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中国畜牧兽医学会动物营养学分会第八届全国会员代表大会暨...

作者： Tanaka Sachi Watanabe Kouichi ohwada shyuichi Aso Hisashi Yamaguchi Takahiro College of of life science ohoku UniversityJapan College of of life science Tohoku UniversityJapan

＜正＞Monocytes and macropHages have numerous important functions on the immune defense system,because they phagocytose foreign antigens,regulate immunocompetent cells and produce several kinds of *** innate immunity that is crucial for more rapid defense mechanisms is initiated by the recognition of pathogens on germ-line encoded pattern recognition *** recognition is mainly attributed to the family of Toll-like re-

关键词： Expression Monocytes Monocyte Calves

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Prion Protein Binds to Aldolase A Produced by Bovine Intestinal M Cells

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Open Journal of Veterinary Medicine 2015年第3期5卷 43-60页

作者： Yuya Nagasawa Yu Takahashi Wataru Itani Hitoshi Watanabe Yusuke Hidaka Shotaro Morita Kei Suzuki Kouichi Watanabe shyuichi ohwada Haruki Kitazawa Morikazu Imamura Takashi Yokoyama Motohiro Horiuchi Suehiro Sakaguchi Shirou Mohri Michael T. Rose Tomonori Nochi Hisashi Aso Cellular Biology Laboratory Tohoku University Sendai Japan Food Immunology Group Tohoku University Sendai Japan Prion Disease Research Center National Institute of Animal Health Tsukuba Japan Laboratory of Veterinary Hygiene Hokkaido University Sapporo Japan Institute for Enzyme Research The University of Tokushima Tokushima Japan Institute of Biological Environmental and Rural Sciences Aberystwyth University Aberystwyth UK

Microfold (M) cells are a kind of intestinal epithelial cell in the follicle-associated epithelium (FAE) of Peyer’s patches. They can transport antigens and microorganisms to lymphoid tissues. Bovine spongiform encephalopathy (BSE) is a fatal neurodegenerative disorder in cattle. It is linked to variant Creutzfeldt-Jakob disease in humans. Although it is thought that M cells transport the BSE agent, the exact mechanism by which it crosses the intestinal barrier is not clear. We have bovine intestinal epithelial cell line (BIE cells), which can differentiate into the M cell type in vitro after stimulation, and which is able to transport the BSE agent. We show here that M cells are able to incorporate large numbers of PrP coated magnetic particles into intracellular vesicles, which we collected. The results of 2-DE show a specific protein associated with the PrP-coated particles, compared with non-coated particles. This protein was identified as aldolase A, a glycolytic pathway enzyme, using LC-MS/MS analysis. Aldolase A was synthesized and secreted by BIE cells, and increased during M cell differentiation. In the villi of the bovine intestine, aldolase A was detected on the surface of the epithelium and in the mucus droplet of goblet cells. In the FAE of bovine jejunal and ileal Peyer’s patches, aldolase A was localized on the surface and the apical part of the M cells. The binding of rbPrP to aldolase A was clearly detected and inhibited by pre-treatment of anti-aldolase A antibody. Aldolase A was co-stained with incorporated PrPSc in M-BIE cells. These results suggest that bovine M cells and goblet cells synthesize aldolase A, and that aldolase A may have the ability to bind PrP and associate with PrP in cellular vesicles. Therefore, aldolase A-positive M cells may play a key role in the invasion of BSE into the body.

关键词： Peyer’s Patch M Cell BIE Cells Aldolase A PrP Binding Protein

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