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Cardiac differentiation is modulated by anti-apoptotic signals in murine embryonic stem cells

World Journal of Stem Cells 2024年第5期16卷 551-559页

作者： Amani Yehya Joseph Azar Mohamad Al-Fares Helene Boeuf Wassim Abou-Kheir Dana Zeineddine ola Hadadeh Department of Anatomy Cell Biology and Physiological SciencesAmerican University of BeirutBeirut 1107-2020Lebanon Inserm BiotisU1026University BordeauxBordeaux F-33000France Rammal Rammal Lab Physio-Toxicity Environmental TeamFaculty of SciencesLebanese UniversityNabatieh 0000Lebanon

BACKGROUND Embryonic stem cells(ESCs)serve as a crucial ex vivo model,representing epiblast cells derived from the inner cell mass of blastocyst-stage embryos.ESCs exhibit a unique combination of self-renewal potency,unlimited proliferation,and pluripotency.The latter is evident by the ability of the isolated cells to differ-entiate spontaneously into multiple cell lineages,representing the three primary embryonic germ layers.Multiple regulatory networks guide ESCs,directing their self-renewal and lineage-specific differentiation.Apoptosis,or programmed cell death,emerges as a key event involved in sculpting and forming various organs and structures ensuring proper embryonic development.How-ever,the molecular mechanisms underlying the dynamic interplay between diffe-rentiation and apoptosis remain poorly understood.AIM To investigate the regulatory impact of apoptosis on the early differentiation of ESCs into cardiac cells,using mouse ESC(mESC)models-mESC-B-cell lym-phoma 2(BCL-2),mESC-PIM-2,and mESC-metallothionein-1(MET-1)-which overexpress the anti-apoptotic genes Bcl-2,Pim-2,and Met-1,respectively.METHODS mESC-T2(wild-type),mESC-BCL-2,mESC-PIM-2,and mESC-MET-1 have been used to assess the effect of potentiated apoptotic signals on cardiac differentiation.The hanging drop method was adopted to generate embryoid bodies(EBs)and induce terminal differentiation of mESCs.The size of the generated EBs was measured in each condition compared to the wild type.At the functional level,the percentage of cardiac differentiation was measured by calculating the number of beating cardiomyocytes in the manipulated mESCs compared to the control.At the molecular level,quantitative reverse transcription-polymerase chain reaction was used to assess the mRNA expression of three cardiac markers:Troponin T,GATA4,and NKX2.5.Additionally,troponin T protein expression was evaluated through immunofluorescence and western blot assays.RESULTS Our findings showed that the upregulation of Bcl-2,Pim-2,and Met-1 genes led to a reduction in th

关键词： Mouse embryonic stem cells Self-renewal Apoptosis Cardiac differentiation B-cell lymphoma 2 PIM-2 Metallothionein-1

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The Antiviral Efficacy of Withania somnifera(Ashwagandha) against Hepatitis C Virus Activity: In Vitroand in SilicoStudy

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Advances in Microbiology 2020年第9期10卷 463-477页

作者： Dina Mofed Wafaa Ahmed Abdel-Rahman Zekri ola said Mohamed Rahouma Ahmed Hassan Ibrahim Faraag Biochemistry and Molecular Biology Unit Department of Cancer Biology National Cancer Institute Cairo University Cairo Egypt Immunity and Virology Unit Department of Cancer Biology National Cancer Institute Cairo University Cairo Egypt Department of Surgical Oncology National Cancer Institute Cairo University Cairo Egypt Department of Botany and Microbiology Faculty of Science Helwan University Cairo Egypt

Objective: Evaluation antiviral effects of Withania somnifera (Ashwagandha) leaf extract against HCV. Methods: cell proliferation was assessed using MTT assay after isolation of lymphocyte cells and treated with Ashwagandha water extract (ASH-WX) (6.25 mg/ml - 100 mg/ml). Assessment of quantitative Real-time PCR, Colony forming assay, TNF-α and molecular docking studies after infection of normal lymphocyte cells with 1 ml (1.5 × 106 HCV) serum then incubated with ASH-WX at concentration 25 mg/ml & 50 mg/ml. Results: MTT assay revealed a significant increase (p α significantly decreased following ASH-WX treatment compared with control untreated infected cells (p 3 IU/mL. Colony formation assay test revealed colony formation reduction compared to positive untreated control. Molecular docking analysis revealed good prediction of binding between Ashwagandha and NS5B and PKN2 compared to Sovaldi. Conclusion: ASH-WX may be a powerful antiviral against HCV infection.

关键词： Antiviral Ashwagandha Hepatitis C Virus Docking Lymphocyte

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Nonlinear Analysis of Organic Polymer Solar Cells Using Differential Quadrature Technique with Distinct and Unique Shape Function

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Computer Modeling in Engineering & Sciences 2023年第12期137卷 2193-2217页

作者： ola Ragb Mokhtar Mohamed Mohamed S.Matbuly Omer Civalek Department of Engineering Mathematics and Physics Faculty of EngineeringZagazig UniversityZagazig44519Egypt Basic Science Department Faculty of EngineeringDelta University for Science and TechnologyGamasa11152Egypt Department of Medical Research China Medical University HospitalChina Medical UniversityTaichung404327TaiwanChina

Four numerical schemes are introduced for the analysis of photocurrent transients in organic photovoltaic devices.Themathematicalmodel for organic polymer solar cells contains a nonlinear diffusion-reaction partial differential equation system with electrostatic convection attached to a kinetic ordinary differential equation.To solve the problem,Polynomial-based differential quadrature,Sinc,and Discrete singular convolution are combined with block marching techniques.These schemes are employed to reduce the problem to a nonlinear algebraic system.The iterative quadrature technique is used to solve the reduced problem.The obtained results agreed with the previous exact one and the finite element method.Further,the effects of different times,different mobilities,different densities,different geminate pair distances,different geminate recombination rate constants,different generation efficiencies,and supporting conditions on photocurrent have been analyzed.The novelty of this paper is that these schemes for photocurrent transients in organic polymer solar cells have never been presented before,so the results may be useful for improving the performance of solar cells.

关键词： Sinc differential quadrature block marching organic solar cells discrete singular convolution

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Population increase impacts the climate,using the sensitive Arctic as an example

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Atmospheric and Oceanic Science Letters 2022年第2期15卷 71-74页

作者： ola M.Johannessen Elena V.Shalina Nansen Scientific Society BergenNorway Nansen International Environmental and Remote Sensing Centre St.PetersburgRussia Institute of Earth Sciences St.Petersburg State UniversityRussia

The global population during the last 100 years has increased from 2 to 7.7 billion,causing an increase in greenhouse gases in the atmosphere.In order to see how population increase is directly related to physical variables of the climate,this Perspective article places observations and scenarios of climate change into context and puts forth a statistical modeling study on how the sensitive Arctic climate responds to the increasing population.The relationships between population,Arctic sea-ice extent(SIE),and surface air temperature(SAT)are very strong,with the increasing population explaining 96%of the decreasing SIE and about 80%of the increasing SAT in the Arctic.Our projection for the SIE using the population as a“proxy predictor”for a projected population of 10 billion people on the Earth in 2100,yields a SIE of 9.30 and 8.21 million km 2 for a linear and squared relationship,respectively,indicating no“tipping point”for the annual ice extent in this century.This adds another dimension to climate understanding for the public at large using population as a proxy variable,instead of the more abstract CO_(2)parameter.This also indicates that it is important to attempt to limit the ongoing increase in population,which is the main cause of the greenhouse gas emissions,in addition to reducing per capita emissions by an exponential increase in implementing renewable energy,a formidable challenge in this century.

关键词： Population Climate Sea ice Arctic

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Will the summer sea ice in the Arctic reach a tipping point?

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Atmospheric and Oceanic Science Letters 2023年第3期16卷 58-62页

作者： ola M.Johannessen Elena V.Shalina Nansen Scientific Society BergenNorway Nansen International Environmental and Remote Sensing Centre St PetersburgRussia

The Arctic sea-ice cover has decreased in extent,area,and thickness over the last six decades.Most global climate models project that the summer sea-ice extent(SIE)will decline to less than 1 million(mill.)km^(2) in this century,ranging from 2030 to the end of the century,indicating large uncertainty.However,some models,using the same emission scenarios as required by the Paris Agreement to keep the global temperature below 2°C,indicate that the SIE could be about 2 mill.km^(2) in 2100 but with a large uncertainty of±1.5 mill.km^(2).Here,the authors take another approach by exploring the direct relationship between the SIE and atmospheric CO_(2) concentration for the summer-fall months.The authors correlate the SIE and In(CO_(2)/CO_(2)r)during the period 1979-2022,where CO_(2)r is the reference value in 1979.Using these transient regression equations with an R2 between 0.78 and 0.87,the authors calculate the value that the CO_(2) concentration needs to reach for zero SIE.The results are that,for July,the CO_(2) concentration needs to reach 691±16.5 ppm,for August 604±16.5 ppm,for September 563±17.5 ppm,and for October 620±21 ppm.These values of CO_(2)for an ice-free Arctic are much higher than the targets of the Paris Agreement,which are 450 ppm in 2060 and 425 ppm in 2100,under the IPCC SSP1-2.6 scenario.If these targets can be reached or even almost reached,the "no tipping point"hypothesis for the summer SIE may be valid.

关键词： Sea ice Arctic Climate change Tipping point

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Determinants of mortality among seniors acutely readmitted for heart failure: racial disparities and clinical correlations

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Journal of Geriatric Cardiology 2022年第9期19卷 719-724页

作者： Tuoyo O Mene-Afejuku Gini P Jeyashanmugaraja Mahfuz Hoq olatunde ola Amit J Shah Department of Cardiology Tower Health SystemReading HospitalPAUSA Yale New Haven Health System Bridgeport hospitalCTUSA Mayo Clinic Health System La CrosseWIUSA Department of Cardiology Emory UniversityAtlantaGAUSA

Heart failure(HF)is a devastating condi-tion characterized by a high rate of mor-tality.[1]About 6.2 million individuals are grappling with the burden of HF in the United States(U.S).[2]Of this over 6 million individuals af-fected with HF,a higher proportion is made up of people older than 65 years.[3,4]More than 50%of pa-tients hospitalized due HF are older than 75 years.[5]Seniors do not just account for the greater propor-tion of individuals affected by HF but also have a worse outcome compared to younger individuals with HF.[6−14]In the light of the above,we aim to address racial disparities as they affect seniors with HF in terms of mortality in addition to other potential prognostic indices.

关键词： admitted mortality clinical

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Solriamfetol impurities:Synthesis,characterization,and analytical method(UPLC-UV)validation

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Journal of Pharmaceutical Analysis 2023年第4期13卷 403-411页

作者： Nafisah Al-Rifai Anas Alshishani Fouad Darras ola Taha Shereen Abu-Jalloud Lena Shaghlil Yousef Al-Ebini Pharmaceutical and Chemical Engineering Department School of Medical SciencesGerman Jordanian UniversityAmmanJordan Faculty of Pharmacy Zarqa UniversityZarqaJordan Research and Development Department Resonance Research LabAmmanJordan Department of Pharmaceutics College of PharmacyKing Khalid UniversityAbhaSaudi Arabia

Given that impurities may affect the quality and safety of drug products,impurity identification and profiling is an integral part of drug quality control and is particularly important for newly developed medications such as solriamfetol,which is used to treat excessive daytime sleepiness.Although the highperformance liquid chromatography analysis of commercial solriamfetol has revealed the presence of several impurities,their synthesis,structure elucidation,and chromatographic determination have not been reported yet.To bridge this gap,we herein identified,synthesized,and isolated eight processrelated solriamfetol impurities,characterized them using spectroscopic and chromatographic techniques,and proposed plausible mechanisms of their formation.Moreover,we developed and validated a prompt impurity analysis method based on ultrahigh-performance liquid chromatography with UV detection,revealing that its selectivity,linearity,accuracy,precision,and quantitation limit meet the acceptance criteria of method validation stipulated by the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use.Thus,the developed method was concluded to be suitable for the routine analysis of solriamfetol substances.

关键词： Solriamfetol Impurity analysis Impurity synthesis UPLC Method validation

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Quantization of Time Independent Damping Systems Using WKB Approximation

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Journal of Applied Mathematics and Physics 2023年第9期11卷 2615-2620页

作者： ola A. Jarab’ah Department of Applied Physics Tafila Technical University Tafila Jordan

In this work time independent damping systems are studied using Lagrangian and Hamiltonian for time independent damping, which are present through the factor eλq. The Hamilton Jacobi equation is formulated to find the Hamilton Jacobi function S using separation of variables technique. We can form this function in compact form of two parts the first part as a function of coordinate q, and the second part as a function of time t. Finally, we find the ability of these systems to quantize through an illustrative example.

关键词： Quantization Hamilton Jacobi Equation Hamilton Jacobi Function Momentum

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Lagrangian Formulation of Fractional Nonholonomic Constrained Damping Systems

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Advances in Pure Mathematics 2023年第9期13卷 552-558页

作者： ola A. Jarab’ah Department of Applied Physics Tafila Technical University Tafila Jordan

Fractional Euler Lagrange equations for fractional nonholonomic constrained damping systems have been presented. The equations of motion are obtained using fractional Euler Lagrange equations in a similar manner to the usual technique. The results of fractional method reduce to those obtained from classical method when μ →0 and α,β →1 are equal unity only. This work is discussed using illustrative example.

关键词： Euler-Lagrange Equations Nonholonomic Constraints Generalized Momenta

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Path Integral Quantization of Non-Natural Lagrangian

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应用数学与应用物理(英文) 2023年第10期11卷 2932-2937页

作者： ola A. Jarab’ah Department of Applied Physics Tafila Technical University Tafila Jordan

Path integral technique is discussed using Hamilton Jacobi method. The Hamilton Jacobi function of non-natural Lagrangian is obtained using separation of variables method. This function makes an important role in path integral quantization. The path integral is obtained as integration over the canonical phase space coordinates, which contains the generalized coordinate q and the generalized momentum p. One illustrative example is considered to explain the application of our formalism.

关键词： Path Integral Quantization Hamilton Jacobi Equation Non-Natural Lagrangian Hamilton Jacobi Function

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