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A New Bi-enzyme Potentiometric Sensor for Arginine Analysis Based on Recombinant Human Arginase I and Commercial Urease

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材料科学与工程（中英文A版） 2011年第6期1卷 819-827页

作者： Nataliya Stasyuk oleh smutok Galina Gayda Mykhailo Gonchar Yevgen Koval＇chuk Ivan Franko National University Lviv 79005 Ukraine Institute of Cell Biology National Academy of Science of Ukraine Lviv 79005 Ukraine Rzeszow University Kolbuszowa 36100 Poland

Monitoring of L-arginine in serum blood as well as in wines is very essential in medicine and food industry. A potentiometric L-arginine selective bi-enzyme biosensor based on the recombinant human liver arginase I and commercial urease co-immobilised in calcium alginate gel on the surface of ammonium selective electrode has been described. Two arginase preparations were used: free enzyme (E) and covalently immobilized on the surface of gold nanoparticles (ENPs). Some bioanalytical parameters (sensitivity, selectivity and storage stability) of the developed biosensors were investigated. The biosensors exhibit a good response to the target analyte with the linear range from 0.12 to 40 mM for ENPs-based and from 0.50 to 50 mM, for E-based with detection limit below 10-4 M of L-arginine. The ENPs-based biosensor compared with E-based has demonstrated improved physico-chemical characteristics, namely, a higher sensitivity (5-fold) and storage stability (120%). Moreover, both biosensors are sufficiently stable in exploitation and develop a stationary response during 3-5 min (t95%) to the analyte. The constructed E-based biosensor was tested on the real samples of commercial pharmaceuticals products ("Tivortin", "Cytrarginine", "Aminoplazmal 10% E"). The obtained L-arginine content was in a good correlation with that declared by producers.

关键词： L-精氨酸电位传感器商业重组酶生物传感器贮存稳定性电子传感器双酶

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