INH） strains were found to harbor mutations in the rpoB hot-spot region. No mutation was detected in rif sensitive strains. The specificity and sensitivity of the modified rifO assay were 100% and 91.5%, respectively, katG315 AGC〉ACC and inhA-15C〉T mutations were found in 40 （60.6%） and 10 （15.2%） of 66 INH resistant strains, respectively; 7.6% of INH-resistant strains had mutations in both of these genes. Therefore, a combined use of both katG315 and inhA-15 identified 68.2% of INH-resistant strains. The Beijing genotype accounted for 91.7% of total strains and was further subdivided into ＂modern＂（76.6%） and ＂ancestral＂（23.4%） group. There is no significant difference between ＂an

关键词： Mycobacterium tuberculosis drug resistance rifampin isoniazid

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Experience on the first national anti-TB drug resistance survey(DRS)in Timor-Leste

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Global Health Research and Policy 2022年第1期7卷 415-423页

作者： Constantino Lopes Debashish Kundu Ismael Da Costa Barreto Bernardino da Cruz SSiva Kumar Sureshbabu Ramalingam Anna SDean Vineet Bhatia Prabhu Seenivasan CPadmapriyadarsini olga Tosas Auguet National TB Programme Ministry of HealthDiliTimor-Leste World Health Organization Country Office for Timor-Leste DiliTimor-Leste National Health Laboratory Ministry of HealthDiliTimor-Leste National HIV Programme Ministry of HealthDiliTimor-Leste National Institute for Research in Tuber-culosis ChennaiIndia National TB Reference Laboratory DiliTimor-Leste Global Tuberculosis Programme World Health OrganizationGenevaSwitzerland World Health Organization South East Asia Regional Office New DelhiIndia

Background:A national drug resistance survey(DRS)was implemented for the first time in Timor-Leste(TL)in *** primary objective of the survey was to assess the prevalence of drug resistance among new and previously treated pulmonary TB patients in the ***:This nation-wide cross-sectional survey was conducted in 2019 targeting all new and previously treated sputum smear-positive pulmonary TB *** samples were submitted to the National TB Reference Laboratory for confirmation of TB and to determine resistance to rifampicin by Xpert MTB/*** was performed on solid media,and culture isolates of confirmed TB cases were shipped to the WHO Supranational TB Reference Laboratory in Chennai,India for whole genome sequencing(WGS).Survey summary statistics,data cross-tabulations and analysis of potential risk factors of rifampicin-resistant TB(RR-TB)were conducted using R statistical software(version 3.5.2).Results:A total of 953 sputum-smear positive patients were enrolled,of which 917 were confirmed as positive for TB by either Xpert MTB/rif or *** electronic web-based system was used for entry and storage of the *** resistance was detected among 0.6%(95%CI 0.2-1.3)of new cases and 2.7%(95%CI 0.5-8.2)of previously treated *** was conducted for validation purposes on 65 randomly selected isolates(29%of RR-TB(2/7)and 7%of RS-TB(63/910)by Xpert MTB/rif or pDST).The original test results agreed with the WGS validation results for 62/64 isolates(97%).Conclusion:The prevalence of RR-TB in Timor-Leste is relatively low compared to the estimated proportions of RR-TB in the WHO South-East Asia Region(2.5%[95%CI 1.9-3.3]among new cases and 14%[95%CI 7.7-21]among previously treated cases).The rapid sputum collection and transportation mechanism implemented in the survey demonstrates its feasibility in low resource settings and should be replicated for routinely transporting TB specimens from microscopy labs to GeneXpert ***

关键词： Anti-TB Drug Resistance Survey rifampicin resistance Whole genome sequencing Timor-Leste

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rifO杂交和限制性片段长度多态性检测耐利福平和异烟肼的结核分枝杆菌

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中华检验医学杂志 2007年第7期30卷 733-736页

作者：焦伟伟 Igor Mokmusov 孙桂芝李墨刘佳文 olga Narvskaya 申阿东首都医科大学附属北京儿童医院儿科研究所 100045 俄罗斯圣彼得堡巴斯德研究所圣彼得堡197101 北京胸科医院检验科

目的评价利福平寡核苷酸探针杂交技术(rifO 杂交)和 PCR-限制性片段长度多态性(PCR-RFLP)在结核分枝杆菌(MTB)耐利福平(rif)和异烟肼(INH)快速检测中的应用价值。方法选取121株北京地区 MTB 菌株,分别采用 rifO 杂交技术和 PCR-RFLP 检... 详细信息

目的评价利福平寡核苷酸探针杂交技术(rifO 杂交)和 PCR-限制性片段长度多态性(PCR-RFLP)在结核分枝杆菌(MTB)耐利福平(rif)和异烟肼(INH)快速检测中的应用价值。方法选取121株北京地区 MTB 菌株,分别采用 rifO 杂交技术和 PCR-RFLP 检测 rif 耐药相关基因 rpoB 核心区和 INH 耐药相关基因 katG315位点突变,并对所有菌株的 rpoB 基因核心区进行测序验证。结果 rifO 杂交检测发现,91.5%(65/71)的 rif 耐药株和92.9%(52/56)的耐多药菌株(至少对 rif 和 INH 耐药)存在 rpoB 基因核心区突变,而 rif 敏感株中未发现突变;rifO 杂交与测序结果完全一致,测序结果中有突变的位点在 rifO 杂交中均有相应的野生型杂交信号缺失;PCR-RFLP 结果显示,INH 耐药株中 katG315突变率为60.6%(40/66)。结论 rpoB 基因核心区可作为 rif 耐药检测的分子标志及耐多药的筛选指标;rifO 杂交技术是检测 MTB 耐 rif 的快速、准确的实验方法,具有推广及潜在的临床应用价值;PCR-RFLP 可检测出大部分 INH 耐药株,可作为临床 INH 耐药性检测的辅助手段。

关键词：分枝杆菌结核抗药性利福平异烟肼 rifO杂交

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结核分枝杆茵耐药性快速检测及基因分型研究

结核分枝杆茵耐药性快速检测及基因分型研究

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中华医学会第十四次全国儿科学术会议

作者：申阿东焦伟伟 Igor Mokrousov 李墨孙桂芝刘佳文 olga Narvskaya 首都医科大学附属北京儿童医院

目的:研究北京地区结核分枝杆菌利福平(rif)和异烟肼(INH)耐药特点,寻找理想的基因位点作为耐药检测分子标志;了解北京地区结核分枝杆菌的基因型分布特点;评价改进的利福平寡核昔酸探针杂交技术(rifO杂交)在结核分枝杆菌耐药性快速检测...

目的:研究北京地区结核分枝杆菌利福平(rif)和异烟肼(INH)耐药特点,寻找理想的基因位点作为耐药检测分子标志;了解北京地区结核分枝杆菌的基因型分布特点;评价改进的利福平寡核昔酸探针杂交技术(rifO杂交)在结核分枝杆菌耐药性快速检测中的应用价值。方法:共选用121株北京地区结核分枝杆菌,采用改进的rifO杂交技术对利福平耐药相关基因rpoB核心区及对异烟肼耐药菌株的inhA突变情况进行分析;采用聚合酶链反应．限制性片段长度多态性(PCR-RFLP)检测异烟肼耐

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结核分枝杆菌利福平和异烟肼耐药特点分析

结核分枝杆菌利福平和异烟肼耐药特点分析

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中华医学会结核病学分会2006年学术会议

作者：申阿东焦伟伟李墨 Igor Mokrousov olga Narvskaya 孙桂芝刘佳文首都医科大学附属北京儿童医院俄罗斯圣彼得堡巴斯德研究所北京胸科医院检验科

目的研究北京地区结核分枝杆菌利福平(rif)和异烟肼(INH)耐药特点,寻找理想的基因位点作为耐药检测分子标志:了解北京地区结核分枝杆菌的基因型分布特点;评价改进的利福平寡核苷酸探针杂交技术(rifO杂交)在结核分枝杆菌耐药性快速检测...

目的研究北京地区结核分枝杆菌利福平(rif)和异烟肼(INH)耐药特点,寻找理想的基因位点作为耐药检测分子标志:了解北京地区结核分枝杆菌的基因型分布特点;评价改进的利福平寡核苷酸探针杂交技术(rifO杂交)在结核分枝杆菌耐药性快速检测中的应用价值。

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