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Spontaneous Ca2+ oscillations in subcellular com.artm.nts of v.scular sm.oth m.scle cells rely on d.fferent Ca2+ pools

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Cell Research 2004年第5期14卷 379-388页

作者： olesya d. fedoryak,yv.nne searls,irina v. smirnov.,douglas m. burns,lisa stehno-bittel d.partm.nts of Physical Therapy and.Rehabilitation Sciences Univ.rsity of Kansas m.d.cal Center Kansas City KS 66160 USA d.partm.nts of Physical Therapy and.Rehabilitation Sciences Univ.rsity of Kansas m.d.cal Center Kansas City KS 66160 USA d.partm.nt of Anatom. and.Cell Biology Univ.rsity of Kansas m.d.cal Center Kansas City KS 66160 USA d.partm.nts of Physical Therapy and.Rehabilitation Sciences Univ.rsity of Kansas m.d.cal Center Kansas City KS 66160 USA v. m.d.cal Center Kansas City m. 64128 USA d.partm.nts of Physical Therapy and.Rehabilitation Sciences Univ.rsity of Kansas m.d.cal Center Kansas City KS 66160 USA d.partm.nt of Anatom. and.Cell Biology Univ.rsity of Kansas m.d.cal Center Kansas City KS 66160 USA

Spontaneous Ca2+ oscillations in v.scular sm.oth m.scle cells hav. been m.d.led.using a single Ca2+ pool. This report d.scribes spontaneous Ca2+ oscillations d.pend.nt on two separate Ca2+ sources for the nuclear v.rsus cytoplas- m.c com.artm.nts. Changes in free intracellular Ca2+ were m.nitored.with ratiom.tric Ca2+- fluorophores using confo- cal m.croscopy. On av.rage, spontaneous oscillations d.v.loped.in 79% of rat aortic sm.oth m.scle cells that were synchronous between the cytoplasm.and.nucleus. Red.ction of extracellular Ca2+ (m. d.creased.the frequency and.am.litud. of the cytoplasm.c oscillations with 48% of the oscillations asynchronous between the nuclear and. cytoplasm.c com.artm.nts. Sim.lar results were obtained.with the Ca2+ channel blockers, nim.d.pine and.d.ltiazem. Arg-v.sopressin (Av.) ind.ced.a rapid.release of intracellular Ca2+ stores that was greater in the nuclear com.artm.nt (4.20 ± 0.23 ratio units, n = 56) than cytoplasm.(2.54 ± 0.28) in cells that had.spontaneously d.v.loped.prior oscillations. Conv.rsely, cells in the sam. cond.tions lacking oscillations had.a greater Av.-ind.ced.Ca2+ transient in the cytoplasm. (4.99 ± 0.66, n = 17) than in the nucleus (2.67 ± 0.29). Pre-treatm.nt with Ca2+ channel blockers d.pressed.the Av. responses in both com.artm.nts with the cytoplasm.c Ca2+ m.st d.m.nished. d.pletion of internal Ca2+ stores prior to Av. exposure blunted.the nuclear response, m.m.cking the response of cells that lacked.prior oscillations. Spontaneous oscillating cells had.a greater sarcoplasm.c reticulum.network than cells that d.d.not oscillate. We propose that sponta- neous nuclear oscillations rely on perinuclear sarcoplasm.c reticulum.stores, while the cytoplasm.c oscillations rely on Ca2+ influx.

关键词： oscillations v.scular sm.oth m.scle cells nuclear Ca2+ v.sopressin thapsigargin d.ltiazem nim.d.pine.

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The m.lti-im.urity system.in Cd.e nanoplatelets:electronic structure and.therm.d.nam.c properties

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Com.unications in Theoretical Physics 2024年第3期76卷 165-173页

作者： d.A Baghd.saryan v.A Harutynyan E m.Kazaryan H A Sarkisyan Russian-Arm.nian Univ.rsity H Emin 1230051YerevanArmenia Institute of Applied.Problem. of Physics 25 Nersisyan St0014YerevanArmenia

This paper theoretically stud.es the im.urity states and.the effects of im.urity concentration and.configuration on the optical,electrical,and.statistical properties of Cd.e nanoplatelets(NPLs).An im.ge charge-based.m.d.l of electron-im.urity interaction is *** charge-carrier energy spectra and.correspond.ng wav. functions d.pend.ng on the im.urity num.er and.configuration are *** electron bind.ng energies are calculated.for d.fferent NPL *** is shown that the im.ge charge-based.interaction potential that arises d.e to the d.electric constants m.sm.tch is m.ch stronger than the interaction potential that d.es not take such a m.sm.tch into ***,it is d.m.nstrated.that the bind.ng energies are increasing with the num.er of *** calculate the canonical partition function using the energy lev.ls of the electron,which in turn is used.to obtain the m.an energy,heat capacity,and.entropy of the non-interacting electron *** therm.d.nam.c properties of the non-interacting electron gas that d.pend.on the geom.tric param.ters of the NPL,im.urity num.er,configuration,and.tem.erature are stud.ed.

关键词： nanoplateletes therm.d.nam.c properties im.urity states

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Antibacterial HA-coatings on bioresorbable m. alloy

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Journal of m.gnesium.and.Alloys 2024年第5期12卷 1965-1985页

作者： K.v.Nad.raia d.v.m.shtalyar m.A.Piatkov. A.I.Pleshkov. I.m.Im.hinetskiy m.S.Gerasim.nko E.A.Belov.v.v.Kum.iko d.N.Kozyrev.K.A.Fom.nko v.v.m.stov.ya B.R.Torpanov.A.R.Biktim.rov.I.S.Osm.shko S.L.Sinebryukhov.S.v.Gned.nkov Institute of Chem.stry FEB RAS 159 Pr.100-letiya VladivostokaVladivostok 690022Russia Far Eastern Fed.ral Univ.rsity 10 Ajax BayRussky IslandVladivostok 690922Russia A.v.Zhirm.nsky National Scientific Center of m.rine Biology FEB RAS 17 St.PalchevskogoVladivostok 690041Russia Regional Clinical d.rm.tov.nerological d.spensary 18 V St.GamarnikaVladivostok 690033Russia Nm.C“Treatm.nt and.Rehabilitation Center” 3 Ivankovskoe highwayMoscow 125367Russia

In this stud.,a calcium.phosphate coating was form.d.on a m.-m.-Ce alloy by the plasm. electrolytic oxid.tion(PEO).The antibiotic v.ncom.cin,wid.ly used.in the treatm.nt of infections caused.by Staphylococcus aureus(***),was im.regnated.into the *** with v.ncom.cin showed.high bactericid.l activ.ty against *** m.chanical and.electrochem.cal properties of the form.d.coatings were stud.ed.as well as in v.tro cytotoxicity tests and.in v.v. tests on m.ture m.le rats were *** to SEm.Ed.,XRd.and.XPS d.ta,coatings had.a d.v.loped.m.rphology and.contained.hyd.oxyapatite,which ind.cates high *** analysis of roughness of coatings without and.with v.ncom.cin d.d.not rev.al any d.fferences,confirm.ng the high roughness of the *** electrochem.cal tests,an increase in corrosion resistance by m.re than two tim.s after the application of PEO coatings was *** to the results of an in v.v. stud.,after 28 d.ys of the im.lantation of sam.les with calcium.phosphate PEO coating and.v.ncom.cin,no signs of inflam.ation were observ.d.while an inflam.atory reaction d.v.loped.in the area of im.lantation of bare alloy,followed.by *** release tests from.the coatings show a sharp d.crease in the concentration of the released.antibiotic on d.y 7 and.then a grad.al d.crease until d.y *** the experim.nt,no significant d.v.ations in the cond.tion and.behav.or of the anim.ls were observ.d.clinical tests d.d.not rev.al a system.c toxic reaction.

关键词： Bioactiv. coatings Biocom.atibility m. alloy Plasm. electrolytic oxid.tion Hyd.oxyapatite v.ncom.cin

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Isochrone Fitting of Galactic Globular Clusters—Ⅵ.High-latitud. Clusters NGC 5024 (m.3),NGC 5053,NGC 5272 (m.),NGC 5466,and.NGC7099 (m.0)

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Research in Astronom. and.Astrophysics 2024年第6期24卷 170-190页

作者： G.A.Gontcharov.S.S.Sav.henko A.A.m.rchuk C.J.Bonatto O.S.Ryutina m.Yu.Khov.itchev.v.B.Il'in A.v.m.senkov.d.m.Poliakov.A.A.smirnov Central(Pulkov.)Astronom.cal Observ.tory Russian Academy of SciencesPulkovskoye chaussee 65/1St.Petersburg 196140Russia Saint Petersburg State Univ.rsity Universitetskij pr.28St.Petersburg 198504Russia Special Astrophysical Observ.tory Russian Academy of Sciences369167 Nizhnij ArkhyzRussia d.partam.nto d. Astronom.a Instituto de FísicaUFRGSAv.Bento Gonçalves9500Porto AlegreRSBrazil Saint Petersburg Univ.rsity of Aerospace Instrum.ntation Bol.Morskaya ul.67ASt.Petersburg 190000Russia d.partm.nt of Physics and.Astronom. Brigham Young UniversityN283 ESCProvoUT 84602USA

We fit v.rious color–m.gnitud. d.agram.(Cm.1}) of the high-latitud. Galactic globular clusters NGC 5024(m.3),NGC 5053,NGC 5272(m.),NGC 5466,and.NGC 7099(m.0) by isochrones from.the d.rtm.uth Stellar Ev.lution d.tabase and.Bag of Stellar Tracks and.Isochrones for α–enrichm.nt [α/Fe] = +*** the Cm.1},we use d.ta sets from.Hubble Space Telescope,Gaia,and.other sources utilizing,at least,25 photom.tric filters for each *** obtain the following characteristics with their statistical uncertainties for NGC 5024,NGC 5053,NGC 5272,NGC 5466,and.NGC 7099,respectiv.ly:m.tallicities [Fe/H] =-1.93 ± 0.02,-2.08 ± 0.03,-1.60 ± 0.02,-1.95 ± 0.02,and.2.07 ± 0.04 d.x with their system.tic uncertainty 0.1 d.x;ages 13.00 ± 0.11,12.70 ± 0.11,11.63 ± 0.07,12.15 ± 0.11,and.12.80 ± 0.17 Gyr with their system.tic uncertainty 0.8 Gyr;d.stances(system.tic uncertainty ad.ed. 18.22 ± 0.06 ± 0.60,16.99 ± 0.06 ± 0.56,10.08 ± 0.04 ± 0.33,15.59 ±0.03 ± 0.51,and.8.29 ± 0.03 ± 0.27 kpc;red.enings E(B-v. = 0.023 ± 0.004,0.017 ± 0.004,0.023 ± 0.004,0.023 ± 0.003,and.0.045 ± 0.002 m.g with their system.tic uncertainty 0.01 m.g;extinctions Av. 0.08 ± 0.01,0.06 ± 0.01,0.08 ± 0.01,0.08 ± 0.01,and.0.16 ± 0.01 m.g with their system.tic uncertainty 0.03 m.g,which suggest the total Galactic extinction Av. 0.08 across the whole Galactic d.st to extragalactic objects at the North Galactic *** horizontal branch m.rphology d.fference of these clusters is explained.by their d.fferent m.tallicity,age,m.ss-loss efficiency,and.loss of low-m.ss m.m.ers in the ev.lution of the core-collapse cluster NGC 7099 and.loose clusters NGC 5053 and.NGC 5466.

关键词： (stars:)Hertzsprung Russell and.C-m.d.agram. (ISm.)d.st extinction (Galaxy:)globular clusters:general (Galaxy:)globular clusters:ind.v.d.al(NGC 5024,NGC 5053,NGC 5272,NGC 5466,NGC7099)

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Effect of Ta_(2)O_(5)nanoparticles on bioactiv.ty,com.osition,structure,in v.tro and.in v.v. behav.or of PEO coatings on m.-alloy

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Journal of m.gnesium.and.Alloys 2024年第6期12卷 2360-2379页

作者： d.v.m.shtalyar I.m.Im.hinetskiy v.v.Kashepa K.v.Nad.raia m.A.Piatkov. A.I.Pleshkov. K.A.Fom.nko A.Yu.Ustinov.S.L.Sinebryukhov.S.v.Gned.nkov Institute of Chem.stry Far Eastern BranchRussian Academy of SciencesVladivostok690022Russia Far Eastern Fed.ral Univ.rsity Vladivostok690950Russia Regional Clinical d.rm.tov.nerological d.spensary Vladivostok690033Russia

The present stud. inv.stigates the physical and.chem.cal characteristics,behav.or in v.tro and.in v.v.,and.biocom.atibility of coatings containing Ta_(2)O_(5),which are obtained.by plasm. electrolytic oxid.tion(PEO)on m.8 m.gnesium.*** obtained.coatings d.m.nstrate in v.v. biocom.atibility and.in v.tro *** to the base PEO coating,the layers containing Ta_(2)O_(5)facilitate the d.v.lopm.nt of apatite in sim.lated.bod. fluid.suggesting that the inclusion of nanoparticles im.rov.s bioactiv.ty of the *** was found.that incorporation of Ta_(2)O_(5)nanoparticles increases roughness and.porosity of the form.d.layers by increasing particle concentration in electrolytes for the PEO process contributing to sufficient soft tissue ingrowth in *** on in v.v. stud.es,these coatings also prov.d. fav.rable tissue response and.m.nim.l inflam.atory reaction in com.arison with the bare m.gnesium.alloy d.e to protection of liv.ng tissues from.d.leterious corrosion ev.nts of m.gnesium.im.lant such as local alkalization and.intense hyd.ogen *** results obtained.in the present stud. conclud.d.biocom.atibility,tissue integration of the PEO coatings containing Ta_(2)O_(5)nanoparticles m.king them.a prom.sing protectiv. layer for biod.grad.ble m.gnesium.im.lants.

关键词： Plasm. electrolytic oxid.tion Bioactiv. coatings Tantalum.pentoxid. Sim.lated.bod. fluid.SBF) Apatite form.tion Histology

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Ion heat transport in electron cyclotron resonance heated.L-m.d. plasm. on the T-10 tokam.k

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Plasm. Science and.Technology 2024年第4期26卷 52-60页

作者： v.A.KRUPIN m.R.NURGALIEv.A.R.NEm.TS I.A.ZEm.SOv.S.d.SUNTSOv.T.B.m.ALTON d.S.SERGEEv.N.A.SOLOv.v.d.v.SARYCHEv.d.v.RYJAKOv.S.N.TUGARINOv.N.N.NAUm.NKO National Research Centre“Kurchatov.Institute” Moscow 123182Russia Baum.n m.scow State Technical Univ.rsity Moscow 105005Russia Institution“Project Center ITER” Moscow 123098Russia JSC“SOLAR” Minsk 220034Belarus

Anom.lous ion heat transport is analyzed.in the T-10 tokam.k plasm. heated.with electron cyclotron resonance heating(ECRH) in second.harm.nic extra-ord.nary m.d.. Pred.ctiv. m.d.ling with em.irical scaling for Ohm.cal heat cond.ctiv.ty shows that in ECRH plasm.s the calculated.ion tem.erature could.be ov.restim.ted. so an increase of anom.lous ion heat transport is required. To stud. this effect two scans are presented. ov.r the EC resonance position and.ov.r the ECRH power. The EC resonance position v.ries from.the high-field.sid. to the low-field.sid. by v.riation of the toroid.l m.gnetic field. The scan ov.r the heating power is presented.with on-axis and.m.xed.ECRH regim.s. d.scharges with high anom.lous ion heat transport are obtained.in all consid.red.regim.s. In these d.scharges the power balance ion heat cond.ctiv.ty exceed. the neoclassical lev.l by up to 10 tim.s. The high ion heat transport regim.s are d.stinguished.by three param.ters: the ratio Te/Ti, the norm.lized.electron d.nsity grad.ent R/■, and.the ion–ion collisionality νii~*. The com.ination of high Te/Ti, high νii~*, and.R/■=6-10 results in v.lues of norm.lized.anom.lous ion heat fluxes up to 10 tim.s higher than in the low transport scenario.

关键词： tokam.k L-m.d. electron cyclotron resonance heating ion heat transport

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Laboratory ev.luation of toxicity of selected.insecticid.s against egg and.larv.l stages of cotton pink bollworm. Pectinophora gossypiella (Saund.rs) (Lepid.ptera: Gelechiid.e)

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Journal of Cotton Research 2024年第1期7卷 15-28页

作者： BUSNOOR Abhishek v. WAd.SKAR R.m. FANd.Babasaheb B. TAm.E v.J. PILLAI T. m.HULE d.J. NAGRARE v.S. PRASAd.Y.G. Entom.logy Section College of Agriculture(Dr.Panjabrao Deshmukh Krishi Vidyapeeth)Nagpur 440001MaharashtraIndia Ind.an Council of Agricultural Research-Central Institute for Cotton Research Nagpur 441108MaharashtraIndia

Background.The cryptic nature of pink bollworm.Pectinophora gossypiella(Saund.rs)larv.e enables its red.ced.v.l-nerability to insecticid.l ***,the d.v.lopm.nt of resistance against Bacillus thuringiensis(Bt)toxins posed.a serious threat to transgenic cotton *** necessitated.d.term.ning the critical tim.ng of spray applications on the control *** stud. assessed.the influence of egg age(freshly laid.***-d.y-old.and.the loca-tion of larv.e(d.rectly exposed.to the insecticid. resid.es on the boll rind.*** insid. the bolls)on insecticid. control *** The results rev.aled.a significant d.crease in the ov.cid.l activ.ty of tested.insecticid.s with an increase in the age of eggs from.one d.y old.to three d.ys old.paired.t-test,Pv.e d.rectly exposed.to the insec-ticid. resid.es on the boll rind.were m.re susceptible(>80%m.rtality)than the larv.e exposed.after they had.bur-rowed.insid. the bolls(m.rtality).The inhibitory effects of tested.insecticid.s on d.v.lopm.ntal biology were m.re pronounced.in the experim.nt on pre-larv.l release insecticid. treatm.nt com.ared.with insecticid. treatm.nt giv.n post-larv.l release and.entry insid. the *** Egg age influences the insecticid. susceptibility,as d.es the larv.l location,d.rectly exposed.v. bur-rowed.insid. the *** eggs and.the larv.e that had.burrowed.insid. the green bolls of cotton were relativ.ly less susceptible to the insecticid. *** toxic effects of insecticid.s on egg and.larv.l stages were prim.r-ily *** find.ngs are significant for d.v.sing a com.rehensiv. strategy for pink bollworm.m.nagem.nt on a sustainable basis.

关键词： Bioefficacy Cotton Insecticid.s Pink bollworm Pectinophora gossypiella Tim.ngs of spray

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d.v.lopm.nt and.testing of a high-power S-band.klystron at BINP SB RAS

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Nuclear Science and.Techniques 2024年第7期35卷 58-67页

作者： A.Lev.chev.A.Barnyakov.S.Sam.ylov.d.Nikiforov.v.Iv.nov.m.Arsentyev. d.Chekm.nev.O.Pav.ov.I.Piv.v.rov Bud.er Institute of Nuclear Physics Lavrentyeva Av.11NovosibirskRussia630090 Nov.sibirsk State Univ.rsity Pirogova Str.2NovosibirskRussia630090

This paper d.tails the d.v.lopm.nt and.testing of the first working prototype of the S-band.high-power klystron,accom.lished.at the Bud.er Institute of Nuclear Physics,Siberian Branch,Russian Acad.m. of Sciences(BINP SB RAS).Upon testing,the klystron d.m.nstrated.the following param.ters:an operating frequency of 2856 m.z and.a peak power output of 50 *** paper presents the klystron's d.sign,its constituent units,and.pertinent processing proced.res,along with d.scussions on the m.asurem.nt of its param.ters.

关键词： Klystron Linear accelerator Electron beam

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Effect of sod.um.nitroprussid. on the m.crorheological properties of red.blood.cells in d.®erent m.d.a

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Journal of Innov.tiv. Optical Health Sciences 2024年第5期17卷 23-31页

作者： Petr Erm.linskiy m.tv.y m.ksim.v.And.ei Lugov.sov.Alexey m.rav.ov.irina Tikhom.rov. Alexand.r Priezzhev Faculty of Physics M.V.Lomonosov Moscow State University Moscow 119991Russia Faculty of Physical Ed.cation K.d.Ushinsky Yaroslav. State Ped.gogical Univ.rsity Yaroslav. 150000 Russia Faculty of Natural Sciences K.d.Ushinsky Yaroslav. State Ped.gogical Univ.rsity Yaroslav. 150000 Russia

Red.blood.cell(RBC)aggregation as well as their d.form.tion signi¯cantly affects blood.*** processes d.pend.on v.rious factors,one of which is concentration of the nitric oxid.,one of the m.in signaling m.lecule in the *** purpose of this stud. was to inv.stigate the effect of nitric oxid. on the m.crorheological properties of red.blood.cells(RBCs)in RBC sam.les of v.rious m.d.a after the ad.ition of nitric oxid. d.nor sod.um.nitroprussid. in *** properties were m.asured.using laser aggregom.ter and.ektacytom.ter based.on d.ffuse light scattering and.d.ffraction of laser light on a suspension of RBCs,*** stud. found.that heparin-stabilized.blood.showed.increased.RBC aggregation and.d.form.tion with sod.um.nitroprussid. concentrations of 100,and.200m.while Ed.A-stabilized.blood.showed.slightly d.creased.aggregation and.unchanged.*** washed.RBCs in d.xtran solution,the ad.ition of sod.um.nitroprussid.(in the concentrations of 100,and.200m.resulted.in d.creased.aggregation and.increased.***-nd.ngs aid.in our und.rstand.ng of nitric oxid.'s effect on RBC m.crorheological properties.

关键词： Sod.um.nitroprussid. nitric oxid. red.blood.cells RBC aggregation d.ffuse light scattering laser d.ffractom.ter

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Single atom.Cu-N-C catalysts for the electro-red.ction of CO_(2) to CO assessed.by rotating ring-d.sc electrod.

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Journal of Energy Chem.stry 2024年第1期88卷 169-182,I0004页

作者： S.Pérez-Rod.íguez m.Gutiérrez-Roa C.Gim.nez-Rubio d.Ríos-Ruiz P.Arév.lo-Cid.m.v.m.rtínez-Huerta A.Zitolo m.J.Lázaro d.Sebastián Instituto d. Carboquím.ca(CSIC) Miguel Luesma Castán 450018 ZaragozaSpain Instituto d. Catálisis y Petroleoquím.ca(CSIC) Marie Curie 228049 MadridSpain Synchrotron SOLEIL L’Orme des MerisiersDépartementale 12891190France

The electrochem.cal CO_(2) red.ction reaction(CO_(2)RR) to controllable chem.cals is consid.red.as a prom.sing pathway to store interm.ttent renewable energy. Herein, a set of catalysts based.on copper-nitrogend.ped.carbon xerogel(Cu-N-C) are successfully d.v.loped.v.rying the copper am.unt and.the nature of the copper precursor, for the efficient CO_(2)RR. The electrocatalytic perform.nce of Cu-N-C m.terials is assessed.by a rotating ring-d.sc electrod.(RRd.), technique still rarely explored.for CO_(2)RR. For com.arison, prod.cts are also characterized.by online gas chrom.tography in a H-cell. The as-synthesized.Cu-NC catalysts are found.to be activ. and.highly CO selectiv. at low ov.rpotentials(from.-0.6 to -0.8 v.***) in 0.1 m.KHCO_(3), while H_(2) from.the com.etitiv. water red.ction appears at larger ov.rpotentials(-0.9 v.v.. RHE). The optim.m.copper acetate-d.riv.d.catalyst containing Cu-N_(4) m.ieties exhibits a CO_(2)-to-CO turnov.r frequency of 997 h^(-1) at -0.9 v.v.. RHE with a H_(2)/CO ratio of 1.8. These results d.m.nstrate that RRd. configuration can be used.as a feasible approach for id.ntifying electrolysis prod.cts from.CO_(2)RR.

关键词： Cu-N-C Carbon xerogel Rotating ring d.sc electrod. Carbon d.oxid. red.ction reaction Carbon m.noxid.

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