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Soft Nanoscience Letters 2013年第3期3卷 46-51页

作者： Alexander D. Pogrebnjak oleksandr V. Bondar Oleg V. Sobol Vyacheslav M. Beresnev Kharkov National University Kharkov Ukraine Kharkov National Technical University Kharkov Ukraine Sumy State University R.-Korsakov Sumy Ukraine

Using such unique methods of analysis as slow positron beam (SPB), RBS, μ-PIXE (proton microbeam), XRD, SEM with EDS, XPS, nanohardness and elastic modulus measurements, we studied superhard nanostructure Ti-Si-N coatings, which were deposited using Cathodic-PVD method, before and after annealing at the temperature of 600°C for 30 minutes. It is shown in the paper that redistribution of N and Si occurs on the borders of nanograins after annealing, amorphous phase α-SiNx (Si3N4) is created, defects segregates on interfaces and forms vacancy-type clusters with rather high concentration from 5 × 1016 cm-3 to 7.5 × 1017 cm-3 due to thermodiffusion. Solid solution (Ti,Si)N and small concentration of α-SiN (close to XRD detection limits) are formed in the coating. Also it was obtained, that deflected mode is formed in the coating (compressive deformation equals to –2.6%), but after thermal annealing deformation reduces to a value of -2.3%. Size of nanograins of solid solution (Ti, Si)N increases from 12.5 nm to (13.2 ÷ 13.4) nm. 25 nm size grains increase their size to 28.5 nm after annealing (under another deposition regime).

关键词： Nanostructure Wear Nanohardness Profiles of Defects Ti-Si-N

辙叉与钢轨间采用奥氏体钢过渡的闪光焊工艺和设备

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铁道建筑 2017年第12期57卷 102-105页

作者： Kuchuk-Yatsenko.Serhii.Ivanovych Kavunichenko.oleksandr.Vasylovych 马萍张铭达乌克兰国家科学院巴顿电焊研究所基辅03680 珠海启世机械设备股份有限公司广东珠海519085 沈阳铁路局工务处辽宁沈阳110001

道岔相关技术是高速铁路关键技术之一。为提高铁路辙叉的使用寿命,采取了将辙叉和与之连接的钢轨焊连在一起的形式,取代了接头原有的螺栓连接。乌克兰国家科学院巴顿电焊研究所开展了相关研究,研发出了辙叉与钢轨焊接的连续闪光对焊设备... 详细信息

道岔相关技术是高速铁路关键技术之一。为提高铁路辙叉的使用寿命,采取了将辙叉和与之连接的钢轨焊连在一起的形式,取代了接头原有的螺栓连接。乌克兰国家科学院巴顿电焊研究所开展了相关研究,研发出了辙叉与钢轨焊接的连续闪光对焊设备,同时,开发了高锰钢辙叉心与普通钢轨之间插入奥氏体钢过渡段的脉动闪光焊接工艺,焊接接头不需要采取后续热处理即可达到相关要求。

关键词：道岔闪光对焊脉动闪光焊接试验研究辙叉高锰钢奥氏体过渡段微观结构热处理

Effect of hypoxia and glutamine or glucose deprivation on the expression of retinoblastoma and retinoblastoma-related genes in ERN1 knockdown glioma U87 cell line

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American Journal of Molecular Biology 2012年第1期2卷 21-31页

作者： Dmytro O. Minchenko Leonid L. Karbovskyi Serhii V. Danilovskyi Michel Moenner oleksandr H. Minchenko Department of Molecular Biology Palladin Institute of Biochemistry National Academy of Sciences of Ukraine Kyiv Ukraine Department of Molecular Biology Palladin Institute of Biochemistry National Academy of Sciences of Ukraine Kyiv Ukraine INSERM U920 Molecular Mechanisms of Angiogenesis Laboratory University Bordeaux 1 Talence France Department of Molecular Biology Palladin Institute of Biochemistry National Academy of Sciences of Ukraine Kyiv Ukraine National O.O. Bohomoletz Medical University Kyiv Ukraine INSERM U920 Molecular Mechanisms of Angiogenesis Laboratory University Bordeaux 1 Talence France INSERM U920 Molecular Mechanisms of Angiogenesis Laboratory University Bordeaux 1 Talence France

The expression of retinoblastoma and several retinoblastoma-related genes was studied in glioma cell line U87 and its subline with knockdown of ERN1 (endoplasmic reticulum—nuclei-1), the main endoplasmic reticulum stress sensing and signaling enzyme. It was shown that a blockade of the ERN1 enzyme function increases the expression levels of retinoblastoma, retinoblastoma-like 1 and most retinoblastoma related genes: EID1, JARID1B, E2F1, E2F3, RBAP48 and CTIP, does not change RNF40 and RBAP46 and decreases KDM5A. We have also demonstrated that hypoxia reduces the expression levels of retinoblastoma, EID1, and E2F1 in ERN1-deficient glioma cells only. At the same time, the expression levels of retinoblastoma-like 1, E2F3, RBAP46, RBAP48 and CTIP decrease, while JARID1B and RBBP2 increase in both types of cells in hypoxic conditions, but the expression is much stronger in cells with suppressed function of ERN1. The expression level of JARID1B and KDM-5A mRNA is also enhanced in glutamine deprivation condition in both tested cell types, moreover, this effect is amplified by the blockade of the ERN1 enzyme function. The expression levels of retinoblastoma, EID1, RBAP48, and E2F3 are decreased in glutamine deprivation condition only in ERN1-deficient glioma cells, but RBL1, CTIP, RBAP46, and E2F1—in both tested cell types with more significant effect in ERN1-deficient cells. Glucose deprivation condition leads to a decrease of expression levels of retinoblastoma, RBL1, E2F3, RBAP46, and RBAP48 in both used cell types and of EID1 and E2F1 only in glioma cells with suppressed function of signaling enzyme ERN1. Thus, expression levels of retinoblastoma and most retinoblastoma-related genes are increased under a blockade of ERN1 enzyme function and significantly changed in hypoxia, glucose or glutamine deprivation conditions both in control U87 cells and ERN1-deficient cells, but inhibition of the unfolded protein response sensor ERN1 predominantly enhances these effects. Mor

关键词： mRNA Expression Retinoblastoma RBL1 RBAP48 RBAP46 CTIP KDM5A JARID1B E2F1 E2F3 Glioma Cells ERN1 Hypoxia Glucose Deprivation Glutamine Deprivation

Improved Efficiency of ZnO and Ge Purification

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Journal of Crystallization Process and Technology 2019年第3期9卷 39-47页

作者： Grygoriy S. Pekar Alexandr F. Singaеvsky Myhailo M. Lokshin Ilya A. Vasin oleksandr V. Dubikovskyi V. Lashkaryov Institute of Semiconductor Physics National Academy of Sciences of Ukraine Nauki Kyiv Ukraine

Unconventional ways to improve the efficiency of purification of two different semiconductor materials of current interest, ZnO and Ge, are described. It is shown that, by using chemically assisted vapour transport of ZnO with carbon as a transporting agent, the degree of chemical purity of ZnO can be increased by more than an order of magnitude. It is also found that heating of the molten Ge in the experimentally determined narrow (about 20?C wide) temperature range in which an intense evaporation of certain substances is observed, leads to a significant reduction of germanium contamination. As a result, a subsequent deep purification of pre-heat treated germanium by zone refining can be achieved at twice reduced (as compared with “non-treated” Ge) number of passes of a boat with germanium through the melting zones. Thus, the Ge purification process becomes faster, cheaper and more efficient.

关键词： Purification Germanium Zinc Oxide

Effect of Hypoxia on the Expression of a Subset of Proliferation Related Genes in IRE1 Knockdown U87 Glioma Cells

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Advances in Biological Chemistry 2017年第6期7卷 195-210页

作者： Dariia O. Tsymbal Dmytro O. Minchenko Oksana S. Hnatiuk Olha Y. Luzina oleksandr H. Minchenko Department of Molecular Biology Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine Kyiv Ukraine

We have studied the expression of a subset of genes encoding important tumor growth related factors in U87 glioma cells with IRE1 (inositol requiring enzyme-1) knockdown as well as their hypoxic regulation. It was shown that the expression levels of activating transcription factor 6 (ATF6), clusterin (CLU), adhesion G protein-coupled receptor E5 (ADGRE5), transglutaminase?2, C polypeptide (TGM2), leukemia inhibitory factor (LIF), phosphoserine aminotransferase 1 (PSAT1), glyoxalase I (GLO1) and tetraspanin 13 (TSPAN13) are significantly down-regulated in glioma cells with the knockdown of IRE1 signaling enzyme. It was also shown that in glioma cells subjected to hypoxia, the expression levels of PSAT1, TSPAN13, EIF2AK3, and TGM2 genes were up-regulated, whereas the expression of ATF6 gene was down-regulated. At the same time, the expression levels of LIF, CLU, and ADGRE5 genes did not change in response to hypoxic treatment.?Furthermore, inhibition of IRE1, a key effector of an unfolded protein response pathway, modified the effect of hypoxia on the expression of most studied genes. Present study demonstrates that IRE1 knockdown down-regulated the expression of most studied genes and modified their hypoxic regulation and that these changes possibly contributed to the suppression of glioma growth in cells without IRE1 signaling enzyme function.

关键词： IRE1 Knockdown Hypoxia mRNA Expression EIF2AK3 ATF6 CLU PSAT1 TGM2 U87 Glioma Cells

The vascular endothelial growth factor genes expression in glioma U87 cells is dependent from ERN1 signaling enzyme function

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Advances in Biological Chemistry 2012年第2期2卷 198-206页

作者： Dmytro O. Minchenko Kateryna I. Kubaichuk Oksana O. Ratushna Serhiy V. Komisarenko oleksandr H. Minchenko Department of Molecular Biology Palladin Institute of Biochemistry National Academy of Sciences of Ukraine Kiev Ukraine

The expression of different vascular endothelial growth factor (VEGF) genes was studied in glioma U87 cells with endoplasmic reticulum–nuclei-1 (ERN1) loss of function and its regulation by hypoxia and glutamine or glucose deprivation conditions as model of ischemia. The blockade of function of the ERN1 enzyme, which is a major sensor of endoplasmic reticulum stress, leads to a decrease of the VEGFA, VEGFB and VEGFC mRNA expression level. The level of VEGFA proteins also decreases at this experimental condition in the cytosolic fraction, but increases in the nuclear fraction. Hypoxia does not affect VEGFC and increases the expression level of VEGFA and VEGFB mRNA in both used cell types, however, the change was much less profound in cells with suppressed function of ERN1. The expression level of VEGFC mRNA decreases in both used cell types in glutamine deprivation condition, however, the change was more profound in control glioma cells. At the same time, the expression level of VEGFA mRNA increases and VEGFB—decreases in gluta-mine deprivation condition in control glioma cells only. Exposure of glioma cells to glucose deprivation condition increases VEGFB mRNA expression level in both used cell types;however, VEGFA—in control glioma cells only and VEGFC—in cells with ERN1 signaling enzyme loss of function only. Thus, the results of this study clearly demonstrated the down-regulation of the expression of all three VEGF genes in glioma cells with ERN1 loss of function which correlates to the suppressed angiogenesis and proliferation rate of these cells. Moreover, the effect of hy-poxia and glutamine or glucose deprivation condition on the expression level of all VEGF genes is different and mainly depends on ERN1 signaling enzyme function.

关键词： VEGF Expression ERN1 Hypoxia Ischemic Conditions Angiogenesis Glioma U87 Cells

Expression of casein kinase genes in glioma cell line U87: Effect of hypoxia and glucose or glutamine deprivation

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Natural Science 2012年第1期4卷 38-46页

作者： Dmytro O. Minchenko Leonid L. Karbovskyi Serhii V. Danilovskyi Anastasia P. Kharkova oleksandr H. Minchenko 不详

The endoplasmic reticulum-nuclei-1 (ERN1) sensing and signaling enzyme mediates a set of complex intracellular signaling events known as the unfolded protein response. We have studied the effect of hypoxia and ischemic conditions (glucose or glutamine deprivation) on the expression of several casein kinase-1 and -2 genes in glioma U87 cells and its subline with suppressed function of ERN1. It was shown that blockade of ERN1, the key endoplasmic reticulum stress sensor, leads to an increase in the expression levels of casein kinase-1G2, -1E, -2B and NUCKS1 mRNA, but suppresses casein kinase-1A1, -1D and -2A1. Moreover, the expression levels of casein kinase-1A1, -1D and 1G3 as well as casein kinase-2A1 and -2A2 mRNAs are significantly increased under glutamine dep- rivation conditions both in control and ERN1- deficient glioma cells. At the same time, casein kinase-1E, -2B and NUCKS1 mRNA expression levels are also increased under this condition, but only in cells with suppressed function of ERN1. The expression level of NUCKS1 mRNA, however, is decreased both in control glioma cells and in genetically modified cells, but casein kinase-1G2—only in control U87 cells. Cell exposure to glucose deprivation conditions enhances the expression levels of casein kinase- 1D, 1G3, -1E and -2A1 in both types of glioma cells used, but casein kinase-2B expression levels increase only in cells with suppressed function of ERN1. Hypoxia induces or suppresses the expression of most of the studied genes mainly in ERN1-knockdown cells only. Results of this study show that hypoxia as well as glutamine and glucose deprivation conditions change the expression level most of casein kinase genes and that these effects are dependent on ERN1 signaling enzyme function.

关键词： mRNA Expression Casein Kinase 1A 1D 1G2 1G3 1E 2A1 2A2 2B and NUCKS1 Glioma Cells Endoplasmic Reticulum-Nuclei-1 (ERN1 IRE-1α) Hypoxia Glucose and Glutamine Deprivation

Geothermal Energy Production Potential from Oil and Gas Fields in Western Ukraine

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Journal of Geological Resource and Engineering 2019年第4期7卷 123-131页

作者： oleksandr Burachok oleksandr Kondrat Schlumberger Software Integrated Solutions Guenther-Wagner-Allee 1930177Germany Oil and Gas Production Department Institute of Oil and Gas EngineeringIvano-Frankivsk National Technical University of Oil and GasKarpatska Str.15Ivano-Frankivsk 76019Ukraine

Western Ukraine as well as Crimea Peninsula is well known for their geothermal *** classic low enthalpy geothermal project is based on the construction of a binary power plant,and includes recycling of water through one or several doublets of wells;produced hot water is directed to a heat exchanger(vaporizer),in which a secondary(working)fluid with low boiling point and high vapor pressure vaporizes and rotates a turbine to produce *** highest risk for the project is associated with drilling new wells,which may not hit the target or not have the required *** Ukraine is one of the oldest oil and gas production regions in *** majority of the fields are on a late stage of the development that is characterized with high produced volumes of water that after separation is being reinjected back for pressure *** this study,we evaluated the possibility of geothermal energy production,extracted from water that is produced together with oil and gas,based on numerical reservoir simulation models for a typical reservoir setting.

关键词： Western Ukraine geothermal energy energy production potential sensitivity analysis reservoir simulation

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PLAN B地下居住空间

现代装饰 2020年第12期 154-157页

作者： Serhii Makhno Olha Sobchyshyna oleksandr Makhno Ihor Havrylenko oleksandr Bokhan Maryna Hrechko Serhii Filonchuk 舒禹(编译) 乌克兰Sergey Makhno Architects 不详

项目是一个位于地下,深度不超过15米的独立房子,名为"B计划",建筑外观看起来像现代艺术博物馆的入口,占地面积仅172平方米,主要建筑材料为钢筋混凝土,内部设计有足够的舒适性,能与地面的住所媲美。该居住空间具有一定的安全性,且包含了... 详细信息

项目是一个位于地下,深度不超过15米的独立房子,名为"B计划",建筑外观看起来像现代艺术博物馆的入口,占地面积仅172平方米,主要建筑材料为钢筋混凝土,内部设计有足够的舒适性,能与地面的住所媲美。该居住空间具有一定的安全性,且包含了各类必需设施,比如下水道、空气净化、封闭通风系统和消毒区等。该居住空间能为带有两到三个孩子的家庭提供舒适的生活环境,地下空间内有两间主卧室、一间封闭式储藏室、一间浴室、医疗室、睡眠区、健身区和带植物的花园,可以种植蔬菜和水果,且配有食物仓库,用来实现自给自足。

关键词：现代艺术博物馆居住空间占地面积空气净化医疗室建筑外观主要建筑材料主卧室

Interpretations of St. Francis of Assisi in the 20th Century： Philosophical-Anthropological Perspective

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History Research 2015年第1期5卷 12-27页

作者： oleksandr Golozubov National Technica University Kharkiv Ukraine

The article deals with investigating and comparing key modern and postmodern interpretations of St. Francis of Assisi from a philosophical-anthropological perspective. We determine these interpretations to be as follows: a man of wonder and lover of wisdom, in other words, a true philosopher; hero of all times; defender of the poverty and poor; "champion of liberty"; peace-maker and pacifist; God?s troubadour; patron of nature and animals. All of these manifestations of St. Francis? personality have been enriched by his Christocentrism and joyful vision. Moreover, this can be traced in primary sources and also echoes in the late modern period. Postmodernity not only incorporated similar motives into the cultural space with new focus and accent, but it also put forward some new visions. St. Francis was postulated as God?s Fool, not so much in the medieval as in the postmodern sense of foolishness and madness as a way of interaction with the absurd and puzzle-like world in which we live. It also reconciles traditional opposition between joy and laughter as inner feelings and outward emotions within the frame of the postmodern Divine Comedy.

关键词：哲学人类学弗朗西斯后现代主义后现代性文化空间哲学家制造者视野